EF-hand domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein, conserved

Species:

Leishmania braziliensis

UniProt:

A4HCE3_LEIBR

TriTrypDb:

LbrM.22.0820 , LBRM2903_220013700

Length:

868

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 1, no: 5
NetGPI	no	yes: 0, no: 6

Cellular components
Term	Name	Level	Count
GO:0032991	protein-containing complex	1	1
GO:0034702	monoatomic ion channel complex	4	1
GO:0034703	cation channel complex	5	1
GO:0034704	calcium channel complex	6	1
GO:0098796	membrane protein complex	2	1
GO:0098798	mitochondrial protein-containing complex	2	1
GO:0098800	inner mitochondrial membrane protein complex	3	1
GO:1902495	transmembrane transporter complex	3	1
GO:1990246	uniplex complex	4	1
GO:1990351	transporter complex	2	1

Expansion

Sequence features

A4HCE3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HCE3

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	7
GO:0006811	monoatomic ion transport	4	7
GO:0006812	monoatomic cation transport	5	7
GO:0006816	calcium ion transport	7	7
GO:0006851	mitochondrial calcium ion transmembrane transport	4	7
GO:0009987	cellular process	1	7
GO:0030001	metal ion transport	6	7
GO:0034220	monoatomic ion transmembrane transport	3	7
GO:0051179	localization	1	7
GO:0051234	establishment of localization	2	7
GO:0055085	transmembrane transport	2	7
GO:0070588	calcium ion transmembrane transport	6	7
GO:0098655	monoatomic cation transmembrane transport	4	7
GO:0098660	inorganic ion transmembrane transport	4	7
GO:0098662	inorganic cation transmembrane transport	5	7
GO:1990542	mitochondrial transmembrane transport	3	7
GO:0006873	intracellular monoatomic ion homeostasis	4	1
GO:0006874	intracellular calcium ion homeostasis	7	1
GO:0006875	obsolete intracellular metal ion homeostasis	6	1
GO:0019725	cellular homeostasis	2	1
GO:0030003	intracellular monoatomic cation homeostasis	5	1
GO:0036444	calcium import into the mitochondrion	5	1
GO:0042592	homeostatic process	3	1
GO:0048878	chemical homeostasis	4	1
GO:0050801	monoatomic ion homeostasis	5	1
GO:0051560	mitochondrial calcium ion homeostasis	8	1
GO:0055065	obsolete metal ion homeostasis	7	1
GO:0055074	calcium ion homeostasis	8	1
GO:0055080	monoatomic cation homeostasis	6	1
GO:0055082	intracellular chemical homeostasis	3	1
GO:0065007	biological regulation	1	1
GO:0065008	regulation of biological quality	2	1
GO:0072503	obsolete cellular divalent inorganic cation homeostasis	6	1
GO:0072507	obsolete divalent inorganic cation homeostasis	7	1
GO:0098771	inorganic ion homeostasis	6	1

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	7
GO:0005509	calcium ion binding	5	7
GO:0043167	ion binding	2	7
GO:0043169	cation binding	3	7
GO:0046872	metal ion binding	4	7

