V-SNARE domain-containing protein

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein, conserved

Species:

Leishmania braziliensis

UniProt:

A4HD25_LEIBR

TriTrypDb:

LbrM.23.1650 , LBRM2903_230027300

Length:

283

Annotations

LeishMANIAdb annotations

Similar to other Eukaryotic vesicle-associated V-SNARE proteins, especially VTI1.. Localization: Vesicular (by homology)

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 1, no: 19
NetGPI	no	yes: 0, no: 20

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	16
GO:0016020	membrane	2	21
GO:0043226	organelle	2	12
GO:0043227	membrane-bounded organelle	3	12
GO:0043229	intracellular organelle	3	12
GO:0043231	intracellular membrane-bounded organelle	4	12
GO:0110165	cellular anatomical entity	1	21
GO:0005794	Golgi apparatus	5	5
GO:0005789	endoplasmic reticulum membrane	4	2
GO:0010008	endosome membrane	5	2
GO:0012506	vesicle membrane	4	2
GO:0012507	ER to Golgi transport vesicle membrane	6	2
GO:0030658	transport vesicle membrane	5	2
GO:0030659	cytoplasmic vesicle membrane	5	2
GO:0030662	coated vesicle membrane	5	2
GO:0031090	organelle membrane	3	2
GO:0031201	SNARE complex	3	2
GO:0031902	late endosome membrane	6	2
GO:0032991	protein-containing complex	1	2
GO:0098588	bounding membrane of organelle	4	2
GO:0098796	membrane protein complex	2	2

Expansion

Sequence features

A4HD25

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HD25

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	20
GO:0006886	intracellular protein transport	4	15
GO:0008104	protein localization	4	20
GO:0009987	cellular process	1	20
GO:0015031	protein transport	4	20
GO:0016192	vesicle-mediated transport	4	12
GO:0033036	macromolecule localization	2	20
GO:0045184	establishment of protein localization	3	20
GO:0046907	intracellular transport	3	15
GO:0051179	localization	1	20
GO:0051234	establishment of localization	2	20
GO:0051641	cellular localization	2	20
GO:0051649	establishment of localization in cell	3	15
GO:0070727	cellular macromolecule localization	3	20
GO:0071702	organic substance transport	4	20
GO:0071705	nitrogen compound transport	4	20
GO:0006890	retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum	6	2
GO:0048193	Golgi vesicle transport	5	2
GO:0006906	vesicle fusion	6	2
GO:0006996	organelle organization	4	2
GO:0016043	cellular component organization	3	2
GO:0016050	vesicle organization	5	2
GO:0048284	organelle fusion	5	2
GO:0061024	membrane organization	4	2
GO:0061025	membrane fusion	5	2
GO:0071840	cellular component organization or biogenesis	2	2
GO:0090174	organelle membrane fusion	6	2

