DNA topoisomerase I

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

DNA topoisomerase I

Gene product:

DNA topoisomerase IB, large subunit

Species:

Leishmania braziliensis

UniProt:

A4HB62_LEIBR

TriTrypDb:

LbrM.20.3020 , LBRM2903_200043800

Length:

637

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	11
GO:0005694	chromosome	5	11
GO:0043226	organelle	2	11
GO:0043227	membrane-bounded organelle	3	11
GO:0043228	non-membrane-bounded organelle	3	11
GO:0043229	intracellular organelle	3	11
GO:0043231	intracellular membrane-bounded organelle	4	11
GO:0043232	intracellular non-membrane-bounded organelle	4	11
GO:0110165	cellular anatomical entity	1	11
GO:0005654	nucleoplasm	2	1
GO:0005730	nucleolus	5	1
GO:0016020	membrane	2	1

Expansion

Sequence features

A4HB62

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HB62

PDB structure(s): 2b9s_A

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	11
GO:0006259	DNA metabolic process	4	11
GO:0006265	DNA topological change	5	11
GO:0006725	cellular aromatic compound metabolic process	3	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0006996	organelle organization	4	11
GO:0008152	metabolic process	1	11
GO:0009987	cellular process	1	11
GO:0016043	cellular component organization	3	11
GO:0034641	cellular nitrogen compound metabolic process	3	11
GO:0043170	macromolecule metabolic process	3	11
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0044260	obsolete cellular macromolecule metabolic process	3	11
GO:0046483	heterocycle metabolic process	3	11
GO:0051276	chromosome organization	5	11
GO:0071103	DNA conformation change	6	11
GO:0071704	organic substance metabolic process	2	11
GO:0071840	cellular component organization or biogenesis	2	11
GO:0090304	nucleic acid metabolic process	4	11
GO:1901360	organic cyclic compound metabolic process	3	11
GO:0006260	DNA replication	5	1
GO:0006355	regulation of DNA-templated transcription	6	1
GO:0007059	chromosome segregation	2	1
GO:0009889	regulation of biosynthetic process	4	1
GO:0010468	regulation of gene expression	5	1
GO:0010556	regulation of macromolecule biosynthetic process	5	1
GO:0019219	regulation of nucleobase-containing compound metabolic process	5	1
GO:0019222	regulation of metabolic process	3	1
GO:0031323	regulation of cellular metabolic process	4	1
GO:0031326	regulation of cellular biosynthetic process	5	1
GO:0050789	regulation of biological process	2	1
GO:0050794	regulation of cellular process	3	1
GO:0051171	regulation of nitrogen compound metabolic process	4	1
GO:0051252	regulation of RNA metabolic process	5	1
GO:0060255	regulation of macromolecule metabolic process	4	1
GO:0065007	biological regulation	1	1
GO:0080090	regulation of primary metabolic process	4	1
GO:1903506	regulation of nucleic acid-templated transcription	7	1
GO:2001141	regulation of RNA biosynthetic process	6	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	11
GO:0003677	DNA binding	4	11
GO:0003824	catalytic activity	1	11
GO:0003916	DNA topoisomerase activity	3	11
GO:0003917	DNA topoisomerase type I (single strand cut, ATP-independent) activity	4	11
GO:0005488	binding	1	11
GO:0016853	isomerase activity	2	11
GO:0097159	organic cyclic compound binding	2	11
GO:0140097	catalytic activity, acting on DNA	3	11
GO:0140640	catalytic activity, acting on a nucleic acid	2	11
GO:1901363	heterocyclic compound binding	2	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_MEL_PAP_1	311	317	PF00089	0.235
CLV_NRD_NRD_1	102	104	PF00675	0.235
CLV_NRD_NRD_1	121	123	PF00675	0.252
CLV_NRD_NRD_1	172	174	PF00675	0.367
CLV_NRD_NRD_1	273	275	PF00675	0.284
CLV_NRD_NRD_1	376	378	PF00675	0.255
CLV_NRD_NRD_1	44	46	PF00675	0.367
