Asparagine--tRNA ligase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

asparaginyl-tRNA synthetase, putative

Species:

Leishmania braziliensis

UniProt:

A4HAU6_LEIBR

TriTrypDb:

LbrM.20.1840 , LBRM2903_200029000

Length:

895

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 3
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 20

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 7

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	12
GO:0110165	cellular anatomical entity	1	12

Expansion

Sequence features

A4HAU6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HAU6

Function

Biological processes
Term	Name	Level	Count
GO:0006082	organic acid metabolic process	3	12
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006399	tRNA metabolic process	7	12
GO:0006418	tRNA aminoacylation for protein translation	6	12
GO:0006421	asparaginyl-tRNA aminoacylation	7	10
GO:0006520	amino acid metabolic process	3	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0019752	carboxylic acid metabolic process	5	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0034660	ncRNA metabolic process	6	12
GO:0043038	amino acid activation	4	12
GO:0043039	tRNA aminoacylation	5	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043436	oxoacid metabolic process	4	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044281	small molecule metabolic process	2	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:1901564	organonitrogen compound metabolic process	3	12

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	12
GO:0003676	nucleic acid binding	3	12
GO:0003824	catalytic activity	1	12
GO:0004812	aminoacyl-tRNA ligase activity	4	12
GO:0004816	asparagine-tRNA ligase activity	5	10
GO:0005488	binding	1	12
GO:0005524	ATP binding	5	12
GO:0016874	ligase activity	2	12
GO:0016875	ligase activity, forming carbon-oxygen bonds	3	12
GO:0017076	purine nucleotide binding	4	12
GO:0030554	adenyl nucleotide binding	5	12
GO:0032553	ribonucleotide binding	3	12
GO:0032555	purine ribonucleotide binding	4	12
GO:0032559	adenyl ribonucleotide binding	5	12
GO:0035639	purine ribonucleoside triphosphate binding	4	12
GO:0036094	small molecule binding	2	12
GO:0043167	ion binding	2	12
GO:0043168	anion binding	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:0097367	carbohydrate derivative binding	2	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140101	catalytic activity, acting on a tRNA	4	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:1901265	nucleoside phosphate binding	3	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	719	723	PF00656	0.531
