Putative clathrin coat assembly protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

clathrin coat assembly protein, putative

Species:

Leishmania braziliensis

UniProt:

A4HD60_LEIBR

TriTrypDb:

LbrM.24.0020 , LBRM2903_240005100

Length:

486

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0030135	coated vesicle	7	12
GO:0030136	clathrin-coated vesicle	8	12
GO:0031410	cytoplasmic vesicle	6	12
GO:0031982	vesicle	4	12
GO:0043226	organelle	2	12
GO:0043227	membrane-bounded organelle	3	12
GO:0043229	intracellular organelle	3	12
GO:0043231	intracellular membrane-bounded organelle	4	12
GO:0097708	intracellular vesicle	5	12
GO:0110165	cellular anatomical entity	1	12
GO:0000331	contractile vacuole	6	1
GO:0005737	cytoplasm	2	1
GO:0005773	vacuole	5	1
GO:0005905	clathrin-coated pit	4	1
GO:0016020	membrane	2	1
GO:0098590	plasma membrane region	3	1

Expansion

Sequence features

A4HD60

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HD60

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	12
GO:0006897	endocytosis	5	12
GO:0006898	receptor-mediated endocytosis	6	12
GO:0009987	cellular process	1	12
GO:0016043	cellular component organization	3	12
GO:0016192	vesicle-mediated transport	4	12
GO:0022607	cellular component assembly	4	12
GO:0043933	protein-containing complex organization	4	12
GO:0048268	clathrin coat assembly	6	12
GO:0051179	localization	1	12
GO:0051234	establishment of localization	2	12
GO:0065003	protein-containing complex assembly	5	12
GO:0071840	cellular component organization or biogenesis	2	12
GO:0072583	clathrin-dependent endocytosis	7	12
GO:0006900	vesicle budding from membrane	5	1
GO:0006996	organelle organization	4	1
GO:0016050	vesicle organization	5	1
GO:0061024	membrane organization	4	1

