DNA-(apurinic or apyrimidinic site) lyase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

8-oxoguanine DNA glycosylase, putative

Species:

Leishmania braziliensis

UniProt:

A4HAS9_LEIBR

TriTrypDb:

LbrM.20.1670 , LBRM2903_200027300

Length:

569

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4HAS9

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HAS9

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	11
GO:0006259	DNA metabolic process	4	11
GO:0006281	DNA repair	5	11
GO:0006284	base-excision repair	6	10
GO:0006289	nucleotide-excision repair	6	11
GO:0006725	cellular aromatic compound metabolic process	3	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0006950	response to stress	2	11
GO:0006974	DNA damage response	4	11
GO:0008152	metabolic process	1	11
GO:0009987	cellular process	1	11
GO:0033554	cellular response to stress	3	11
GO:0034641	cellular nitrogen compound metabolic process	3	11
GO:0043170	macromolecule metabolic process	3	11
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0044260	obsolete cellular macromolecule metabolic process	3	11
GO:0046483	heterocycle metabolic process	3	11
GO:0050896	response to stimulus	1	11
GO:0051716	cellular response to stimulus	2	11
GO:0071704	organic substance metabolic process	2	11
GO:0090304	nucleic acid metabolic process	4	11
GO:1901360	organic cyclic compound metabolic process	3	11
GO:0006285	base-excision repair, AP site formation	5	1

