Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

poly A polymerase regulatory subunit, putative

Species:

Leishmania braziliensis

UniProt:

A4HD69_LEIBR

TriTrypDb:

LbrM.24.0110 , LBRM2903_240005900

Length:

437

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	4
GO:0110165	cellular anatomical entity	1	4
GO:0031974	membrane-enclosed lumen	2	1
GO:0031981	nuclear lumen	5	1
GO:0043233	organelle lumen	3	1
GO:0070013	intracellular organelle lumen	4	1

Expansion

Sequence features

A4HD69

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HD69

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006370	7-methylguanosine mRNA capping	8	12
GO:0006396	RNA processing	6	12
GO:0006397	mRNA processing	7	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009452	7-methylguanosine RNA capping	8	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0016071	mRNA metabolic process	6	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0036260	RNA capping	7	12
GO:0043170	macromolecule metabolic process	3	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	12
GO:0003746	translation elongation factor activity	4	12
GO:0003824	catalytic activity	1	12
GO:0004483	mRNA (nucleoside-2'-O-)-methyltransferase activity	6	12
GO:0005488	binding	1	12
GO:0008135	translation factor activity, RNA binding	3	12
GO:0008168	methyltransferase activity	4	12
GO:0008171	O-methyltransferase activity	5	12
GO:0008173	RNA methyltransferase activity	4	12
GO:0008174	mRNA methyltransferase activity	5	12
GO:0008757	S-adenosylmethionine-dependent methyltransferase activity	5	12
GO:0016740	transferase activity	2	12
GO:0016741	transferase activity, transferring one-carbon groups	3	12
GO:0045182	translation regulator activity	1	12
GO:0090079	translation regulator activity, nucleic acid binding	2	12
GO:0097159	organic cyclic compound binding	2	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	215	219	PF00656	0.284
CLV_NRD_NRD_1	133	135	PF00675	0.541
CLV_NRD_NRD_1	15	17	PF00675	0.321
CLV_NRD_NRD_1	251	253	PF00675	0.533
CLV_NRD_NRD_1	39	41	PF00675	0.242
CLV_NRD_NRD_1	425	427	PF00675	0.538
CLV_NRD_NRD_1	5	7	PF00675	0.231
CLV_PCSK_FUR_1	131	135	PF00082	0.498
CLV_PCSK_KEX2_1	133	135	PF00082	0.506
CLV_PCSK_KEX2_1	15	17	PF00082	0.307
CLV_PCSK_KEX2_1	251	253	PF00082	0.534
CLV_PCSK_KEX2_1	421	423	PF00082	0.475
CLV_PCSK_KEX2_1	425	427	PF00082	0.492
CLV_PCSK_KEX2_1	431	433	PF00082	0.534
CLV_PCSK_KEX2_1	5	7	PF00082	0.278
CLV_PCSK_PC1ET2_1	421	423	PF00082	0.475
CLV_PCSK_PC1ET2_1	431	433	PF00082	0.535
CLV_PCSK_SKI1_1	279	283	PF00082	0.480
CLV_PCSK_SKI1_1	325	329	PF00082	0.501
