Tryptophan--tRNA ligase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

tryptophanyl-tRNA synthetase, putative

Species:

Leishmania braziliensis

UniProt:

A4HCP4_LEIBR

TriTrypDb:

LbrM.23.0330 , LBRM2903_230009000

Length:

489

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 12
NetGPI	no	yes: 0, no: 12

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4HCP4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HCP4

Function

Biological processes
Term	Name	Level	Count
GO:0006082	organic acid metabolic process	3	13
GO:0006139	nucleobase-containing compound metabolic process	3	13
GO:0006399	tRNA metabolic process	7	13
GO:0006418	tRNA aminoacylation for protein translation	6	13
GO:0006436	tryptophanyl-tRNA aminoacylation	7	13
GO:0006520	amino acid metabolic process	3	13
GO:0006725	cellular aromatic compound metabolic process	3	13
GO:0006807	nitrogen compound metabolic process	2	13
GO:0008152	metabolic process	1	13
GO:0009987	cellular process	1	13
GO:0016070	RNA metabolic process	5	13
GO:0019752	carboxylic acid metabolic process	5	13
GO:0034641	cellular nitrogen compound metabolic process	3	13
GO:0034660	ncRNA metabolic process	6	13
GO:0043038	amino acid activation	4	13
GO:0043039	tRNA aminoacylation	5	13
GO:0043170	macromolecule metabolic process	3	13
GO:0043436	oxoacid metabolic process	4	13
GO:0044237	cellular metabolic process	2	13
GO:0044238	primary metabolic process	2	13
GO:0044281	small molecule metabolic process	2	13
GO:0046483	heterocycle metabolic process	3	13
GO:0071704	organic substance metabolic process	2	13
GO:0090304	nucleic acid metabolic process	4	13
GO:1901360	organic cyclic compound metabolic process	3	13
GO:1901564	organonitrogen compound metabolic process	3	13

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	13
GO:0003824	catalytic activity	1	13
GO:0004812	aminoacyl-tRNA ligase activity	4	13
GO:0004830	tryptophan-tRNA ligase activity	5	13
GO:0005488	binding	1	13
GO:0005524	ATP binding	5	13
GO:0016874	ligase activity	2	13
GO:0016875	ligase activity, forming carbon-oxygen bonds	3	13
GO:0017076	purine nucleotide binding	4	13
GO:0030554	adenyl nucleotide binding	5	13
GO:0032553	ribonucleotide binding	3	13
GO:0032555	purine ribonucleotide binding	4	13
GO:0032559	adenyl ribonucleotide binding	5	13
GO:0035639	purine ribonucleoside triphosphate binding	4	13
GO:0036094	small molecule binding	2	13
GO:0043167	ion binding	2	13
GO:0043168	anion binding	3	13
GO:0097159	organic cyclic compound binding	2	13
GO:0097367	carbohydrate derivative binding	2	13
GO:0140098	catalytic activity, acting on RNA	3	13
GO:0140101	catalytic activity, acting on a tRNA	4	13
GO:0140640	catalytic activity, acting on a nucleic acid	2	13
GO:1901265	nucleoside phosphate binding	3	13
GO:1901363	heterocyclic compound binding	2	13

