Putative mitochondrial DNA primase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

mitochondrial DNA primase, putative

Species:

Leishmania braziliensis

UniProt:

A4HCT9_LEIBR

TriTrypDb:

LbrM.23.0780 , LBRM2903_230016600

Length:

534

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005654	nucleoplasm	2	1
GO:0005730	nucleolus	5	1
GO:0005759	mitochondrial matrix	5	1
GO:0020023	kinetoplast	2	1
GO:0031974	membrane-enclosed lumen	2	1
GO:0043226	organelle	2	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1
GO:0043233	organelle lumen	3	1
GO:0070013	intracellular organelle lumen	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4HCT9

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HCT9

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006259	DNA metabolic process	4	1
GO:0006260	DNA replication	5	1
GO:0006261	DNA-templated DNA replication	6	1
GO:0006264	mitochondrial DNA replication	6	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0009987	cellular process	1	1
GO:0032042	mitochondrial DNA metabolic process	5	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0043170	macromolecule metabolic process	3	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044260	obsolete cellular macromolecule metabolic process	3	1
GO:0046483	heterocycle metabolic process	3	1
GO:0071704	organic substance metabolic process	2	1
GO:0090304	nucleic acid metabolic process	4	1
GO:1901360	organic cyclic compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	11
GO:0003899	DNA-directed 5'-3' RNA polymerase activity	6	5
GO:0016740	transferase activity	2	5
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	5
GO:0016779	nucleotidyltransferase activity	4	5
GO:0016787	hydrolase activity	2	11
GO:0016817	hydrolase activity, acting on acid anhydrides	3	11
GO:0034062	5'-3' RNA polymerase activity	5	5
GO:0097747	RNA polymerase activity	4	5
GO:0140098	catalytic activity, acting on RNA	3	5
GO:0140640	catalytic activity, acting on a nucleic acid	2	5
GO:0003896	DNA primase activity	7	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	120	124	PF00656	0.437
CLV_C14_Caspase3-7	349	353	PF00656	0.587
CLV_C14_Caspase3-7	421	425	PF00656	0.334
CLV_MEL_PAP_1	165	171	PF00089	0.241
CLV_NRD_NRD_1	101	103	PF00675	0.687
CLV_NRD_NRD_1	128	130	PF00675	0.372
CLV_NRD_NRD_1	197	199	PF00675	0.494
CLV_NRD_NRD_1	430	432	PF00675	0.393
CLV_NRD_NRD_1	487	489	PF00675	0.561
CLV_NRD_NRD_1	5	7	PF00675	0.648
CLV_NRD_NRD_1	522	524	PF00675	0.712
CLV_NRD_NRD_1	532	534	PF00675	0.709
CLV_PCSK_KEX2_1	128	130	PF00082	0.390
CLV_PCSK_KEX2_1	465	467	PF00082	0.508
CLV_PCSK_KEX2_1	487	489	PF00082	0.561
CLV_PCSK_KEX2_1	532	534	PF00082	0.724
CLV_PCSK_PC1ET2_1	465	467	PF00082	0.449
CLV_PCSK_SKI1_1	198	202	PF00082	0.335
CLV_PCSK_SKI1_1	328	332	PF00082	0.452
CLV_PCSK_SKI1_1	381	385	PF00082	0.349
CLV_PCSK_SKI1_1	409	413	PF00082	0.223
CLV_PCSK_SKI1_1	523	527	PF00082	0.704
DEG_APCC_DBOX_1	197	205	PF00400	0.461
DEG_APCC_KENBOX_2	283	287	PF00400	0.388
