Putative G-actin binding protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

twinfilin

Species:

Leishmania braziliensis

UniProt:

A4HAU1_LEIBR

TriTrypDb:

LbrM.20.1790 , LBRM2903_200028500

Length:

331

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005856	cytoskeleton	5	11
GO:0043226	organelle	2	11
GO:0043228	non-membrane-bounded organelle	3	11
GO:0043229	intracellular organelle	3	11
GO:0043232	intracellular non-membrane-bounded organelle	4	11
GO:0110165	cellular anatomical entity	1	12
GO:0005737	cytoplasm	2	1
GO:0005884	actin filament	6	1
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	1
GO:0097014	ciliary plasm	5	1
GO:0099080	supramolecular complex	2	1
GO:0099081	supramolecular polymer	3	1
GO:0099512	supramolecular fiber	4	1
GO:0099513	polymeric cytoskeletal fiber	5	1
GO:0099568	cytoplasmic region	3	1

Expansion

Sequence features

A4HAU1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HAU1

Function

Biological processes
Term	Name	Level	Count
GO:0008064	regulation of actin polymerization or depolymerization	6	12
GO:0009987	cellular process	1	12
GO:0010639	negative regulation of organelle organization	6	12
GO:0016043	cellular component organization	3	12
GO:0030832	regulation of actin filament length	5	12
GO:0030833	regulation of actin filament polymerization	7	12
GO:0030837	negative regulation of actin filament polymerization	7	12
GO:0031333	negative regulation of protein-containing complex assembly	6	12
GO:0032271	regulation of protein polymerization	6	12
GO:0032272	negative regulation of protein polymerization	7	12
GO:0032535	regulation of cellular component size	4	12
GO:0032956	regulation of actin cytoskeleton organization	5	12
GO:0032970	regulation of actin filament-based process	4	12
GO:0033043	regulation of organelle organization	5	12
GO:0043254	regulation of protein-containing complex assembly	5	12
GO:0044087	regulation of cellular component biogenesis	4	12
GO:0048519	negative regulation of biological process	3	12
GO:0048523	negative regulation of cellular process	4	12
GO:0050789	regulation of biological process	2	12
GO:0050794	regulation of cellular process	3	12
GO:0051128	regulation of cellular component organization	4	12
GO:0051129	negative regulation of cellular component organization	5	12
GO:0051493	regulation of cytoskeleton organization	6	12
GO:0051494	negative regulation of cytoskeleton organization	7	12
GO:0065007	biological regulation	1	12
GO:0065008	regulation of biological quality	2	12
GO:0071840	cellular component organization or biogenesis	2	12
GO:0090066	regulation of anatomical structure size	3	12
GO:0110053	regulation of actin filament organization	6	12
GO:1902903	regulation of supramolecular fiber organization	5	12
GO:1902904	negative regulation of supramolecular fiber organization	6	12
GO:0007015	actin filament organization	5	1
GO:0008154	actin polymerization or depolymerization	6	1
GO:0022411	cellular component disassembly	4	1
GO:0030042	actin filament depolymerization	7	1
GO:0030834	regulation of actin filament depolymerization	7	1
GO:0030835	negative regulation of actin filament depolymerization	7	1
GO:0032507	maintenance of protein location in cell	3	1
GO:0032984	protein-containing complex disassembly	5	1
GO:0042989	sequestering of actin monomers	4	1
GO:0043242	negative regulation of protein-containing complex disassembly	6	1
GO:0043244	regulation of protein-containing complex disassembly	5	1
GO:0043933	protein-containing complex organization	4	1
GO:0045185	maintenance of protein location	3	1
GO:0051016	barbed-end actin filament capping	9	1
GO:0051179	localization	1	1
GO:0051235	maintenance of location	2	1
GO:0051261	protein depolymerization	6	1
GO:0051651	maintenance of location in cell	2	1
GO:0051693	actin filament capping	8	1
GO:0097435	supramolecular fiber organization	4	1
GO:1901879	regulation of protein depolymerization	6	1
GO:1901880	negative regulation of protein depolymerization	7	1