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	255	259	PF00656	0.529
CLV_C14_Caspase3-7	331	335	PF00656	0.359
CLV_C14_Caspase3-7	821	825	PF00656	0.621
CLV_NRD_NRD_1	273	275	PF00675	0.474
CLV_NRD_NRD_1	440	442	PF00675	0.720
CLV_NRD_NRD_1	484	486	PF00675	0.611
CLV_NRD_NRD_1	5	7	PF00675	0.625
CLV_NRD_NRD_1	620	622	PF00675	0.520
CLV_PCSK_KEX2_1	173	175	PF00082	0.501
CLV_PCSK_KEX2_1	272	274	PF00082	0.501
CLV_PCSK_KEX2_1	440	442	PF00082	0.720
CLV_PCSK_KEX2_1	5	7	PF00082	0.625
CLV_PCSK_KEX2_1	620	622	PF00082	0.520
CLV_PCSK_KEX2_1	853	855	PF00082	0.683
CLV_PCSK_PC1ET2_1	173	175	PF00082	0.501
CLV_PCSK_PC1ET2_1	853	855	PF00082	0.683
CLV_PCSK_PC7_1	268	274	PF00082	0.453
CLV_PCSK_SKI1_1	143	147	PF00082	0.542
CLV_PCSK_SKI1_1	154	158	PF00082	0.532
CLV_PCSK_SKI1_1	211	215	PF00082	0.454
CLV_PCSK_SKI1_1	463	467	PF00082	0.466
CLV_PCSK_SKI1_1	486	490	PF00082	0.602
CLV_PCSK_SKI1_1	5	9	PF00082	0.691
CLV_PCSK_SKI1_1	620	624	PF00082	0.495
CLV_PCSK_SKI1_1	811	815	PF00082	0.595
CLV_Separin_Metazoa	460	464	PF03568	0.553
CLV_Separin_Metazoa	684	688	PF03568	0.433
DEG_APCC_DBOX_1	594	602	PF00400	0.441
DEG_SCF_FBW7_1	316	322	PF00400	0.525
DEG_SCF_FBW7_1	400	407	PF00400	0.557
DEG_SCF_FBW7_1	527	533	PF00400	0.720
DEG_SPOP_SBC_1	390	394	PF00917	0.575
DEG_SPOP_SBC_1	493	497	PF00917	0.652
DOC_CKS1_1	316	321	PF01111	0.584
DOC_CKS1_1	401	406	PF01111	0.675
DOC_CKS1_1	527	532	PF01111	0.719
DOC_CYCLIN_RxL_1	3	12	PF00134	0.539
DOC_CYCLIN_RxL_1	808	816	PF00134	0.564
DOC_CYCLIN_yCln2_LP_2	461	467	PF00134	0.577
DOC_MAPK_gen_1	272	281	PF00069	0.390
DOC_MAPK_MEF2A_6	662	671	PF00069	0.529
DOC_MAPK_MEF2A_6	795	802	PF00069	0.345
DOC_PP1_RVXF_1	597	604	PF00149	0.439
DOC_PP1_RVXF_1	747	753	PF00149	0.527
DOC_PP2B_LxvP_1	214	217	PF13499	0.480
DOC_PP2B_LxvP_1	24	27	PF13499	0.525
DOC_PP2B_LxvP_1	279	282	PF13499	0.347
DOC_PP4_FxxP_1	849	852	PF00568	0.616
DOC_USP7_MATH_1	127	131	PF00917	0.734
DOC_USP7_MATH_1	16	20	PF00917	0.706
DOC_USP7_MATH_1	264	268	PF00917	0.427
DOC_USP7_MATH_1	362	366	PF00917	0.615
DOC_USP7_MATH_1	377	381	PF00917	0.716
DOC_USP7_MATH_1	382	386	PF00917	0.667
DOC_USP7_MATH_1	404	408	PF00917	0.583
DOC_USP7_MATH_1	420	424	PF00917	0.681
DOC_USP7_MATH_1	425	429	PF00917	0.742
DOC_USP7_MATH_1	493	497	PF00917	0.637
DOC_USP7_MATH_1	530	534	PF00917	0.760
DOC_USP7_MATH_1	547	551	PF00917	0.534
DOC_USP7_MATH_1	553	557	PF00917	0.634
DOC_USP7_MATH_1	561	565	PF00917	0.509
DOC_USP7_MATH_1	736	740	PF00917	0.600
DOC_USP7_MATH_1	809	813	PF00917	0.553
DOC_USP7_MATH_1	9	13	PF00917	0.576
DOC_WW_Pin1_4	123	128	PF00397	0.761
DOC_WW_Pin1_4	27	32	PF00397	0.728
DOC_WW_Pin1_4	315	320	PF00397	0.574
