Putative cleavage and polyadenylation specificity factor

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

cleavage and polyadenylation specificity factor, putative

Species:

Leishmania braziliensis

UniProt:

A4HB61_LEIBR

TriTrypDb:

LbrM.20.3010 , LBRM2903_200043600

Length:

756

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	11
GO:0043226	organelle	2	11
GO:0043227	membrane-bounded organelle	3	11
GO:0043229	intracellular organelle	3	11
GO:0043231	intracellular membrane-bounded organelle	4	11
GO:0110165	cellular anatomical entity	1	12
GO:0005654	nucleoplasm	2	1
GO:0005847	mRNA cleavage and polyadenylation specificity factor complex	4	1
GO:0005849	mRNA cleavage factor complex	3	1
GO:0032991	protein-containing complex	1	1
GO:0140513	nuclear protein-containing complex	2	1

Expansion

Sequence features

A4HB61

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HB61

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006396	RNA processing	6	12
GO:0006397	mRNA processing	7	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0016071	mRNA metabolic process	6	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0043170	macromolecule metabolic process	3	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:0006378	mRNA polyadenylation	7	1
GO:0006398	mRNA 3'-end processing by stem-loop binding and cleavage	8	1
GO:0008334	histone mRNA metabolic process	7	1
GO:0031123	RNA 3'-end processing	7	1
GO:0031124	mRNA 3'-end processing	8	1
GO:0043631	RNA polyadenylation	6	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	1
GO:0003723	RNA binding	4	1
GO:0003824	catalytic activity	1	2
GO:0004518	nuclease activity	4	1
GO:0004519	endonuclease activity	5	1
GO:0004521	RNA endonuclease activity	5	1
GO:0004527	exonuclease activity	5	1
GO:0004540	RNA nuclease activity	4	1
GO:0005488	binding	1	1
GO:0008409	5'-3' exonuclease activity	6	1
GO:0016787	hydrolase activity	2	2
GO:0016788	hydrolase activity, acting on ester bonds	3	1
GO:0097159	organic cyclic compound binding	2	1
GO:0140098	catalytic activity, acting on RNA	3	1
GO:0140640	catalytic activity, acting on a nucleic acid	2	1
GO:1901363	heterocyclic compound binding	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	62	66	PF00656	0.234
CLV_C14_Caspase3-7	686	690	PF00656	0.379
CLV_NRD_NRD_1	243	245	PF00675	0.454
CLV_NRD_NRD_1	361	363	PF00675	0.223
CLV_PCSK_KEX2_1	243	245	PF00082	0.454
CLV_PCSK_KEX2_1	361	363	PF00082	0.210
CLV_PCSK_KEX2_1	727	729	PF00082	0.283
CLV_PCSK_PC1ET2_1	727	729	PF00082	0.237
CLV_PCSK_SKI1_1	198	202	PF00082	0.249
CLV_PCSK_SKI1_1	289	293	PF00082	0.199
CLV_PCSK_SKI1_1	378	382	PF00082	0.280
CLV_PCSK_SKI1_1	474	478	PF00082	0.384
CLV_PCSK_SKI1_1	593	597	PF00082	0.340
CLV_PCSK_SKI1_1	723	727	PF00082	0.249
DEG_APCC_DBOX_1	535	543	PF00400	0.379
DOC_MAPK_DCC_7	389	399	PF00069	0.381
DOC_MAPK_DCC_7	728	738	PF00069	0.158
DOC_MAPK_gen_1	243	252	PF00069	0.367
DOC_MAPK_gen_1	425	432	PF00069	0.474
DOC_MAPK_gen_1	534	541	PF00069	0.268
DOC_MAPK_gen_1	727	733	PF00069	0.237
DOC_MAPK_MEF2A_6	257	265	PF00069	0.234
DOC_MAPK_MEF2A_6	523	532	PF00069	0.379
DOC_MAPK_MEF2A_6	74	82	PF00069	0.249
DOC_PP2B_LxvP_1	530	533	PF13499	0.249
DOC_PP2B_PxIxI_1	209	215	PF00149	0.280