Molecular functions
Term	Name	Level	Count
GO:0003779	actin binding	4	12
GO:0005488	binding	1	12
GO:0005515	protein binding	2	12
GO:0008092	cytoskeletal protein binding	3	12
GO:0003785	actin monomer binding	5	1
GO:0044877	protein-containing complex binding	2	1
GO:0051015	actin filament binding	3	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	61	65	PF00656	0.292
CLV_PCSK_SKI1_1	218	222	PF00082	0.283
CLV_PCSK_SKI1_1	227	231	PF00082	0.286
CLV_PCSK_SKI1_1	35	39	PF00082	0.458
CLV_PCSK_SKI1_1	60	64	PF00082	0.549
DEG_Nend_Nbox_1	1	3	PF02207	0.490
DEG_SCF_FBW7_1	171	178	PF00400	0.281
DOC_CYCLIN_yCln2_LP_2	41	47	PF00134	0.385
DOC_MAPK_DCC_7	156	166	PF00069	0.551
DOC_MAPK_gen_1	237	246	PF00069	0.442
DOC_MAPK_MEF2A_6	240	248	PF00069	0.456
DOC_PP1_RVXF_1	274	280	PF00149	0.379
DOC_PP2B_LxvP_1	40	43	PF13499	0.485
DOC_PP2B_PxIxI_1	254	260	PF00149	0.491
DOC_USP7_MATH_1	175	179	PF00917	0.464
DOC_USP7_MATH_1	301	305	PF00917	0.502
DOC_USP7_MATH_1	315	319	PF00917	0.519
DOC_USP7_UBL2_3	278	282	PF12436	0.483
DOC_WW_Pin1_4	108	113	PF00397	0.538
DOC_WW_Pin1_4	171	176	PF00397	0.328
DOC_WW_Pin1_4	232	237	PF00397	0.491
DOC_WW_Pin1_4	251	256	PF00397	0.539
DOC_WW_Pin1_4	284	289	PF00397	0.521
LIG_14-3-3_CanoR_1	97	107	PF00244	0.464
LIG_Clathr_ClatBox_1	30	34	PF01394	0.348
LIG_DCNL_PONY_1	1	4	PF03556	0.577
LIG_EVH1_2	43	47	PF00568	0.288
LIG_FHA_1	116	122	PF00498	0.463
LIG_FHA_1	191	197	PF00498	0.451
LIG_FHA_1	22	28	PF00498	0.449
LIG_FHA_1	79	85	PF00498	0.417
LIG_FHA_2	143	149	PF00498	0.415
LIG_FHA_2	220	226	PF00498	0.491
LIG_FHA_2	288	294	PF00498	0.533
LIG_FHA_2	59	65	PF00498	0.510
LIG_LIR_Gen_1	213	221	PF02991	0.537
LIG_LIR_Gen_1	225	236	PF02991	0.520
LIG_LIR_Gen_1	69	79	PF02991	0.343
LIG_LIR_Nem_3	213	217	PF02991	0.537
LIG_LIR_Nem_3	225	231	PF02991	0.520
LIG_LIR_Nem_3	297	302	PF02991	0.556
LIG_LIR_Nem_3	69	75	PF02991	0.326
LIG_PCNA_yPIPBox_3	195	207	PF02747	0.379
LIG_SH2_PTP2	247	250	PF00017	0.462
LIG_SH2_PTP2	72	75	PF00017	0.382
LIG_SH2_STAP1	261	265	PF00017	0.539
LIG_SH2_STAT5	247	250	PF00017	0.453
LIG_SH2_STAT5	72	75	PF00017	0.382
LIG_SH2_STAT5	87	90	PF00017	0.423
LIG_SH3_3	155	161	PF00018	0.506
LIG_SH3_3	245	251	PF00018	0.504
LIG_SUMO_SIM_anti_2	24	32	PF11976	0.311
LIG_SUMO_SIM_par_1	29	34	PF11976	0.377
