Sphingolipid 4-desaturase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

fatty acid desaturase

Species:

Leishmania braziliensis

UniProt:

A4HD58_LEIBR

TriTrypDb:

LbrM.23.1980 , LBRM2903_230031100

Length:

344

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 18

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 15
NetGPI	no	yes: 0, no: 15

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	16
GO:0110165	cellular anatomical entity	1	16

Expansion

Sequence features

A4HD58

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HD58

Function

Biological processes
Term	Name	Level	Count
GO:0006629	lipid metabolic process	3	16
GO:0006643	membrane lipid metabolic process	4	14
GO:0006665	sphingolipid metabolic process	4	14
GO:0006807	nitrogen compound metabolic process	2	14
GO:0008152	metabolic process	1	16
GO:0008610	lipid biosynthetic process	4	14
GO:0009058	biosynthetic process	2	14
GO:0009987	cellular process	1	14
GO:0030148	sphingolipid biosynthetic process	5	14
GO:0044237	cellular metabolic process	2	14
GO:0044238	primary metabolic process	2	16
GO:0044249	cellular biosynthetic process	3	14
GO:0044255	cellular lipid metabolic process	3	14
GO:0046467	membrane lipid biosynthetic process	4	14
GO:0071704	organic substance metabolic process	2	16
GO:1901564	organonitrogen compound metabolic process	3	14
GO:1901566	organonitrogen compound biosynthetic process	4	14
GO:1901576	organic substance biosynthetic process	3	14
GO:0006672	ceramide metabolic process	4	2
GO:0034641	cellular nitrogen compound metabolic process	3	2
GO:0043603	amide metabolic process	3	2
GO:0043604	amide biosynthetic process	4	2
GO:0044271	cellular nitrogen compound biosynthetic process	4	2
GO:0046513	ceramide biosynthetic process	5	2

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	14
GO:0016491	oxidoreductase activity	2	14
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen	3	14
GO:0016717	oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water	4	14
GO:0042284	sphingolipid delta-4 desaturase activity	5	14

