tRNA:m(4)X modification enzyme TRM13

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

methyltransferase domain containing protein, putative

Species:

Leishmania braziliensis

UniProt:

A4HDB1_LEIBR

TriTrypDb:

LbrM.24.0550 , LBRM2903_240011200 , LBRM2903_250009200

Length:

399

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 5
NetGPI	no	yes: 0, no: 5

Could not find GO cellular_component term for this entry.

Expansion

Sequence features

A4HDB1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HDB1

Function

Biological processes
Term	Name	Level	Count
GO:0001510	RNA methylation	4	1
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006396	RNA processing	6	1
GO:0006399	tRNA metabolic process	7	1
GO:0006400	tRNA modification	6	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008033	tRNA processing	8	1
GO:0008152	metabolic process	1	1
GO:0009451	RNA modification	5	1
GO:0009987	cellular process	1	1
GO:0016070	RNA metabolic process	5	1
GO:0030488	tRNA methylation	5	1
GO:0032259	methylation	2	1
GO:0034470	ncRNA processing	7	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0034660	ncRNA metabolic process	6	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0043414	macromolecule methylation	3	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044260	obsolete cellular macromolecule metabolic process	3	1
GO:0046483	heterocycle metabolic process	3	1
GO:0071704	organic substance metabolic process	2	1
GO:0090304	nucleic acid metabolic process	4	1
GO:1901360	organic cyclic compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	6
GO:0005488	binding	1	6
GO:0008168	methyltransferase activity	4	6
GO:0008171	O-methyltransferase activity	5	6
GO:0008173	RNA methyltransferase activity	4	6
GO:0008175	tRNA methyltransferase activity	5	6
GO:0016740	transferase activity	2	6
GO:0016741	transferase activity, transferring one-carbon groups	3	6
GO:0043167	ion binding	2	6
GO:0043169	cation binding	3	6
GO:0046872	metal ion binding	4	6
GO:0062105	RNA 2'-O-methyltransferase activity	5	6
GO:0106050	tRNA 2'-O-methyltransferase activity	6	6
GO:0140098	catalytic activity, acting on RNA	3	6
GO:0140101	catalytic activity, acting on a tRNA	4	6
GO:0140640	catalytic activity, acting on a nucleic acid	2	6

