Putative ascorbate-dependent peroxidase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ascorbate peroxidase, putative

Species:

Leishmania braziliensis

UniProt:

A4HAD2_LEIBR

TriTrypDb:

LbrM.20.0150 , LBRM2903_200008300

Length:

305

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 20

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 16

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 9
NetGPI	no	yes: 0, no: 9

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4HAD2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HAD2

PDB structure(s): 3riv_A , 3riv_B , 3riw_A , 3riw_B , 4ged_A , 5al9_A , 5ala_A , 5ala_B , 5amm_A , 5amm_B

Function

Biological processes
Term	Name	Level	Count
GO:0006950	response to stress	2	10
GO:0006979	response to oxidative stress	3	10
GO:0009987	cellular process	1	10
GO:0033554	cellular response to stress	3	10
GO:0034599	cellular response to oxidative stress	4	10
GO:0042221	response to chemical	2	10
GO:0050896	response to stimulus	1	10
GO:0051716	cellular response to stimulus	2	10
GO:0062197	cellular response to chemical stress	4	10
GO:0070887	cellular response to chemical stimulus	3	10
GO:0000302	response to reactive oxygen species	4	1
GO:0008152	metabolic process	1	1
GO:0009056	catabolic process	2	1
GO:0010941	regulation of cell death	4	1
GO:0030322	stabilization of membrane potential	4	1
GO:0042391	regulation of membrane potential	3	1
GO:0042592	homeostatic process	3	1
GO:0042743	hydrogen peroxide metabolic process	4	1
GO:0042744	hydrogen peroxide catabolic process	4	1
GO:0042981	regulation of apoptotic process	6	1
GO:0043066	negative regulation of apoptotic process	7	1
GO:0043067	regulation of programmed cell death	5	1
GO:0043069	negative regulation of programmed cell death	6	1
GO:0044237	cellular metabolic process	2	1
GO:0044248	cellular catabolic process	3	1
GO:0048519	negative regulation of biological process	3	1
GO:0048523	negative regulation of cellular process	4	1
GO:0048878	chemical homeostasis	4	1
GO:0050789	regulation of biological process	2	1
GO:0050794	regulation of cellular process	3	1
GO:0050801	monoatomic ion homeostasis	5	1
GO:0055065	obsolete metal ion homeostasis	7	1
GO:0055074	calcium ion homeostasis	8	1
GO:0055080	monoatomic cation homeostasis	6	1
GO:0060548	negative regulation of cell death	5	1
GO:0065007	biological regulation	1	1
GO:0065008	regulation of biological quality	2	1
GO:0072507	obsolete divalent inorganic cation homeostasis	7	1
GO:0072593	reactive oxygen species metabolic process	3	1
GO:0098771	inorganic ion homeostasis	6	1
GO:1901700	response to oxygen-containing compound	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	10
GO:0004601	peroxidase activity	2	10
GO:0005488	binding	1	10
GO:0016209	antioxidant activity	1	10
GO:0016491	oxidoreductase activity	2	10
GO:0016684	oxidoreductase activity, acting on peroxide as acceptor	3	10
GO:0016688	L-ascorbate peroxidase activity	3	6
GO:0020037	heme binding	4	10
GO:0043167	ion binding	2	9
GO:0043169	cation binding	3	9
GO:0046872	metal ion binding	4	9
GO:0046906	tetrapyrrole binding	3	10
GO:0097159	organic cyclic compound binding	2	10
GO:1901363	heterocyclic compound binding	2	10
GO:0004129	cytochrome-c oxidase activity	4	1
GO:0004447	iodide peroxidase activity	4	1
GO:0005215	transporter activity	1	1
GO:0008324	monoatomic cation transmembrane transporter activity	4	1
GO:0008447	L-ascorbate oxidase activity	5	1
GO:0009055	electron transfer activity	3	1
GO:0015075	monoatomic ion transmembrane transporter activity	3	1
