Defective in cullin neddylation protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Cullin binding, putative

Species:

Leishmania braziliensis

UniProt:

A4HBF0_LEIBR

TriTrypDb:

LbrM.20.3900 , LBRM2903_200053300

Length:

244

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0000151	ubiquitin ligase complex	3	1
GO:0032991	protein-containing complex	1	1
GO:0140535	intracellular protein-containing complex	2	1
GO:1902494	catalytic complex	2	1
GO:1990234	transferase complex	3	1
GO:0016020	membrane	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4HBF0

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HBF0

Function

Biological processes
Term	Name	Level	Count
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0009893	positive regulation of metabolic process	4	1
GO:0010604	positive regulation of macromolecule metabolic process	5	1
GO:0019222	regulation of metabolic process	3	1
GO:0019538	protein metabolic process	3	1
GO:0031396	regulation of protein ubiquitination	8	1
GO:0031398	positive regulation of protein ubiquitination	9	1
GO:0031399	regulation of protein modification process	6	1
GO:0031401	positive regulation of protein modification process	7	1
GO:0032446	protein modification by small protein conjugation	6	1
GO:0036211	protein modification process	4	1
GO:0043085	positive regulation of catalytic activity	4	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0044093	positive regulation of molecular function	3	1
GO:0044238	primary metabolic process	2	1
GO:0045116	protein neddylation	7	1
GO:0048518	positive regulation of biological process	3	1
GO:0050789	regulation of biological process	2	1
GO:0050790	regulation of catalytic activity	3	1
GO:0051171	regulation of nitrogen compound metabolic process	4	1
GO:0051173	positive regulation of nitrogen compound metabolic process	5	1
GO:0051246	regulation of protein metabolic process	5	1
GO:0051247	positive regulation of protein metabolic process	6	1
GO:0051338	regulation of transferase activity	4	1
GO:0051347	positive regulation of transferase activity	5	1
GO:0051438	regulation of ubiquitin-protein transferase activity	5	1
GO:0051443	positive regulation of ubiquitin-protein transferase activity	6	1
GO:0060255	regulation of macromolecule metabolic process	4	1
GO:0065007	biological regulation	1	1
GO:0065009	regulation of molecular function	2	1
GO:0070647	protein modification by small protein conjugation or removal	5	1
GO:0071704	organic substance metabolic process	2	1
GO:0080090	regulation of primary metabolic process	4	1
GO:1901564	organonitrogen compound metabolic process	3	1
GO:1903320	regulation of protein modification by small protein conjugation or removal	7	1
GO:1903322	positive regulation of protein modification by small protein conjugation or removal	8	1