LIG_TRAF2_1	15	18	PF00917	0.452
LIG_UBA3_1	30	35	PF00899	0.252
LIG_WRC_WIRS_1	191	196	PF05994	0.491
MOD_CDK_SPK_2	232	237	PF00069	0.491
MOD_CDK_SPK_2	251	256	PF00069	0.539
MOD_CDK_SPxxK_3	251	258	PF00069	0.539
MOD_CK1_1	219	225	PF00069	0.545
MOD_CK1_1	235	241	PF00069	0.448
MOD_CK1_1	316	322	PF00069	0.510
MOD_CK1_1	77	83	PF00069	0.398
MOD_CK2_1	103	109	PF00069	0.408
MOD_CK2_1	120	126	PF00069	0.424
MOD_CK2_1	127	133	PF00069	0.395
MOD_CK2_1	142	148	PF00069	0.368
MOD_CK2_1	287	293	PF00069	0.490
MOD_CK2_1	294	300	PF00069	0.515
MOD_GlcNHglycan	17	21	PF01048	0.479
MOD_GlcNHglycan	296	299	PF01048	0.463
MOD_GlcNHglycan	303	306	PF01048	0.601
MOD_GlcNHglycan	321	324	PF01048	0.715
MOD_GlcNHglycan	47	50	PF01048	0.458
MOD_GlcNHglycan	68	71	PF01048	0.354
MOD_GlcNHglycan	76	79	PF01048	0.434
MOD_GlcNHglycan	90	93	PF01048	0.455
MOD_GSK3_1	115	122	PF00069	0.406
MOD_GSK3_1	171	178	PF00069	0.454
MOD_GSK3_1	282	289	PF00069	0.516
MOD_GSK3_1	315	322	PF00069	0.584
MOD_GSK3_1	54	61	PF00069	0.416
MOD_GSK3_1	74	81	PF00069	0.491
MOD_GSK3_1	99	106	PF00069	0.520
MOD_N-GLC_1	22	27	PF02516	0.381
MOD_NEK2_1	16	21	PF00069	0.457
MOD_NEK2_1	56	61	PF00069	0.536
MOD_NEK2_1	98	103	PF00069	0.405
MOD_PIKK_1	179	185	PF00454	0.483
MOD_PIKK_1	235	241	PF00454	0.440
MOD_PIKK_1	78	84	PF00454	0.490
MOD_Plk_1	22	28	PF00069	0.255
MOD_Plk_1	4	10	PF00069	0.343
MOD_Plk_2-3	142	148	PF00069	0.351
MOD_Plk_4	127	133	PF00069	0.368
MOD_Plk_4	216	222	PF00069	0.506
MOD_Plk_4	287	293	PF00069	0.497
MOD_ProDKin_1	108	114	PF00069	0.540
MOD_ProDKin_1	171	177	PF00069	0.319
MOD_ProDKin_1	232	238	PF00069	0.491
MOD_ProDKin_1	251	257	PF00069	0.539
MOD_ProDKin_1	284	290	PF00069	0.521
MOD_SUMO_for_1	194	197	PF00179	0.507
TRG_DiLeu_BaEn_1	36	41	PF01217	0.474
TRG_ENDOCYTIC_2	72	75	PF00928	0.449

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P8Y3	Leptomonas seymouri	64%	100%
A0A0S4JJ86	Bodo saltans	27%	100%
A0A1X0P9W3	Trypanosomatidae	33%	97%
A0A3R7M8Y8	Trypanosoma rangeli	31%	100%
A0A3S7X7X8	Leishmania donovani	75%	100%
A4IA09	Leishmania infantum	75%	100%
C9ZMM6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	28%	100%
E9B515	Leishmania mexicana (strain MHOM/GT/2001/U1103)	76%	100%
Q4Q2X1	Leishmania major	74%	100%
V5BPY6	Trypanosoma cruzi	31%	100%