Putative 1,2-Dihydroxy-3-keto-5-methylthiopentene dioxygenase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

1,2-Dihydroxy-3-keto-5-methylthiopentene dioxygenase, putative

Species:

Leishmania braziliensis

UniProt:

A4HBG6_LEIBR

TriTrypDb:

LbrM.20.4060 , LBRM2903_200055000

Length:

280

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 3
Silverman et al.	no	yes: 3
Pissara et al.	yes	yes: 15

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005930	axoneme	2	1
GO:0110165	cellular anatomical entity	1	2
GO:0005634	nucleus	5	1
GO:0005737	cytoplasm	2	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1

Expansion

Sequence features

A4HBG6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HBG6

Function

Biological processes
Term	Name	Level	Count
GO:0000096	sulfur amino acid metabolic process	4	12
GO:0000097	sulfur amino acid biosynthetic process	5	12
GO:0006082	organic acid metabolic process	3	12
GO:0006520	amino acid metabolic process	3	12
GO:0006555	methionine metabolic process	5	12
GO:0006790	sulfur compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0008652	amino acid biosynthetic process	4	12
GO:0009058	biosynthetic process	2	12
GO:0009066	aspartate family amino acid metabolic process	5	12
GO:0009067	aspartate family amino acid biosynthetic process	6	12
GO:0009086	methionine biosynthetic process	6	12
GO:0009987	cellular process	1	12
GO:0016053	organic acid biosynthetic process	4	12
GO:0019752	carboxylic acid metabolic process	5	12
GO:0043436	oxoacid metabolic process	4	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044249	cellular biosynthetic process	3	12
GO:0044272	sulfur compound biosynthetic process	4	12
GO:0044281	small molecule metabolic process	2	12
GO:0044283	small molecule biosynthetic process	3	12
GO:0046394	carboxylic acid biosynthetic process	5	12
GO:0071704	organic substance metabolic process	2	12
GO:1901564	organonitrogen compound metabolic process	3	12
GO:1901566	organonitrogen compound biosynthetic process	4	12
GO:1901576	organic substance biosynthetic process	3	12
GO:1901605	alpha-amino acid metabolic process	4	12
GO:1901607	alpha-amino acid biosynthetic process	5	12
GO:0019509	L-methionine salvage from methylthioadenosine	6	1
GO:0043094	cellular metabolic compound salvage	3	1
GO:0043102	amino acid salvage	4	1
GO:0071265	L-methionine biosynthetic process	7	1
GO:0071267	L-methionine salvage	5	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0010309	acireductone dioxygenase [iron(II)-requiring] activity	5	12
GO:0016491	oxidoreductase activity	2	12
GO:0016701	oxidoreductase activity, acting on single donors with incorporation of molecular oxygen	3	12
GO:0016702	oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen	4	12
GO:0051213	dioxygenase activity	3	12
GO:0010308	acireductone dioxygenase (Ni2+-requiring) activity	5	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	252	256	PF00656	0.480
CLV_NRD_NRD_1	112	114	PF00675	0.497
CLV_NRD_NRD_1	20	22	PF00675	0.505
CLV_NRD_NRD_1	32	34	PF00675	0.466
CLV_NRD_NRD_1	42	44	PF00675	0.468
CLV_NRD_NRD_1	45	47	PF00675	0.472
CLV_NRD_NRD_1	57	59	PF00675	0.440
CLV_PCSK_FUR_1	43	47	PF00082	0.567
CLV_PCSK_FUR_1	58	62	PF00082	0.481
CLV_PCSK_KEX2_1	22	24	PF00082	0.490
CLV_PCSK_KEX2_1	248	250	PF00082	0.630
CLV_PCSK_KEX2_1	32	34	PF00082	0.490
CLV_PCSK_KEX2_1	44	46	PF00082	0.469
CLV_PCSK_KEX2_1	57	59	PF00082	0.440
CLV_PCSK_KEX2_1	60	62	PF00082	0.433
CLV_PCSK_KEX2_1	67	69	PF00082	0.552
CLV_PCSK_PC1ET2_1	22	24	PF00082	0.490
CLV_PCSK_PC1ET2_1	248	250	PF00082	0.630
CLV_PCSK_PC1ET2_1	44	46	PF00082	0.549
CLV_PCSK_PC1ET2_1	57	59	PF00082	0.432
CLV_PCSK_PC1ET2_1	60	62	PF00082	0.420
CLV_PCSK_PC1ET2_1	67	69	PF00082	0.557
CLV_PCSK_SKI1_1	32	36	PF00082	0.574
DEG_APCC_DBOX_1	257	265	PF00400	0.496
DEG_Nend_UBRbox_2	1	3	PF02207	0.619
DOC_MAPK_gen_1	123	133	PF00069	0.512