DEG_Nend_Nbox_1	1	3	PF02207	0.590
DEG_SCF_FBW7_2	204	210	PF00400	0.384
DEG_SCF_FBW7_2	494	499	PF00400	0.493
DOC_ANK_TNKS_1	5	12	PF00023	0.731
DOC_CKS1_1	141	146	PF01111	0.377
DOC_CKS1_1	169	174	PF01111	0.242
DOC_CKS1_1	204	209	PF01111	0.379
DOC_CYCLIN_RxL_1	325	334	PF00134	0.386
DOC_CYCLIN_yCln2_LP_2	414	420	PF00134	0.324
DOC_MAPK_DCC_7	258	268	PF00069	0.370
DOC_MAPK_gen_1	284	294	PF00069	0.551
DOC_MAPK_gen_1	378	388	PF00069	0.433
DOC_MAPK_gen_1	431	437	PF00069	0.409
DOC_MAPK_gen_1	465	472	PF00069	0.459
DOC_MAPK_HePTP_8	165	177	PF00069	0.339
DOC_MAPK_MEF2A_6	168	177	PF00069	0.342
DOC_MAPK_MEF2A_6	262	270	PF00069	0.372
DOC_MAPK_MEF2A_6	392	399	PF00069	0.277
DOC_MAPK_MEF2A_6	465	474	PF00069	0.475
DOC_MAPK_NFAT4_5	392	400	PF00069	0.295
DOC_PP1_RVXF_1	326	333	PF00149	0.388
DOC_PP2B_LxvP_1	470	473	PF13499	0.486
DOC_PP4_FxxP_1	138	141	PF00568	0.333
DOC_PP4_FxxP_1	294	297	PF00568	0.458
DOC_PP4_FxxP_1	332	335	PF00568	0.392
DOC_USP7_MATH_1	117	121	PF00917	0.602
DOC_USP7_MATH_1	151	155	PF00917	0.491
DOC_USP7_MATH_1	314	318	PF00917	0.351
DOC_USP7_MATH_1	372	376	PF00917	0.507
DOC_USP7_MATH_1	43	47	PF00917	0.813
DOC_USP7_MATH_1	504	508	PF00917	0.810
DOC_USP7_MATH_1	83	87	PF00917	0.551
DOC_USP7_MATH_1	9	13	PF00917	0.735
DOC_USP7_MATH_2	184	190	PF00917	0.303
DOC_USP7_UBL2_3	432	436	PF12436	0.277
DOC_WW_Pin1_4	101	106	PF00397	0.445
DOC_WW_Pin1_4	140	145	PF00397	0.414
DOC_WW_Pin1_4	168	173	PF00397	0.352
DOC_WW_Pin1_4	203	208	PF00397	0.379
DOC_WW_Pin1_4	224	229	PF00397	0.355
DOC_WW_Pin1_4	331	336	PF00397	0.457
DOC_WW_Pin1_4	354	359	PF00397	0.486
DOC_WW_Pin1_4	492	497	PF00397	0.504
DOC_WW_Pin1_4	498	503	PF00397	0.733
LIG_14-3-3_CanoR_1	287	294	PF00244	0.428
LIG_14-3-3_CanoR_1	328	333	PF00244	0.506
LIG_14-3-3_CanoR_1	381	388	PF00244	0.456
LIG_14-3-3_CanoR_1	487	494	PF00244	0.460
LIG_Actin_WH2_2	216	233	PF00022	0.368
LIG_APCC_ABBA_1	291	296	PF00400	0.341
LIG_deltaCOP1_diTrp_1	295	303	PF00928	0.372
LIG_deltaCOP1_diTrp_1	390	394	PF00928	0.296
LIG_deltaCOP1_diTrp_1	443	449	PF00928	0.277
LIG_FHA_1	102	108	PF00498	0.466
LIG_FHA_1	219	225	PF00498	0.463
LIG_FHA_1	286	292	PF00498	0.402
LIG_FHA_1	513	519	PF00498	0.495
LIG_FHA_2	169	175	PF00498	0.461
LIG_FHA_2	28	34	PF00498	0.602
LIG_FHA_2	347	353	PF00498	0.666
LIG_FHA_2	366	372	PF00498	0.405
LIG_FHA_2	382	388	PF00498	0.412
LIG_FHA_2	59	65	PF00498	0.498
LIG_LIR_Apic_2	331	335	PF02991	0.517
LIG_LIR_Gen_1	243	252	PF02991	0.324
LIG_LIR_Gen_1	324	332	PF02991	0.386
LIG_LIR_Gen_1	443	454	PF02991	0.277
LIG_LIR_Nem_3	243	249	PF02991	0.313
LIG_LIR_Nem_3	324	329	PF02991	0.391
LIG_LIR_Nem_3	393	397	PF02991	0.337
LIG_LIR_Nem_3	443	449	PF02991	0.277
LIG_LIR_Nem_3	459	464	PF02991	0.277
LIG_MAD2	199	207	PF02301	0.404
LIG_OCRL_FandH_1	248	260	PF00620	0.430
LIG_Pex14_1	299	303	PF04695	0.359
LIG_Pex14_1	460	464	PF04695	0.277
LIG_Pex14_2	255	259	PF04695	0.335
LIG_SH2_CRK	338	342	PF00017	0.482
LIG_SH2_CRK	464	468	PF00017	0.381
LIG_SH2_NCK_1	338	342	PF00017	0.432
LIG_SH3_3	138	144	PF00018	0.368