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	12
GO:0005515	protein binding	2	12
GO:0005543	phospholipid binding	3	12
GO:0005545	1-phosphatidylinositol binding	5	12
GO:0008289	lipid binding	2	12
GO:0030276	clathrin binding	3	12
GO:0035091	phosphatidylinositol binding	4	12
GO:0000149	SNARE binding	3	1
GO:0005546	phosphatidylinositol-4,5-bisphosphate binding	4	1
GO:0032050	clathrin heavy chain binding	4	1
GO:0043167	ion binding	2	1
GO:0043168	anion binding	3	1
GO:1901981	phosphatidylinositol phosphate binding	5	1
GO:1902936	phosphatidylinositol bisphosphate binding	6	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	103	107	PF00656	0.401
CLV_C14_Caspase3-7	129	133	PF00656	0.343
CLV_C14_Caspase3-7	344	348	PF00656	0.717
CLV_NRD_NRD_1	161	163	PF00675	0.383
CLV_NRD_NRD_1	247	249	PF00675	0.352
CLV_NRD_NRD_1	254	256	PF00675	0.322
CLV_PCSK_FUR_1	159	163	PF00082	0.165
CLV_PCSK_KEX2_1	120	122	PF00082	0.406
CLV_PCSK_KEX2_1	161	163	PF00082	0.370
CLV_PCSK_KEX2_1	221	223	PF00082	0.305
CLV_PCSK_KEX2_1	229	231	PF00082	0.369
CLV_PCSK_KEX2_1	246	248	PF00082	0.243
CLV_PCSK_KEX2_1	254	256	PF00082	0.318
CLV_PCSK_KEX2_1	80	82	PF00082	0.243
CLV_PCSK_PC1ET2_1	120	122	PF00082	0.406
CLV_PCSK_PC1ET2_1	221	223	PF00082	0.305
CLV_PCSK_PC1ET2_1	229	231	PF00082	0.369
CLV_PCSK_PC1ET2_1	246	248	PF00082	0.388
CLV_PCSK_PC1ET2_1	80	82	PF00082	0.243
CLV_PCSK_PC7_1	76	82	PF00082	0.388
CLV_PCSK_SKI1_1	116	120	PF00082	0.326
CLV_PCSK_SKI1_1	221	225	PF00082	0.296
CLV_PCSK_SKI1_1	230	234	PF00082	0.217
CLV_PCSK_SKI1_1	31	35	PF00082	0.388
DEG_Nend_UBRbox_3	1	3	PF02207	0.511
DOC_MAPK_DCC_7	189	199	PF00069	0.165
DOC_MAPK_gen_1	14	21	PF00069	0.373
DOC_MAPK_gen_1	246	252	PF00069	0.249
DOC_PP1_RVXF_1	227	234	PF00149	0.280
DOC_PP2B_LxvP_1	41	44	PF13499	0.258
DOC_PP2B_LxvP_1	463	466	PF13499	0.463
DOC_PP4_FxxP_1	241	244	PF00568	0.412
DOC_PP4_FxxP_1	277	280	PF00568	0.277
DOC_USP7_MATH_1	104	108	PF00917	0.242
DOC_USP7_MATH_1	259	263	PF00917	0.446
DOC_USP7_MATH_1	335	339	PF00917	0.633
DOC_USP7_MATH_1	390	394	PF00917	0.679
DOC_USP7_MATH_1	464	468	PF00917	0.616
DOC_USP7_MATH_1	6	10	PF00917	0.448
DOC_USP7_UBL2_3	116	120	PF12436	0.249
DOC_USP7_UBL2_3	31	35	PF12436	0.388
DOC_USP7_UBL2_3	47	51	PF12436	0.388
DOC_WW_Pin1_4	350	355	PF00397	0.675
DOC_WW_Pin1_4	458	463	PF00397	0.658
LIG_14-3-3_CanoR_1	254	263	PF00244	0.337
LIG_14-3-3_CanoR_1	81	90	PF00244	0.260
LIG_AP2alpha_2	472	474	PF02296	0.545
LIG_APCC_ABBA_1	471	476	PF00400	0.510
LIG_BRCT_BRCA1_1	273	277	PF00533	0.337
LIG_FHA_1	16	22	PF00498	0.534
LIG_FHA_1	172	178	PF00498	0.360
LIG_FHA_1	35	41	PF00498	0.131
LIG_FHA_1	424	430	PF00498	0.610
LIG_FHA_2	303	309	PF00498	0.548
LIG_FHA_2	325	331	PF00498	0.621
LIG_LIR_Apic_2	239	244	PF02991	0.412
LIG_LIR_Apic_2	274	280	PF02991	0.337
LIG_LIR_Gen_1	167	175	PF02991	0.367
LIG_LIR_Gen_1	181	191	PF02991	0.305
LIG_LIR_Gen_1	211	217	PF02991	0.261
LIG_LIR_Gen_1	236	244	PF02991	0.313
LIG_LIR_Gen_1	258	267	PF02991	0.311
LIG_LIR_Gen_1	84	95	PF02991	0.243
LIG_LIR_Gen_1	96	104	PF02991	0.243
LIG_LIR_Nem_3	141	146	PF02991	0.307
LIG_LIR_Nem_3	167	171	PF02991	0.322
LIG_LIR_Nem_3	181	187	PF02991	0.306
LIG_LIR_Nem_3	211	216	PF02991	0.252
LIG_LIR_Nem_3	236	241	PF02991	0.295
LIG_LIR_Nem_3	251	256	PF02991	0.342
LIG_LIR_Nem_3	258	263	PF02991	0.270
LIG_LIR_Nem_3	472	477	PF02991	0.469
LIG_LIR_Nem_3	56	60	PF02991	0.260
LIG_LIR_Nem_3	84	90	PF02991	0.243
LIG_LIR_Nem_3	96	100	PF02991	0.243
LIG_MYND_1	282	286	PF01753	0.277
LIG_NRBOX	186	192	PF00104	0.291
LIG_PCNA_yPIPBox_3	27	35	PF02747	0.165
LIG_Pex14_1	249	253	PF04695	0.243
LIG_Rb_LxCxE_1	313	330	PF01857	0.412