DOC_PP4_FxxP_1	734	737	PF00568	0.386
DOC_USP7_MATH_1	129	133	PF00917	0.379
DOC_USP7_MATH_1	30	34	PF00917	0.239
DOC_USP7_MATH_1	483	487	PF00917	0.610
DOC_USP7_MATH_1	560	564	PF00917	0.403
DOC_USP7_MATH_1	59	63	PF00917	0.249
DOC_USP7_MATH_1	649	653	PF00917	0.353
DOC_USP7_UBL2_3	114	118	PF12436	0.234
DOC_USP7_UBL2_3	385	389	PF12436	0.363
DOC_USP7_UBL2_3	477	481	PF12436	0.508
DOC_USP7_UBL2_3	723	727	PF12436	0.253
DOC_WW_Pin1_4	206	211	PF00397	0.308
DOC_WW_Pin1_4	338	343	PF00397	0.423
DOC_WW_Pin1_4	534	539	PF00397	0.330
LIG_14-3-3_CanoR_1	106	116	PF00244	0.234
LIG_14-3-3_CanoR_1	398	405	PF00244	0.445
LIG_14-3-3_CanoR_1	453	458	PF00244	0.460
LIG_14-3-3_CanoR_1	728	734	PF00244	0.243
LIG_Actin_WH2_2	112	128	PF00022	0.234
LIG_Actin_WH2_2	475	490	PF00022	0.428
LIG_APCC_ABBA_1	513	518	PF00400	0.268
LIG_APCC_ABBA_1	699	704	PF00400	0.299
LIG_BIR_II_1	1	5	PF00653	0.653
LIG_BIR_III_1	1	5	PF00653	0.653
LIG_BIR_III_3	1	5	PF00653	0.653
LIG_BRCT_BRCA1_1	551	555	PF00533	0.234
LIG_BRCT_BRCA1_1	65	69	PF00533	0.249
LIG_CaM_IQ_9	539	555	PF13499	0.355
LIG_Clathr_ClatBox_1	262	266	PF01394	0.399
LIG_EH_1	313	317	PF12763	0.399
LIG_eIF4E_1	208	214	PF01652	0.302
LIG_FHA_1	216	222	PF00498	0.320
LIG_FHA_1	233	239	PF00498	0.315
LIG_FHA_1	427	433	PF00498	0.410
LIG_FHA_1	473	479	PF00498	0.387
LIG_FHA_1	579	585	PF00498	0.222
LIG_FHA_1	594	600	PF00498	0.325
LIG_FHA_1	606	612	PF00498	0.237
LIG_FHA_2	562	568	PF00498	0.263
LIG_FHA_2	684	690	PF00498	0.233
LIG_GBD_Chelix_1	695	703	PF00786	0.299
LIG_KLC1_Yacidic_2	700	704	PF13176	0.299
LIG_LIR_Apic_2	711	717	PF02991	0.330
LIG_LIR_Apic_2	732	737	PF02991	0.249
LIG_LIR_Gen_1	123	131	PF02991	0.249
LIG_LIR_Gen_1	189	200	PF02991	0.249
LIG_LIR_Gen_1	456	466	PF02991	0.384
LIG_LIR_Gen_1	552	560	PF02991	0.268
LIG_LIR_Gen_1	615	625	PF02991	0.328
LIG_LIR_Gen_1	668	678	PF02991	0.262
LIG_LIR_Nem_3	123	127	PF02991	0.249
LIG_LIR_Nem_3	162	166	PF02991	0.379
LIG_LIR_Nem_3	189	195	PF02991	0.249
LIG_LIR_Nem_3	552	558	PF02991	0.234
LIG_LIR_Nem_3	615	621	PF02991	0.328
LIG_LIR_Nem_3	668	674	PF02991	0.249
LIG_Rb_LxCxE_1	71	89	PF01857	0.234
LIG_SH2_CRK	117	121	PF00017	0.249
LIG_SH2_CRK	505	509	PF00017	0.249
LIG_SH2_CRK	671	675	PF00017	0.208
LIG_SH2_NCK_1	714	718	PF00017	0.379
LIG_SH2_SRC	320	323	PF00017	0.420
LIG_SH2_SRC	702	705	PF00017	0.156
LIG_SH2_STAP1	282	286	PF00017	0.399
LIG_SH2_STAP1	614	618	PF00017	0.352
LIG_SH2_STAT3	548	551	PF00017	0.234
LIG_SH2_STAT5	188	191	PF00017	0.234
LIG_SH2_STAT5	320	323	PF00017	0.410
LIG_SH2_STAT5	370	373	PF00017	0.420
LIG_SH2_STAT5	458	461	PF00017	0.415
LIG_SH2_STAT5	548	551	PF00017	0.234
LIG_SH2_STAT5	702	705	PF00017	0.392
LIG_SH2_STAT5	730	733	PF00017	0.297
LIG_SH2_STAT5	96	99	PF00017	0.234
LIG_SH3_3	1	7	PF00018	0.602
LIG_SH3_3	204	210	PF00018	0.339
LIG_SH3_3	384	390	PF00018	0.422
LIG_SH3_3	642	648	PF00018	0.379
LIG_SH3_4	389	396	PF00018	0.397
LIG_SUMO_SIM_anti_2	24	30	PF11976	0.327
LIG_SUMO_SIM_anti_2	260	267	PF11976	0.399
LIG_SUMO_SIM_anti_2	494	500	PF11976	0.293
LIG_SUMO_SIM_anti_2	537	543	PF11976	0.268
LIG_SUMO_SIM_anti_2	76	82	PF11976	0.234
LIG_SUMO_SIM_par_1	260	267	PF11976	0.399
LIG_SUMO_SIM_par_1	607	612	PF11976	0.287
LIG_SUMO_SIM_par_1	79	84	PF11976	0.234