DOC_WW_Pin1_4	375	380	PF00397	0.799
DOC_WW_Pin1_4	384	389	PF00397	0.673
DOC_WW_Pin1_4	396	401	PF00397	0.629
DOC_WW_Pin1_4	406	411	PF00397	0.745
DOC_WW_Pin1_4	526	531	PF00397	0.758
DOC_WW_Pin1_4	824	829	PF00397	0.491
LIG_14-3-3_CanoR_1	143	152	PF00244	0.618
LIG_14-3-3_CanoR_1	272	278	PF00244	0.566
LIG_14-3-3_CanoR_1	565	572	PF00244	0.574
LIG_14-3-3_CanoR_1	713	723	PF00244	0.665
LIG_14-3-3_CanoR_1	749	753	PF00244	0.525
LIG_14-3-3_CanoR_1	795	801	PF00244	0.550
LIG_Actin_WH2_2	584	601	PF00022	0.440
LIG_Actin_WH2_2	671	689	PF00022	0.617
LIG_Actin_WH2_2	700	715	PF00022	0.552
LIG_BIR_III_2	676	680	PF00653	0.611
LIG_BIR_III_2	726	730	PF00653	0.533
LIG_BRCT_BRCA1_1	245	249	PF00533	0.819
LIG_CSL_BTD_1	527	530	PF09270	0.619
LIG_deltaCOP1_diTrp_1	340	343	PF00928	0.562
LIG_deltaCOP1_diTrp_1	509	516	PF00928	0.453
LIG_FHA_1	197	203	PF00498	0.482
LIG_FHA_1	347	353	PF00498	0.561
LIG_FHA_1	434	440	PF00498	0.599
LIG_FHA_1	496	502	PF00498	0.515
LIG_FHA_1	543	549	PF00498	0.721
LIG_FHA_1	609	615	PF00498	0.584
LIG_FHA_1	698	704	PF00498	0.542
LIG_FHA_1	76	82	PF00498	0.682
LIG_FHA_1	94	100	PF00498	0.384
LIG_FHA_2	116	122	PF00498	0.697
LIG_FHA_2	144	150	PF00498	0.685
LIG_FHA_2	155	161	PF00498	0.759
LIG_FHA_2	253	259	PF00498	0.567
LIG_FHA_2	479	485	PF00498	0.718
LIG_FHA_2	715	721	PF00498	0.427
LIG_Integrin_isoDGR_2	335	337	PF01839	0.331
LIG_LIR_Apic_2	398	404	PF02991	0.569
LIG_LIR_Gen_1	20	31	PF02991	0.734
LIG_LIR_Gen_1	308	316	PF02991	0.445
LIG_LIR_Gen_1	340	351	PF02991	0.458
LIG_LIR_Gen_1	542	551	PF02991	0.508
LIG_LIR_Gen_1	583	591	PF02991	0.287
LIG_LIR_Gen_1	653	661	PF02991	0.353
LIG_LIR_Gen_1	789	798	PF02991	0.565
LIG_LIR_Gen_1	830	837	PF02991	0.496
LIG_LIR_Nem_3	187	192	PF02991	0.504
LIG_LIR_Nem_3	20	26	PF02991	0.664
LIG_LIR_Nem_3	308	312	PF02991	0.441
LIG_LIR_Nem_3	324	330	PF02991	0.346
LIG_LIR_Nem_3	340	346	PF02991	0.542
LIG_LIR_Nem_3	525	531	PF02991	0.663
LIG_LIR_Nem_3	542	546	PF02991	0.467
LIG_LIR_Nem_3	583	587	PF02991	0.448
LIG_LIR_Nem_3	650	654	PF02991	0.491
LIG_LIR_Nem_3	789	793	PF02991	0.457
LIG_LIR_Nem_3	812	817	PF02991	0.595
LIG_LIR_Nem_3	830	834	PF02991	0.356
LIG_NRBOX	519	525	PF00104	0.455
LIG_Pex14_2	289	293	PF04695	0.432
LIG_Pex14_2	309	313	PF04695	0.244
LIG_SH2_CRK	401	405	PF00017	0.572
LIG_SH2_CRK	543	547	PF00017	0.505
LIG_SH2_CRK	654	658	PF00017	0.389
LIG_SH2_CRK	85	89	PF00017	0.431
LIG_SH2_STAP1	275	279	PF00017	0.365
LIG_SH2_STAP1	306	310	PF00017	0.432
LIG_SH2_STAP1	360	364	PF00017	0.413
LIG_SH2_STAP1	483	487	PF00017	0.604
LIG_SH2_STAP1	531	535	PF00017	0.507
LIG_SH2_STAP1	584	588	PF00017	0.291
LIG_SH2_STAT3	862	865	PF00017	0.495
LIG_SH2_STAT5	23	26	PF00017	0.632