CLV_NRD_NRD_1	475	477	PF00675	0.306
CLV_NRD_NRD_1	5	7	PF00675	0.455
CLV_NRD_NRD_1	555	557	PF00675	0.500
CLV_PCSK_KEX2_1	102	104	PF00082	0.235
CLV_PCSK_KEX2_1	11	13	PF00082	0.641
CLV_PCSK_KEX2_1	115	117	PF00082	0.235
CLV_PCSK_KEX2_1	121	123	PF00082	0.235
CLV_PCSK_KEX2_1	172	174	PF00082	0.361
CLV_PCSK_KEX2_1	376	378	PF00082	0.218
CLV_PCSK_KEX2_1	44	46	PF00082	0.235
CLV_PCSK_KEX2_1	531	533	PF00082	0.463
CLV_PCSK_KEX2_1	615	617	PF00082	0.595
CLV_PCSK_PC1ET2_1	11	13	PF00082	0.641
CLV_PCSK_PC1ET2_1	115	117	PF00082	0.279
CLV_PCSK_PC1ET2_1	531	533	PF00082	0.463
CLV_PCSK_PC1ET2_1	615	617	PF00082	0.590
CLV_PCSK_SKI1_1	103	107	PF00082	0.235
CLV_PCSK_SKI1_1	110	114	PF00082	0.235
CLV_PCSK_SKI1_1	151	155	PF00082	0.279
CLV_PCSK_SKI1_1	274	278	PF00082	0.235
CLV_PCSK_SKI1_1	310	314	PF00082	0.235
CLV_PCSK_SKI1_1	420	424	PF00082	0.254
CLV_PCSK_SKI1_1	459	463	PF00082	0.367
CLV_PCSK_SKI1_1	499	503	PF00082	0.506
CLV_PCSK_SKI1_1	532	536	PF00082	0.347
CLV_PCSK_SKI1_1	549	553	PF00082	0.666
CLV_PCSK_SKI1_1	566	570	PF00082	0.600
CLV_PCSK_SKI1_1	618	622	PF00082	0.656
CLV_PCSK_SKI1_1	72	76	PF00082	0.268
DEG_MDM2_SWIB_1	105	113	PF02201	0.235
DEG_Nend_UBRbox_1	1	4	PF02207	0.655
DEG_SCF_FBW7_2	76	81	PF00400	0.235
DEG_SPOP_SBC_1	256	260	PF00917	0.254
DOC_ANK_TNKS_1	179	186	PF00023	0.235
DOC_ANK_TNKS_1	201	208	PF00023	0.154
DOC_CYCLIN_RxL_1	115	129	PF00134	0.235
DOC_MAPK_gen_1	459	468	PF00069	0.311
DOC_MAPK_gen_1	531	537	PF00069	0.337
DOC_PP2B_LxvP_1	55	58	PF13499	0.314
DOC_USP7_MATH_1	256	260	PF00917	0.395
DOC_USP7_MATH_1	426	430	PF00917	0.155
DOC_USP7_MATH_1	568	572	PF00917	0.637
DOC_USP7_MATH_1	588	592	PF00917	0.620
DOC_USP7_UBL2_3	147	151	PF12436	0.238
DOC_USP7_UBL2_3	275	279	PF12436	0.311
DOC_USP7_UBL2_3	455	459	PF12436	0.254
DOC_USP7_UBL2_3	558	562	PF12436	0.620
DOC_USP7_UBL2_3	566	570	PF12436	0.585
DOC_USP7_UBL2_3	573	577	PF12436	0.555
DOC_USP7_UBL2_3	578	582	PF12436	0.586
DOC_USP7_UBL2_3	585	589	PF12436	0.521
DOC_USP7_UBL2_3	7	11	PF12436	0.600
DOC_WW_Pin1_4	376	381	PF00397	0.295
DOC_WW_Pin1_4	584	589	PF00397	0.499
DOC_WW_Pin1_4	74	79	PF00397	0.250
LIG_14-3-3_CanoR_1	284	288	PF00244	0.275
LIG_APCC_ABBA_1	65	70	PF00400	0.268
LIG_BIR_III_2	160	164	PF00653	0.154
LIG_BRCT_BRCA1_1	457	461	PF00533	0.367
LIG_CaM_IQ_9	463	479	PF13499	0.311
LIG_EVH1_2	183	187	PF00568	0.235
LIG_FHA_1	207	213	PF00498	0.254
LIG_FHA_1	609	615	PF00498	0.700
LIG_FHA_2	144	150	PF00498	0.235
LIG_FHA_2	468	474	PF00498	0.348
LIG_FHA_2	75	81	PF00498	0.235
LIG_HCF-1_HBM_1	92	95	PF13415	0.235
LIG_LIR_Gen_1	235	246	PF02991	0.261
LIG_LIR_Gen_1	366	374	PF02991	0.302
LIG_LIR_Gen_1	382	393	PF02991	0.177
LIG_LIR_Gen_1	43	54	PF02991	0.254
LIG_LIR_Gen_1	524	530	PF02991	0.381
LIG_LIR_Gen_1	85	95	PF02991	0.263
LIG_LIR_Nem_3	107	112	PF02991	0.248
LIG_LIR_Nem_3	127	131	PF02991	0.235
LIG_LIR_Nem_3	366	371	PF02991	0.271
LIG_LIR_Nem_3	382	388	PF02991	0.172
LIG_LIR_Nem_3	43	49	PF02991	0.252
LIG_LIR_Nem_3	524	528	PF02991	0.531
LIG_PDZ_Class_2	632	637	PF00595	0.588
LIG_Pex14_1	512	516	PF04695	0.389
LIG_Pex14_2	101	105	PF04695	0.235
LIG_PTB_Apo_2	246	253	PF02174	0.196
LIG_PTB_Phospho_1	246	252	PF10480	0.196
LIG_SH2_CRK	368	372	PF00017	0.279
LIG_SH2_GRB2like	357	360	PF00017	0.235
LIG_SH2_GRB2like	439	442	PF00017	0.235
LIG_SH2_PTP2	252	255	PF00017	0.235
LIG_SH2_SRC	60	63	PF00017	0.314
LIG_SH2_STAP1	474	478	PF00017	0.171
LIG_SH2_STAT5	135	138	PF00017	0.215
LIG_SH2_STAT5	166	169	PF00017	0.311
LIG_SH2_STAT5	252	255	PF00017	0.235
LIG_SH2_STAT5	306	309	PF00017	0.308
LIG_SH2_STAT5	439	442	PF00017	0.235
LIG_SH2_STAT5	474	477	PF00017	0.254
LIG_SH3_3	178	184	PF00018	0.235
LIG_SH3_3	215	221	PF00018	0.248
LIG_SH3_3	51	57	PF00018	0.235
LIG_SUMO_SIM_anti_2	360	366	PF11976	0.314