LIG_TRAF2_1	226	229	PF00917	0.376
LIG_TRAF2_1	564	567	PF00917	0.392
LIG_WRC_WIRS_1	294	299	PF05994	0.405
MOD_CK1_1	347	353	PF00069	0.419
MOD_CK1_1	408	414	PF00069	0.400
MOD_CK1_1	456	462	PF00069	0.523
MOD_CK1_1	665	671	PF00069	0.245
MOD_CK1_1	673	679	PF00069	0.227
MOD_CK2_1	223	229	PF00069	0.361
MOD_CK2_1	30	36	PF00069	0.312
MOD_CK2_1	397	403	PF00069	0.503
MOD_CK2_1	534	540	PF00069	0.271
MOD_CK2_1	561	567	PF00069	0.348
MOD_Cter_Amidation	707	710	PF01082	0.379
MOD_GlcNHglycan	110	113	PF01048	0.234
MOD_GlcNHglycan	298	301	PF01048	0.210
MOD_GlcNHglycan	32	35	PF01048	0.280
MOD_GlcNHglycan	346	349	PF01048	0.210
MOD_GlcNHglycan	407	410	PF01048	0.199
MOD_GlcNHglycan	493	496	PF01048	0.270
MOD_GlcNHglycan	499	502	PF01048	0.286
MOD_GlcNHglycan	61	64	PF01048	0.249
MOD_GlcNHglycan	650	654	PF01048	0.283
MOD_GlcNHglycan	679	682	PF01048	0.379
MOD_GSK3_1	129	136	PF00069	0.328
MOD_GSK3_1	186	193	PF00069	0.234
MOD_GSK3_1	320	327	PF00069	0.410
MOD_GSK3_1	453	460	PF00069	0.443
MOD_GSK3_1	589	596	PF00069	0.279
MOD_GSK3_1	59	66	PF00069	0.241
MOD_GSK3_1	600	607	PF00069	0.341
MOD_GSK3_1	609	616	PF00069	0.258
MOD_GSK3_1	665	672	PF00069	0.247
MOD_GSK3_1	673	680	PF00069	0.269
MOD_N-GLC_1	453	458	PF02516	0.551
MOD_N-GLC_1	483	488	PF02516	0.596
MOD_N-GLC_1	676	681	PF02516	0.396
MOD_N-GLC_1	98	103	PF02516	0.379
MOD_NEK2_1	108	113	PF00069	0.234
MOD_NEK2_1	233	238	PF00069	0.332
MOD_NEK2_1	293	298	PF00069	0.423
MOD_NEK2_1	324	329	PF00069	0.410
MOD_NEK2_1	344	349	PF00069	0.466
MOD_NEK2_1	397	402	PF00069	0.508
MOD_NEK2_1	497	502	PF00069	0.234
MOD_NEK2_1	549	554	PF00069	0.239
MOD_NEK2_1	584	589	PF00069	0.156
MOD_NEK2_1	609	614	PF00069	0.268
MOD_NEK2_1	670	675	PF00069	0.208
MOD_NEK2_1	69	74	PF00069	0.249
MOD_NEK2_1	703	708	PF00069	0.379
MOD_NEK2_2	483	488	PF00069	0.441
MOD_PIKK_1	600	606	PF00454	0.291
MOD_PIKK_1	665	671	PF00454	0.265
MOD_PK_1	453	459	PF00069	0.392
MOD_PKA_2	397	403	PF00069	0.508
MOD_PKA_2	413	419	PF00069	0.399
MOD_PKA_2	560	566	PF00069	0.327
MOD_PKB_1	451	459	PF00069	0.404
MOD_Plk_1	154	160	PF00069	0.249
MOD_Plk_1	190	196	PF00069	0.249
MOD_Plk_1	453	459	PF00069	0.392
MOD_Plk_1	483	489	PF00069	0.469
MOD_Plk_1	517	523	PF00069	0.307
MOD_Plk_1	584	590	PF00069	0.290
MOD_Plk_1	593	599	PF00069	0.239
MOD_Plk_1	623	629	PF00069	0.234
MOD_Plk_1	9	15	PF00069	0.429
MOD_Plk_1	98	104	PF00069	0.249
MOD_Plk_2-3	326	332	PF00069	0.410
MOD_Plk_4	293	299	PF00069	0.404
MOD_Plk_4	320	326	PF00069	0.390
MOD_Plk_4	426	432	PF00069	0.503
MOD_Plk_4	453	459	PF00069	0.392
MOD_Plk_4	503	509	PF00069	0.274
MOD_Plk_4	584	590	PF00069	0.299
MOD_Plk_4	662	668	PF00069	0.247
MOD_Plk_4	729	735	PF00069	0.271
MOD_ProDKin_1	206	212	PF00069	0.303
MOD_ProDKin_1	338	344	PF00069	0.423
MOD_ProDKin_1	534	540	PF00069	0.330
MOD_SUMO_rev_2	323	330	PF00179	0.503
MOD_SUMO_rev_2	386	395	PF00179	0.420
TRG_ENDOCYTIC_2	117	120	PF00928	0.249
TRG_ENDOCYTIC_2	294	297	PF00928	0.423
TRG_ENDOCYTIC_2	458	461	PF00928	0.466
TRG_ENDOCYTIC_2	505	508	PF00928	0.249
TRG_ENDOCYTIC_2	618	621	PF00928	0.328
TRG_ENDOCYTIC_2	671	674	PF00928	0.234
TRG_ENDOCYTIC_2	730	733	PF00928	0.234
TRG_ER_diArg_1	242	244	PF00400	0.442
TRG_ER_diArg_1	533	536	PF00400	0.358
TRG_NES_CRM1_1	76	89	PF08389	0.236