LIG_SH2_STAT5	593	596	PF00017	0.440
LIG_SH2_STAT5	862	865	PF00017	0.495
LIG_SH3_3	28	34	PF00018	0.598
LIG_SH3_3	524	530	PF00018	0.704
LIG_SH3_3	646	652	PF00018	0.362
LIG_SH3_3	676	682	PF00018	0.505
LIG_SUMO_SIM_anti_2	799	804	PF11976	0.455
LIG_SUMO_SIM_par_1	201	207	PF11976	0.471
LIG_SUMO_SIM_par_1	212	219	PF11976	0.497
LIG_SUMO_SIM_par_1	348	353	PF11976	0.564
LIG_SUMO_SIM_par_1	6	12	PF11976	0.501
LIG_SUMO_SIM_par_1	796	801	PF11976	0.455
LIG_TRAF2_1	145	148	PF00917	0.542
LIG_TRAF2_1	718	721	PF00917	0.538
LIG_WRC_WIRS_1	312	317	PF05994	0.449
LIG_WRC_WIRS_1	584	589	PF05994	0.322
MOD_CDK_SPK_2	400	405	PF00069	0.564
MOD_CK1_1	116	122	PF00069	0.713
MOD_CK1_1	130	136	PF00069	0.659
MOD_CK1_1	163	169	PF00069	0.675
MOD_CK1_1	231	237	PF00069	0.578
MOD_CK1_1	378	384	PF00069	0.699
MOD_CK1_1	423	429	PF00069	0.583
MOD_CK1_1	496	502	PF00069	0.594
MOD_CK1_1	522	528	PF00069	0.515
MOD_CK1_1	554	560	PF00069	0.617
MOD_CK1_1	564	570	PF00069	0.560
MOD_CK1_1	583	589	PF00069	0.514
MOD_CK1_1	644	650	PF00069	0.403
MOD_CK2_1	115	121	PF00069	0.674
MOD_CK2_1	143	149	PF00069	0.660
MOD_CK2_1	154	160	PF00069	0.766
MOD_CK2_1	256	262	PF00069	0.451
MOD_CK2_1	714	720	PF00069	0.422
MOD_CK2_1	736	742	PF00069	0.553
MOD_CK2_1	758	764	PF00069	0.667
MOD_CK2_1	804	810	PF00069	0.503
MOD_CK2_1	824	830	PF00069	0.260
MOD_CMANNOS	513	516	PF00535	0.698
MOD_GlcNHglycan	11	14	PF01048	0.653
MOD_GlcNHglycan	115	118	PF01048	0.593
MOD_GlcNHglycan	163	166	PF01048	0.673
MOD_GlcNHglycan	230	233	PF01048	0.771
MOD_GlcNHglycan	236	239	PF01048	0.716
MOD_GlcNHglycan	364	367	PF01048	0.725
MOD_GlcNHglycan	380	383	PF01048	0.568
MOD_GlcNHglycan	384	387	PF01048	0.702
MOD_GlcNHglycan	425	428	PF01048	0.783
MOD_GlcNHglycan	45	48	PF01048	0.622
MOD_GlcNHglycan	498	501	PF01048	0.584
MOD_GlcNHglycan	509	512	PF01048	0.611
MOD_GlcNHglycan	55	58	PF01048	0.625
MOD_GlcNHglycan	553	556	PF01048	0.564
MOD_GlcNHglycan	643	646	PF01048	0.472
MOD_GlcNHglycan	68	71	PF01048	0.494
MOD_GlcNHglycan	709	712	PF01048	0.420
MOD_GlcNHglycan	760	763	PF01048	0.592
MOD_GSK3_1	112	119	PF00069	0.691
MOD_GSK3_1	123	130	PF00069	0.714
MOD_GSK3_1	156	163	PF00069	0.705
MOD_GSK3_1	228	235	PF00069	0.779
MOD_GSK3_1	249	256	PF00069	0.748
MOD_GSK3_1	264	271	PF00069	0.507
MOD_GSK3_1	311	318	PF00069	0.397
MOD_GSK3_1	377	384	PF00069	0.795
MOD_GSK3_1	388	395	PF00069	0.656
MOD_GSK3_1	396	403	PF00069	0.525
MOD_GSK3_1	425	432	PF00069	0.704
MOD_GSK3_1	451	458	PF00069	0.624
MOD_GSK3_1	492	499	PF00069	0.508
MOD_GSK3_1	522	529	PF00069	0.734
MOD_GSK3_1	547	554	PF00069	0.695
MOD_GSK3_1	608	615	PF00069	0.590
MOD_GSK3_1	730	737	PF00069	0.765
MOD_GSK3_1	820	827	PF00069	0.512
MOD_LATS_1	141	147	PF00433	0.542