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	1
GO:0005515	protein binding	2	1
GO:0019899	enzyme binding	3	1
GO:0031624	ubiquitin conjugating enzyme binding	5	1
GO:0032182	ubiquitin-like protein binding	3	1
GO:0044390	ubiquitin-like protein conjugating enzyme binding	4	1
GO:0097602	cullin family protein binding	3	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	161	163	PF00675	0.453
CLV_PCSK_KEX2_1	161	163	PF00082	0.453
CLV_PCSK_KEX2_1	180	182	PF00082	0.139
CLV_PCSK_PC1ET2_1	180	182	PF00082	0.201
CLV_PCSK_SKI1_1	161	165	PF00082	0.378
CLV_PCSK_SKI1_1	181	185	PF00082	0.123
DEG_Nend_UBRbox_1	1	4	PF02207	0.737
DOC_MAPK_gen_1	45	55	PF00069	0.659
DOC_MAPK_gen_1	6	13	PF00069	0.691
DOC_MAPK_MEF2A_6	203	211	PF00069	0.298
DOC_MAPK_MEF2A_6	36	43	PF00069	0.684
DOC_MAPK_MEF2A_6	48	55	PF00069	0.650
DOC_MAPK_NFAT4_5	48	56	PF00069	0.656
DOC_USP7_MATH_1	215	219	PF00917	0.458
DOC_USP7_MATH_1	9	13	PF00917	0.689
DOC_USP7_UBL2_3	180	184	PF12436	0.416
LIG_14-3-3_CanoR_1	162	172	PF00244	0.425
LIG_14-3-3_CanoR_1	6	12	PF00244	0.651
LIG_BRCT_BRCA1_1	140	144	PF00533	0.345
LIG_deltaCOP1_diTrp_1	200	208	PF00928	0.306
LIG_FHA_1	164	170	PF00498	0.468
LIG_FHA_1	185	191	PF00498	0.244
LIG_FHA_1	192	198	PF00498	0.201
LIG_FHA_1	236	242	PF00498	0.633
LIG_FHA_1	38	44	PF00498	0.693
LIG_FHA_2	105	111	PF00498	0.571
LIG_FHA_2	63	69	PF00498	0.613
LIG_LIR_Gen_1	141	152	PF02991	0.274
LIG_LIR_Gen_1	52	61	PF02991	0.566
LIG_LIR_Nem_3	149	155	PF02991	0.251
LIG_LIR_Nem_3	200	205	PF02991	0.302
LIG_LIR_Nem_3	52	58	PF02991	0.547
LIG_Pex14_1	172	176	PF04695	0.322
LIG_SH2_CRK	152	156	PF00017	0.298
LIG_SH2_GRB2like	116	119	PF00017	0.414
LIG_SH2_SRC	116	119	PF00017	0.667
LIG_SH2_SRC	152	155	PF00017	0.354
LIG_SH2_STAP1	148	152	PF00017	0.276
LIG_SH2_STAT5	148	151	PF00017	0.383
LIG_SH2_STAT5	154	157	PF00017	0.344
LIG_SH3_3	27	33	PF00018	0.736
LIG_SUMO_SIM_anti_2	10	16	PF11976	0.472
LIG_SUMO_SIM_par_1	218	227	PF11976	0.201
MOD_CK1_1	20	26	PF00069	0.562
MOD_CK1_1	227	233	PF00069	0.201
MOD_CK1_1	46	52	PF00069	0.654
MOD_CK1_1	64	70	PF00069	0.691
MOD_CK2_1	104	110	PF00069	0.342
MOD_CK2_1	46	52	PF00069	0.551
MOD_CK2_1	84	90	PF00069	0.644
MOD_CMANNOS	185	188	PF00535	0.306
MOD_GlcNHglycan	139	143	PF01048	0.371
MOD_GlcNHglycan	229	232	PF01048	0.353
MOD_GlcNHglycan	45	48	PF01048	0.689
MOD_GlcNHglycan	70	73	PF01048	0.657
MOD_GSK3_1	191	198	PF00069	0.320
MOD_GSK3_1	37	44	PF00069	0.514
MOD_GSK3_1	62	69	PF00069	0.640
MOD_GSK3_1	80	87	PF00069	0.527
MOD_N-GLC_1	17	22	PF02516	0.584
MOD_N-GLC_1	191	196	PF02516	0.295
MOD_NEK2_1	146	151	PF00069	0.239
MOD_NEK2_1	163	168	PF00069	0.387
MOD_NEK2_1	19	24	PF00069	0.588
MOD_NEK2_1	224	229	PF00069	0.427
MOD_NEK2_1	41	46	PF00069	0.545
MOD_NEK2_1	61	66	PF00069	0.672
MOD_NEK2_1	98	103	PF00069	0.546
MOD_NEK2_2	111	116	PF00069	0.584
MOD_NEK2_2	37	42	PF00069	0.670
MOD_PIKK_1	213	219	PF00454	0.201
MOD_PIKK_1	62	68	PF00454	0.544
MOD_PIKK_1	98	104	PF00454	0.617
MOD_PKA_1	161	167	PF00069	0.453
MOD_PKA_2	137	143	PF00069	0.407
MOD_PKA_2	161	167	PF00069	0.453
MOD_PKA_2	28	34	PF00069	0.554
MOD_PKB_1	15	23	PF00069	0.681
MOD_Plk_1	20	26	PF00069	0.489
MOD_Plk_1	80	86	PF00069	0.589
MOD_Plk_2-3	85	91	PF00069	0.652
MOD_Plk_4	111	117	PF00069	0.557
MOD_Plk_4	146	152	PF00069	0.360
MOD_Plk_4	37	43	PF00069	0.712
MOD_Plk_4	9	15	PF00069	0.474
TRG_DiLeu_BaEn_1	90	95	PF01217	0.542
TRG_ENDOCYTIC_2	148	151	PF00928	0.306
TRG_ENDOCYTIC_2	152	155	PF00928	0.306
TRG_ER_diArg_1	161	163	PF00400	0.453
TRG_NES_CRM1_1	200	214	PF08389	0.427
TRG_Pf-PMV_PEXEL_1	199	204	PF00026	0.363

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I8Z2	Leptomonas seymouri	44%	100%
A0A0S4KLZ2	Bodo saltans	35%	84%
A0A1X0PA72	Trypanosomatidae	36%	100%
A0A3R7K285	Trypanosoma rangeli	36%	89%
A0A3S7X8J3	Leishmania donovani	70%	100%
A4IAJ8	Leishmania infantum	70%	100%
A4IHK8	Xenopus tropicalis	27%	81%
C9ZLP3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	35%	100%
E9B5M2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	69%	100%
Q1RMX9	Bos taurus	22%	100%
Q4Q2B4	Leishmania major	69%	97%
Q4V8B2	Rattus norvegicus	25%	80%
Q54GP1	Dictyostelium discoideum	23%	98%
Q5E9V1	Bos taurus	23%	80%
Q5PPL2	Rattus norvegicus	22%	100%
Q5R9G1	Pongo abelii	23%	80%
Q5RDF9	Pongo abelii	22%	100%
Q5RHX6	Danio rerio	26%	87%
Q6C0B6	Yarrowia lipolytica (strain CLIB 122 / E 150)	22%	100%
Q6DFA1	Xenopus laevis	28%	81%
Q6PH85	Homo sapiens	25%	94%
Q8BZJ7	Mus musculus	23%	94%
Q8CCA0	Mus musculus	25%	84%
Q8IWE4	Homo sapiens	23%	80%
Q8K0V2	Mus musculus	23%	80%
Q92564	Homo sapiens	25%	84%
Q9BTE7	Homo sapiens	22%	100%
Q9CXV9	Mus musculus	22%	100%
Q9U3C8	Caenorhabditis elegans	23%	83%
Q9VUQ8	Drosophila melanogaster	25%	85%
V5BLR0	Trypanosoma cruzi	33%	97%