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I3U6	Leptomonas seymouri	91%	100%
A0A0S4ILM6	Bodo saltans	63%	100%
A0A1X0P9Y6	Trypanosomatidae	76%	97%
A0A3Q8ILE8	Leishmania donovani	96%	100%
A0A422NEN5	Trypanosoma rangeli	76%	99%
A4IAA9	Leishmania infantum	96%	100%
C9ZLY7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	75%	98%
E9B5D2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	95%	100%
O13794	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	46%	100%
P0CM88	Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565)	42%	98%
P0CM89	Cryptococcus neoformans var. neoformans serotype D (strain B-3501A)	42%	98%
P79101	Bos taurus	44%	100%
Q4IPN9	Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1)	44%	91%
Q4PEJ3	Ustilago maydis (strain 521 / FGSC 9021)	41%	86%
Q4Q2K1	Leishmania major	96%	100%
Q4WRC2	Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100)	40%	87%
Q5BEP0	Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)	40%	86%
Q6C2Z7	Yarrowia lipolytica (strain CLIB 122 / E 150)	45%	91%
Q6CUI5	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)	44%	99%
Q6FUA5	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	45%	98%
Q86A79	Dictyostelium discoideum	44%	98%
Q8GUU3	Arabidopsis thaliana	32%	100%
Q8WZS6	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)	38%	89%
Q9C952	Arabidopsis thaliana	41%	100%
Q9QXK7	Mus musculus	43%	100%
Q9UKF6	Homo sapiens	47%	100%
Q9VE51	Drosophila melanogaster	52%	100%
V5DM01	Trypanosoma cruzi	76%	99%