LIG_SH3_3	150	156	PF00018	0.388
LIG_SH3_3	166	172	PF00018	0.244
LIG_SH3_3	201	207	PF00018	0.403
LIG_SH3_3	317	323	PF00018	0.445
LIG_SH3_3	355	361	PF00018	0.639
LIG_SUMO_SIM_anti_2	171	178	PF11976	0.394
LIG_SUMO_SIM_par_1	143	150	PF11976	0.356
LIG_TRAF2_1	12	15	PF00917	0.806
LIG_UBA3_1	1	7	PF00899	0.722
LIG_UBA3_1	276	284	PF00899	0.334
LIG_WRC_WIRS_1	329	334	PF05994	0.445
MOD_CDK_SPK_2	331	336	PF00069	0.388
MOD_CDK_SPxK_1	203	209	PF00069	0.384
MOD_CDK_SPxxK_3	101	108	PF00069	0.381
MOD_CDK_SPxxK_3	224	231	PF00069	0.348
MOD_CK1_1	146	152	PF00069	0.428
MOD_CK1_1	25	31	PF00069	0.613
MOD_CK1_1	324	330	PF00069	0.575
MOD_CK1_1	346	352	PF00069	0.605
MOD_CK1_1	41	47	PF00069	0.512
MOD_CK1_1	517	523	PF00069	0.656
MOD_CK1_1	98	104	PF00069	0.603
MOD_CK2_1	27	33	PF00069	0.683
MOD_CK2_1	365	371	PF00069	0.420
MOD_CK2_1	381	387	PF00069	0.412
MOD_CK2_1	58	64	PF00069	0.647
MOD_CK2_1	71	77	PF00069	0.692
MOD_CK2_1	9	15	PF00069	0.683
MOD_CMANNOS	391	394	PF00535	0.277
MOD_DYRK1A_RPxSP_1	168	172	PF00069	0.412
MOD_GlcNHglycan	131	134	PF01048	0.532
MOD_GlcNHglycan	149	152	PF01048	0.402
MOD_GlcNHglycan	288	291	PF01048	0.375
MOD_GlcNHglycan	374	377	PF01048	0.495
MOD_GlcNHglycan	519	522	PF01048	0.657
MOD_GSK3_1	143	150	PF00069	0.426
MOD_GSK3_1	151	158	PF00069	0.397
MOD_GSK3_1	324	331	PF00069	0.494
MOD_GSK3_1	346	353	PF00069	0.609
MOD_GSK3_1	60	67	PF00069	0.755
MOD_GSK3_1	97	104	PF00069	0.585
MOD_N-GLC_1	285	290	PF02516	0.407
MOD_N-GLC_1	64	69	PF02516	0.506
MOD_N-GLC_1	95	100	PF02516	0.709
MOD_N-GLC_2	55	57	PF02516	0.512
MOD_NEK2_1	519	524	PF00069	0.490
MOD_PIKK_1	178	184	PF00454	0.252
MOD_PIKK_1	218	224	PF00454	0.345
MOD_PIKK_1	43	49	PF00454	0.613
MOD_PKA_1	231	237	PF00069	0.329
MOD_PKA_1	487	493	PF00069	0.456
MOD_PKA_2	286	292	PF00069	0.445
MOD_PKA_2	346	352	PF00069	0.733
MOD_PKA_2	486	492	PF00069	0.448
MOD_Plk_1	58	64	PF00069	0.708
MOD_Plk_2-3	22	28	PF00069	0.793
MOD_Plk_4	514	520	PF00069	0.500
MOD_ProDKin_1	101	107	PF00069	0.432
MOD_ProDKin_1	140	146	PF00069	0.417
MOD_ProDKin_1	168	174	PF00069	0.349
MOD_ProDKin_1	203	209	PF00069	0.386
MOD_ProDKin_1	224	230	PF00069	0.361
MOD_ProDKin_1	331	337	PF00069	0.464
MOD_ProDKin_1	354	360	PF00069	0.483
MOD_ProDKin_1	492	498	PF00069	0.505
MOD_SUMO_for_1	208	211	PF00179	0.425
TRG_ENDOCYTIC_2	187	190	PF00928	0.510
TRG_ENDOCYTIC_2	464	467	PF00928	0.366
TRG_ER_diArg_1	128	130	PF00400	0.390
TRG_ER_diArg_1	531	533	PF00400	0.648
TRG_NES_CRM1_1	213	226	PF08389	0.370
TRG_Pf-PMV_PEXEL_1	328	333	PF00026	0.417
TRG_Pf-PMV_PEXEL_1	378	382	PF00026	0.409
TRG_Pf-PMV_PEXEL_1	6	10	PF00026	0.511

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I0R5	Leptomonas seymouri	67%	77%
A0A0S4IY41	Bodo saltans	44%	100%
A0A1X0NXL3	Trypanosomatidae	52%	85%
A0A3S7WXR7	Leishmania donovani	83%	100%
A4I0B9	Leishmania infantum	82%	78%
C9ZV17	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	61%	85%
E9AW83	Leishmania mexicana (strain MHOM/GT/2001/U1103)	83%	100%
Q4QB95	Leishmania major	81%	99%
V5D725	Trypanosoma cruzi	61%	86%