DNA primase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

DNA primase

Gene product:

DNA primase small subunit, putative

Species:

Leishmania braziliensis

UniProt:

A4HC88_LEIBR

TriTrypDb:

LbrM.22.0370 , LBRM2903_220008900

Length:

450

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0000428	DNA-directed RNA polymerase complex	4	12
GO:0030880	RNA polymerase complex	3	12
GO:0032991	protein-containing complex	1	12
GO:0061695	transferase complex, transferring phosphorus-containing groups	4	12
GO:0140535	intracellular protein-containing complex	2	12
GO:1902494	catalytic complex	2	12
GO:1990234	transferase complex	3	12
GO:0005654	nucleoplasm	2	1
GO:0005658	alpha DNA polymerase:primase complex	4	1
GO:0005737	cytoplasm	2	1
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	1
GO:0042575	DNA polymerase complex	3	1
GO:0055029	nuclear DNA-directed RNA polymerase complex	3	1
GO:0097014	ciliary plasm	5	1
GO:0099568	cytoplasmic region	3	1
GO:0110165	cellular anatomical entity	1	1
GO:0140513	nuclear protein-containing complex	2	1

Expansion

Sequence features

A4HC88

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HC88

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006269	DNA replication, synthesis of RNA primer	6	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0009058	biosynthetic process	2	1
GO:0009059	macromolecule biosynthetic process	4	1
GO:0009987	cellular process	1	1
GO:0016070	RNA metabolic process	5	1
GO:0018130	heterocycle biosynthetic process	4	1
GO:0019438	aromatic compound biosynthetic process	4	1
GO:0032774	RNA biosynthetic process	5	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0034654	nucleobase-containing compound biosynthetic process	4	1
GO:0043170	macromolecule metabolic process	3	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044249	cellular biosynthetic process	3	1
GO:0044271	cellular nitrogen compound biosynthetic process	4	1
GO:0046483	heterocycle metabolic process	3	1
GO:0071704	organic substance metabolic process	2	1
GO:0090304	nucleic acid metabolic process	4	1
GO:1901360	organic cyclic compound metabolic process	3	1
GO:1901362	organic cyclic compound biosynthetic process	4	1
GO:1901576	organic substance biosynthetic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0003896	DNA primase activity	7	12
GO:0003899	DNA-directed 5'-3' RNA polymerase activity	6	12
GO:0005488	binding	1	12
GO:0016740	transferase activity	2	12
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	12
GO:0016779	nucleotidyltransferase activity	4	12
GO:0034062	5'-3' RNA polymerase activity	5	12
GO:0043167	ion binding	2	12
GO:0043169	cation binding	3	12
GO:0046872	metal ion binding	4	12
GO:0097747	RNA polymerase activity	4	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	291	295	PF00656	0.450
CLV_C14_Caspase3-7	403	407	PF00656	0.482
CLV_C14_Caspase3-7	85	89	PF00656	0.708
CLV_NRD_NRD_1	101	103	PF00675	0.325
CLV_NRD_NRD_1	219	221	PF00675	0.296
CLV_NRD_NRD_1	229	231	PF00675	0.278
CLV_NRD_NRD_1	321	323	PF00675	0.304
CLV_NRD_NRD_1	378	380	PF00675	0.319
CLV_PCSK_KEX2_1	101	103	PF00082	0.325