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	289	293	PF00656	0.549
CLV_NRD_NRD_1	2	4	PF00675	0.592
CLV_NRD_NRD_1	313	315	PF00675	0.316
CLV_NRD_NRD_1	389	391	PF00675	0.270
CLV_NRD_NRD_1	394	396	PF00675	0.257
CLV_PCSK_KEX2_1	2	4	PF00082	0.592
CLV_PCSK_KEX2_1	313	315	PF00082	0.316
CLV_PCSK_KEX2_1	394	396	PF00082	0.297
CLV_PCSK_PC7_1	390	396	PF00082	0.336
CLV_PCSK_SKI1_1	2	6	PF00082	0.700
CLV_PCSK_SKI1_1	220	224	PF00082	0.251
CLV_PCSK_SKI1_1	275	279	PF00082	0.244
CLV_PCSK_SKI1_1	296	300	PF00082	0.262
CLV_PCSK_SKI1_1	313	317	PF00082	0.212
CLV_PCSK_SKI1_1	394	398	PF00082	0.255
CLV_PCSK_SKI1_1	460	464	PF00082	0.427
CLV_PCSK_SKI1_1	485	489	PF00082	0.336
CLV_PCSK_SKI1_1	70	74	PF00082	0.399
DEG_Nend_UBRbox_1	1	4	PF02207	0.522
DEG_SCF_FBW7_1	111	116	PF00400	0.584
DOC_CKS1_1	108	113	PF01111	0.704
DOC_CKS1_1	52	57	PF01111	0.375
DOC_MAPK_FxFP_2	297	300	PF00069	0.481
DOC_MAPK_MEF2A_6	170	178	PF00069	0.411
DOC_PP1_RVXF_1	147	154	PF00149	0.364
DOC_PP4_FxxP_1	207	210	PF00568	0.477
DOC_PP4_FxxP_1	297	300	PF00568	0.481
DOC_USP7_MATH_1	125	129	PF00917	0.504
DOC_USP7_MATH_1	20	24	PF00917	0.544
DOC_USP7_MATH_1	471	475	PF00917	0.546
DOC_USP7_UBL2_3	387	391	PF12436	0.533
DOC_WW_Pin1_4	107	112	PF00397	0.705
DOC_WW_Pin1_4	116	121	PF00397	0.732
DOC_WW_Pin1_4	127	132	PF00397	0.445
DOC_WW_Pin1_4	51	56	PF00397	0.380
LIG_14-3-3_CanoR_1	2	11	PF00244	0.545
LIG_14-3-3_CanoR_1	252	256	PF00244	0.468
LIG_14-3-3_CanoR_1	258	266	PF00244	0.455
LIG_14-3-3_CanoR_1	470	479	PF00244	0.403
LIG_14-3-3_CanoR_1	70	76	PF00244	0.389
LIG_14-3-3_CanoR_1	98	103	PF00244	0.601
LIG_Actin_WH2_2	8	24	PF00022	0.535
LIG_AP2alpha_2	334	336	PF02296	0.477
LIG_BRCT_BRCA1_1	308	312	PF00533	0.453
LIG_FHA_1	283	289	PF00498	0.489
LIG_FHA_1	355	361	PF00498	0.534
LIG_FHA_1	47	53	PF00498	0.532
LIG_FHA_1	97	103	PF00498	0.626
LIG_FHA_2	214	220	PF00498	0.453
LIG_FHA_2	287	293	PF00498	0.549
LIG_FHA_2	379	385	PF00498	0.464
LIG_LIR_Apic_2	205	210	PF02991	0.477
LIG_LIR_Gen_1	417	426	PF02991	0.477
LIG_LIR_Gen_1	430	440	PF02991	0.477
LIG_LIR_Gen_1	54	63	PF02991	0.406
LIG_LIR_Nem_3	17	21	PF02991	0.480
LIG_LIR_Nem_3	265	270	PF02991	0.451
LIG_LIR_Nem_3	309	315	PF02991	0.444
LIG_LIR_Nem_3	334	339	PF02991	0.453
LIG_LIR_Nem_3	417	423	PF02991	0.477
LIG_LIR_Nem_3	430	436	PF02991	0.477
LIG_LIR_Nem_3	54	59	PF02991	0.390
LIG_LRP6_Inhibitor_1	236	242	PF00058	0.281
LIG_LYPXL_S_1	266	270	PF13949	0.312
LIG_LYPXL_yS_3	267	270	PF13949	0.512
LIG_MAD2	314	322	PF02301	0.535
LIG_Pex14_2	308	312	PF04695	0.435
LIG_PTB_Apo_2	256	263	PF02174	0.470
LIG_PTB_Phospho_1	256	262	PF10480	0.470