LIG_SH2_CRK	115	119	PF00017	0.307
LIG_SH2_CRK	213	217	PF00017	0.251
LIG_SH2_NCK_1	256	260	PF00017	0.302
LIG_SH2_STAP1	256	260	PF00017	0.344
LIG_SH2_STAT5	12	15	PF00017	0.384
LIG_SH2_STAT5	146	149	PF00017	0.254
LIG_SH2_STAT5	266	269	PF00017	0.443
LIG_SH2_STAT5	48	51	PF00017	0.271
LIG_SH3_1	368	374	PF00018	0.598
LIG_SH3_2	470	475	PF14604	0.545
LIG_SH3_3	351	357	PF00018	0.541
LIG_SH3_3	368	374	PF00018	0.556
LIG_SH3_3	379	385	PF00018	0.585
LIG_SH3_3	467	473	PF00018	0.587
LIG_SUMO_SIM_par_1	87	93	PF11976	0.258
LIG_TRAF2_1	327	330	PF00917	0.501
LIG_TRAF2_1	367	370	PF00917	0.560
LIG_UBA3_1	223	229	PF00899	0.388
LIG_WRC_WIRS_1	21	26	PF05994	0.435
LIG_WRC_WIRS_1	403	408	PF05994	0.459
LIG_WRC_WIRS_1	94	99	PF05994	0.306
MOD_CK1_1	22	28	PF00069	0.503
MOD_CK1_1	302	308	PF00069	0.561
MOD_CK1_1	383	389	PF00069	0.707
MOD_CK1_1	458	464	PF00069	0.581
MOD_CK2_1	104	110	PF00069	0.463
MOD_CK2_1	239	245	PF00069	0.380
MOD_CK2_1	302	308	PF00069	0.577
MOD_CK2_1	324	330	PF00069	0.657
MOD_CK2_1	364	370	PF00069	0.451
MOD_Cter_Amidation	159	162	PF01082	0.388
MOD_Cter_Amidation	219	222	PF01082	0.217
MOD_GlcNHglycan	129	132	PF01048	0.241
MOD_GlcNHglycan	154	157	PF01048	0.308
MOD_GlcNHglycan	24	27	PF01048	0.521
MOD_GlcNHglycan	257	260	PF01048	0.334
MOD_GlcNHglycan	386	389	PF01048	0.633
MOD_GlcNHglycan	438	441	PF01048	0.658
MOD_GlcNHglycan	8	11	PF01048	0.552
MOD_GSK3_1	104	111	PF00069	0.346
MOD_GSK3_1	15	22	PF00069	0.413
MOD_GSK3_1	255	262	PF00069	0.319
MOD_GSK3_1	266	273	PF00069	0.296
MOD_GSK3_1	376	383	PF00069	0.639
MOD_GSK3_1	418	425	PF00069	0.629
MOD_N-GLC_1	1	6	PF02516	0.593
MOD_N-GLC_1	409	414	PF02516	0.476
MOD_N-GLC_1	447	452	PF02516	0.524
MOD_N-GLC_2	206	208	PF02516	0.258
MOD_NEK2_1	108	113	PF00069	0.360
MOD_NEK2_1	119	124	PF00069	0.316
MOD_NEK2_1	138	143	PF00069	0.423
MOD_NEK2_1	171	176	PF00069	0.430
MOD_NEK2_1	271	276	PF00069	0.334
MOD_NEK2_1	34	39	PF00069	0.275
MOD_NEK2_1	364	369	PF00069	0.424
MOD_NEK2_1	427	432	PF00069	0.672
MOD_NEK2_2	114	119	PF00069	0.165
MOD_NEK2_2	259	264	PF00069	0.388
MOD_PIKK_1	138	144	PF00454	0.274
MOD_PKA_2	299	305	PF00069	0.405
MOD_Plk_1	239	245	PF00069	0.284
MOD_Plk_1	478	484	PF00069	0.395
MOD_Plk_2-3	239	245	PF00069	0.285
MOD_Plk_2-3	308	314	PF00069	0.504
MOD_Plk_4	104	110	PF00069	0.258
MOD_Plk_4	114	120	PF00069	0.182
MOD_Plk_4	266	272	PF00069	0.329
MOD_Plk_4	287	293	PF00069	0.277
MOD_Plk_4	90	96	PF00069	0.258
MOD_ProDKin_1	350	356	PF00069	0.671
MOD_ProDKin_1	458	464	PF00069	0.656
MOD_SUMO_rev_2	326	333	PF00179	0.652
TRG_DiLeu_BaEn_1	211	216	PF01217	0.388
TRG_DiLeu_BaEn_2	166	172	PF01217	0.388
TRG_DiLeu_BaLyEn_6	186	191	PF01217	0.279
TRG_DiLeu_BaLyEn_6	96	101	PF01217	0.307
TRG_ENDOCYTIC_2	115	118	PF00928	0.388
TRG_ENDOCYTIC_2	213	216	PF00928	0.301
TRG_ER_diArg_1	121	124	PF00400	0.165
TRG_ER_diArg_1	159	162	PF00400	0.416
TRG_ER_diArg_1	247	249	PF00400	0.325
TRG_ER_diArg_1	253	255	PF00400	0.284
TRG_NES_CRM1_1	178	192	PF08389	0.192
TRG_Pf-PMV_PEXEL_1	99	103	PF00026	0.307

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1II26	Leptomonas seymouri	59%	100%
A0A0S4J0L1	Bodo saltans	35%	100%
A0A1X0NIY0	Trypanosomatidae	40%	97%
A0A3S5H7C6	Leishmania donovani	72%	100%
A0A422P449	Trypanosoma rangeli	42%	97%
A4I0M5	Leishmania infantum	72%	100%
D0A6X3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	40%	99%
E9AWJ2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	72%	100%
P94017	Arabidopsis thaliana	24%	70%
Q4QAX8	Leishmania major	70%	98%
Q8LBH2	Arabidopsis thaliana	25%	85%
Q9LHS0	Arabidopsis thaliana	23%	89%
V5DSQ8	Trypanosoma cruzi	42%	97%