Molecular functions
Term	Name	Level	Count
GO:0000702	oxidized base lesion DNA N-glycosylase activity	5	11
GO:0003676	nucleic acid binding	3	11
GO:0003677	DNA binding	4	11
GO:0003684	damaged DNA binding	5	11
GO:0003824	catalytic activity	1	11
GO:0003906	DNA-(apurinic or apyrimidinic site) endonuclease activity	4	11
GO:0005488	binding	1	11
GO:0008534	oxidized purine nucleobase lesion DNA N-glycosylase activity	6	11
GO:0016787	hydrolase activity	2	11
GO:0016798	hydrolase activity, acting on glycosyl bonds	3	11
GO:0016799	hydrolase activity, hydrolyzing N-glycosyl compounds	4	11
GO:0016829	lyase activity	2	11
GO:0016835	carbon-oxygen lyase activity	3	11
GO:0019104	DNA N-glycosylase activity	4	11
GO:0097159	organic cyclic compound binding	2	11
GO:0140078	class I DNA-(apurinic or apyrimidinic site) endonuclease activity	4	11
GO:0140097	catalytic activity, acting on DNA	3	11
GO:0140640	catalytic activity, acting on a nucleic acid	2	11
GO:1901363	heterocyclic compound binding	2	11
GO:0034039	8-oxo-7,8-dihydroguanine DNA N-glycosylase activity	7	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	316	320	PF00656	0.471
CLV_C14_Caspase3-7	370	374	PF00656	0.405
CLV_NRD_NRD_1	118	120	PF00675	0.364
CLV_NRD_NRD_1	184	186	PF00675	0.339
CLV_NRD_NRD_1	295	297	PF00675	0.321
CLV_NRD_NRD_1	425	427	PF00675	0.315
CLV_NRD_NRD_1	467	469	PF00675	0.614
CLV_NRD_NRD_1	492	494	PF00675	0.497
CLV_NRD_NRD_1	527	529	PF00675	0.743
CLV_NRD_NRD_1	89	91	PF00675	0.362
CLV_PCSK_KEX2_1	118	120	PF00082	0.386
CLV_PCSK_KEX2_1	295	297	PF00082	0.388
CLV_PCSK_KEX2_1	469	471	PF00082	0.623
CLV_PCSK_KEX2_1	88	90	PF00082	0.335
CLV_PCSK_PC1ET2_1	469	471	PF00082	0.623
CLV_PCSK_PC1ET2_1	88	90	PF00082	0.306
CLV_PCSK_SKI1_1	16	20	PF00082	0.588
CLV_PCSK_SKI1_1	415	419	PF00082	0.376
DEG_APCC_DBOX_1	196	204	PF00400	0.405
DEG_APCC_DBOX_1	414	422	PF00400	0.482
DEG_SCF_FBW7_1	134	141	PF00400	0.405
DEG_SCF_FBW7_1	275	281	PF00400	0.405
DEG_SCF_FBW7_1	42	47	PF00400	0.492
DEG_SIAH_1	459	467	PF03145	0.466
DEG_SPOP_SBC_1	17	21	PF00917	0.574
DEG_SPOP_SBC_1	505	509	PF00917	0.467
DOC_CKS1_1	135	140	PF01111	0.405
DOC_CKS1_1	275	280	PF01111	0.405
DOC_CKS1_1	41	46	PF01111	0.558
DOC_CYCLIN_yClb5_NLxxxL_5	25	32	PF00134	0.405
DOC_MAPK_gen_1	23	32	PF00069	0.583
DOC_MAPK_gen_1	426	434	PF00069	0.509
DOC_MAPK_gen_1	468	478	PF00069	0.507
DOC_MAPK_JIP1_4	472	478	PF00069	0.507
DOC_MAPK_MEF2A_6	216	225	PF00069	0.405
DOC_MAPK_MEF2A_6	23	32	PF00069	0.586
DOC_MAPK_NFAT4_5	25	33	PF00069	0.405
DOC_PP1_RVXF_1	342	349	PF00149	0.482
DOC_PP2B_LxvP_1	419	422	PF13499	0.496
DOC_PP4_MxPP_1	381	384	PF00568	0.405
DOC_USP7_MATH_1	138	142	PF00917	0.571
DOC_USP7_MATH_1	217	221	PF00917	0.405
DOC_USP7_MATH_1	278	282	PF00917	0.550
DOC_USP7_MATH_1	307	311	PF00917	0.562
DOC_USP7_MATH_1	313	317	PF00917	0.551
DOC_USP7_MATH_1	524	528	PF00917	0.726
DOC_USP7_MATH_1	97	101	PF00917	0.524
DOC_WW_Pin1_4	134	139	PF00397	0.568
DOC_WW_Pin1_4	178	183	PF00397	0.585
