tRNA N(3)-methylcytidine methyltransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

methyltransferase domain containing protein, putative

Species:

Leishmania braziliensis

UniProt:

A4HBJ6_LEIBR

TriTrypDb:

LbrM.21.0030 , LBRM2903_210005000

Length:

346

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Could not find GO cellular_component term for this entry.

Expansion

Sequence features

A4HBJ6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HBJ6

Function

Biological processes
Term	Name	Level	Count
GO:0001510	RNA methylation	4	12
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009451	RNA modification	5	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0032259	methylation	2	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043412	macromolecule modification	4	12
GO:0043414	macromolecule methylation	3	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044260	obsolete cellular macromolecule metabolic process	3	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:0006396	RNA processing	6	4
GO:0006399	tRNA metabolic process	7	4
GO:0006400	tRNA modification	6	4
GO:0008033	tRNA processing	8	4
GO:0030488	tRNA methylation	5	4
GO:0034470	ncRNA processing	7	4
GO:0034660	ncRNA metabolic process	6	4

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0008168	methyltransferase activity	4	12
GO:0016740	transferase activity	2	12
GO:0016741	transferase activity, transferring one-carbon groups	3	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	269	273	PF00656	0.410
CLV_NRD_NRD_1	324	326	PF00675	0.416
CLV_PCSK_KEX2_1	190	192	PF00082	0.385
CLV_PCSK_KEX2_1	243	245	PF00082	0.429
CLV_PCSK_KEX2_1	329	331	PF00082	0.332
CLV_PCSK_PC1ET2_1	190	192	PF00082	0.459
CLV_PCSK_PC1ET2_1	243	245	PF00082	0.230
CLV_PCSK_PC1ET2_1	329	331	PF00082	0.347
CLV_PCSK_PC7_1	325	331	PF00082	0.371
CLV_PCSK_SKI1_1	101	105	PF00082	0.257
CLV_PCSK_SKI1_1	167	171	PF00082	0.276
CLV_PCSK_SKI1_1	22	26	PF00082	0.421
CLV_PCSK_SKI1_1	240	244	PF00082	0.351
DEG_COP1_1	181	189	PF00400	0.186
DEG_Nend_UBRbox_2	1	3	PF02207	0.632
DEG_SPOP_SBC_1	62	66	PF00917	0.353
DOC_MAPK_gen_1	190	200	PF00069	0.261
DOC_MAPK_gen_1	213	222	PF00069	0.307
DOC_MAPK_gen_1	243	254	PF00069	0.439
DOC_MAPK_gen_1	325	336	PF00069	0.376
DOC_MAPK_MEF2A_6	193	202	PF00069	0.186
DOC_MAPK_MEF2A_6	216	224	PF00069	0.415
DOC_USP7_MATH_1	126	130	PF00917	0.435
DOC_USP7_MATH_1	172	176	PF00917	0.356
DOC_USP7_MATH_1	208	212	PF00917	0.431
DOC_USP7_MATH_1	43	47	PF00917	0.414
DOC_USP7_UBL2_3	243	247	PF12436	0.424
DOC_USP7_UBL2_3	329	333	PF12436	0.363
DOC_USP7_UBL2_3	50	54	PF12436	0.344
DOC_WW_Pin1_4	141	146	PF00397	0.329
LIG_14-3-3_CanoR_1	191	200	PF00244	0.424
LIG_14-3-3_CanoR_1	61	71	PF00244	0.527
LIG_Actin_WH2_2	228	245	PF00022	0.372
LIG_Actin_WH2_2	86	103	PF00022	0.448
LIG_APCC_ABBA_1	251	256	PF00400	0.338
LIG_BRCT_BRCA1_1	301	305	PF00533	0.186
LIG_FHA_1	168	174	PF00498	0.287
LIG_FHA_1	3	9	PF00498	0.747
LIG_FHA_2	305	311	PF00498	0.389
LIG_FHA_2	39	45	PF00498	0.553
LIG_LIR_Apic_2	256	260	PF02991	0.317
LIG_LIR_Apic_2	83	88	PF02991	0.320
LIG_LIR_Gen_1	214	225	PF02991	0.291
LIG_LIR_Gen_1	302	312	PF02991	0.186
LIG_LIR_Nem_3	214	220	PF02991	0.291
LIG_LIR_Nem_3	261	267	PF02991	0.397
LIG_LIR_Nem_3	302	308	PF02991	0.415
LIG_NBox_RRM_1	169	179	PF00076	0.186
LIG_Pex14_1	76	80	PF04695	0.384
LIG_Pex14_2	217	221	PF04695	0.319
LIG_Pex14_2	291	295	PF04695	0.320
LIG_PTB_Apo_2	334	341	PF02174	0.330
LIG_REV1ctd_RIR_1	262	270	PF16727	0.376
LIG_REV1ctd_RIR_1	288	297	PF16727	0.395
LIG_SH2_CRK	85	89	PF00017	0.300
LIG_SH2_NCK_1	257	261	PF00017	0.319
LIG_SH2_STAP1	20	24	PF00017	0.308
LIG_SH2_STAT5	160	163	PF00017	0.424
LIG_SH2_STAT5	178	181	PF00017	0.245
LIG_SH2_STAT5	92	95	PF00017	0.344
LIG_SH3_3	242	248	PF00018	0.260
LIG_SH3_3	69	75	PF00018	0.582
LIG_SUMO_SIM_par_1	169	175	PF11976	0.383
LIG_TRAF2_1	67	70	PF00917	0.580
MOD_CK1_1	129	135	PF00069	0.427
MOD_CK1_1	146	152	PF00069	0.365
MOD_CK1_1	64	70	PF00069	0.534
MOD_CK2_1	38	44	PF00069	0.456
MOD_CK2_1	63	69	PF00069	0.586
MOD_Cter_Amidation	327	330	PF01082	0.434
MOD_GlcNHglycan	145	148	PF01048	0.348
MOD_GlcNHglycan	174	177	PF01048	0.368
MOD_GlcNHglycan	226	229	PF01048	0.383
MOD_GlcNHglycan	236	239	PF01048	0.389
MOD_GlcNHglycan	80	83	PF01048	0.539
MOD_GSK3_1	14	21	PF00069	0.523
MOD_GSK3_1	146	153	PF00069	0.376
MOD_GSK3_1	181	188	PF00069	0.345
MOD_GSK3_1	224	231	PF00069	0.315
MOD_GSK3_1	57	64	PF00069	0.526
MOD_N-GLC_1	336	341	PF02516	0.364
MOD_NEK2_1	224	229	PF00069	0.304
MOD_NEK2_1	299	304	PF00069	0.367
MOD_NEK2_1	80	85	PF00069	0.547
MOD_NEK2_1	99	104	PF00069	0.413
MOD_NEK2_2	208	213	PF00069	0.186
MOD_PIKK_1	284	290	PF00454	0.424
MOD_PKA_2	18	24	PF00069	0.570
MOD_Plk_1	208	214	PF00069	0.289
MOD_Plk_1	43	49	PF00069	0.467
MOD_Plk_1	57	63	PF00069	0.299
MOD_Plk_4	120	126	PF00069	0.313
MOD_Plk_4	181	187	PF00069	0.334
MOD_ProDKin_1	141	147	PF00069	0.329
MOD_SUMO_rev_2	209	217	PF00179	0.448
MOD_SUMO_rev_2	21	26	PF00179	0.462
MOD_SUMO_rev_2	30	38	PF00179	0.425
TRG_DiLeu_BaEn_2	275	281	PF01217	0.186
TRG_DiLeu_BaLyEn_6	95	100	PF01217	0.365
TRG_NLS_MonoCore_2	189	194	PF00514	0.186
TRG_NLS_MonoExtC_3	189	195	PF00514	0.405
TRG_NLS_MonoExtN_4	187	194	PF00514	0.405
TRG_Pf-PMV_PEXEL_1	244	249	PF00026	0.347
TRG_Pf-PMV_PEXEL_1	45	49	PF00026	0.446
TRG_Pf-PMV_PEXEL_1	54	58	PF00026	0.421