LIG_REV1ctd_RIR_1	144	153	PF16727	0.386
LIG_REV1ctd_RIR_1	423	432	PF16727	0.377
LIG_SH2_CRK	420	424	PF00017	0.477
LIG_SH2_CRK	68	72	PF00017	0.350
LIG_SH2_STAP1	68	72	PF00017	0.365
LIG_SH2_STAT5	179	182	PF00017	0.449
LIG_SH2_STAT5	202	205	PF00017	0.492
LIG_SH2_STAT5	422	425	PF00017	0.535
LIG_SH3_3	135	141	PF00018	0.495
LIG_SH3_3	168	174	PF00018	0.361
LIG_SH3_3	49	55	PF00018	0.399
LIG_SUMO_SIM_par_1	378	386	PF11976	0.477
LIG_TRAF2_1	373	376	PF00917	0.552
LIG_TYR_ITIM	418	423	PF00017	0.477
LIG_TYR_ITIM	66	71	PF00017	0.352
LIG_UBA3_1	237	242	PF00899	0.487
LIG_WRC_WIRS_1	15	20	PF05994	0.472
LIG_WRC_WIRS_1	72	77	PF05994	0.468
MOD_CK1_1	101	107	PF00069	0.424
MOD_CK1_1	185	191	PF00069	0.390
MOD_CK1_1	25	31	PF00069	0.645
MOD_CK2_1	20	26	PF00069	0.681
MOD_CK2_1	213	219	PF00069	0.451
MOD_CK2_1	369	375	PF00069	0.466
MOD_GlcNHglycan	184	187	PF01048	0.253
MOD_GlcNHglycan	372	375	PF01048	0.256
MOD_GlcNHglycan	397	400	PF01048	0.244
MOD_GlcNHglycan	446	449	PF01048	0.289
MOD_GlcNHglycan	81	85	PF01048	0.314
MOD_GSK3_1	109	116	PF00069	0.528
MOD_GSK3_1	16	23	PF00069	0.645
MOD_GSK3_1	278	285	PF00069	0.500
MOD_GSK3_1	378	385	PF00069	0.510
MOD_GSK3_1	96	103	PF00069	0.587
MOD_LATS_1	96	102	PF00433	0.360
MOD_N-GLC_2	480	482	PF02516	0.244
MOD_NEK2_1	177	182	PF00069	0.513
MOD_NEK2_1	257	262	PF00069	0.457
MOD_NEK2_1	273	278	PF00069	0.389
MOD_NEK2_1	337	342	PF00069	0.475
MOD_NEK2_1	354	359	PF00069	0.388
MOD_NEK2_1	407	412	PF00069	0.553
MOD_NEK2_2	378	383	PF00069	0.493
MOD_NEK2_2	7	12	PF00069	0.455
MOD_PIKK_1	299	305	PF00454	0.512
MOD_PIKK_1	354	360	PF00454	0.377
MOD_PIKK_1	434	440	PF00454	0.549
MOD_PK_1	98	104	PF00069	0.387
MOD_PKA_1	2	8	PF00069	0.517
MOD_PKA_2	2	8	PF00069	0.517
MOD_PKA_2	21	27	PF00069	0.531
MOD_PKA_2	251	257	PF00069	0.468
MOD_PKA_2	441	447	PF00069	0.377
MOD_Plk_1	165	171	PF00069	0.576
MOD_Plk_2-3	46	52	PF00069	0.432
MOD_Plk_4	172	178	PF00069	0.515
MOD_Plk_4	251	257	PF00069	0.451
MOD_Plk_4	266	272	PF00069	0.421
MOD_ProDKin_1	107	113	PF00069	0.709
MOD_ProDKin_1	116	122	PF00069	0.731
MOD_ProDKin_1	127	133	PF00069	0.445
MOD_ProDKin_1	51	57	PF00069	0.383
MOD_SUMO_for_1	75	78	PF00179	0.417
MOD_SUMO_rev_2	217	222	PF00179	0.444
MOD_SUMO_rev_2	447	453	PF00179	0.516
TRG_DiLeu_BaEn_2	172	178	PF01217	0.527
TRG_ENDOCYTIC_2	267	270	PF00928	0.450
TRG_ENDOCYTIC_2	420	423	PF00928	0.477
TRG_ENDOCYTIC_2	68	71	PF00928	0.343
TRG_ER_diArg_1	1	3	PF00400	0.566
TRG_ER_diArg_1	312	314	PF00400	0.516
TRG_ER_diArg_1	393	395	PF00400	0.495
TRG_NLS_MonoExtC_3	389	394	PF00514	0.466
TRG_NLS_MonoExtN_4	387	394	PF00514	0.514
TRG_Pf-PMV_PEXEL_1	35	39	PF00026	0.535