DOC_MAPK_gen_1	150	160	PF00069	0.461
DOC_MAPK_gen_1	197	206	PF00069	0.471
DOC_MAPK_gen_1	258	267	PF00069	0.359
DOC_MAPK_gen_1	43	53	PF00069	0.539
DOC_MAPK_HePTP_8	120	132	PF00069	0.512
DOC_MAPK_HePTP_8	196	208	PF00069	0.560
DOC_MAPK_MEF2A_6	113	122	PF00069	0.470
DOC_MAPK_MEF2A_6	123	132	PF00069	0.469
DOC_MAPK_MEF2A_6	199	208	PF00069	0.560
DOC_USP7_UBL2_3	110	114	PF12436	0.438
DOC_USP7_UBL2_3	144	148	PF12436	0.504
LIG_14-3-3_CanoR_1	113	118	PF00244	0.395
LIG_14-3-3_CanoR_1	134	143	PF00244	0.512
LIG_14-3-3_CanoR_1	223	227	PF00244	0.566
LIG_EVH1_1	77	81	PF00568	0.490
LIG_FHA_1	104	110	PF00498	0.464
LIG_FHA_1	153	159	PF00498	0.458
LIG_FHA_2	161	167	PF00498	0.467
LIG_FHA_2	177	183	PF00498	0.458
LIG_FHA_2	223	229	PF00498	0.458
LIG_LIR_Gen_1	138	147	PF02991	0.464
LIG_LIR_Gen_1	201	210	PF02991	0.490
LIG_LIR_Gen_1	228	238	PF02991	0.458
LIG_LIR_LC3C_4	212	215	PF02991	0.512
LIG_LIR_Nem_3	138	143	PF02991	0.499
LIG_LIR_Nem_3	181	187	PF02991	0.514
LIG_LIR_Nem_3	191	196	PF02991	0.450
LIG_LIR_Nem_3	201	206	PF02991	0.494
LIG_LIR_Nem_3	228	234	PF02991	0.471
LIG_LIR_Nem_3	63	69	PF02991	0.515
LIG_Pex14_2	169	173	PF04695	0.458
LIG_SH2_PTP2	231	234	PF00017	0.500
LIG_SH2_SRC	174	177	PF00017	0.560
LIG_SH2_SRC	193	196	PF00017	0.366
LIG_SH2_STAP1	30	34	PF00017	0.574
LIG_SH2_STAT5	178	181	PF00017	0.537
LIG_SH2_STAT5	193	196	PF00017	0.411
LIG_SH2_STAT5	231	234	PF00017	0.499
LIG_SH2_STAT5	263	266	PF00017	0.378
LIG_SH2_STAT5	94	97	PF00017	0.404
LIG_SH3_3	112	118	PF00018	0.537
LIG_SH3_3	211	217	PF00018	0.528
LIG_SH3_3	75	81	PF00018	0.507
LIG_SH3_4	110	117	PF00018	0.577
LIG_SUMO_SIM_anti_2	212	217	PF11976	0.512
LIG_UBA3_1	264	269	PF00899	0.447
MOD_CK1_1	105	111	PF00069	0.486
MOD_CK1_1	138	144	PF00069	0.478
MOD_CK2_1	165	171	PF00069	0.543
MOD_CK2_1	176	182	PF00069	0.496
MOD_CK2_1	222	228	PF00069	0.458
MOD_Cter_Amidation	111	114	PF01082	0.416
MOD_GlcNHglycan	115	118	PF01048	0.485
MOD_GSK3_1	249	256	PF00069	0.550
MOD_NEK2_1	133	138	PF00069	0.525
MOD_NEK2_1	188	193	PF00069	0.570
MOD_NEK2_2	118	123	PF00069	0.458
MOD_NEK2_2	263	268	PF00069	0.441
MOD_PIKK_1	158	164	PF00454	0.458
MOD_PIKK_1	271	277	PF00454	0.551
MOD_PIKK_1	97	103	PF00454	0.508
MOD_PKA_1	113	119	PF00069	0.478
MOD_PKA_1	32	38	PF00069	0.432
MOD_PKA_2	133	139	PF00069	0.512
MOD_PKA_2	222	228	PF00069	0.466
MOD_PKA_2	32	38	PF00069	0.578
MOD_Plk_1	138	144	PF00069	0.448
MOD_Plk_1	165	171	PF00069	0.560
MOD_Plk_2-3	176	182	PF00069	0.560
MOD_Plk_2-3	2	8	PF00069	0.664
MOD_Plk_4	165	171	PF00069	0.563
MOD_Plk_4	188	194	PF00069	0.546
MOD_SUMO_rev_2	149	155	PF00179	0.536
MOD_SUMO_rev_2	156	161	PF00179	0.511
MOD_SUMO_rev_2	63	69	PF00179	0.605
TRG_DiLeu_BaEn_4	64	70	PF01217	0.495
TRG_ENDOCYTIC_2	193	196	PF00928	0.439
TRG_ENDOCYTIC_2	231	234	PF00928	0.476
TRG_ENDOCYTIC_2	235	238	PF00928	0.477
TRG_ER_diArg_1	258	261	PF00400	0.500
TRG_ER_diArg_1	32	34	PF00400	0.491
TRG_ER_diArg_1	43	46	PF00400	0.441
TRG_NLS_Bipartite_1	43	62	PF00514	0.612
TRG_NLS_MonoCore_2	57	62	PF00514	0.621
TRG_NLS_MonoExtC_3	57	63	PF00514	0.622
TRG_NLS_MonoExtN_4	57	62	PF00514	0.621
TRG_Pf-PMV_PEXEL_1	123	127	PF00026	0.360
TRG_Pf-PMV_PEXEL_1	32	36	PF00026	0.520

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PA36	Leptomonas seymouri	78%	100%
A0A0S4IQB1	Bodo saltans	58%	98%
A0A1X0NWZ5	Trypanosomatidae	29%	100%
A0A1X0PAJ9	Trypanosomatidae	67%	99%
A0A3S7X8L3	Leishmania donovani	93%	100%
A0A422NJ26	Trypanosoma rangeli	60%	99%
A1L4T4	Oryza sativa subsp. japonica	30%	100%
A2Z7C4	Oryza sativa subsp. indica	30%	100%
A4IAL2	Leishmania infantum	92%	100%
A9SCJ6	Physcomitrium patens	34%	100%
C9ZLM6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	65%	100%
E9B5N7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	93%	100%
F6HDT7	Vitis vinifera	31%	100%
Q4Q281	Leishmania major	93%	100%
Q8W108	Arabidopsis thaliana	29%	100%
V5B654	Trypanosoma cruzi	63%	99%