CLV_PCSK_SKI1_1	41	45	PF00082	0.257
CLV_PCSK_SKI1_1	6	10	PF00082	0.340
DEG_Nend_Nbox_1	1	3	PF02207	0.534
DEG_SPOP_SBC_1	228	232	PF00917	0.231
DOC_CKS1_1	123	128	PF01111	0.252
DOC_CKS1_1	377	382	PF01111	0.216
DOC_CYCLIN_yCln2_LP_2	311	317	PF00134	0.282
DOC_CYCLIN_yCln2_LP_2	79	85	PF00134	0.360
DOC_CYCLIN_yCln2_LP_2	95	101	PF00134	0.172
DOC_MAPK_MEF2A_6	229	238	PF00069	0.231
DOC_PP2B_LxvP_1	79	82	PF13499	0.411
DOC_PP4_FxxP_1	123	126	PF00568	0.176
DOC_PP4_FxxP_1	189	192	PF00568	0.231
DOC_PP4_FxxP_1	27	30	PF00568	0.601
DOC_USP7_MATH_1	100	104	PF00917	0.246
DOC_USP7_MATH_1	17	21	PF00917	0.506
DOC_USP7_MATH_1	228	232	PF00917	0.238
DOC_USP7_MATH_1	292	296	PF00917	0.433
DOC_WW_Pin1_4	122	127	PF00397	0.252
DOC_WW_Pin1_4	376	381	PF00397	0.292
LIG_14-3-3_CanoR_1	131	137	PF00244	0.263
LIG_14-3-3_CanoR_1	19	27	PF00244	0.501
LIG_14-3-3_CanoR_1	325	330	PF00244	0.264
LIG_CaM_NSCaTE_8	326	333	PF13499	0.269
LIG_deltaCOP1_diTrp_1	175	178	PF00928	0.308
LIG_FHA_1	123	129	PF00498	0.238
LIG_FHA_1	137	143	PF00498	0.242
LIG_FHA_1	161	167	PF00498	0.259
LIG_FHA_1	218	224	PF00498	0.304
LIG_FHA_2	133	139	PF00498	0.231
LIG_FHA_2	207	213	PF00498	0.387
LIG_FHA_2	254	260	PF00498	0.312
LIG_FHA_2	300	306	PF00498	0.360
LIG_KLC1_Yacidic_2	213	218	PF13176	0.278
LIG_LIR_Apic_2	121	126	PF02991	0.176
LIG_LIR_Apic_2	24	30	PF02991	0.597
LIG_LIR_Gen_1	212	222	PF02991	0.335
LIG_LIR_Gen_1	390	399	PF02991	0.443
LIG_LIR_Gen_1	48	56	PF02991	0.479
LIG_LIR_Gen_1	90	101	PF02991	0.300
LIG_LIR_LC3C_4	233	238	PF02991	0.277
LIG_LIR_Nem_3	212	217	PF02991	0.335
LIG_LIR_Nem_3	291	296	PF02991	0.266
LIG_LIR_Nem_3	379	385	PF02991	0.269
LIG_LIR_Nem_3	390	394	PF02991	0.297
LIG_LIR_Nem_3	48	53	PF02991	0.479
LIG_LIR_Nem_3	86	91	PF02991	0.386
LIG_LIR_Nem_3	92	96	PF02991	0.283
LIG_PCNA_TLS_4	279	286	PF02747	0.364
LIG_Pex14_2	382	386	PF04695	0.388
LIG_SH2_CRK	395	399	PF00017	0.281
LIG_SH2_NCK_1	246	250	PF00017	0.425
LIG_SH2_SRC	216	219	PF00017	0.305
LIG_SH2_STAP1	263	267	PF00017	0.260
LIG_SH2_STAT5	136	139	PF00017	0.204
LIG_SH2_STAT5	214	217	PF00017	0.260
LIG_SH2_STAT5	285	288	PF00017	0.277
LIG_SH2_STAT5	341	344	PF00017	0.419
LIG_SH2_STAT5	395	398	PF00017	0.411
LIG_SH3_1	188	194	PF00018	0.231
LIG_SH3_2	14	19	PF14604	0.458
LIG_SH3_3	11	17	PF00018	0.621
LIG_SH3_3	188	194	PF00018	0.234
LIG_SH3_3	222	228	PF00018	0.243
LIG_SH3_3	235	241	PF00018	0.257
LIG_SH3_3	311	317	PF00018	0.288
LIG_SH3_3	374	380	PF00018	0.230
LIG_SUMO_SIM_par_1	114	122	PF11976	0.245
LIG_TRAF2_1	210	213	PF00917	0.278
LIG_TRAF2_1	257	260	PF00917	0.351
LIG_TRFH_1	85	89	PF08558	0.411