DOC_WW_Pin1_4	274	279	PF00397	0.518
DOC_WW_Pin1_4	305	310	PF00397	0.558
DOC_WW_Pin1_4	363	368	PF00397	0.514
DOC_WW_Pin1_4	40	45	PF00397	0.496
DOC_WW_Pin1_4	456	461	PF00397	0.492
DOC_WW_Pin1_4	527	532	PF00397	0.668
LIG_14-3-3_CanoR_1	16	26	PF00244	0.559
LIG_14-3-3_CanoR_1	45	49	PF00244	0.461
LIG_14-3-3_CanoR_1	518	523	PF00244	0.746
LIG_Actin_WH2_1	45	62	PF00022	0.586
LIG_Actin_WH2_2	44	62	PF00022	0.475
LIG_BIR_III_4	319	323	PF00653	0.405
LIG_BRCT_BRCA1_1	106	110	PF00533	0.535
LIG_BRCT_BRCA1_1	233	237	PF00533	0.562
LIG_BRCT_BRCA1_1	559	563	PF00533	0.364
LIG_CtBP_PxDLS_1	148	152	PF00389	0.405
LIG_deltaCOP1_diTrp_1	172	178	PF00928	0.562
LIG_FHA_1	17	23	PF00498	0.556
LIG_FHA_1	249	255	PF00498	0.499
LIG_FHA_1	270	276	PF00498	0.471
LIG_FHA_1	338	344	PF00498	0.590
LIG_FHA_1	438	444	PF00498	0.551
LIG_FHA_2	288	294	PF00498	0.525
LIG_LIR_Apic_2	325	330	PF02991	0.562
LIG_LIR_Gen_1	162	171	PF02991	0.562
LIG_LIR_Gen_1	220	228	PF02991	0.405
LIG_LIR_Gen_1	545	555	PF02991	0.287
LIG_LIR_Nem_3	111	117	PF02991	0.524
LIG_LIR_Nem_3	162	167	PF02991	0.562
LIG_LIR_Nem_3	220	225	PF02991	0.519
LIG_LIR_Nem_3	234	240	PF02991	0.547
LIG_LIR_Nem_3	328	332	PF02991	0.506
LIG_LIR_Nem_3	47	51	PF02991	0.457
LIG_LIR_Nem_3	545	550	PF02991	0.553
LIG_MYND_1	309	313	PF01753	0.502
LIG_MYND_2	2	6	PF01753	0.530
LIG_NRBOX	199	205	PF00104	0.405
LIG_Pex14_1	323	327	PF04695	0.562
LIG_Pex14_2	110	114	PF04695	0.433
LIG_Pex14_2	547	551	PF04695	0.392
LIG_PTB_Apo_2	394	401	PF02174	0.586
LIG_PTB_Phospho_1	394	400	PF10480	0.586
LIG_SH2_CRK	201	205	PF00017	0.405
LIG_SH2_CRK	327	331	PF00017	0.586
LIG_SH2_CRK	400	404	PF00017	0.586
LIG_SH2_PTP2	222	225	PF00017	0.562
LIG_SH2_STAP1	400	404	PF00017	0.485
LIG_SH2_STAT3	261	264	PF00017	0.405
LIG_SH2_STAT5	164	167	PF00017	0.482
LIG_SH2_STAT5	192	195	PF00017	0.513
LIG_SH2_STAT5	222	225	PF00017	0.562
LIG_SH2_STAT5	354	357	PF00017	0.475
LIG_SH2_STAT5	456	459	PF00017	0.450
LIG_SH3_1	528	534	PF00018	0.635
LIG_SH3_3	272	278	PF00018	0.562
LIG_SH3_3	528	534	PF00018	0.594
LIG_SUMO_SIM_anti_2	416	422	PF11976	0.586
LIG_TRAF2_1	152	155	PF00917	0.564
LIG_TRAF2_1	156	159	PF00917	0.560
LIG_TRAF2_1	367	370	PF00917	0.405
LIG_TYR_ITIM	115	120	PF00017	0.562
LIG_WRC_WIRS_1	236	241	PF05994	0.447
LIG_WW_1	533	536	PF00397	0.586
LIG_WW_3	522	526	PF00397	0.582
MOD_CDK_SPK_2	40	45	PF00069	0.496
MOD_CDK_SPK_2	456	461	PF00069	0.612
MOD_CDK_SPxK_1	274	280	PF00069	0.518
MOD_CDK_SPxxK_3	178	185	PF00069	0.586
MOD_CDK_SPxxK_3	305	312	PF00069	0.558
MOD_CK1_1	136	142	PF00069	0.553
MOD_CK1_1	181	187	PF00069	0.586
MOD_CK1_1	202	208	PF00069	0.405
MOD_CK1_1	325	331	PF00069	0.542
MOD_CK1_1	336	342	PF00069	0.506
MOD_CK1_1	506	512	PF00069	0.746
MOD_CK1_1	527	533	PF00069	0.614
MOD_CK2_1	153	159	PF00069	0.541
MOD_CK2_1	280	286	PF00069	0.513
MOD_CK2_1	287	293	PF00069	0.469
MOD_CK2_1	52	58	PF00069	0.486
MOD_CMANNOS	175	178	PF00535	0.282
MOD_Cter_Amidation	424	427	PF01082	0.282
MOD_Cter_Amidation	466	469	PF01082	0.740