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	18	22	PF00656	0.294
CLV_NRD_NRD_1	185	187	PF00675	0.649
CLV_NRD_NRD_1	188	190	PF00675	0.596
CLV_NRD_NRD_1	304	306	PF00675	0.311
CLV_NRD_NRD_1	333	335	PF00675	0.311
CLV_NRD_NRD_1	369	371	PF00675	0.218
CLV_PCSK_FUR_1	186	190	PF00082	0.412
CLV_PCSK_KEX2_1	185	187	PF00082	0.581
CLV_PCSK_KEX2_1	188	190	PF00082	0.503
CLV_PCSK_KEX2_1	304	306	PF00082	0.311
CLV_PCSK_KEX2_1	333	335	PF00082	0.311
CLV_PCSK_KEX2_1	369	371	PF00082	0.218
CLV_PCSK_PC7_1	181	187	PF00082	0.601
CLV_PCSK_SKI1_1	181	185	PF00082	0.615
CLV_PCSK_SKI1_1	212	216	PF00082	0.311
CLV_PCSK_SKI1_1	87	91	PF00082	0.342
CLV_Separin_Metazoa	227	231	PF03568	0.511
DOC_CKS1_1	113	118	PF01111	0.425
DOC_CKS1_1	382	387	PF01111	0.511
DOC_CYCLIN_RxL_1	66	77	PF00134	0.337
DOC_CYCLIN_RxL_1	93	104	PF00134	0.303
DOC_MAPK_gen_1	369	375	PF00069	0.446
DOC_MAPK_gen_1	98	105	PF00069	0.304
DOC_PP1_RVXF_1	196	203	PF00149	0.511
DOC_PP1_RVXF_1	67	74	PF00149	0.338
DOC_PP2B_PxIxI_1	238	244	PF00149	0.511
DOC_USP7_MATH_1	162	166	PF00917	0.540
DOC_USP7_MATH_1	25	29	PF00917	0.277
DOC_USP7_MATH_1	276	280	PF00917	0.418
DOC_USP7_MATH_1	303	307	PF00917	0.511
DOC_USP7_MATH_1	31	35	PF00917	0.306
DOC_USP7_MATH_1	59	63	PF00917	0.340
DOC_USP7_MATH_1	80	84	PF00917	0.546
DOC_WW_Pin1_4	112	117	PF00397	0.419
DOC_WW_Pin1_4	381	386	PF00397	0.511
LIG_14-3-3_CanoR_1	135	144	PF00244	0.376
LIG_14-3-3_CanoR_1	189	195	PF00244	0.329
LIG_14-3-3_CanoR_1	201	207	PF00244	0.425
LIG_14-3-3_CanoR_1	280	288	PF00244	0.511
LIG_BIR_III_2	204	208	PF00653	0.511
LIG_BRCT_BRCA1_1	33	37	PF00533	0.330
LIG_deltaCOP1_diTrp_1	283	288	PF00928	0.418
LIG_FHA_1	211	217	PF00498	0.418
LIG_FHA_1	62	68	PF00498	0.282
LIG_FHA_2	16	22	PF00498	0.354
LIG_FHA_2	73	79	PF00498	0.299
LIG_Integrin_isoDGR_2	127	129	PF01839	0.368
LIG_LIR_Gen_1	104	113	PF02991	0.351
LIG_LIR_Gen_1	255	263	PF02991	0.418
LIG_LIR_Gen_1	347	358	PF02991	0.511
LIG_LIR_Nem_3	104	108	PF02991	0.327
LIG_LIR_Nem_3	122	126	PF02991	0.393
LIG_LIR_Nem_3	196	200	PF02991	0.418
LIG_LIR_Nem_3	255	260	PF02991	0.418
LIG_LIR_Nem_3	347	353	PF02991	0.511
LIG_MLH1_MIPbox_1	33	37	PF16413	0.330
LIG_NRBOX	66	72	PF00104	0.284
LIG_Pex14_1	284	288	PF04695	0.418
LIG_PTB_Apo_2	256	263	PF02174	0.418
LIG_PTB_Phospho_1	256	262	PF10480	0.418
LIG_SH2_CRK	123	127	PF00017	0.384
LIG_SH2_CRK	257	261	PF00017	0.418
LIG_SH2_CRK	38	42	PF00017	0.286
LIG_SH2_STAP1	371	375	PF00017	0.492
LIG_SH2_STAT3	262	265	PF00017	0.511
LIG_SH2_STAT3	371	374	PF00017	0.418
LIG_SH2_STAT5	257	260	PF00017	0.511
LIG_SH2_STAT5	262	265	PF00017	0.483
LIG_SH2_STAT5	378	381	PF00017	0.511
LIG_SH2_STAT5	9	12	PF00017	0.288
LIG_SH3_2	207	212	PF14604	0.511
LIG_SH3_3	110	116	PF00018	0.437
LIG_SH3_3	153	159	PF00018	0.456
LIG_SH3_3	204	210	PF00018	0.511
LIG_SH3_3	240	246	PF00018	0.511
LIG_SH3_3	379	385	PF00018	0.511
LIG_SUMO_SIM_anti_2	60	69	PF11976	0.299
LIG_TRFH_1	242	246	PF08558	0.418
LIG_TYR_ITIM	121	126	PF00017	0.390
LIG_WRC_WIRS_1	102	107	PF05994	0.327
LIG_WRC_WIRS_1	194	199	PF05994	0.418
MOD_CDK_SPK_2	381	386	PF00069	0.511
MOD_CDK_SPxxK_3	381	388	PF00069	0.511
MOD_CK1_1	104	110	PF00069	0.339
MOD_CK1_1	279	285	PF00069	0.418
MOD_CK1_1	306	312	PF00069	0.511
MOD_CK2_1	288	294	PF00069	0.511
MOD_CK2_1	40	46	PF00069	0.285
MOD_GlcNHglycan	138	141	PF01048	0.370
MOD_GlcNHglycan	222	225	PF01048	0.311
MOD_GlcNHglycan	27	30	PF01048	0.354
MOD_GlcNHglycan	305	308	PF01048	0.306
MOD_GlcNHglycan	334	337	PF01048	0.245
MOD_GlcNHglycan	42	45	PF01048	0.252
MOD_GSK3_1	173	180	PF00069	0.512
MOD_GSK3_1	284	291	PF00069	0.511
MOD_GSK3_1	332	339	PF00069	0.459
MOD_GSK3_1	340	347	PF00069	0.466
MOD_N-GLC_1	284	289	PF02516	0.311
MOD_N-GLC_2	387	389	PF02516	0.311
MOD_NEK2_1	190	195	PF00069	0.355
MOD_NEK2_1	220	225	PF00069	0.588
MOD_NEK2_1	288	293	PF00069	0.418
MOD_NEK2_1	30	35	PF00069	0.457
MOD_NEK2_2	284	289	PF00069	0.511
MOD_NEK2_2	31	36	PF00069	0.317
MOD_NEK2_2	82	87	PF00069	0.303
MOD_NMyristoyl	1	7	PF02799	0.405
MOD_PIKK_1	107	113	PF00454	0.361
MOD_PIKK_1	88	94	PF00454	0.349
MOD_PKA_2	136	142	PF00069	0.380
MOD_PKA_2	200	206	PF00069	0.511
MOD_PKA_2	276	282	PF00069	0.497
MOD_PKA_2	303	309	PF00069	0.511
MOD_PKA_2	332	338	PF00069	0.511
MOD_PKB_1	179	187	PF00069	0.600
MOD_Plk_1	284	290	PF00069	0.511
MOD_Plk_1	31	37	PF00069	0.329
MOD_Plk_4	101	107	PF00069	0.324
MOD_Plk_4	151	157	PF00069	0.557
MOD_Plk_4	284	290	PF00069	0.418
MOD_Plk_4	31	37	PF00069	0.433
MOD_Plk_4	5	11	PF00069	0.473
MOD_ProDKin_1	112	118	PF00069	0.415
MOD_ProDKin_1	381	387	PF00069	0.511
TRG_ENDOCYTIC_2	123	126	PF00928	0.390
TRG_ENDOCYTIC_2	257	260	PF00928	0.418
TRG_ENDOCYTIC_2	360	363	PF00928	0.511
TRG_ENDOCYTIC_2	38	41	PF00928	0.294
TRG_ER_diArg_1	179	182	PF00400	0.609
TRG_ER_diArg_1	185	188	PF00400	0.520
TRG_ER_diArg_1	369	371	PF00400	0.434
TRG_Pf-PMV_PEXEL_1	230	235	PF00026	0.431

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A3Q8IG18	Leishmania donovani	77%	86%
A4I0P4	Leishmania infantum	77%	86%
E9AWP4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	79%	86%
Q4QAS7	Leishmania major	77%	86%