CLV_PCSK_KEX2_1	219	221	PF00082	0.296
CLV_PCSK_KEX2_1	229	231	PF00082	0.278
CLV_PCSK_KEX2_1	321	323	PF00082	0.319
CLV_PCSK_KEX2_1	378	380	PF00082	0.319
CLV_PCSK_SKI1_1	210	214	PF00082	0.323
CLV_PCSK_SKI1_1	232	236	PF00082	0.363
CLV_PCSK_SKI1_1	239	243	PF00082	0.244
CLV_PCSK_SKI1_1	273	277	PF00082	0.374
CLV_PCSK_SKI1_1	282	286	PF00082	0.315
CLV_PCSK_SKI1_1	322	326	PF00082	0.467
CLV_PCSK_SKI1_1	436	440	PF00082	0.433
CLV_Separin_Metazoa	128	132	PF03568	0.477
DEG_APCC_DBOX_1	378	386	PF00400	0.303
DEG_APCC_DBOX_1	428	436	PF00400	0.406
DEG_Nend_UBRbox_3	1	3	PF02207	0.661
DEG_SPOP_SBC_1	289	293	PF00917	0.450
DOC_CDC14_PxL_1	365	373	PF14671	0.303
DOC_CKS1_1	37	42	PF01111	0.457
DOC_CYCLIN_RxL_1	158	168	PF00134	0.331
DOC_CYCLIN_yCln2_LP_2	383	389	PF00134	0.303
DOC_MAPK_DCC_7	429	437	PF00069	0.256
DOC_MAPK_gen_1	219	226	PF00069	0.303
DOC_MAPK_gen_1	378	385	PF00069	0.319
DOC_MAPK_HePTP_8	375	387	PF00069	0.319
DOC_MAPK_MEF2A_6	219	226	PF00069	0.303
DOC_MAPK_MEF2A_6	378	387	PF00069	0.304
DOC_MAPK_MEF2A_6	429	437	PF00069	0.325
DOC_MAPK_NFAT4_5	219	227	PF00069	0.303
DOC_MAPK_RevD_3	364	379	PF00069	0.319
DOC_PP1_RVXF_1	159	166	PF00149	0.328
DOC_PP2B_LxvP_1	383	386	PF13499	0.303
DOC_USP7_MATH_1	190	194	PF00917	0.388
DOC_USP7_MATH_1	348	352	PF00917	0.424
DOC_USP7_MATH_1	391	395	PF00917	0.275
DOC_WD40_RPTOR_TOS_1	165	171	PF00400	0.303
DOC_WW_Pin1_4	36	41	PF00397	0.502
LIG_14-3-3_CanoR_1	115	120	PF00244	0.352
LIG_14-3-3_CanoR_1	429	433	PF00244	0.462
LIG_14-3-3_CanoR_1	48	52	PF00244	0.624
LIG_Actin_WH2_1	239	257	PF00022	0.424
LIG_Actin_WH2_2	242	257	PF00022	0.424
LIG_CtBP_PxDLS_1	18	22	PF00389	0.291
LIG_deltaCOP1_diTrp_1	401	408	PF00928	0.396
LIG_EH1_1	359	367	PF00400	0.424
LIG_FHA_1	21	27	PF00498	0.367
LIG_FHA_1	238	244	PF00498	0.336
LIG_FHA_1	311	317	PF00498	0.418
LIG_FHA_1	354	360	PF00498	0.412
LIG_FHA_1	37	43	PF00498	0.537
LIG_FHA_1	391	397	PF00498	0.303
LIG_FHA_1	429	435	PF00498	0.467
LIG_FHA_1	61	67	PF00498	0.653
LIG_FHA_2	233	239	PF00498	0.377
LIG_FHA_2	276	282	PF00498	0.370
LIG_FHA_2	375	381	PF00498	0.295
LIG_LIR_Apic_2	427	433	PF02991	0.354
LIG_LIR_Gen_1	103	113	PF02991	0.353
LIG_LIR_Gen_1	11	21	PF02991	0.345
LIG_LIR_Gen_1	401	410	PF02991	0.392
LIG_LIR_Nem_3	103	109	PF02991	0.322
LIG_LIR_Nem_3	11	16	PF02991	0.345
LIG_LIR_Nem_3	111	116	PF02991	0.384
LIG_LIR_Nem_3	170	175	PF02991	0.319
LIG_LIR_Nem_3	206	212	PF02991	0.303
LIG_LIR_Nem_3	23	28	PF02991	0.319
LIG_LIR_Nem_3	281	287	PF02991	0.312
LIG_LIR_Nem_3	341	345	PF02991	0.290
LIG_LIR_Nem_3	401	405	PF02991	0.390
LIG_LYPXL_S_1	367	371	PF13949	0.303
LIG_LYPXL_yS_3	368	371	PF13949	0.303
LIG_MYND_1	33	37	PF01753	0.414
LIG_Pex14_2	284	288	PF04695	0.303
LIG_SH2_CRK	172	176	PF00017	0.315
LIG_SH2_GRB2like	172	175	PF00017	0.388
LIG_SH2_NCK_1	13	17	PF00017	0.470
LIG_SH2_PTP2	211	214	PF00017	0.338
LIG_SH2_SRC	13	16	PF00017	0.441
LIG_SH2_SRC	172	175	PF00017	0.388
LIG_SH2_STAP1	13	17	PF00017	0.365
LIG_SH2_STAT5	187	190	PF00017	0.338
LIG_SH2_STAT5	211	214	PF00017	0.303
LIG_SH2_STAT5	245	248	PF00017	0.319
LIG_SH2_STAT5	345	348	PF00017	0.323
LIG_SH2_STAT5	395	398	PF00017	0.198
LIG_SH2_STAT5	98	101	PF00017	0.336
LIG_SH3_3	245	251	PF00018	0.290