CLV_C14_Caspase3-7	831	835	PF00656	0.589
CLV_C14_Caspase3-7	839	843	PF00656	0.496
CLV_NRD_NRD_1	176	178	PF00675	0.743
CLV_NRD_NRD_1	217	219	PF00675	0.686
CLV_NRD_NRD_1	294	296	PF00675	0.340
CLV_NRD_NRD_1	380	382	PF00675	0.373
CLV_NRD_NRD_1	430	432	PF00675	0.674
CLV_NRD_NRD_1	595	597	PF00675	0.340
CLV_NRD_NRD_1	69	71	PF00675	0.650
CLV_NRD_NRD_1	735	737	PF00675	0.298
CLV_NRD_NRD_1	853	855	PF00675	0.298
CLV_NRD_NRD_1	97	99	PF00675	0.753
CLV_PCSK_FUR_1	174	178	PF00082	0.757
CLV_PCSK_KEX2_1	176	178	PF00082	0.743
CLV_PCSK_KEX2_1	380	382	PF00082	0.312
CLV_PCSK_KEX2_1	595	597	PF00082	0.340
CLV_PCSK_KEX2_1	661	663	PF00082	0.287
CLV_PCSK_KEX2_1	69	71	PF00082	0.758
CLV_PCSK_KEX2_1	731	733	PF00082	0.298
CLV_PCSK_KEX2_1	735	737	PF00082	0.276
CLV_PCSK_KEX2_1	837	839	PF00082	0.344
CLV_PCSK_KEX2_1	853	855	PF00082	0.277
CLV_PCSK_KEX2_1	97	99	PF00082	0.509
CLV_PCSK_PC1ET2_1	661	663	PF00082	0.348
CLV_PCSK_PC1ET2_1	731	733	PF00082	0.372
CLV_PCSK_PC1ET2_1	837	839	PF00082	0.373
CLV_PCSK_SKI1_1	138	142	PF00082	0.574
CLV_PCSK_SKI1_1	176	180	PF00082	0.755
CLV_PCSK_SKI1_1	246	250	PF00082	0.312
CLV_PCSK_SKI1_1	279	283	PF00082	0.298
CLV_PCSK_SKI1_1	394	398	PF00082	0.445
CLV_PCSK_SKI1_1	92	96	PF00082	0.757
CLV_Separin_Metazoa	583	587	PF03568	0.528
DEG_SPOP_SBC_1	140	144	PF00917	0.725
DOC_CYCLIN_yCln2_LP_2	527	533	PF00134	0.498
DOC_MAPK_gen_1	254	264	PF00069	0.497
DOC_MAPK_gen_1	367	376	PF00069	0.573
DOC_MAPK_gen_1	475	484	PF00069	0.495
DOC_MAPK_gen_1	735	745	PF00069	0.487
DOC_MAPK_gen_1	97	103	PF00069	0.507
DOC_MAPK_MEF2A_6	45	54	PF00069	0.489
DOC_MAPK_RevD_3	162	177	PF00069	0.715
DOC_MAPK_RevD_3	280	296	PF00069	0.410
DOC_PP1_RVXF_1	867	874	PF00149	0.487
DOC_PP2B_LxvP_1	101	104	PF13499	0.593
DOC_PP2B_LxvP_1	166	169	PF13499	0.700
DOC_PP2B_LxvP_1	187	190	PF13499	0.499
DOC_PP2B_LxvP_1	527	530	PF13499	0.498
DOC_USP7_MATH_1	140	144	PF00917	0.713
DOC_USP7_MATH_1	297	301	PF00917	0.446
DOC_USP7_MATH_1	554	558	PF00917	0.537
DOC_USP7_MATH_1	655	659	PF00917	0.496
DOC_USP7_MATH_2	181	187	PF00917	0.503
DOC_USP7_UBL2_3	657	661	PF12436	0.464
DOC_USP7_UBL2_3	727	731	PF12436	0.496
DOC_WW_Pin1_4	106	111	PF00397	0.709
DOC_WW_Pin1_4	156	161	PF00397	0.617
DOC_WW_Pin1_4	271	276	PF00397	0.492
DOC_WW_Pin1_4	598	603	PF00397	0.498
DOC_WW_Pin1_4	726	731	PF00397	0.565
DOC_WW_Pin1_4	82	87	PF00397	0.756
LIG_14-3-3_CanoR_1	114	119	PF00244	0.522
