Checkpoint protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Checkpoint protein

Gene product:

Checkpoint protein HUS1, putative

Species:

Leishmania braziliensis

UniProt:

A4HCP3_LEIBR

TriTrypDb:

LbrM.23.0320 , LBRM2903_230008900

Length:

323

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 8
NetGPI	no	yes: 0, no: 8

Cellular components
Term	Name	Level	Count
GO:0005730	nucleolus	5	9
GO:0016020	membrane	2	5
GO:0030896	checkpoint clamp complex	3	9
GO:0032991	protein-containing complex	1	9
GO:0043226	organelle	2	9
GO:0043228	non-membrane-bounded organelle	3	9
GO:0043229	intracellular organelle	3	9
GO:0043232	intracellular non-membrane-bounded organelle	4	9
GO:0110165	cellular anatomical entity	1	9
GO:0140513	nuclear protein-containing complex	2	9
GO:0005634	nucleus	5	1
GO:0035861	site of double-strand break	3	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0090734	site of DNA damage	2	1

Expansion

Sequence features

A4HCP3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HCP3

Function

Biological processes
Term	Name	Level	Count
GO:0000075	cell cycle checkpoint signaling	4	9
GO:0000077	DNA damage checkpoint signaling	5	9
GO:0006950	response to stress	2	9
GO:0006974	DNA damage response	4	9
GO:0007165	signal transduction	2	9
GO:0009987	cellular process	1	9
GO:0010564	regulation of cell cycle process	5	9
GO:0010948	negative regulation of cell cycle process	6	9
GO:0031570	DNA integrity checkpoint signaling	5	9
GO:0033554	cellular response to stress	3	9
GO:0035556	intracellular signal transduction	3	9
GO:0042770	signal transduction in response to DNA damage	4	9
GO:0045786	negative regulation of cell cycle	5	9
GO:0048519	negative regulation of biological process	3	9
GO:0048523	negative regulation of cellular process	4	9
GO:0050789	regulation of biological process	2	9
GO:0050794	regulation of cellular process	3	9
GO:0050896	response to stimulus	1	9
GO:0051716	cellular response to stimulus	2	9
GO:0051726	regulation of cell cycle	4	9
GO:0065007	biological regulation	1	9
GO:1901987	regulation of cell cycle phase transition	6	9
GO:1901988	negative regulation of cell cycle phase transition	7	9
GO:0000076	DNA replication checkpoint signaling	6	1
GO:0000723	telomere maintenance	5	1
GO:0000724	double-strand break repair via homologous recombination	7	1
GO:0000725	recombinational repair	6	1
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006259	DNA metabolic process	4	1
GO:0006281	DNA repair	5	1
GO:0006289	nucleotide-excision repair	6	1
GO:0006302	double-strand break repair	6	1
GO:0006310	DNA recombination	5	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0006996	organelle organization	4	1
GO:0007093	mitotic cell cycle checkpoint signaling	4	1
GO:0007346	regulation of mitotic cell cycle	5	1
GO:0008152	metabolic process	1	1
GO:0010389	regulation of G2/M transition of mitotic cell cycle	7	1
GO:0010972	negative regulation of G2/M transition of mitotic cell cycle	8	1
GO:0016043	cellular component organization	3	1
GO:0022402	cell cycle process	2	1
GO:0022414	reproductive process	1	1