Molecular functions
Term	Name	Level	Count
GO:0005484	SNAP receptor activity	3	7
GO:0030674	protein-macromolecule adaptor activity	2	7
GO:0060090	molecular adaptor activity	1	7
GO:0000149	SNARE binding	3	2
GO:0005488	binding	1	2
GO:0005515	protein binding	2	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	11	15	PF00656	0.610
CLV_C14_Caspase3-7	150	154	PF00656	0.584
CLV_C14_Caspase3-7	206	210	PF00656	0.534
CLV_NRD_NRD_1	123	125	PF00675	0.517
CLV_NRD_NRD_1	210	212	PF00675	0.230
CLV_NRD_NRD_1	251	253	PF00675	0.520
CLV_NRD_NRD_1	278	280	PF00675	0.524
CLV_NRD_NRD_1	32	34	PF00675	0.314
CLV_PCSK_KEX2_1	251	253	PF00082	0.520
CLV_PCSK_KEX2_1	32	34	PF00082	0.290
CLV_PCSK_SKI1_1	225	229	PF00082	0.335
DEG_APCC_DBOX_1	12	20	PF00400	0.525
DEG_APCC_DBOX_1	91	99	PF00400	0.504
DEG_SPOP_SBC_1	255	259	PF00917	0.494
DOC_USP7_MATH_1	107	111	PF00917	0.621
DOC_USP7_MATH_1	134	138	PF00917	0.647
DOC_USP7_MATH_1	182	186	PF00917	0.446
DOC_USP7_MATH_1	266	270	PF00917	0.533
DOC_USP7_UBL2_3	219	223	PF12436	0.608
DOC_WW_Pin1_4	251	256	PF00397	0.367
LIG_14-3-3_CanoR_1	148	152	PF00244	0.530
LIG_14-3-3_CanoR_1	187	196	PF00244	0.457
LIG_14-3-3_CanoR_1	81	89	PF00244	0.524
LIG_Actin_WH2_2	12	29	PF00022	0.553
LIG_FHA_1	173	179	PF00498	0.446
LIG_FHA_1	198	204	PF00498	0.486
LIG_FHA_1	227	233	PF00498	0.400
LIG_FHA_1	54	60	PF00498	0.479
LIG_FHA_2	148	154	PF00498	0.556
LIG_FHA_2	256	262	PF00498	0.478
LIG_FHA_2	5	11	PF00498	0.542
LIG_FHA_2	81	87	PF00498	0.505
LIG_FHA_2	95	101	PF00498	0.398
LIG_LIR_Nem_3	2	8	PF02991	0.556
LIG_SH2_CRK	37	41	PF00017	0.473
LIG_SH2_STAP1	8	12	PF00017	0.532
LIG_SH2_STAT5	244	247	PF00017	0.406
LIG_SUMO_SIM_anti_2	113	119	PF11976	0.590
LIG_SUMO_SIM_anti_2	229	235	PF11976	0.445
LIG_SUMO_SIM_par_1	198	206	PF11976	0.483
LIG_TRAF2_1	7	10	PF00917	0.557
LIG_TRAF2_1	97	100	PF00917	0.526
LIG_UBA3_1	102	106	PF00899	0.546
LIG_UBA3_1	213	219	PF00899	0.534
MOD_CK1_1	254	260	PF00069	0.548
MOD_CK1_1	275	281	PF00069	0.473
MOD_CK2_1	255	261	PF00069	0.549
MOD_CK2_1	4	10	PF00069	0.524
MOD_CK2_1	80	86	PF00069	0.472
MOD_CK2_1	94	100	PF00069	0.399
MOD_GlcNHglycan	108	112	PF01048	0.392
MOD_GlcNHglycan	214	217	PF01048	0.246
MOD_GlcNHglycan	246	249	PF01048	0.514
MOD_GSK3_1	251	258	PF00069	0.480
MOD_GSK3_1	266	273	PF00069	0.345
MOD_GSK3_1	40	47	PF00069	0.460
MOD_GSK3_1	90	97	PF00069	0.493
MOD_N-GLC_1	172	177	PF02516	0.255
MOD_NEK2_1	152	157	PF00069	0.565
MOD_NEK2_1	226	231	PF00069	0.350
MOD_NEK2_2	182	187	PF00069	0.563
MOD_PKA_2	147	153	PF00069	0.566
MOD_PKA_2	26	32	PF00069	0.442
MOD_PKA_2	80	86	PF00069	0.507
MOD_Plk_1	197	203	PF00069	0.488
MOD_Plk_4	272	278	PF00069	0.498
MOD_Plk_4	54	60	PF00069	0.507
MOD_ProDKin_1	251	257	PF00069	0.372
TRG_DiLeu_BaLyEn_6	167	172	PF01217	0.481
TRG_Pf-PMV_PEXEL_1	17	21	PF00026	0.449
TRG_Pf-PMV_PEXEL_1	170	174	PF00026	0.246

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P6G6	Leptomonas seymouri	70%	100%
A0A0N1HXG1	Leptomonas seymouri	31%	100%
A0A0S4IRD2	Bodo saltans	35%	97%
A0A1X0NWY4	Trypanosomatidae	37%	91%
A0A1X0NY79	Trypanosomatidae	49%	97%
A0A3Q8IEP1	Leishmania donovani	35%	100%
A0A3S5IRB0	Trypanosoma rangeli	45%	100%
A0A3S7WY13	Leishmania donovani	84%	82%
A0A422NJV1	Trypanosoma rangeli	35%	100%
A4HD24	Leishmania braziliensis	35%	99%
A4I0L1	Leishmania infantum	35%	100%
A4I0L2	Leishmania infantum	84%	100%
C9ZVI1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	36%	100%
E9AWH2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	36%	100%
E9AWH3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	82%	100%
O89116	Mus musculus	26%	100%
Q04338	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	27%	100%
Q4QAZ8	Leishmania major	81%	100%
Q4QAZ9	Leishmania major	38%	99%
Q96AJ9	Homo sapiens	24%	100%
Q9JI51	Rattus norvegicus	26%	100%
Q9LVP9	Arabidopsis thaliana	24%	100%
Q9SEL5	Arabidopsis thaliana	21%	100%
V5ARB2	Trypanosoma cruzi	48%	100%
V5BFR7	Trypanosoma cruzi	34%	100%