Fumarate hydratase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Fumarate hydratase

Gene product:

fumarate hydratase, putative

Species:

Leishmania braziliensis

UniProt:

A4HD88_LEIBR

TriTrypDb:

LbrM.24.0320 , LBRM2903_240008200

Length:

549

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 4
Forrest at al. (procyclic)	no	yes: 4
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 24

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 5

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 10

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 13
NetGPI	no	yes: 0, no: 13

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0005777	peroxisome	6	1
GO:0005829	cytosol	2	1
GO:0020015	glycosome	7	1
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	1
GO:0042579	microbody	5	1
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0097014	ciliary plasm	5	1
GO:0099568	cytoplasmic region	3	1
GO:0110165	cellular anatomical entity	1	2
GO:0005739	mitochondrion	5	1

Expansion

Sequence features

A4HD88

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HD88

PDB structure(s): 6mso_A , 6mso_B , 6mso_C , 6mso_D

Function

Biological processes
Term	Name	Level	Count
GO:0006091	generation of precursor metabolites and energy	3	13
GO:0008152	metabolic process	1	14
GO:0009987	cellular process	1	14
GO:0044237	cellular metabolic process	2	14
GO:0006082	organic acid metabolic process	3	2
GO:0006106	fumarate metabolic process	7	2
GO:0006108	malate metabolic process	7	2
GO:0019752	carboxylic acid metabolic process	5	2
GO:0043436	oxoacid metabolic process	4	2
GO:0043648	dicarboxylic acid metabolic process	6	2
GO:0044281	small molecule metabolic process	2	2
GO:0071704	organic substance metabolic process	2	2
GO:0006099	tricarboxylic acid cycle	3	1
GO:0044238	primary metabolic process	2	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	14
GO:0004333	fumarate hydratase activity	5	14
GO:0005488	binding	1	14
GO:0016829	lyase activity	2	14
GO:0016835	carbon-oxygen lyase activity	3	14
GO:0016836	hydro-lyase activity	4	14
GO:0043167	ion binding	2	14
GO:0043169	cation binding	3	14
GO:0046872	metal ion binding	4	14
GO:0051536	iron-sulfur cluster binding	3	14
GO:0051539	4 iron, 4 sulfur cluster binding	4	14
GO:0051540	metal cluster binding	2	14
GO:0005515	protein binding	2	2
GO:0042802	identical protein binding	3	2
GO:0042803	protein homodimerization activity	4	2
GO:0046983	protein dimerization activity	3	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	87	91	PF00656	0.372
CLV_NRD_NRD_1	217	219	PF00675	0.314
CLV_NRD_NRD_1	3	5	PF00675	0.555
CLV_NRD_NRD_1	79	81	PF00675	0.403
CLV_PCSK_KEX2_1	3	5	PF00082	0.555
CLV_PCSK_SKI1_1	201	205	PF00082	0.388
CLV_PCSK_SKI1_1	209	213	PF00082	0.341
CLV_PCSK_SKI1_1	218	222	PF00082	0.320
CLV_PCSK_SKI1_1	252	256	PF00082	0.372
CLV_PCSK_SKI1_1	284	288	PF00082	0.427
CLV_PCSK_SKI1_1	29	33	PF00082	0.442
CLV_PCSK_SKI1_1	3	7	PF00082	0.411
CLV_PCSK_SKI1_1	332	336	PF00082	0.391
CLV_PCSK_SKI1_1	412	416	PF00082	0.353
CLV_PCSK_SKI1_1	479	483	PF00082	0.372
CLV_PCSK_SKI1_1	80	84	PF00082	0.320
DEG_APCC_DBOX_1	331	339	PF00400	0.410
DEG_Nend_Nbox_1	1	3	PF02207	0.535
DEG_SCF_FBW7_1	5	10	PF00400	0.395
DEG_SCF_FBW7_2	364	371	PF00400	0.229
DOC_CKS1_1	365	370	PF01111	0.451
DOC_MAPK_gen_1	151	161	PF00069	0.452
DOC_MAPK_gen_1	337	347	PF00069	0.320
DOC_MAPK_MEF2A_6	154	163	PF00069	0.320
DOC_MAPK_MEF2A_6	340	347	PF00069	0.229
DOC_MAPK_MEF2A_6	366	374	PF00069	0.320
DOC_MAPK_MEF2A_6	454	461	PF00069	0.320
DOC_PP4_FxxP_1	83	86	PF00568	0.332
DOC_USP7_MATH_1	16	20	PF00917	0.488
DOC_USP7_MATH_1	199	203	PF00917	0.451
DOC_USP7_MATH_1	403	407	PF00917	0.367
DOC_USP7_MATH_1	443	447	PF00917	0.383
DOC_USP7_MATH_1	86	90	PF00917	0.368
DOC_USP7_UBL2_3	20	24	PF12436	0.541
DOC_USP7_UBL2_3	215	219	PF12436	0.334
DOC_USP7_UBL2_3	268	272	PF12436	0.451
DOC_USP7_UBL2_3	387	391	PF12436	0.243
DOC_WW_Pin1_4	3	8	PF00397	0.575
DOC_WW_Pin1_4	364	369	PF00397	0.401
DOC_WW_Pin1_4	435	440	PF00397	0.303
LIG_14-3-3_CanoR_1	154	161	PF00244	0.372
LIG_14-3-3_CanoR_1	315	321	PF00244	0.372
LIG_14-3-3_CanoR_1	376	385	PF00244	0.403
LIG_14-3-3_CanoR_1	393	397	PF00244	0.309
LIG_14-3-3_CanoR_1	72	79	PF00244	0.503
LIG_Actin_WH2_2	140	156	PF00022	0.231