LIG_14-3-3_CanoR_1	138	148	PF00244	0.702
LIG_14-3-3_CanoR_1	218	224	PF00244	0.650
LIG_14-3-3_CanoR_1	444	451	PF00244	0.348
LIG_14-3-3_CanoR_1	45	49	PF00244	0.700
LIG_14-3-3_CanoR_1	481	485	PF00244	0.498
LIG_14-3-3_CanoR_1	662	670	PF00244	0.489
LIG_14-3-3_CanoR_1	97	102	PF00244	0.743
LIG_Actin_WH2_2	100	116	PF00022	0.496
LIG_Actin_WH2_2	372	388	PF00022	0.548
LIG_BRCT_BRCA1_1	613	617	PF00533	0.487
LIG_eIF4E_1	827	833	PF01652	0.573
LIG_FHA_1	127	133	PF00498	0.673
LIG_FHA_1	140	146	PF00498	0.497
LIG_FHA_1	22	28	PF00498	0.475
LIG_FHA_1	308	314	PF00498	0.453
LIG_FHA_1	413	419	PF00498	0.497
LIG_FHA_1	638	644	PF00498	0.508
LIG_FHA_1	709	715	PF00498	0.544
LIG_FHA_1	804	810	PF00498	0.487
LIG_FHA_2	218	224	PF00498	0.626
LIG_FHA_2	321	327	PF00498	0.520
LIG_FHA_2	33	39	PF00498	0.693
LIG_FHA_2	349	355	PF00498	0.503
LIG_FHA_2	606	612	PF00498	0.494
LIG_FHA_2	613	619	PF00498	0.476
LIG_FHA_2	666	672	PF00498	0.498
LIG_FHA_2	694	700	PF00498	0.586
LIG_FHA_2	802	808	PF00498	0.499
LIG_LIR_Gen_1	483	489	PF02991	0.491
LIG_LIR_Gen_1	537	545	PF02991	0.545
LIG_LIR_Gen_1	614	622	PF02991	0.479
LIG_LIR_Gen_1	665	674	PF02991	0.589
LIG_LIR_Gen_1	678	686	PF02991	0.487
LIG_LIR_Gen_1	780	789	PF02991	0.573
LIG_LIR_Nem_3	193	198	PF02991	0.589
LIG_LIR_Nem_3	441	446	PF02991	0.459
LIG_LIR_Nem_3	483	487	PF02991	0.491
LIG_LIR_Nem_3	537	541	PF02991	0.534
LIG_LIR_Nem_3	594	600	PF02991	0.579
LIG_LIR_Nem_3	614	620	PF02991	0.357
LIG_LIR_Nem_3	665	670	PF02991	0.591
LIG_LIR_Nem_3	678	682	PF02991	0.423
LIG_LIR_Nem_3	844	849	PF02991	0.506
LIG_Pex14_1	593	597	PF04695	0.573
LIG_Pex14_2	588	592	PF04695	0.498
LIG_Pex14_2	783	787	PF04695	0.528
LIG_Rb_pABgroove_1	556	564	PF01858	0.460
LIG_SH2_CRK	195	199	PF00017	0.634
LIG_SH2_CRK	538	542	PF00017	0.410
LIG_SH2_CRK	647	651	PF00017	0.440
LIG_SH2_NCK_1	538	542	PF00017	0.410
LIG_SH2_SRC	538	541	PF00017	0.311
LIG_SH2_SRC	739	742	PF00017	0.528
LIG_SH2_STAP1	739	743	PF00017	0.512
LIG_SH2_STAP1	829	833	PF00017	0.501
LIG_SH2_STAT3	627	630	PF00017	0.512
LIG_SH2_STAT3	849	852	PF00017	0.487
LIG_SH2_STAT5	468	471	PF00017	0.548
LIG_SH2_STAT5	591	594	PF00017	0.414
LIG_SH2_STAT5	627	630	PF00017	0.488
LIG_SH2_STAT5	635	638	PF00017	0.470
LIG_SH2_STAT5	647	650	PF00017	0.357
LIG_SH2_STAT5	849	852	PF00017	0.487
LIG_SH3_1	727	733	PF00018	0.492
LIG_SH3_2	730	735	PF14604	0.487
LIG_SH3_2	87	92	PF14604	0.749
LIG_SH3_3	413	419	PF00018	0.576
LIG_SH3_3	597	603	PF00018	0.487
LIG_SH3_3	727	733	PF00018	0.500
LIG_SH3_3	808	814	PF00018	0.476