MOD_N-GLC_1	130	135	PF02516	0.586
MOD_NEK2_1	298	303	PF00069	0.396
MOD_NEK2_1	346	351	PF00069	0.513
MOD_NEK2_1	641	646	PF00069	0.680
MOD_NEK2_1	8	13	PF00069	0.751
MOD_NEK2_1	837	842	PF00069	0.409
MOD_NEK2_2	516	521	PF00069	0.430
MOD_NEK2_2	809	814	PF00069	0.502
MOD_OFUCOSY	534	539	PF10250	0.759
MOD_PIKK_1	164	170	PF00454	0.711
MOD_PIKK_1	697	703	PF00454	0.639
MOD_PIKK_1	736	742	PF00454	0.806
MOD_PKA_1	273	279	PF00069	0.439
MOD_PKA_2	112	118	PF00069	0.591
MOD_PKA_2	273	279	PF00069	0.439
MOD_PKA_2	404	410	PF00069	0.546
MOD_PKA_2	43	49	PF00069	0.725
MOD_PKA_2	564	570	PF00069	0.585
MOD_PKA_2	661	667	PF00069	0.478
MOD_PKA_2	707	713	PF00069	0.667
MOD_PKA_2	748	754	PF00069	0.524
MOD_PKA_2	783	789	PF00069	0.481
MOD_Plk_1	541	547	PF00069	0.505
MOD_Plk_1	574	580	PF00069	0.594
MOD_Plk_1	601	607	PF00069	0.303
MOD_Plk_1	809	815	PF00069	0.557
MOD_Plk_2-3	451	457	PF00069	0.430
MOD_Plk_4	256	262	PF00069	0.658
MOD_Plk_4	273	279	PF00069	0.524
MOD_Plk_4	346	352	PF00069	0.455
MOD_Plk_4	519	525	PF00069	0.569
MOD_Plk_4	574	580	PF00069	0.559
MOD_Plk_4	583	589	PF00069	0.441
MOD_Plk_4	77	83	PF00069	0.676
MOD_Plk_4	798	804	PF00069	0.364
MOD_Plk_4	837	843	PF00069	0.415
MOD_ProDKin_1	123	129	PF00069	0.760
MOD_ProDKin_1	27	33	PF00069	0.726
MOD_ProDKin_1	315	321	PF00069	0.578
MOD_ProDKin_1	375	381	PF00069	0.801
MOD_ProDKin_1	384	390	PF00069	0.672
MOD_ProDKin_1	396	402	PF00069	0.628
MOD_ProDKin_1	406	412	PF00069	0.745
MOD_ProDKin_1	526	532	PF00069	0.756
MOD_ProDKin_1	824	830	PF00069	0.489
MOD_SUMO_rev_2	480	488	PF00179	0.596
MOD_SUMO_rev_2	761	768	PF00179	0.731
TRG_DiLeu_BaEn_1	148	153	PF01217	0.569
TRG_DiLeu_BaEn_1	342	347	PF01217	0.453
TRG_DiLeu_BaLyEn_6	618	623	PF01217	0.586
TRG_ENDOCYTIC_2	23	26	PF00928	0.786
TRG_ENDOCYTIC_2	275	278	PF00928	0.361
TRG_ENDOCYTIC_2	306	309	PF00928	0.432
TRG_ENDOCYTIC_2	543	546	PF00928	0.511
TRG_ENDOCYTIC_2	584	587	PF00928	0.442
TRG_ENDOCYTIC_2	654	657	PF00928	0.476
TRG_ENDOCYTIC_2	85	88	PF00928	0.433
TRG_ER_diArg_1	272	274	PF00400	0.501
TRG_ER_diArg_1	4	6	PF00400	0.532
TRG_ER_diArg_1	439	441	PF00400	0.579
TRG_ER_diArg_1	620	622	PF00400	0.515
TRG_NES_CRM1_1	321	334	PF08389	0.498
TRG_Pf-PMV_PEXEL_1	621	626	PF00026	0.615
TRG_Pf-PMV_PEXEL_1	639	643	PF00026	0.431
TRG_Pf-PMV_PEXEL_1	766	770	PF00026	0.502
TRG_Pf-PMV_PEXEL_1	811	816	PF00026	0.504
TRG_Pf-PMV_PEXEL_1	97	101	PF00026	0.686

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P6M5	Leptomonas seymouri	35%	100%
A0A3S7WX62	Leishmania donovani	63%	98%
A4HZW6	Leishmania infantum	63%	98%
E9AVS4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	62%	100%
Q4QBQ5	Leishmania major	62%	100%