ATP-dependent DNA helicase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

DNA repair and recombination helicase protein PIF6, putative

Species:

Leishmania braziliensis

UniProt:

A4HBX5_LEIBR

TriTrypDb:

LbrM.21.1390 , LBRM2903_210019300

Length:

788

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 15

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 12
NetGPI	no	yes: 0, no: 12

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	13
GO:0110165	cellular anatomical entity	1	13
GO:0005634	nucleus	5	1
GO:0005657	replication fork	2	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1

Expansion

Sequence features

A4HBX5

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HBX5

Function

Biological processes
Term	Name	Level	Count
GO:0000723	telomere maintenance	5	13
GO:0006139	nucleobase-containing compound metabolic process	3	13
GO:0006259	DNA metabolic process	4	13
GO:0006281	DNA repair	5	13
GO:0006310	DNA recombination	5	13
GO:0006725	cellular aromatic compound metabolic process	3	13
GO:0006807	nitrogen compound metabolic process	2	13
GO:0006950	response to stress	2	13
GO:0006974	DNA damage response	4	13
GO:0006996	organelle organization	4	13
GO:0008152	metabolic process	1	13
GO:0009987	cellular process	1	13
GO:0016043	cellular component organization	3	13
GO:0032200	telomere organization	6	13
GO:0033554	cellular response to stress	3	13
GO:0034641	cellular nitrogen compound metabolic process	3	13
GO:0043170	macromolecule metabolic process	3	13
GO:0044237	cellular metabolic process	2	13
GO:0044238	primary metabolic process	2	13
GO:0044260	obsolete cellular macromolecule metabolic process	3	13
GO:0046483	heterocycle metabolic process	3	13
GO:0050896	response to stimulus	1	13
GO:0051276	chromosome organization	5	13
GO:0051716	cellular response to stimulus	2	13
GO:0071704	organic substance metabolic process	2	13
GO:0071840	cellular component organization or biogenesis	2	13
GO:0090304	nucleic acid metabolic process	4	13
GO:1901360	organic cyclic compound metabolic process	3	13
GO:0006260	DNA replication	5	1
GO:0032392	DNA geometric change	7	1
GO:0032508	DNA duplex unwinding	8	1
GO:0071103	DNA conformation change	6	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	13
GO:0003678	DNA helicase activity	3	13
GO:0003824	catalytic activity	1	13
GO:0004386	helicase activity	2	13
GO:0005488	binding	1	13
GO:0005524	ATP binding	5	13
GO:0008094	ATP-dependent activity, acting on DNA	2	13
GO:0016462	pyrophosphatase activity	5	13
GO:0016787	hydrolase activity	2	13
GO:0016817	hydrolase activity, acting on acid anhydrides	3	13
GO:0016818	hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	4	13
GO:0016887	ATP hydrolysis activity	7	13
GO:0017076	purine nucleotide binding	4	13
GO:0017111	ribonucleoside triphosphate phosphatase activity	6	13
GO:0030554	adenyl nucleotide binding	5	13
GO:0032553	ribonucleotide binding	3	13