LIG_Actin_WH2_2	64	82	PF00022	0.326
LIG_AP2alpha_1	458	462	PF02296	0.320
LIG_AP2alpha_2	456	458	PF02296	0.339
LIG_APCC_ABBA_1	161	166	PF00400	0.372
LIG_APCC_ABBA_1	455	460	PF00400	0.320
LIG_BRCT_BRCA1_1	187	191	PF00533	0.367
LIG_BRCT_BRCA1_1	443	447	PF00533	0.372
LIG_BRCT_BRCA1_1	60	64	PF00533	0.475
LIG_deltaCOP1_diTrp_1	522	532	PF00928	0.372
LIG_FHA_1	117	123	PF00498	0.365
LIG_FHA_1	365	371	PF00498	0.482
LIG_FHA_1	395	401	PF00498	0.372
LIG_FHA_1	4	10	PF00498	0.400
LIG_FHA_2	243	249	PF00498	0.362
LIG_FHA_2	265	271	PF00498	0.367
LIG_FHA_2	274	280	PF00498	0.372
LIG_LIR_Gen_1	10	18	PF02991	0.567
LIG_LIR_Gen_1	186	193	PF02991	0.380
LIG_LIR_Gen_1	341	352	PF02991	0.229
LIG_LIR_Gen_1	456	465	PF02991	0.372
LIG_LIR_Gen_1	544	549	PF02991	0.461
LIG_LIR_Gen_1	61	71	PF02991	0.367
LIG_LIR_Gen_1	92	100	PF02991	0.357
LIG_LIR_Nem_3	10	15	PF02991	0.574
LIG_LIR_Nem_3	186	192	PF02991	0.362
LIG_LIR_Nem_3	25	31	PF02991	0.346
LIG_LIR_Nem_3	276	280	PF02991	0.444
LIG_LIR_Nem_3	341	347	PF02991	0.229
LIG_LIR_Nem_3	456	461	PF02991	0.372
LIG_LIR_Nem_3	544	549	PF02991	0.363
LIG_LIR_Nem_3	61	67	PF02991	0.451
LIG_LIR_Nem_3	92	97	PF02991	0.320
LIG_PCNA_yPIPBox_3	375	387	PF02747	0.250
LIG_Pex14_2	458	462	PF04695	0.320
LIG_SH2_CRK	187	191	PF00017	0.334
LIG_SH2_CRK	28	32	PF00017	0.476
LIG_SH2_CRK	432	436	PF00017	0.372
LIG_SH2_STAP1	155	159	PF00017	0.372
LIG_SH2_STAP1	187	191	PF00017	0.445
LIG_SH2_STAP1	262	266	PF00017	0.439
LIG_SH2_STAP1	304	308	PF00017	0.451
LIG_SH2_STAT5	275	278	PF00017	0.433
LIG_SH2_STAT5	492	495	PF00017	0.372
LIG_SH3_3	208	214	PF00018	0.320
LIG_SH3_3	35	41	PF00018	0.429
LIG_SH3_4	268	275	PF00018	0.451
LIG_SH3_4	419	426	PF00018	0.447
LIG_SUMO_SIM_anti_2	34	40	PF11976	0.416
MOD_CK1_1	19	25	PF00069	0.490
MOD_CK2_1	19	25	PF00069	0.476
MOD_CK2_1	242	248	PF00069	0.362
MOD_GlcNHglycan	106	109	PF01048	0.454
MOD_GlcNHglycan	122	125	PF01048	0.394
MOD_GlcNHglycan	197	200	PF01048	0.363
MOD_GlcNHglycan	230	233	PF01048	0.367
MOD_GlcNHglycan	245	248	PF01048	0.378
MOD_GlcNHglycan	328	331	PF01048	0.372
MOD_GlcNHglycan	405	408	PF01048	0.445
MOD_GlcNHglycan	443	446	PF01048	0.372
MOD_GSK3_1	116	123	PF00069	0.333
MOD_GSK3_1	19	26	PF00069	0.431
MOD_GSK3_1	195	202	PF00069	0.399
MOD_GSK3_1	243	250	PF00069	0.415
MOD_GSK3_1	3	10	PF00069	0.597
MOD_GSK3_1	392	399	PF00069	0.319
MOD_GSK3_1	443	450	PF00069	0.389
MOD_GSK3_1	461	468	PF00069	0.287
MOD_GSK3_1	475	482	PF00069	0.298
MOD_GSK3_1	54	61	PF00069	0.420
MOD_GSK3_1	71	78	PF00069	0.269
MOD_N-GLC_1	103	108	PF02516	0.420
MOD_N-GLC_1	199	204	PF02516	0.399
MOD_N-GLC_1	475	480	PF02516	0.372
MOD_NEK2_1	153	158	PF00069	0.376
MOD_NEK2_1	179	184	PF00069	0.306
MOD_NEK2_1	185	190	PF00069	0.305
MOD_NEK2_1	205	210	PF00069	0.320
MOD_NEK2_1	227	232	PF00069	0.451
MOD_NEK2_1	394	399	PF00069	0.365
MOD_NEK2_1	461	466	PF00069	0.445
MOD_NEK2_1	58	63	PF00069	0.481
MOD_NEK2_1	70	75	PF00069	0.451
MOD_NEK2_2	247	252	PF00069	0.372
MOD_PIKK_1	153	159	PF00454	0.372
MOD_PKA_2	153	159	PF00069	0.372
MOD_PKA_2	392	398	PF00069	0.322
MOD_PKA_2	403	409	PF00069	0.348
MOD_PKA_2	71	77	PF00069	0.320
MOD_Plk_1	185	191	PF00069	0.480
MOD_Plk_1	199	205	PF00069	0.297
MOD_Plk_1	247	253	PF00069	0.362
MOD_Plk_4	159	165	PF00069	0.320
MOD_Plk_4	185	191	PF00069	0.480
MOD_Plk_4	199	205	PF00069	0.275
MOD_Plk_4	207	213	PF00069	0.250
MOD_Plk_4	34	40	PF00069	0.444
MOD_Plk_4	396	402	PF00069	0.372
MOD_Plk_4	465	471	PF00069	0.347
MOD_Plk_4	54	60	PF00069	0.368
MOD_ProDKin_1	3	9	PF00069	0.575
MOD_ProDKin_1	364	370	PF00069	0.401
MOD_ProDKin_1	435	441	PF00069	0.303
MOD_SUMO_rev_2	162	169	PF00179	0.451
MOD_SUMO_rev_2	202	211	PF00179	0.451
MOD_SUMO_rev_2	264	273	PF00179	0.401
MOD_SUMO_rev_2	512	519	PF00179	0.458
TRG_DiLeu_BaEn_2	350	356	PF01217	0.229
TRG_DiLeu_BaLyEn_6	106	111	PF01217	0.367
TRG_DiLeu_BaLyEn_6	26	31	PF01217	0.483
TRG_DiLeu_BaLyEn_6	4	9	PF01217	0.693
TRG_ENDOCYTIC_2	145	148	PF00928	0.478
TRG_ENDOCYTIC_2	187	190	PF00928	0.372
TRG_ENDOCYTIC_2	262	265	PF00928	0.386
TRG_ENDOCYTIC_2	28	31	PF00928	0.487
TRG_ER_diArg_1	2	4	PF00400	0.547
TRG_NES_CRM1_1	506	518	PF08389	0.293
TRG_Pf-PMV_PEXEL_1	219	223	PF00026	0.334
TRG_Pf-PMV_PEXEL_1	278	283	PF00026	0.408
TRG_Pf-PMV_PEXEL_1	29	34	PF00026	0.431
TRG_Pf-PMV_PEXEL_1	354	358	PF00026	0.240
TRG_Pf-PMV_PEXEL_1	80	84	PF00026	0.388