LIG_SUMO_SIM_anti_2	445	451	PF11976	0.254
LIG_SUMO_SIM_par_1	477	483	PF11976	0.359
LIG_TRAF2_1	146	149	PF00917	0.235
LIG_TRAF2_1	493	496	PF00917	0.472
LIG_TRAF2_1	591	594	PF00917	0.580
LIG_TRAF2_1	77	80	PF00917	0.236
LIG_TRAF2_2	390	395	PF00917	0.367
LIG_UBA3_1	195	200	PF00899	0.254
LIG_UBA3_1	465	471	PF00899	0.348
LIG_UBA3_1	478	484	PF00899	0.261
LIG_UBA3_1	86	91	PF00899	0.367
LIG_WRC_WIRS_1	397	402	PF05994	0.225
LIG_WW_1	57	60	PF00397	0.235
MOD_CDK_SPK_2	584	589	PF00069	0.499
MOD_CDK_SPxK_1	584	590	PF00069	0.501
MOD_CK1_1	379	385	PF00069	0.268
MOD_CK1_1	429	435	PF00069	0.154
MOD_CK1_1	472	478	PF00069	0.242
MOD_CK1_1	515	521	PF00069	0.516
MOD_CK1_1	545	551	PF00069	0.546
MOD_CK1_1	572	578	PF00069	0.565
MOD_CK1_1	580	586	PF00069	0.599
MOD_CK2_1	105	111	PF00069	0.236
MOD_CK2_1	143	149	PF00069	0.240
MOD_CK2_1	283	289	PF00069	0.362
MOD_CK2_1	467	473	PF00069	0.439
MOD_CK2_1	490	496	PF00069	0.539
MOD_CK2_1	588	594	PF00069	0.648
MOD_CK2_1	74	80	PF00069	0.235
MOD_Cter_Amidation	170	173	PF01082	0.235
MOD_Cter_Amidation	539	542	PF01082	0.501
MOD_Cter_Amidation	560	563	PF01082	0.662
MOD_GlcNHglycan	404	407	PF01048	0.239
MOD_GlcNHglycan	538	541	PF01048	0.567
MOD_GlcNHglycan	545	548	PF01048	0.597
MOD_GlcNHglycan	552	555	PF01048	0.512
MOD_GSK3_1	372	379	PF00069	0.367
MOD_GSK3_1	408	415	PF00069	0.235
MOD_GSK3_1	536	543	PF00069	0.459
MOD_GSK3_1	568	575	PF00069	0.535
MOD_GSK3_1	577	584	PF00069	0.505
MOD_LATS_1	453	459	PF00433	0.254
MOD_N-GLC_1	143	148	PF02516	0.284
MOD_N-GLC_1	412	417	PF02516	0.235
MOD_NEK2_1	105	110	PF00069	0.236
MOD_NEK2_1	255	260	PF00069	0.154
MOD_NEK2_1	466	471	PF00069	0.385
MOD_NEK2_2	257	262	PF00069	0.254
MOD_NEK2_2	328	333	PF00069	0.235
MOD_NEK2_2	351	356	PF00069	0.235
MOD_PIKK_1	388	394	PF00454	0.367
MOD_PK_1	490	496	PF00069	0.539
MOD_PKA_1	490	496	PF00069	0.464
MOD_PKA_1	541	547	PF00069	0.594
MOD_PKA_1	562	568	PF00069	0.618
MOD_PKA_1	569	575	PF00069	0.574
MOD_PKA_1	577	583	PF00069	0.561
MOD_PKA_2	242	248	PF00069	0.225
MOD_PKA_2	283	289	PF00069	0.254
MOD_PKA_2	372	378	PF00069	0.254
MOD_Plk_1	232	238	PF00069	0.242
MOD_Plk_1	412	418	PF00069	0.235
MOD_Plk_2-3	149	155	PF00069	0.154
MOD_Plk_2-3	622	628	PF00069	0.474
MOD_Plk_4	234	240	PF00069	0.235
MOD_Plk_4	242	248	PF00069	0.235
MOD_Plk_4	283	289	PF00069	0.364
MOD_Plk_4	396	402	PF00069	0.240
MOD_Plk_4	599	605	PF00069	0.469
MOD_Plk_4	82	88	PF00069	0.235
MOD_ProDKin_1	376	382	PF00069	0.295
MOD_ProDKin_1	584	590	PF00069	0.501
MOD_ProDKin_1	74	80	PF00069	0.250
MOD_SUMO_for_1	1	4	PF00179	0.577
MOD_SUMO_for_1	10	13	PF00179	0.615
MOD_SUMO_for_1	268	271	PF00179	0.235
MOD_SUMO_for_1	40	43	PF00179	0.500
MOD_SUMO_rev_2	138	143	PF00179	0.217
MOD_SUMO_rev_2	148	153	PF00179	0.260
MOD_SUMO_rev_2	286	293	PF00179	0.342
MOD_SUMO_rev_2	4	9	PF00179	0.470
MOD_SUMO_rev_2	424	432	PF00179	0.314
MOD_SUMO_rev_2	70	77	PF00179	0.254
TRG_DiLeu_BaEn_1	335	340	PF01217	0.367
TRG_DiLeu_BaEn_1	366	371	PF01217	0.367
TRG_ENDOCYTIC_2	252	255	PF00928	0.237
TRG_ENDOCYTIC_2	368	371	PF00928	0.235
TRG_ENDOCYTIC_2	95	98	PF00928	0.367
TRG_ER_diArg_1	101	103	PF00400	0.235
TRG_ER_diArg_1	120	122	PF00400	0.254
TRG_ER_diArg_1	187	190	PF00400	0.235
TRG_ER_diArg_1	44	46	PF00400	0.367
TRG_NLS_Bipartite_1	476	494	PF00514	0.436
TRG_NLS_Bipartite_1	531	553	PF00514	0.574
TRG_NLS_Bipartite_1	577	593	PF00514	0.544
TRG_NLS_MonoCore_2	555	560	PF00514	0.642
TRG_NLS_MonoExtC_3	489	495	PF00514	0.512
TRG_NLS_MonoExtC_3	561	566	PF00514	0.535
TRG_NLS_MonoExtC_3	588	594	PF00514	0.521
TRG_NLS_MonoExtN_4	556	561	PF00514	0.577
TRG_NLS_MonoExtN_4	562	567	PF00514	0.577
TRG_NLS_MonoExtN_4	586	593	PF00514	0.555
TRG_Pf-PMV_PEXEL_1	110	114	PF00026	0.208
TRG_Pf-PMV_PEXEL_1	274	278	PF00026	0.314