GO:0032555	purine ribonucleotide binding	4	13
GO:0032559	adenyl ribonucleotide binding	5	13
GO:0035639	purine ribonucleoside triphosphate binding	4	13
GO:0036094	small molecule binding	2	13
GO:0043167	ion binding	2	13
GO:0043168	anion binding	3	13
GO:0097159	organic cyclic compound binding	2	13
GO:0097367	carbohydrate derivative binding	2	13
GO:0140097	catalytic activity, acting on DNA	3	13
GO:0140640	catalytic activity, acting on a nucleic acid	2	13
GO:0140657	ATP-dependent activity	1	13
GO:1901265	nucleoside phosphate binding	3	13
GO:1901363	heterocyclic compound binding	2	13
GO:0000287	magnesium ion binding	5	1
GO:0043169	cation binding	3	1
GO:0046872	metal ion binding	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	231	235	PF00656	0.727
CLV_MEL_PAP_1	503	509	PF00089	0.533
CLV_NRD_NRD_1	133	135	PF00675	0.699
CLV_NRD_NRD_1	408	410	PF00675	0.367
CLV_NRD_NRD_1	483	485	PF00675	0.449
CLV_NRD_NRD_1	517	519	PF00675	0.431
CLV_NRD_NRD_1	634	636	PF00675	0.440
CLV_NRD_NRD_1	679	681	PF00675	0.470
CLV_PCSK_KEX2_1	408	410	PF00082	0.507
CLV_PCSK_KEX2_1	483	485	PF00082	0.449
CLV_PCSK_KEX2_1	517	519	PF00082	0.445
CLV_PCSK_KEX2_1	634	636	PF00082	0.542
CLV_PCSK_KEX2_1	93	95	PF00082	0.566
CLV_PCSK_PC1ET2_1	93	95	PF00082	0.612
CLV_PCSK_SKI1_1	295	299	PF00082	0.717
CLV_PCSK_SKI1_1	492	496	PF00082	0.512
CLV_PCSK_SKI1_1	659	663	PF00082	0.413
CLV_PCSK_SKI1_1	664	668	PF00082	0.410
CLV_PCSK_SKI1_1	7	11	PF00082	0.465
DOC_MAPK_DCC_7	599	608	PF00069	0.340
DOC_MAPK_gen_1	634	641	PF00069	0.473
DOC_MAPK_gen_1	678	687	PF00069	0.431
DOC_MAPK_gen_1	93	100	PF00069	0.517
DOC_MAPK_MEF2A_6	599	608	PF00069	0.340
DOC_MAPK_MEF2A_6	634	641	PF00069	0.363
DOC_PP1_RVXF_1	244	250	PF00149	0.584
DOC_PP2B_LxvP_1	431	434	PF13499	0.304
DOC_SPAK_OSR1_1	506	510	PF12202	0.520
DOC_USP7_MATH_1	130	134	PF00917	0.703
DOC_USP7_MATH_1	2	6	PF00917	0.514
DOC_USP7_MATH_1	219	223	PF00917	0.809
DOC_USP7_MATH_1	402	406	PF00917	0.443
DOC_USP7_MATH_1	578	582	PF00917	0.570
DOC_USP7_MATH_1	697	701	PF00917	0.499
DOC_USP7_MATH_1	711	715	PF00917	0.716
DOC_USP7_UBL2_3	119	123	PF12436	0.510
DOC_WW_Pin1_4	201	206	PF00397	0.595
DOC_WW_Pin1_4	485	490	PF00397	0.370
LIG_14-3-3_CanoR_1	246	250	PF00244	0.487
LIG_14-3-3_CanoR_1	295	303	PF00244	0.540
LIG_14-3-3_CanoR_1	318	327	PF00244	0.446
LIG_14-3-3_CanoR_1	397	401	PF00244	0.458
LIG_14-3-3_CanoR_1	408	418	PF00244	0.318
LIG_14-3-3_CanoR_1	539	548	PF00244	0.501
LIG_14-3-3_CanoR_1	607	615	PF00244	0.510
LIG_14-3-3_CanoR_1	629	633	PF00244	0.593
LIG_14-3-3_CanoR_1	84	88	PF00244	0.511
LIG_14-3-3_CanoR_1	94	99	PF00244	0.420
LIG_Actin_WH2_2	382	399	PF00022	0.333
LIG_Actin_WH2_2	550	568	PF00022	0.475
LIG_APCC_ABBA_1	22	27	PF00400	0.468
LIG_APCC_ABBAyCdc20_2	21	27	PF00400	0.476
LIG_BIR_II_1	1	5	PF00653	0.628
LIG_BIR_III_3	1	5	PF00653	0.474
LIG_BIR_III_4	749	753	PF00653	0.492
LIG_BRCT_BRCA1_1	382	386	PF00533	0.367
LIG_BRCT_BRCA1_1	533	537	PF00533	0.576
LIG_CtBP_PxDLS_1	759	763	PF00389	0.534