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I631	Leptomonas seymouri	60%	97%
A0A0N1PCN7	Leptomonas seymouri	85%	100%
A0A1X0NIU7	Trypanosomatidae	75%	99%
A0A1X0NZZ8	Trypanosomatidae	61%	99%
A0A3R7N408	Trypanosoma rangeli	75%	99%
A0A3S5IRI9	Trypanosoma rangeli	60%	97%
A0A3S7WY69	Leishmania donovani	92%	100%
A2CIN0	Leishmania infantum	63%	97%
A2CIN8	Leishmania donovani	63%	97%
A4HHJ6	Leishmania braziliensis	61%	97%
C9ZLE3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	60%	97%
D0A701	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	77%	99%
E9AE57	Leishmania major	63%	100%
E9AH43	Leishmania infantum	92%	100%
E9AWM1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	91%	100%
P0AC33	Escherichia coli (strain K12)	62%	100%
P0AC34	Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)	62%	100%
P14407	Escherichia coli (strain K12)	62%	100%
P40720	Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)	62%	95%
Q04718	Geobacillus stearothermophilus	24%	100%
Q4QAU9	Leishmania major	91%	100%
Q8X4P8	Escherichia coli O157:H7	62%	100%
V5BGH8	Trypanosoma cruzi	61%	97%
V5DSN1	Trypanosoma cruzi	74%	99%