LIG_SH3_3	31	37	PF00018	0.615
LIG_SUMO_SIM_anti_2	313	318	PF11976	0.333
LIG_SUMO_SIM_par_1	17	23	PF11976	0.363
LIG_TRAF2_1	121	124	PF00917	0.478
LIG_TRAF2_1	351	354	PF00917	0.424
LIG_TYR_ITIM	243	248	PF00017	0.303
LIG_TYR_ITIM	366	371	PF00017	0.303
MOD_CK1_1	118	124	PF00069	0.353
MOD_CK1_1	177	183	PF00069	0.316
MOD_CK1_1	49	55	PF00069	0.651
MOD_CK2_1	118	124	PF00069	0.353
MOD_CK2_1	232	238	PF00069	0.385
MOD_CK2_1	348	354	PF00069	0.424
MOD_CK2_1	422	428	PF00069	0.428
MOD_CK2_1	5	11	PF00069	0.419
MOD_CK2_1	75	81	PF00069	0.492
MOD_Cter_Amidation	217	220	PF01082	0.303
MOD_Cter_Amidation	319	322	PF01082	0.424
MOD_GlcNHglycan	120	123	PF01048	0.370
MOD_GlcNHglycan	216	219	PF01048	0.303
MOD_GlcNHglycan	51	54	PF01048	0.639
MOD_GlcNHglycan	77	80	PF01048	0.653
MOD_GSK3_1	111	118	PF00069	0.367
MOD_GSK3_1	20	27	PF00069	0.377
MOD_GSK3_1	250	257	PF00069	0.367
MOD_NEK2_1	104	109	PF00069	0.321
MOD_NEK2_1	254	259	PF00069	0.424
MOD_NEK2_1	287	292	PF00069	0.331
MOD_NEK2_1	340	345	PF00069	0.273
MOD_OFUCOSY	188	194	PF10250	0.360
MOD_PIKK_1	20	26	PF00454	0.389
MOD_PIKK_1	275	281	PF00454	0.418
MOD_PKA_2	254	260	PF00069	0.424
MOD_PKA_2	428	434	PF00069	0.510
MOD_PKA_2	47	53	PF00069	0.488
MOD_PKB_1	230	238	PF00069	0.388
MOD_Plk_1	237	243	PF00069	0.308
MOD_Plk_1	310	316	PF00069	0.333
MOD_Plk_1	340	346	PF00069	0.273
MOD_Plk_1	46	52	PF00069	0.732
MOD_Plk_1	5	11	PF00069	0.408
MOD_Plk_1	60	66	PF00069	0.464
MOD_Plk_2-3	440	446	PF00069	0.511
MOD_Plk_4	198	204	PF00069	0.385
MOD_Plk_4	254	260	PF00069	0.326
MOD_Plk_4	335	341	PF00069	0.446
MOD_Plk_4	391	397	PF00069	0.314
MOD_ProDKin_1	36	42	PF00069	0.506
MOD_SUMO_for_1	44	47	PF00179	0.736
MOD_SUMO_rev_2	82	92	PF00179	0.629
TRG_AP2beta_CARGO_1	111	120	PF09066	0.485
TRG_DiLeu_BaEn_1	238	243	PF01217	0.338
TRG_DiLeu_BaLyEn_6	31	36	PF01217	0.611
TRG_ENDOCYTIC_2	13	16	PF00928	0.356
TRG_ENDOCYTIC_2	172	175	PF00928	0.319
TRG_ENDOCYTIC_2	245	248	PF00928	0.303
TRG_ENDOCYTIC_2	368	371	PF00928	0.303
TRG_ER_diArg_1	229	232	PF00400	0.390
TRG_Pf-PMV_PEXEL_1	161	166	PF00026	0.321
TRG_Pf-PMV_PEXEL_1	347	352	PF00026	0.292
TRG_Pf-PMV_PEXEL_1	424	428	PF00026	0.425
TRG_Pf-PMV_PEXEL_1	436	440	PF00026	0.364

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PDK6	Leptomonas seymouri	73%	100%
A0A0S4IXR0	Bodo saltans	50%	100%
A0A1X0NUV3	Trypanosomatidae	57%	100%
A0A3R7KRU9	Trypanosoma rangeli	56%	100%
A0A3S7WWZ8	Leishmania donovani	85%	100%
A4HZR7	Leishmania infantum	85%	100%
C9ZRJ5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	57%	100%
E9AVM3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	84%	100%
O14215	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	33%	99%
P10363	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	32%	100%
P20664	Mus musculus	35%	100%
P34471	Caenorhabditis elegans	34%	100%
P49642	Homo sapiens	34%	100%
Q24317	Drosophila melanogaster	34%	100%
Q25998	Plasmodium falciparum (isolate K1 / Thailand)	33%	100%
Q4QBV6	Leishmania major	85%	100%
Q5V554	Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809)	25%	100%
Q7KQM1	Plasmodium falciparum (isolate 3D7)	33%	100%
V5AVR1	Trypanosoma cruzi	56%	100%