LIG_DLG_GKlike_1	154	161	PF00625	0.526
LIG_FHA_1	101	107	PF00498	0.368
LIG_FHA_1	275	281	PF00498	0.417
LIG_FHA_1	314	320	PF00498	0.568
LIG_FHA_1	486	492	PF00498	0.393
LIG_FHA_1	510	516	PF00498	0.377
LIG_FHA_1	562	568	PF00498	0.512
LIG_FHA_1	618	624	PF00498	0.481
LIG_FHA_1	718	724	PF00498	0.739
LIG_FHA_1	84	90	PF00498	0.443
LIG_FHA_2	100	106	PF00498	0.462
LIG_FHA_2	500	506	PF00498	0.312
LIG_FHA_2	608	614	PF00498	0.432
LIG_FHA_2	686	692	PF00498	0.427
LIG_GBD_Chelix_1	392	400	PF00786	0.210
LIG_LIR_Gen_1	383	393	PF02991	0.401
LIG_LIR_Gen_1	429	436	PF02991	0.304
LIG_LIR_Gen_1	455	463	PF02991	0.483
LIG_LIR_Gen_1	542	553	PF02991	0.520
LIG_LIR_Gen_1	65	75	PF02991	0.555
LIG_LIR_LC3C_4	587	592	PF02991	0.247
LIG_LIR_Nem_3	169	175	PF02991	0.770
LIG_LIR_Nem_3	383	389	PF02991	0.343
LIG_LIR_Nem_3	429	433	PF02991	0.304
LIG_LIR_Nem_3	455	461	PF02991	0.394
LIG_LIR_Nem_3	534	540	PF02991	0.409
LIG_LIR_Nem_3	542	548	PF02991	0.385
LIG_LIR_Nem_3	63	69	PF02991	0.410
LIG_LIR_Nem_3	705	709	PF02991	0.480
LIG_MAD2	394	402	PF02301	0.367
LIG_PDZ_Class_3	783	788	PF00595	0.506
LIG_SH2_CRK	545	549	PF00017	0.530
LIG_SH2_NCK_1	545	549	PF00017	0.530
LIG_SH2_PTP2	673	676	PF00017	0.352
LIG_SH2_STAP1	172	176	PF00017	0.533
LIG_SH2_STAT3	524	527	PF00017	0.394
LIG_SH2_STAT5	175	178	PF00017	0.730
LIG_SH2_STAT5	673	676	PF00017	0.352
LIG_SH3_3	202	208	PF00018	0.682
LIG_SH3_3	5	11	PF00018	0.553
LIG_SH3_3	554	560	PF00018	0.469
LIG_SH3_3	598	604	PF00018	0.483
LIG_SH3_3	773	779	PF00018	0.653
LIG_SUMO_SIM_anti_2	371	377	PF11976	0.367
LIG_SUMO_SIM_anti_2	691	696	PF11976	0.460
LIG_SUMO_SIM_par_1	371	377	PF11976	0.291
LIG_SUMO_SIM_par_1	423	429	PF11976	0.319
LIG_SUMO_SIM_par_1	570	576	PF11976	0.388
LIG_SUMO_SIM_par_1	758	764	PF11976	0.539
LIG_SUMO_SIM_par_1	85	90	PF11976	0.501
LIG_TRAF2_1	305	308	PF00917	0.480
LIG_TRAF2_1	440	443	PF00917	0.449
LIG_TRAF2_1	502	505	PF00917	0.559
LIG_UBA3_1	657	664	PF00899	0.266
MOD_CDK_SPxxK_3	485	492	PF00069	0.367
MOD_CK1_1	199	205	PF00069	0.727
MOD_CK1_1	213	219	PF00069	0.696
MOD_CK1_1	228	234	PF00069	0.717
MOD_CK1_1	235	241	PF00069	0.653
MOD_CK1_1	379	385	PF00069	0.432
MOD_CK1_1	403	409	PF00069	0.306
MOD_CK1_1	43	49	PF00069	0.439
MOD_CK1_1	555	561	PF00069	0.437
MOD_CK1_1	581	587	PF00069	0.377
MOD_CK1_1	710	716	PF00069	0.590
MOD_CK1_1	717	723	PF00069	0.580
MOD_CK1_1	768	774	PF00069	0.766
MOD_CK2_1	171	177	PF00069	0.737
MOD_CK2_1	499	505	PF00069	0.444
MOD_CK2_1	607	613	PF00069	0.440
MOD_CK2_1	662	668	PF00069	0.491
MOD_GlcNHglycan	15	18	PF01048	0.477
MOD_GlcNHglycan	161	164	PF01048	0.727
MOD_GlcNHglycan	197	201	PF01048	0.754
MOD_GlcNHglycan	268	271	PF01048	0.400
MOD_GlcNHglycan	402	405	PF01048	0.231
MOD_GlcNHglycan	45	48	PF01048	0.540
MOD_GlcNHglycan	709	712	PF01048	0.710
MOD_GlcNHglycan	713	716	PF01048	0.765
MOD_GlcNHglycan	732	735	PF01048	0.638
MOD_GlcNHglycan	776	779	PF01048	0.686
MOD_GSK3_1	195	202	PF00069	0.696
MOD_GSK3_1	210	217	PF00069	0.637