GO:0031573	mitotic intra-S DNA damage checkpoint signaling	7	1
GO:0032200	telomere organization	6	1
GO:0033313	meiotic cell cycle checkpoint signaling	3	1
GO:0033314	mitotic DNA replication checkpoint signaling	6	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0043170	macromolecule metabolic process	3	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044260	obsolete cellular macromolecule metabolic process	3	1
GO:0044773	mitotic DNA damage checkpoint signaling	6	1
GO:0044774	mitotic DNA integrity checkpoint signaling	5	1
GO:0044778	meiotic DNA integrity checkpoint signaling	4	1
GO:0044818	mitotic G2/M transition checkpoint	5	1
GO:0045930	negative regulation of mitotic cell cycle	6	1
GO:0046483	heterocycle metabolic process	3	1
GO:0051276	chromosome organization	5	1
GO:0071704	organic substance metabolic process	2	1
GO:0071840	cellular component organization or biogenesis	2	1
GO:0090304	nucleic acid metabolic process	4	1
GO:1901360	organic cyclic compound metabolic process	3	1
GO:1901990	regulation of mitotic cell cycle phase transition	6	1
GO:1901991	negative regulation of mitotic cell cycle phase transition	7	1
GO:1902749	regulation of cell cycle G2/M phase transition	7	1
GO:1902750	negative regulation of cell cycle G2/M phase transition	8	1
GO:1903046	meiotic cell cycle process	2	1
GO:1903047	mitotic cell cycle process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	1
GO:0003677	DNA binding	4	1
GO:0003684	damaged DNA binding	5	1
GO:0005488	binding	1	1
GO:0097159	organic cyclic compound binding	2	1
GO:1901363	heterocyclic compound binding	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	178	182	PF00656	0.521
CLV_C14_Caspase3-7	197	201	PF00656	0.521
CLV_C14_Caspase3-7	6	10	PF00656	0.517
CLV_NRD_NRD_1	275	277	PF00675	0.313
CLV_PCSK_KEX2_1	210	212	PF00082	0.324
CLV_PCSK_PC1ET2_1	210	212	PF00082	0.321
CLV_PCSK_SKI1_1	143	147	PF00082	0.251
CLV_PCSK_SKI1_1	188	192	PF00082	0.235
CLV_PCSK_SKI1_1	215	219	PF00082	0.264
CLV_PCSK_SKI1_1	8	12	PF00082	0.327
DEG_APCC_DBOX_1	113	121	PF00400	0.509
DEG_APCC_DBOX_1	154	162	PF00400	0.457
DEG_Nend_UBRbox_1	1	4	PF02207	0.454
DOC_ANK_TNKS_1	192	199	PF00023	0.396
DOC_CKS1_1	189	194	PF01111	0.514
DOC_MAPK_gen_1	210	220	PF00069	0.509
DOC_MAPK_gen_1	8	16	PF00069	0.582
DOC_MAPK_MEF2A_6	155	162	PF00069	0.521
DOC_MAPK_MEF2A_6	286	295	PF00069	0.424
DOC_PP1_RVXF_1	33	39	PF00149	0.521
DOC_PP2B_LxvP_1	148	151	PF13499	0.566
DOC_PP4_FxxP_1	133	136	PF00568	0.521
DOC_USP7_MATH_1	129	133	PF00917	0.491
DOC_USP7_MATH_1	179	183	PF00917	0.586
DOC_USP7_MATH_1	219	223	PF00917	0.456
DOC_USP7_UBL2_3	4	8	PF12436	0.514
DOC_WW_Pin1_4	146	151	PF00397	0.509
DOC_WW_Pin1_4	188	193	PF00397	0.514
LIG_14-3-3_CanoR_1	2	8	PF00244	0.517
LIG_14-3-3_CanoR_1	21	29	PF00244	0.451
LIG_14-3-3_CanoR_1	233	237	PF00244	0.521
LIG_14-3-3_CanoR_1	280	288	PF00244	0.479
LIG_14-3-3_CanoR_1	30	39	PF00244	0.606
LIG_14-3-3_CanoR_1	53	59	PF00244	0.599
LIG_Actin_WH2_2	161	178	PF00022	0.521
LIG_APCC_ABBA_1	223	228	PF00400	0.521
LIG_BIR_III_4	9	13	PF00653	0.521
LIG_BRCT_BRCA1_1	181	185	PF00533	0.509