MOD_Cter_Amidation	86	89	PF01082	0.205
MOD_GlcNHglycan	280	283	PF01048	0.386
MOD_GlcNHglycan	335	338	PF01048	0.211
MOD_GlcNHglycan	518	521	PF01048	0.799
MOD_GlcNHglycan	6	9	PF01048	0.637
MOD_GlcNHglycan	94	97	PF01048	0.382
MOD_GSK3_1	104	111	PF00069	0.481
MOD_GSK3_1	133	140	PF00069	0.570
MOD_GSK3_1	159	166	PF00069	0.508
MOD_GSK3_1	217	224	PF00069	0.574
MOD_GSK3_1	231	238	PF00069	0.556
MOD_GSK3_1	274	281	PF00069	0.534
MOD_GSK3_1	333	340	PF00069	0.567
MOD_GSK3_1	40	47	PF00069	0.426
MOD_GSK3_1	505	512	PF00069	0.487
MOD_GSK3_1	523	530	PF00069	0.553
MOD_N-GLC_1	104	109	PF02516	0.313
MOD_N-GLC_1	245	250	PF02516	0.362
MOD_N-GLC_1	437	442	PF02516	0.386
MOD_N-GLC_1	52	57	PF02516	0.367
MOD_NEK2_1	142	147	PF00069	0.519
MOD_NEK2_1	18	23	PF00069	0.550
MOD_NEK2_1	221	226	PF00069	0.479
MOD_NEK2_1	239	244	PF00069	0.468
MOD_NEK2_1	28	33	PF00069	0.519
MOD_NEK2_1	361	366	PF00069	0.589
MOD_NEK2_1	399	404	PF00069	0.463
MOD_NEK2_1	437	442	PF00069	0.569
MOD_NEK2_1	52	57	PF00069	0.465
MOD_NEK2_2	217	222	PF00069	0.405
MOD_NEK2_2	322	327	PF00069	0.405
MOD_PIKK_1	239	245	PF00454	0.482
MOD_PIKK_1	361	367	PF00454	0.567
MOD_PIKK_1	76	82	PF00454	0.502
MOD_PKA_1	295	301	PF00069	0.468
MOD_PKA_1	468	474	PF00069	0.644
MOD_PKA_2	248	254	PF00069	0.496
MOD_PKA_2	295	301	PF00069	0.572
MOD_PKA_2	314	320	PF00069	0.391
MOD_PKA_2	44	50	PF00069	0.484
MOD_PKA_2	524	530	PF00069	0.756
MOD_Plk_1	245	251	PF00069	0.562
MOD_Plk_1	437	443	PF00069	0.591
MOD_Plk_1	52	58	PF00069	0.405
MOD_Plk_4	138	144	PF00069	0.574
MOD_Plk_4	199	205	PF00069	0.536
MOD_Plk_4	217	223	PF00069	0.444
MOD_Plk_4	322	328	PF00069	0.562
MOD_Plk_4	399	405	PF00069	0.492
MOD_Plk_4	44	50	PF00069	0.482
MOD_Plk_4	52	58	PF00069	0.482
MOD_Plk_4	557	563	PF00069	0.361
MOD_ProDKin_1	134	140	PF00069	0.568
MOD_ProDKin_1	178	184	PF00069	0.585
MOD_ProDKin_1	274	280	PF00069	0.518
MOD_ProDKin_1	305	311	PF00069	0.558
MOD_ProDKin_1	363	369	PF00069	0.514
MOD_ProDKin_1	40	46	PF00069	0.496
MOD_ProDKin_1	456	462	PF00069	0.508
MOD_ProDKin_1	527	533	PF00069	0.656
MOD_SUMO_rev_2	281	290	PF00179	0.522
TRG_ENDOCYTIC_2	117	120	PF00928	0.570
TRG_ENDOCYTIC_2	164	167	PF00928	0.482
TRG_ENDOCYTIC_2	201	204	PF00928	0.526
TRG_ENDOCYTIC_2	222	225	PF00928	0.562
TRG_ENDOCYTIC_2	400	403	PF00928	0.482
TRG_ENDOCYTIC_2	564	567	PF00928	0.411
TRG_ER_diArg_1	117	119	PF00400	0.593
TRG_ER_diArg_1	294	296	PF00400	0.423
TRG_ER_diArg_1	470	473	PF00400	0.668
TRG_NES_CRM1_1	159	172	PF08389	0.586
TRG_NLS_MonoExtC_3	467	472	PF00514	0.656
TRG_NLS_MonoExtN_4	465	472	PF00514	0.617

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I223	Leptomonas seymouri	43%	97%
A0A1X0P9L2	Trypanosomatidae	29%	100%
A0A3R7MS38	Trypanosoma rangeli	33%	100%
A0A3S7X7Y5	Leishmania donovani	69%	100%
A4I9Y9	Leishmania infantum	69%	100%
C9ZMN9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	33%	100%
E9B503	Leishmania mexicana (strain MHOM/GT/2001/U1103)	69%	100%
Q4Q2Y3	Leishmania major	70%	100%
V5BKI1	Trypanosoma cruzi	31%	100%