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	277	279	PF00675	0.238
CLV_PCSK_KEX2_1	321	323	PF00082	0.304
CLV_PCSK_PC1ET2_1	321	323	PF00082	0.318
CLV_PCSK_PC7_1	317	323	PF00082	0.343
CLV_PCSK_SKI1_1	158	162	PF00082	0.307
CLV_PCSK_SKI1_1	177	181	PF00082	0.209
CLV_PCSK_SKI1_1	24	28	PF00082	0.168
CLV_PCSK_SKI1_1	46	50	PF00082	0.275
DOC_CKS1_1	47	52	PF01111	0.341
DOC_CKS1_1	98	103	PF01111	0.500
DOC_CYCLIN_RxL_1	155	163	PF00134	0.288
DOC_CYCLIN_yCln2_LP_2	113	119	PF00134	0.313
DOC_MAPK_gen_1	177	186	PF00069	0.476
DOC_MAPK_MEF2A_6	46	54	PF00069	0.308
DOC_MAPK_MEF2A_6	63	72	PF00069	0.333
DOC_PP1_RVXF_1	130	137	PF00149	0.291
DOC_PP1_RVXF_1	175	182	PF00149	0.475
DOC_PP2B_LxvP_1	113	116	PF13499	0.313
DOC_PP4_FxxP_1	98	101	PF00568	0.510
DOC_WW_Pin1_4	46	51	PF00397	0.341
DOC_WW_Pin1_4	97	102	PF00397	0.500
LIG_14-3-3_CanoR_1	273	280	PF00244	0.543
LIG_14-3-3_CanoR_1	290	295	PF00244	0.544
LIG_AP2alpha_2	41	43	PF02296	0.476
LIG_BIR_II_1	1	5	PF00653	0.583
LIG_BRCT_BRCA1_1	229	233	PF00533	0.523
LIG_CSL_BTD_1	42	45	PF09270	0.383
LIG_eIF4E_1	155	161	PF01652	0.288
LIG_FHA_1	161	167	PF00498	0.307
LIG_FHA_1	192	198	PF00498	0.299
LIG_FHA_1	47	53	PF00498	0.320
LIG_FHA_1	67	73	PF00498	0.329
LIG_FHA_1	91	97	PF00498	0.451
LIG_FHA_2	143	149	PF00498	0.263
LIG_FHA_2	295	301	PF00498	0.468
LIG_FXI_DFP_1	263	267	PF00024	0.242
LIG_HP1_1	50	54	PF01393	0.156
LIG_KLC1_Yacidic_2	283	287	PF13176	0.533
LIG_LIR_Apic_2	243	249	PF02991	0.442
LIG_LIR_Gen_1	100	111	PF02991	0.462
LIG_LIR_Gen_1	163	171	PF02991	0.307
LIG_LIR_Gen_1	178	189	PF02991	0.451
LIG_LIR_Gen_1	194	202	PF02991	0.254
LIG_LIR_Gen_1	230	240	PF02991	0.344
LIG_LIR_Gen_1	297	307	PF02991	0.553
LIG_LIR_Nem_3	100	106	PF02991	0.460
LIG_LIR_Nem_3	163	167	PF02991	0.300
LIG_LIR_Nem_3	194	198	PF02991	0.248
LIG_LIR_Nem_3	219	224	PF02991	0.341
LIG_LIR_Nem_3	230	236	PF02991	0.441
LIG_LIR_Nem_3	242	247	PF02991	0.442
LIG_LIR_Nem_3	265	269	PF02991	0.442
LIG_LIR_Nem_3	283	288	PF02991	0.489
LIG_LIR_Nem_3	297	302	PF02991	0.468
LIG_LIR_Nem_3	64	70	PF02991	0.237
LIG_Pex14_1	206	210	PF04695	0.291
LIG_Pex14_1	255	259	PF04695	0.476
LIG_Pex14_2	164	168	PF04695	0.304
LIG_Pex14_2	202	206	PF04695	0.390
LIG_Pex14_2	212	216	PF04695	0.402
LIG_Pex14_2	98	102	PF04695	0.372
LIG_PTB_Apo_2	101	108	PF02174	0.476
LIG_PTB_Apo_2	253	260	PF02174	0.533
LIG_PTB_Phospho_1	101	107	PF10480	0.491
LIG_PTB_Phospho_1	253	259	PF10480	0.533
LIG_SH2_CRK	119	123	PF00017	0.156
LIG_SH2_CRK	247	251	PF00017	0.442
LIG_SH2_CRK	310	314	PF00017	0.410
LIG_SH2_NCK_1	119	123	PF00017	0.156
LIG_SH2_PTP2	285	288	PF00017	0.581
LIG_SH2_STAP1	107	111	PF00017	0.333
LIG_SH2_STAP1	119	123	PF00017	0.300
LIG_SH2_STAP1	310	314	PF00017	0.489
LIG_SH2_STAT3	189	192	PF00017	0.341
LIG_SH2_STAT5	133	136	PF00017	0.242
LIG_SH2_STAT5	151	154	PF00017	0.242
LIG_SH2_STAT5	189	192	PF00017	0.333
LIG_SH2_STAT5	201	204	PF00017	0.269
LIG_SH2_STAT5	210	213	PF00017	0.265
LIG_SH2_STAT5	285	288	PF00017	0.578
LIG_SH2_STAT5	57	60	PF00017	0.331
LIG_SH2_STAT5	75	78	PF00017	0.320
LIG_SH3_3	112	118	PF00018	0.323
LIG_SH3_3	264	270	PF00018	0.450
LIG_SH3_3	39	45	PF00018	0.383
LIG_SUMO_SIM_anti_2	49	55	PF11976	0.156
LIG_SUMO_SIM_anti_2	69	74	PF11976	0.441
LIG_TRFH_1	301	305	PF08558	0.568
LIG_TYR_ITIM	117	122	PF00017	0.156
LIG_TYR_ITIM	208	213	PF00017	0.363
LIG_WRC_WIRS_1	161	166	PF05994	0.307
MOD_CK2_1	10	16	PF00069	0.396
MOD_CK2_1	142	148	PF00069	0.263
MOD_CMANNOS	203	206	PF00535	0.441
MOD_CMANNOS	252	255	PF00535	0.263
MOD_GlcNHglycan	1	4	PF01048	0.443
MOD_GSK3_1	138	145	PF00069	0.308
MOD_GSK3_1	171	178	PF00069	0.533
MOD_GSK3_1	290	297	PF00069	0.456
MOD_N-GLC_2	95	97	PF02516	0.238
MOD_NEK2_1	160	165	PF00069	0.321
MOD_NEK2_1	191	196	PF00069	0.242
MOD_NEK2_1	211	216	PF00069	0.289
MOD_NEK2_1	217	222	PF00069	0.298
MOD_NEK2_1	288	293	PF00069	0.581
MOD_PIKK_1	142	148	PF00454	0.144
MOD_PIKK_1	272	278	PF00454	0.344
MOD_PKA_2	171	177	PF00069	0.476
MOD_PKA_2	272	278	PF00069	0.438
MOD_Plk_4	171	177	PF00069	0.491
MOD_Plk_4	211	217	PF00069	0.331
MOD_Plk_4	294	300	PF00069	0.437
MOD_Plk_4	66	72	PF00069	0.268
MOD_ProDKin_1	46	52	PF00069	0.341
MOD_ProDKin_1	97	103	PF00069	0.500
MOD_SUMO_for_1	179	182	PF00179	0.533
MOD_SUMO_for_1	320	323	PF00179	0.510
TRG_DiLeu_BaEn_2	230	236	PF01217	0.438
TRG_DiLeu_BaLyEn_6	204	209	PF01217	0.363
TRG_ENDOCYTIC_2	103	106	PF00928	0.481
TRG_ENDOCYTIC_2	107	110	PF00928	0.332
TRG_ENDOCYTIC_2	119	122	PF00928	0.256
TRG_ENDOCYTIC_2	210	213	PF00928	0.375
TRG_ENDOCYTIC_2	247	250	PF00928	0.442
TRG_ENDOCYTIC_2	285	288	PF00928	0.490
TRG_ENDOCYTIC_2	310	313	PF00928	0.408
TRG_ER_diArg_1	21	24	PF00400	0.362
TRG_Pf-PMV_PEXEL_1	132	137	PF00026	0.491