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P3G3	Leptomonas seymouri	38%	100%
A0A0N1HXL2	Leptomonas seymouri	73%	100%
A0A0S4IN16	Bodo saltans	46%	100%
A0A0S4JC70	Bodo saltans	52%	100%
A0A1X0NGJ6	Trypanosomatidae	54%	100%
A0A1X0NZB4	Trypanosomatidae	39%	100%
A0A3R7MPN7	Trypanosoma rangeli	47%	100%
A0A3S7WXL2	Leishmania donovani	80%	100%
A0A3S7X238	Leishmania donovani	38%	100%
A0A422NW64	Trypanosoma rangeli	54%	100%
A2BLD4	Hyperthermus butylicus (strain DSM 5456 / JCM 9403 / PLM1-5)	27%	100%
A3MX72	Pyrobaculum calidifontis (strain DSM 21063 / JCM 11548 / VA1)	36%	100%
A4HH14	Leishmania braziliensis	38%	100%
A4I070	Leishmania infantum	80%	100%
A4I443	Leishmania infantum	38%	100%
A4WL99	Pyrobaculum arsenaticum (strain DSM 13514 / JCM 11321 / PZ6)	36%	100%
C6A032	Thermococcus sibiricus (strain DSM 12597 / MM 739)	34%	100%
C9ZKR9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	41%	100%
E9ADK8	Leishmania major	39%	100%
E9AM73	Leishmania mexicana (strain MHOM/GT/2001/U1103)	38%	100%
E9AW33	Leishmania mexicana (strain MHOM/GT/2001/U1103)	81%	100%
O26352	Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)	27%	100%
O59584	Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)	38%	100%
O96771	Encephalitozoon cuniculi (strain GB-M1)	41%	100%
P17248	Bos taurus	40%	100%
P23381	Homo sapiens	38%	100%
P23612	Oryctolagus cuniculus	39%	100%
P32921	Mus musculus	39%	100%
Q09692	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	40%	100%
Q12109	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	45%	100%
Q4JBG7	Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)	40%	100%
Q4QBE4	Leishmania major	82%	100%
Q55DZ8	Dictyostelium discoideum	43%	100%
Q58810	Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)	29%	100%
Q5R4J1	Pongo abelii	38%	100%
Q6P7B0	Rattus norvegicus	39%	100%
Q8TYF7	Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)	27%	100%
Q8U453	Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)	38%	100%
Q8ZTU5	Pyrobaculum aerophilum (strain ATCC 51768 / DSM 7523 / JCM 9630 / CIP 104966 / NBRC 100827 / IM2)	37%	100%
Q976M1	Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)	39%	100%
Q97ZX0	Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)	34%	100%
Q9HN66	Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)	36%	100%
Q9SR15	Arabidopsis thaliana	36%	100%
Q9UY11	Pyrococcus abyssi (strain GE5 / Orsay)	35%	100%
Q9Y924	Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)	29%	100%
V5B4S5	Trypanosoma cruzi	46%	100%