LIG_BRCT_BRCA1_1	56	60	PF00533	0.521
LIG_eIF4E_1	238	244	PF01652	0.521
LIG_FHA_1	167	173	PF00498	0.521
LIG_FHA_1	189	195	PF00498	0.435
LIG_FHA_1	268	274	PF00498	0.479
LIG_FHA_1	311	317	PF00498	0.293
LIG_FHA_1	73	79	PF00498	0.516
LIG_FHA_2	168	174	PF00498	0.473
LIG_FHA_2	176	182	PF00498	0.434
LIG_FHA_2	4	10	PF00498	0.479
LIG_Integrin_isoDGR_2	112	114	PF01839	0.308
LIG_LIR_Apic_2	130	136	PF02991	0.479
LIG_LIR_Gen_1	235	244	PF02991	0.521
LIG_LIR_Gen_1	275	285	PF02991	0.528
LIG_LIR_Gen_1	57	64	PF02991	0.457
LIG_LIR_Nem_3	235	241	PF02991	0.521
LIG_LIR_Nem_3	275	281	PF02991	0.528
LIG_PCNA_yPIPBox_3	312	322	PF02747	0.389
LIG_Pex14_2	226	230	PF04695	0.483
LIG_Pex14_2	278	282	PF04695	0.521
LIG_SH2_NCK_1	238	242	PF00017	0.521
LIG_SH2_SRC	318	321	PF00017	0.385
LIG_SH2_SRC	69	72	PF00017	0.509
LIG_SH2_STAT5	296	299	PF00017	0.521
LIG_SH2_STAT5	318	321	PF00017	0.387
LIG_SH3_3	123	129	PF00018	0.396
LIG_SH3_3	186	192	PF00018	0.521
LIG_SH3_3	206	212	PF00018	0.509
LIG_SUMO_SIM_anti_2	239	245	PF11976	0.592
LIG_SUMO_SIM_par_1	164	170	PF11976	0.521
LIG_SUMO_SIM_par_1	267	275	PF11976	0.479
LIG_TYR_ITIM	236	241	PF00017	0.476
LIG_UBA3_1	84	89	PF00899	0.521
LIG_WRC_WIRS_1	168	173	PF05994	0.521
MOD_CDK_SPK_2	188	193	PF00069	0.492
MOD_CK1_1	267	273	PF00069	0.396
MOD_CK2_1	64	70	PF00069	0.396
MOD_DYRK1A_RPxSP_1	188	192	PF00069	0.435
MOD_GlcNHglycan	185	188	PF01048	0.321
MOD_GlcNHglycan	282	285	PF01048	0.395
MOD_GlcNHglycan	66	69	PF01048	0.308
MOD_GSK3_1	175	182	PF00069	0.509
MOD_GSK3_1	54	61	PF00069	0.465
MOD_GSK3_1	94	101	PF00069	0.477
MOD_NEK2_1	166	171	PF00069	0.506
MOD_NEK2_1	175	180	PF00069	0.451
MOD_NEK2_1	291	296	PF00069	0.521
MOD_NEK2_1	3	8	PF00069	0.488
MOD_NEK2_1	303	308	PF00069	0.240
MOD_NEK2_1	39	44	PF00069	0.524
MOD_NEK2_2	219	224	PF00069	0.541
MOD_NEK2_2	232	237	PF00069	0.512
MOD_PIKK_1	20	26	PF00454	0.396
MOD_PKA_2	20	26	PF00069	0.396
MOD_PKA_2	232	238	PF00069	0.509
MOD_PKB_1	35	43	PF00069	0.479
MOD_Plk_1	129	135	PF00069	0.521
MOD_Plk_1	166	172	PF00069	0.479
MOD_Plk_1	267	273	PF00069	0.489
MOD_Plk_1	70	76	PF00069	0.476
MOD_Plk_2-3	167	173	PF00069	0.521
MOD_Plk_4	167	173	PF00069	0.481
MOD_Plk_4	267	273	PF00069	0.560
MOD_Plk_4	303	309	PF00069	0.326
MOD_Plk_4	54	60	PF00069	0.456
MOD_ProDKin_1	146	152	PF00069	0.509
MOD_ProDKin_1	188	194	PF00069	0.514
MOD_SUMO_for_1	236	239	PF00179	0.521
MOD_SUMO_rev_2	142	148	PF00179	0.396
TRG_DiLeu_BaEn_1	239	244	PF01217	0.521
TRG_DiLeu_BaEn_2	166	172	PF01217	0.454
TRG_ENDOCYTIC_2	238	241	PF00928	0.476
TRG_ER_diArg_1	212	215	PF00400	0.524
TRG_ER_diArg_1	35	38	PF00400	0.454
TRG_NES_CRM1_1	216	228	PF08389	0.388
TRG_NES_CRM1_1	287	299	PF08389	0.521
TRG_NLS_MonoCore_2	209	214	PF00514	0.492
TRG_Pf-PMV_PEXEL_1	138	142	PF00026	0.254

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I495	Leptomonas seymouri	62%	96%
A0A1X0NG57	Trypanosomatidae	34%	99%
A0A3S5H7B6	Leishmania donovani	84%	98%
A0A422NW79	Trypanosoma rangeli	39%	100%
A4I069	Leishmania infantum	84%	98%
E9AW32	Leishmania mexicana (strain MHOM/GT/2001/U1103)	85%	98%
Q4QBE5	Leishmania major	84%	100%