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P225	Leptomonas seymouri	82%	97%
A0A0S4IWE2	Bodo saltans	52%	100%
A0A0S4IZS1	Bodo saltans	50%	100%
A0A1X0NG21	Trypanosomatidae	68%	99%
A0A1X0P7H0	Trypanosomatidae	68%	99%
A0A3S5H7G4	Leishmania donovani	85%	100%
A0A3S7WZX6	Leishmania donovani	85%	100%
A4I2A2	Leishmania infantum	84%	95%
C4R613	Komagataella phaffii (strain GS115 / ATCC 20864)	41%	96%
C9ZQX1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	56%	99%
E8NHH2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	85%	100%
E9AYE9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	85%	100%
G5EC63	Caenorhabditis elegans	43%	95%
O09005	Mus musculus	42%	100%
O15121	Homo sapiens	44%	100%
O44186	Caenorhabditis elegans	42%	96%
O59715	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	46%	95%
Q0II71	Bos taurus	44%	100%
Q3ZBY7	Bos taurus	43%	100%
Q4Q923	Leishmania major	85%	100%
Q4Q925	Leishmania major	85%	100%
Q564G3	Rattus norvegicus	42%	100%
Q5AJX2	Candida albicans (strain SC5314 / ATCC MYA-2876)	43%	93%
Q5F3C1	Gallus gallus	40%	100%
Q5RE51	Pongo abelii	44%	100%
Q5XIF5	Rattus norvegicus	43%	100%
Q68FB8	Xenopus tropicalis	41%	100%
Q6H5U3	Oryza sativa subsp. japonica	45%	100%
Q6QHC5	Homo sapiens	44%	100%
Q6UQ04	Microtus fortis	41%	100%
Q8R2F2	Mus musculus	42%	100%
Q94515	Drosophila melanogaster	43%	100%
Q9ZPH4	Arabidopsis thaliana	43%	100%
V5DBA3	Trypanosoma cruzi	66%	99%