LIG_SH3_3	84	90	PF00018	0.749
LIG_SH3_CIN85_PxpxPR_1	133	138	PF14604	0.488
LIG_SUMO_SIM_anti_2	141	149	PF11976	0.485
LIG_SUMO_SIM_anti_2	51	57	PF11976	0.485
LIG_SUMO_SIM_anti_2	806	813	PF11976	0.487
LIG_TRAF2_1	422	425	PF00917	0.795
LIG_TYR_ITSM	191	198	PF00017	0.480
LIG_UBA3_1	404	413	PF00899	0.408
LIG_UBA3_1	874	881	PF00899	0.573
LIG_WRC_WIRS_1	298	303	PF05994	0.500
LIG_WRC_WIRS_1	676	681	PF05994	0.573
LIG_WRC_WIRS_1	791	796	PF05994	0.487
LIG_WW_3	215	219	PF00397	0.524
MOD_CDK_SPK_2	271	276	PF00069	0.487
MOD_CDK_SPK_2	726	731	PF00069	0.535
MOD_CDK_SPxK_1	726	732	PF00069	0.573
MOD_CK1_1	106	112	PF00069	0.484
MOD_CK1_1	128	134	PF00069	0.684
MOD_CK1_1	152	158	PF00069	0.646
MOD_CK1_1	480	486	PF00069	0.498
MOD_CK1_1	544	550	PF00069	0.476
MOD_CK1_1	665	671	PF00069	0.487
MOD_CK1_1	81	87	PF00069	0.735
MOD_CK2_1	217	223	PF00069	0.632
MOD_CK2_1	32	38	PF00069	0.692
MOD_CK2_1	320	326	PF00069	0.510
MOD_CK2_1	348	354	PF00069	0.465
MOD_CK2_1	457	463	PF00069	0.485
MOD_CK2_1	605	611	PF00069	0.512
MOD_CK2_1	665	671	PF00069	0.487
MOD_CK2_1	693	699	PF00069	0.535
MOD_CK2_1	801	807	PF00069	0.491
MOD_CMANNOS	25	28	PF00535	0.472
MOD_GlcNHglycan	133	136	PF01048	0.726
MOD_GlcNHglycan	359	364	PF01048	0.210
MOD_GlcNHglycan	463	466	PF01048	0.323
MOD_GlcNHglycan	489	493	PF01048	0.225
MOD_GlcNHglycan	80	83	PF01048	0.642
MOD_GSK3_1	149	156	PF00069	0.656
MOD_GSK3_1	32	39	PF00069	0.663
MOD_GSK3_1	355	362	PF00069	0.513
MOD_GSK3_1	457	464	PF00069	0.496
MOD_GSK3_1	550	557	PF00069	0.448
MOD_GSK3_1	569	576	PF00069	0.416
MOD_GSK3_1	601	608	PF00069	0.519
MOD_GSK3_1	635	642	PF00069	0.525
MOD_GSK3_1	689	696	PF00069	0.548
MOD_GSK3_1	78	85	PF00069	0.786
MOD_GSK3_1	801	808	PF00069	0.513
MOD_GSK3_1	99	106	PF00069	0.754
MOD_N-GLC_1	320	325	PF02516	0.210
MOD_NEK2_1	113	118	PF00069	0.677
MOD_NEK2_1	125	130	PF00069	0.702
MOD_NEK2_1	139	144	PF00069	0.744
MOD_NEK2_1	153	158	PF00069	0.527
MOD_NEK2_1	191	196	PF00069	0.532
MOD_NEK2_1	21	26	PF00069	0.656
MOD_NEK2_1	307	312	PF00069	0.498
MOD_NEK2_1	605	610	PF00069	0.502
MOD_NEK2_1	628	633	PF00069	0.487
MOD_NEK2_1	689	694	PF00069	0.535
MOD_NEK2_1	841	846	PF00069	0.576
MOD_NEK2_2	612	617	PF00069	0.487
MOD_PIKK_1	210	216	PF00454	0.519
MOD_PIKK_1	307	313	PF00454	0.487
MOD_PIKK_1	341	347	PF00454	0.410
MOD_PIKK_1	444	450	PF00454	0.538
MOD_PIKK_1	457	463	PF00454	0.412
MOD_PIKK_1	605	611	PF00454	0.512
MOD_PIKK_1	61	67	PF00454	0.631
MOD_PIKK_1	626	632	PF00454	0.487
MOD_PIKK_1	708	714	PF00454	0.589
MOD_PKA_1	97	103	PF00069	0.507