LIG_TYR_ITIM	339	344	PF00017	0.480
MOD_CK1_1	199	205	PF00069	0.302
MOD_CK1_1	20	26	PF00069	0.434
MOD_CK1_1	62	68	PF00069	0.272
MOD_CK2_1	132	138	PF00069	0.411
MOD_CK2_1	206	212	PF00069	0.399
MOD_CK2_1	227	233	PF00069	0.190
MOD_CK2_1	253	259	PF00069	0.374
MOD_CK2_1	299	305	PF00069	0.424
MOD_CK2_1	42	48	PF00069	0.266
MOD_Cter_Amidation	428	431	PF01082	0.767
MOD_GlcNHglycan	149	152	PF01048	0.266
MOD_GlcNHglycan	286	289	PF01048	0.427
MOD_GlcNHglycan	336	339	PF01048	0.276
MOD_GlcNHglycan	61	64	PF01048	0.378
MOD_GSK3_1	107	114	PF00069	0.326
MOD_GSK3_1	132	139	PF00069	0.325
MOD_GSK3_1	143	150	PF00069	0.258
MOD_GSK3_1	17	24	PF00069	0.343
MOD_GSK3_1	196	203	PF00069	0.353
MOD_GSK3_1	297	304	PF00069	0.326
MOD_GSK3_1	325	332	PF00069	0.349
MOD_GSK3_1	54	61	PF00069	0.299
MOD_GSK3_1	83	90	PF00069	0.189
MOD_LATS_1	323	329	PF00433	0.441
MOD_N-GLC_1	20	25	PF02516	0.442
MOD_N-GLC_1	58	63	PF02516	0.208
MOD_NEK2_1	217	222	PF00069	0.411
MOD_NEK2_1	299	304	PF00069	0.381
MOD_NEK2_1	329	334	PF00069	0.346
MOD_NEK2_1	83	88	PF00069	0.412
MOD_PIKK_1	100	106	PF00454	0.364
MOD_PIKK_1	217	223	PF00454	0.331
MOD_PKA_1	430	436	PF00069	0.673
MOD_PKA_2	132	138	PF00069	0.307
MOD_PKA_2	411	417	PF00069	0.620
MOD_Plk_1	207	213	PF00069	0.331
MOD_Plk_1	347	353	PF00069	0.396
MOD_Plk_2-3	212	218	PF00069	0.331
MOD_Plk_4	212	218	PF00069	0.331
MOD_Plk_4	281	287	PF00069	0.378
MOD_Plk_4	325	331	PF00069	0.350
MOD_ProDKin_1	122	128	PF00069	0.295
MOD_ProDKin_1	376	382	PF00069	0.343
MOD_SUMO_rev_2	365	373	PF00179	0.303
TRG_DiLeu_BaEn_2	45	51	PF01217	0.295
TRG_DiLeu_BaEn_4	212	218	PF01217	0.331
TRG_DiLeu_BaLyEn_6	27	32	PF01217	0.496
TRG_DiLeu_BaLyEn_6	276	281	PF01217	0.372
TRG_ENDOCYTIC_2	214	217	PF00928	0.411
TRG_ENDOCYTIC_2	293	296	PF00928	0.387
TRG_ENDOCYTIC_2	309	312	PF00928	0.517
TRG_ENDOCYTIC_2	341	344	PF00928	0.508
TRG_ER_diArg_1	130	133	PF00400	0.421
TRG_ER_diArg_1	14	16	PF00400	0.362
TRG_ER_diArg_1	251	253	PF00400	0.445
TRG_ER_diArg_1	409	412	PF00400	0.460
TRG_ER_diArg_1	425	427	PF00400	0.757
TRG_ER_diArg_1	5	7	PF00400	0.265
TRG_Pf-PMV_PEXEL_1	279	283	PF00026	0.450
TRG_Pf-PMV_PEXEL_1	301	305	PF00026	0.395

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HV56	Leptomonas seymouri	67%	100%
A0A0S4J219	Bodo saltans	49%	100%
A0A1X0NIX1	Trypanosomatidae	47%	100%
A0A3S7WY48	Leishmania donovani	81%	100%
A0A422P442	Trypanosoma rangeli	51%	100%
D0A6Y2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	51%	100%
E9AH23	Leishmania infantum	81%	100%
E9AWK1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	83%	100%
Q4QAW9	Leishmania major	82%	100%
V5BRC4	Trypanosoma cruzi	49%	100%