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0L0P4F8	Candida auris	50%	85%
A0A0N0P5Q3	Leptomonas seymouri	79%	98%
A0A0S4IPT9	Bodo saltans	51%	98%
A0A1X0PA36	Trypanosomatidae	73%	96%
A0A3Q8IHS4	Leishmania donovani	89%	100%
A0A3R7MDP0	Trypanosoma rangeli	65%	96%
A9Q1D5	Pan troglodytes	44%	100%
C9ZLY6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	59%	92%
E9B5D3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	85%	100%
O17966	Caenorhabditis elegans	48%	79%
P04786	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	43%	83%
P07799	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	52%	78%
P11387	Homo sapiens	51%	83%
P30181	Arabidopsis thaliana	52%	70%
P41512	Xenopus laevis	48%	77%
P93119	Daucus carota	48%	81%
Q00313	Candida albicans	49%	82%
Q04750	Mus musculus	51%	83%
Q07050	Cricetulus griseus	50%	83%
Q4Q2K0	Leishmania major	89%	100%
Q54RC3	Dictyostelium discoideum	49%	71%
Q6IM78	Rattus norvegicus	44%	100%
Q7YR26	Chlorocebus aethiops	51%	83%
Q8R4U6	Mus musculus	44%	100%
Q969P6	Homo sapiens	44%	100%
Q9FJ79	Arabidopsis thaliana	53%	69%
Q9GPZ9	Leishmania infantum	89%	100%
Q9WUL0	Rattus norvegicus	51%	83%
V5BQI4	Trypanosoma cruzi	63%	96%