Putative ubiquitin-conjugating enzyme e2

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ubiquitin-conjugating enzyme E2, putative

Species:

Leishmania braziliensis

UniProt:

A4HAK6_LEIBR

TriTrypDb:

LbrM.20.0870 , LBRM2903_200015700

Length:

290

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 9
NetGPI	no	yes: 0, no: 9

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0005783	endoplasmic reticulum	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	4
GO:0016020	membrane	2	3

Expansion

Sequence features

A4HAK6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HAK6

Function

Biological processes
Term	Name	Level	Count
GO:0000209	protein polyubiquitination	8	1
GO:0006508	proteolysis	4	1
GO:0006511	ubiquitin-dependent protein catabolic process	7	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0006950	response to stress	2	1
GO:0008152	metabolic process	1	1
GO:0009056	catabolic process	2	1
GO:0009057	macromolecule catabolic process	4	1
GO:0009987	cellular process	1	1
GO:0010033	response to organic substance	3	1
GO:0010243	response to organonitrogen compound	4	1
GO:0010498	proteasomal protein catabolic process	5	1
GO:0016567	protein ubiquitination	7	1
GO:0019538	protein metabolic process	3	1
GO:0019941	modification-dependent protein catabolic process	6	1
GO:0030163	protein catabolic process	4	1
GO:0030433	ubiquitin-dependent ERAD pathway	6	1
GO:0032446	protein modification by small protein conjugation	6	1
GO:0033554	cellular response to stress	3	1
GO:0034976	response to endoplasmic reticulum stress	4	1
GO:0036211	protein modification process	4	1
GO:0036503	ERAD pathway	5	1
GO:0042221	response to chemical	2	1
GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process	6	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0043632	modification-dependent macromolecule catabolic process	5	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044248	cellular catabolic process	3	1
GO:0044260	obsolete cellular macromolecule metabolic process	3	1
GO:0044265	obsolete cellular macromolecule catabolic process	4	1
GO:0050896	response to stimulus	1	1
GO:0051603	proteolysis involved in protein catabolic process	5	1
GO:0051716	cellular response to stimulus	2	1
GO:0070647	protein modification by small protein conjugation or removal	5	1
GO:0071704	organic substance metabolic process	2	1
GO:1901564	organonitrogen compound metabolic process	3	1
GO:1901565	organonitrogen compound catabolic process	4	1
GO:1901575	organic substance catabolic process	3	1
GO:1901698	response to nitrogen compound	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	9
GO:0016874	ligase activity	2	9
GO:0004842	ubiquitin-protein transferase activity	4	2
GO:0016740	transferase activity	2	2
GO:0019787	ubiquitin-like protein transferase activity	3	2
GO:0061631	ubiquitin conjugating enzyme activity	5	2
GO:0061650	ubiquitin-like protein conjugating enzyme activity	4	2
GO:0140096	catalytic activity, acting on a protein	2	2
GO:0016746	acyltransferase activity	3	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_MEL_PAP_1	25	31	PF00089	0.226
CLV_NRD_NRD_1	134	136	PF00675	0.271
CLV_NRD_NRD_1	141	143	PF00675	0.332
CLV_NRD_NRD_1	16	18	PF00675	0.295
CLV_NRD_NRD_1	248	250	PF00675	0.475
CLV_NRD_NRD_1	270	272	PF00675	0.381
CLV_PCSK_KEX2_1	10	12	PF00082	0.272
CLV_PCSK_KEX2_1	134	136	PF00082	0.272
CLV_PCSK_KEX2_1	147	149	PF00082	0.344
CLV_PCSK_KEX2_1	154	156	PF00082	0.207
CLV_PCSK_KEX2_1	248	250	PF00082	0.537
CLV_PCSK_KEX2_1	270	272	PF00082	0.381
CLV_PCSK_PC1ET2_1	10	12	PF00082	0.389
CLV_PCSK_PC1ET2_1	147	149	PF00082	0.461
CLV_PCSK_PC1ET2_1	154	156	PF00082	0.362
CLV_PCSK_SKI1_1	107	111	PF00082	0.314
CLV_PCSK_SKI1_1	154	158	PF00082	0.369