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I1R8	Leptomonas seymouri	64%	99%
A0A0S4ISJ6	Bodo saltans	32%	78%
A0A1X0NXB7	Trypanosomatidae	43%	99%
A0A1X0P5J2	Trypanosomatidae	27%	100%
A0A3Q8IDT0	Leishmania donovani	72%	100%
A0A3R7M2M3	Trypanosoma rangeli	29%	100%
A0A3S7X9Q3	Leishmania donovani	27%	89%
A0A422MZQ7	Trypanosoma rangeli	44%	100%
A4HN30	Leishmania braziliensis	24%	100%
A4HZL8	Leishmania infantum	71%	100%
A4IBP9	Leishmania infantum	27%	89%
C9ZYX5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	26%	100%
D0A1J3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	43%	100%
E9AFI4	Leishmania major	25%	100%
E9AUW5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	71%	100%
E9B6N9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	26%	93%
O74386	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	30%	100%
Q0P5B2	Bos taurus	29%	92%
Q4QCL7	Leishmania major	72%	100%
Q5RDV8	Pongo abelii	27%	100%
Q6AXU8	Rattus norvegicus	28%	100%
Q86BS6	Drosophila melanogaster	24%	100%
Q8BVH9	Mus musculus	28%	100%
Q8T199	Dictyostelium discoideum	28%	79%
Q8TCB7	Homo sapiens	27%	100%
Q9P7L6	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	27%	100%
V5BCV4	Trypanosoma cruzi	30%	100%
V5DGW7	Trypanosoma cruzi	43%	100%