MOD_PKA_2	113	119	PF00069	0.586
MOD_PKA_2	217	223	PF00069	0.665
MOD_PKA_2	44	50	PF00069	0.686
MOD_PKA_2	480	486	PF00069	0.498
MOD_PKA_2	506	512	PF00069	0.487
MOD_PKA_2	513	519	PF00069	0.487
MOD_PKA_2	569	575	PF00069	0.478
MOD_PKA_2	720	726	PF00069	0.410
MOD_PKA_2	97	103	PF00069	0.582
MOD_PKB_1	475	483	PF00069	0.487
MOD_Plk_1	348	354	PF00069	0.449
MOD_Plk_1	359	365	PF00069	0.440
MOD_Plk_1	554	560	PF00069	0.564
MOD_Plk_1	665	671	PF00069	0.487
MOD_Plk_1	801	807	PF00069	0.464
MOD_Plk_1	841	847	PF00069	0.589
MOD_Plk_1	92	98	PF00069	0.690
MOD_Plk_2-3	675	681	PF00069	0.555
MOD_Plk_4	149	155	PF00069	0.554
MOD_Plk_4	201	207	PF00069	0.704
MOD_Plk_4	236	242	PF00069	0.507
MOD_Plk_4	297	303	PF00069	0.557
MOD_Plk_4	480	486	PF00069	0.569
MOD_Plk_4	547	553	PF00069	0.547
MOD_Plk_4	573	579	PF00069	0.548
MOD_Plk_4	601	607	PF00069	0.487
MOD_Plk_4	612	618	PF00069	0.487
MOD_Plk_4	689	695	PF00069	0.538
MOD_Plk_4	790	796	PF00069	0.487
MOD_Plk_4	842	848	PF00069	0.499
MOD_ProDKin_1	106	112	PF00069	0.701
MOD_ProDKin_1	156	162	PF00069	0.616
MOD_ProDKin_1	271	277	PF00069	0.492
MOD_ProDKin_1	598	604	PF00069	0.498
MOD_ProDKin_1	726	732	PF00069	0.565
MOD_ProDKin_1	82	88	PF00069	0.758
MOD_SUMO_for_1	422	425	PF00179	0.746
MOD_SUMO_for_1	523	526	PF00179	0.519
MOD_SUMO_rev_2	272	281	PF00179	0.488
TRG_DiLeu_BaLyEn_6	866	871	PF01217	0.528
TRG_ENDOCYTIC_2	195	198	PF00928	0.636
TRG_ENDOCYTIC_2	538	541	PF00928	0.403
TRG_ENDOCYTIC_2	597	600	PF00928	0.559
TRG_ENDOCYTIC_2	647	650	PF00928	0.487
TRG_ENDOCYTIC_2	829	832	PF00928	0.501
TRG_ER_diArg_1	173	176	PF00400	0.740
TRG_ER_diArg_1	379	381	PF00400	0.573
TRG_ER_diArg_1	595	597	PF00400	0.544
TRG_ER_diArg_1	69	71	PF00400	0.654
TRG_ER_diArg_1	734	736	PF00400	0.488
TRG_ER_diArg_1	853	855	PF00400	0.569
TRG_ER_diArg_1	96	98	PF00400	0.516
TRG_Pf-PMV_PEXEL_1	662	666	PF00026	0.287

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IMR3	Leptomonas seymouri	77%	100%
A0A0S4JDH2	Bodo saltans	61%	100%
A0A1X0P9Y4	Trypanosomatidae	64%	100%
A0A3R7P1H1	Trypanosoma rangeli	62%	100%
A0A3S7WWM6	Leishmania donovani	26%	100%
A0A3S7X7X7	Leishmania donovani	76%	100%
A4HBV0	Leishmania braziliensis	24%	100%
A4IA13	Leishmania infantum	76%	100%
C9ZMM2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	63%	100%
E9AGV6	Leishmania infantum	26%	100%
E9B520	Leishmania mexicana (strain MHOM/GT/2001/U1103)	77%	100%
Q4Q2W6	Leishmania major	76%	99%
Q4QCA9	Leishmania major	24%	100%
Q554D9	Dictyostelium discoideum	38%	100%
V5DGS5	Trypanosoma cruzi	62%	100%