Putative DNA-directed RNA polymerase subunit

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

DNA-directed rna polymerase II subunit, putative

Species:

Leishmania braziliensis

UniProt:

A4HAK2_LEIBR

TriTrypDb:

LbrM.20.0860 , LBRM2903_200015600

Length:

159

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0000428	DNA-directed RNA polymerase complex	4	11
GO:0005665	RNA polymerase II, core complex	4	9
GO:0030880	RNA polymerase complex	3	11
GO:0032991	protein-containing complex	1	11
GO:0055029	nuclear DNA-directed RNA polymerase complex	3	9
GO:0061695	transferase complex, transferring phosphorus-containing groups	4	11
GO:0140513	nuclear protein-containing complex	2	9
GO:0140535	intracellular protein-containing complex	2	11
GO:1902494	catalytic complex	2	11
GO:1990234	transferase complex	3	11
GO:0005654	nucleoplasm	2	1
GO:0005666	RNA polymerase III complex	4	1
GO:0005736	RNA polymerase I complex	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4HAK2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HAK2

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	11
GO:0006351	DNA-templated transcription	7	11
GO:0006725	cellular aromatic compound metabolic process	3	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0008152	metabolic process	1	11
GO:0009058	biosynthetic process	2	11
GO:0009059	macromolecule biosynthetic process	4	11
GO:0009987	cellular process	1	11
GO:0016070	RNA metabolic process	5	11
GO:0018130	heterocycle biosynthetic process	4	11
GO:0019438	aromatic compound biosynthetic process	4	11
GO:0032774	RNA biosynthetic process	5	11
GO:0034641	cellular nitrogen compound metabolic process	3	11
GO:0034654	nucleobase-containing compound biosynthetic process	4	11
GO:0043170	macromolecule metabolic process	3	11
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0044249	cellular biosynthetic process	3	11
GO:0044271	cellular nitrogen compound biosynthetic process	4	11
GO:0046483	heterocycle metabolic process	3	11
GO:0071704	organic substance metabolic process	2	11
GO:0090304	nucleic acid metabolic process	4	11
GO:0097659	nucleic acid-templated transcription	6	11
GO:1901360	organic cyclic compound metabolic process	3	11
GO:1901362	organic cyclic compound biosynthetic process	4	11
GO:1901576	organic substance biosynthetic process	3	11

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	11
GO:0003677	DNA binding	4	11
GO:0003824	catalytic activity	1	11
GO:0003899	DNA-directed 5'-3' RNA polymerase activity	6	11
GO:0005488	binding	1	11
GO:0016740	transferase activity	2	11
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	11
GO:0016779	nucleotidyltransferase activity	4	11
GO:0034062	5'-3' RNA polymerase activity	5	11
GO:0097159	organic cyclic compound binding	2	11
GO:0097747	RNA polymerase activity	4	11
GO:0140098	catalytic activity, acting on RNA	3	11
GO:0140640	catalytic activity, acting on a nucleic acid	2	11
GO:1901363	heterocyclic compound binding	2	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	121	123	PF00675	0.259
CLV_NRD_NRD_1	128	130	PF00675	0.330
CLV_PCSK_FUR_1	119	123	PF00082	0.336
CLV_PCSK_KEX2_1	121	123	PF00082	0.259
CLV_PCSK_KEX2_1	128	130	PF00082	0.330
CLV_PCSK_SKI1_1	128	132	PF00082	0.403
DEG_SPOP_SBC_1	22	26	PF00917	0.478
DOC_MAPK_gen_1	119	127	PF00069	0.440
DOC_MAPK_RevD_3	113	129	PF00069	0.512
DOC_PP4_FxxP_1	148	151	PF00568	0.430
DOC_WW_Pin1_4	120	125	PF00397	0.512
LIG_14-3-3_CanoR_1	72	81	PF00244	0.286
LIG_BIR_II_1	1	5	PF00653	0.592
LIG_Clathr_ClatBox_1	145	149	PF01394	0.521
LIG_FHA_1	106	112	PF00498	0.428
LIG_FHA_1	121	127	PF00498	0.407
LIG_FHA_1	48	54	PF00498	0.332
LIG_FHA_1	72	78	PF00498	0.286
LIG_FHA_1	87	93	PF00498	0.412
LIG_FHA_2	133	139	PF00498	0.365
LIG_FHA_2	77	83	PF00498	0.442
LIG_FXI_DFP_1	145	149	PF00024	0.521
LIG_LIR_Apic_2	147	151	PF02991	0.431
LIG_LIR_Gen_1	153	159	PF02991	0.492
LIG_LIR_Nem_3	153	159	PF02991	0.492
LIG_PDZ_Class_1	154	159	PF00595	0.496
LIG_SH2_GRB2like	156	159	PF00017	0.518
LIG_SH2_STAT5	156	159	PF00017	0.518
LIG_SUMO_SIM_par_1	111	117	PF11976	0.476
MOD_CK2_1	137	143	PF00069	0.504
MOD_CK2_1	29	35	PF00069	0.543
MOD_CK2_1	76	82	PF00069	0.433
MOD_GlcNHglycan	31	34	PF01048	0.743
MOD_GlcNHglycan	54	57	PF01048	0.453
MOD_GlcNHglycan	74	77	PF01048	0.100
MOD_GSK3_1	105	112	PF00069	0.433
MOD_GSK3_1	72	79	PF00069	0.359
MOD_N-GLC_1	22	27	PF02516	0.480
MOD_NEK2_1	92	97	PF00069	0.433
MOD_PKA_2	71	77	PF00069	0.305
MOD_Plk_1	132	138	PF00069	0.376
MOD_Plk_2-3	137	143	PF00069	0.504
MOD_ProDKin_1	120	126	PF00069	0.512
TRG_DiLeu_BaLyEn_6	121	126	PF01217	0.446
TRG_ENDOCYTIC_2	156	159	PF00928	0.518
TRG_ER_diArg_1	127	129	PF00400	0.361
TRG_ER_diArg_1	83	86	PF00400	0.433
TRG_Pf-PMV_PEXEL_1	116	120	PF00026	0.233
TRG_Pf-PMV_PEXEL_1	129	133	PF00026	0.389
TRG_Pf-PMV_PEXEL_1	67	71	PF00026	0.500

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P7X7	Leptomonas seymouri	33%	100%
A0A0N1HWY9	Leptomonas seymouri	82%	98%
A0A0S4JLU1	Bodo saltans	74%	100%
A0A1X0NEN4	Trypanosomatidae	60%	100%
A0A1X0P4L0	Trypanosomatidae	40%	100%
A0A3Q8IDA2	Leishmania donovani	33%	100%
A0A3Q8IKT1	Leishmania donovani	90%	100%
A0A422NF56	Trypanosoma rangeli	37%	100%
A4HDW1	Leishmania braziliensis	30%	100%
A4I156	Leishmania infantum	33%	100%
A4I9P6	Leishmania infantum	89%	100%
A8WSV7	Caenorhabditis briggsae	56%	100%
C9ZMZ5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	60%	100%
C9ZMZ7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	60%	100%
E9AX90	Leishmania mexicana (strain MHOM/GT/2001/U1103)	32%	100%
E9B4R0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	89%	100%
P20435	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	50%	100%
Q4Q3B6	Leishmania major	91%	100%
Q4QA82	Leishmania major	32%	100%