MOD_GSK3_1	219	226	PF00069	0.650
MOD_GSK3_1	228	235	PF00069	0.718
MOD_GSK3_1	376	383	PF00069	0.482
MOD_GSK3_1	396	403	PF00069	0.162
MOD_GSK3_1	457	464	PF00069	0.424
MOD_GSK3_1	707	714	PF00069	0.655
MOD_GSK3_1	765	772	PF00069	0.582
MOD_GSK3_1	83	90	PF00069	0.559
MOD_N-GLC_1	186	191	PF02516	0.799
MOD_N-GLC_1	578	583	PF02516	0.431
MOD_NEK2_1	13	18	PF00069	0.505
MOD_NEK2_1	380	385	PF00069	0.478
MOD_NEK2_1	396	401	PF00069	0.364
MOD_NEK2_1	448	453	PF00069	0.210
MOD_NEK2_1	573	578	PF00069	0.423
MOD_NEK2_1	630	635	PF00069	0.523
MOD_NEK2_1	662	667	PF00069	0.532
MOD_NEK2_1	685	690	PF00069	0.497
MOD_NEK2_1	709	714	PF00069	0.577
MOD_NEK2_1	730	735	PF00069	0.462
MOD_NEK2_1	99	104	PF00069	0.352
MOD_NEK2_2	2	7	PF00069	0.491
MOD_NEK2_2	20	25	PF00069	0.426
MOD_NEK2_2	333	338	PF00069	0.304
MOD_NEK2_2	561	566	PF00069	0.329
MOD_NEK2_2	643	648	PF00069	0.359
MOD_PIKK_1	211	217	PF00454	0.750
MOD_PIKK_1	403	409	PF00454	0.359
MOD_PK_1	94	100	PF00069	0.552
MOD_PKA_2	195	201	PF00069	0.533
MOD_PKA_2	220	226	PF00069	0.808
MOD_PKA_2	245	251	PF00069	0.562
MOD_PKA_2	396	402	PF00069	0.333
MOD_PKA_2	43	49	PF00069	0.419
MOD_PKA_2	54	60	PF00069	0.462
MOD_PKA_2	606	612	PF00069	0.429
MOD_PKA_2	628	634	PF00069	0.602
MOD_PKA_2	83	89	PF00069	0.497
MOD_Plk_1	376	382	PF00069	0.387
MOD_Plk_1	509	515	PF00069	0.510
MOD_Plk_1	555	561	PF00069	0.458
MOD_Plk_1	578	584	PF00069	0.462
MOD_Plk_1	662	668	PF00069	0.533
MOD_Plk_1	683	689	PF00069	0.498
MOD_Plk_2-3	171	177	PF00069	0.689
MOD_Plk_4	171	177	PF00069	0.534
MOD_Plk_4	457	463	PF00069	0.210
MOD_Plk_4	509	515	PF00069	0.386
MOD_Plk_4	62	68	PF00069	0.421
MOD_Plk_4	662	668	PF00069	0.526
MOD_Plk_4	83	89	PF00069	0.435
MOD_Plk_4	94	100	PF00069	0.448
MOD_ProDKin_1	201	207	PF00069	0.595
MOD_ProDKin_1	485	491	PF00069	0.364
MOD_SUMO_for_1	297	300	PF00179	0.469
MOD_SUMO_for_1	783	786	PF00179	0.747
TRG_DiLeu_BaEn_1	510	515	PF01217	0.418
TRG_DiLeu_BaLyEn_6	327	332	PF01217	0.443
TRG_ENDOCYTIC_2	545	548	PF00928	0.473
TRG_ENDOCYTIC_2	673	676	PF00928	0.377
TRG_ER_diArg_1	289	292	PF00400	0.527
TRG_ER_diArg_1	482	484	PF00400	0.450
TRG_NES_CRM1_1	651	663	PF08389	0.546
TRG_Pf-PMV_PEXEL_1	501	505	PF00026	0.449

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0S4JCS0	Bodo saltans	41%	100%
A0A1X0NZA2	Trypanosomatidae	39%	92%
A0A3Q8IBY0	Leishmania donovani	80%	100%
A0A3R7MKN4	Trypanosoma rangeli	30%	82%
A0A422NAK1	Trypanosoma rangeli	49%	100%
A4HZ95	Leishmania infantum	80%	100%
C9ZUJ0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	29%	79%
D0A177	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	45%	99%
E9AVA3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	79%	100%
Q38CE9	Trypanosoma brucei brucei (strain 927/4 GUTat10.1)	45%	99%
Q4QC77	Leishmania major	81%	100%
Q57YG0	Trypanosoma brucei brucei (strain 927/4 GUTat10.1)	29%	79%
Q9UUA2	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	30%	98%
V5B8C2	Trypanosoma cruzi	30%	81%
V5BE68	Trypanosoma cruzi	48%	100%