DEG_APCC_DBOX_1	106	114	PF00400	0.514
DOC_CKS1_1	75	80	PF01111	0.472
DOC_MAPK_gen_1	10	16	PF00069	0.514
DOC_MAPK_gen_1	270	278	PF00069	0.589
DOC_MAPK_gen_1	81	90	PF00069	0.589
DOC_MAPK_HePTP_8	267	279	PF00069	0.589
DOC_MAPK_MEF2A_6	270	279	PF00069	0.589
DOC_MAPK_MEF2A_6	81	90	PF00069	0.514
DOC_PP4_FxxP_1	68	71	PF00568	0.589
DOC_USP7_MATH_1	112	116	PF00917	0.589
DOC_USP7_MATH_1	138	142	PF00917	0.538
DOC_USP7_UBL2_3	143	147	PF12436	0.647
DOC_WW_Pin1_4	171	176	PF00397	0.763
DOC_WW_Pin1_4	221	226	PF00397	0.823
DOC_WW_Pin1_4	46	51	PF00397	0.472
DOC_WW_Pin1_4	74	79	PF00397	0.472
DOC_WW_Pin1_4	98	103	PF00397	0.501
LIG_14-3-3_CanoR_1	210	218	PF00244	0.683
LIG_14-3-3_CanoR_1	270	276	PF00244	0.589
LIG_deltaCOP1_diTrp_1	98	104	PF00928	0.472
LIG_DLG_GKlike_1	271	278	PF00625	0.485
LIG_FHA_1	106	112	PF00498	0.483
LIG_FHA_1	214	220	PF00498	0.681
LIG_FHA_2	128	134	PF00498	0.589
LIG_FHA_2	210	216	PF00498	0.630
LIG_FHA_2	47	53	PF00498	0.472
LIG_LIR_Apic_2	65	71	PF02991	0.589
LIG_PCNA_PIPBox_1	34	43	PF02747	0.589
LIG_PCNA_yPIPBox_3	28	41	PF02747	0.568
LIG_PTB_Apo_2	89	96	PF02174	0.589
LIG_PTB_Phospho_1	89	95	PF10480	0.589
LIG_SH2_CRK	153	157	PF00017	0.561
LIG_SH2_CRK	272	276	PF00017	0.485
LIG_SH2_PTP2	56	59	PF00017	0.589
LIG_SH2_STAP1	272	276	PF00017	0.485
LIG_SH2_STAT5	40	43	PF00017	0.499
LIG_SH2_STAT5	56	59	PF00017	0.472
LIG_SH3_3	40	46	PF00018	0.432
LIG_SH3_3	64	70	PF00018	0.589
LIG_SUMO_SIM_anti_2	274	279	PF11976	0.380
LIG_SUMO_SIM_par_1	108	115	PF11976	0.589
LIG_SUMO_SIM_par_1	58	65	PF11976	0.541
LIG_TRAF2_1	118	121	PF00917	0.488
LIG_TRAF2_1	130	133	PF00917	0.546
LIG_TRAF2_1	187	190	PF00917	0.693
LIG_TRAF2_1	206	209	PF00917	0.732
LIG_WRC_WIRS_1	113	118	PF05994	0.472
MOD_CDK_SPxxK_3	74	81	PF00069	0.472
MOD_CK1_1	181	187	PF00069	0.814
MOD_CK1_1	213	219	PF00069	0.816
MOD_CK1_1	91	97	PF00069	0.589
MOD_CK2_1	127	133	PF00069	0.589
MOD_CK2_1	182	188	PF00069	0.774
MOD_CK2_1	209	215	PF00069	0.619
MOD_CK2_1	46	52	PF00069	0.472
MOD_GlcNHglycan	127	130	PF01048	0.337
MOD_GlcNHglycan	184	187	PF01048	0.594
MOD_GlcNHglycan	256	259	PF01048	0.473
MOD_GlcNHglycan	78	81	PF01048	0.314
MOD_GSK3_1	112	119	PF00069	0.486
MOD_GSK3_1	121	128	PF00069	0.500
MOD_GSK3_1	177	184	PF00069	0.729
MOD_GSK3_1	209	216	PF00069	0.820
MOD_GSK3_1	232	239	PF00069	0.742
MOD_N-GLC_1	182	187	PF02516	0.528
MOD_N-GLC_1	254	259	PF02516	0.474
MOD_N-GLC_1	91	96	PF02516	0.337
MOD_N-GLC_1	98	103	PF02516	0.289
MOD_NEK2_1	90	95	PF00069	0.514
MOD_NEK2_2	112	117	PF00069	0.589
MOD_PK_1	69	75	PF00069	0.589
MOD_PKA_2	209	215	PF00069	0.684
MOD_Plk_1	91	97	PF00069	0.589
MOD_Plk_4	112	118	PF00069	0.472
MOD_Plk_4	232	238	PF00069	0.660
MOD_Plk_4	271	277	PF00069	0.362
MOD_ProDKin_1	171	177	PF00069	0.769
MOD_ProDKin_1	221	227	PF00069	0.825
MOD_ProDKin_1	46	52	PF00069	0.472
MOD_ProDKin_1	74	80	PF00069	0.472
MOD_ProDKin_1	98	104	PF00069	0.501
MOD_SUMO_for_1	201	204	PF00179	0.679
TRG_ENDOCYTIC_2	153	156	PF00928	0.522
TRG_ENDOCYTIC_2	272	275	PF00928	0.453
TRG_ENDOCYTIC_2	40	43	PF00928	0.472
TRG_ENDOCYTIC_2	56	59	PF00928	0.472
TRG_ER_diArg_1	247	249	PF00400	0.666
TRG_ER_diArg_1	269	271	PF00400	0.729
TRG_Pf-PMV_PEXEL_1	155	159	PF00026	0.448
TRG_Pf-PMV_PEXEL_1	17	22	PF00026	0.299

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A3Q8IHC0	Leishmania donovani	81%	100%
A0A3R7ND33	Trypanosoma rangeli	44%	100%
A4HAK3	Leishmania braziliensis	99%	100%
A4HAK5	Leishmania braziliensis	98%	100%
A4I9P7	Leishmania infantum	80%	100%
C9ZMW8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	39%	100%
E9B4R1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	81%	99%
Q4Q3B5	Leishmania major	81%	100%
Q6FQK7	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	37%	100%
Q941B6	Arabidopsis thaliana	27%	71%