GO:0015078	proton transmembrane transporter activity	5	1
GO:0015318	inorganic molecular entity transmembrane transporter activity	3	1
GO:0015399	primary active transmembrane transporter activity	4	1
GO:0015453	oxidoreduction-driven active transmembrane transporter activity	5	1
GO:0016675	oxidoreductase activity, acting on a heme group of donors	3	1
GO:0016679	oxidoreductase activity, acting on diphenols and related substances as donors	3	1
GO:0016682	oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor	4	1
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen	3	1
GO:0022804	active transmembrane transporter activity	3	1
GO:0022853	active monoatomic ion transmembrane transporter activity	4	1
GO:0022857	transmembrane transporter activity	2	1
GO:0022890	inorganic cation transmembrane transporter activity	4	1
GO:0140905	haloperoxidase activity	3	1
GO:0140906	halogenase activity	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	150	154	PF00656	0.226
CLV_NRD_NRD_1	107	109	PF00675	0.471
CLV_NRD_NRD_1	264	266	PF00675	0.509
CLV_NRD_NRD_1	297	299	PF00675	0.447
CLV_NRD_NRD_1	6	8	PF00675	0.491
CLV_PCSK_KEX2_1	107	109	PF00082	0.486
CLV_PCSK_KEX2_1	6	8	PF00082	0.491
CLV_PCSK_SKI1_1	107	111	PF00082	0.426
CLV_PCSK_SKI1_1	174	178	PF00082	0.443
CLV_PCSK_SKI1_1	287	291	PF00082	0.524
CLV_PCSK_SKI1_1	6	10	PF00082	0.492
CLV_Separin_Metazoa	104	108	PF03568	0.185
DOC_MAPK_gen_1	263	270	PF00069	0.222
DOC_PP1_RVXF_1	177	183	PF00149	0.242
DOC_PP1_RVXF_1	64	70	PF00149	0.226
DOC_PP2B_LxvP_1	103	106	PF13499	0.309
DOC_PP4_FxxP_1	206	209	PF00568	0.172
DOC_USP7_MATH_1	16	20	PF00917	0.406
DOC_USP7_UBL2_3	295	299	PF12436	0.289
LIG_14-3-3_CanoR_1	244	252	PF00244	0.293
LIG_Actin_WH2_2	282	300	PF00022	0.302
LIG_BRCT_BRCA1_1	140	144	PF00533	0.168
LIG_BRCT_BRCA1_1	282	286	PF00533	0.202
LIG_BRCT_BRCA1_1	87	91	PF00533	0.247
LIG_BRCT_BRCA1_2	282	288	PF00533	0.206
LIG_deltaCOP1_diTrp_1	207	217	PF00928	0.185
LIG_FHA_1	185	191	PF00498	0.239
LIG_FHA_1	221	227	PF00498	0.306
LIG_FHA_1	244	250	PF00498	0.226
LIG_FHA_1	292	298	PF00498	0.313
LIG_FHA_2	193	199	PF00498	0.243
LIG_LIR_Apic_2	204	209	PF02991	0.226
LIG_LIR_Gen_1	121	130	PF02991	0.253
LIG_LIR_Gen_1	175	182	PF02991	0.222
LIG_LIR_Gen_1	220	226	PF02991	0.226
LIG_LIR_Gen_1	74	83	PF02991	0.280
LIG_LIR_Nem_3	121	125	PF02991	0.253
LIG_LIR_Nem_3	145	149	PF02991	0.241
LIG_LIR_Nem_3	175	180	PF02991	0.267
LIG_LIR_Nem_3	220	225	PF02991	0.241
LIG_LIR_Nem_3	261	267	PF02991	0.226
LIG_LIR_Nem_3	283	289	PF02991	0.312
LIG_LIR_Nem_3	74	78	PF02991	0.280
LIG_LYPXL_yS_3	117	120	PF13949	0.243
LIG_PDZ_Class_2	300	305	PF00595	0.295
LIG_Pex14_2	206	210	PF04695	0.204
LIG_Pex14_2	217	221	PF04695	0.250
LIG_Pex14_2	278	282	PF04695	0.277
LIG_PTB_Apo_2	216	223	PF02174	0.257
LIG_SH2_GRB2like	271	274	PF00017	0.220
LIG_SH2_PTP2	131	134	PF00017	0.309
LIG_SH2_PTP2	267	270	PF00017	0.280
LIG_SH2_STAP1	122	126	PF00017	0.226
LIG_SH2_STAT5	131	134	PF00017	0.326
LIG_SH2_STAT5	267	270	PF00017	0.308
LIG_SH3_3	137	143	PF00018	0.226
LIG_SH3_3	153	159	PF00018	0.243
LIG_SH3_3	228	234	PF00018	0.280
LIG_SH3_4	92	99	PF00018	0.168
LIG_SUMO_SIM_anti_2	186	192	PF11976	0.241
LIG_TYR_ITIM	120	125	PF00017	0.226
MOD_CK1_1	19	25	PF00069	0.334
MOD_CK1_1	220	226	PF00069	0.228
MOD_CK1_1	254	260	PF00069	0.220
MOD_CK1_1	28	34	PF00069	0.609
MOD_CK1_1	45	51	PF00069	0.279
MOD_CK2_1	192	198	PF00069	0.243
MOD_CK2_1	45	51	PF00069	0.215
MOD_GlcNHglycan	203	206	PF01048	0.444
MOD_GlcNHglycan	21	24	PF01048	0.348
MOD_GlcNHglycan	281	285	PF01048	0.404
MOD_GlcNHglycan	85	88	PF01048	0.443
MOD_GSK3_1	138	145	PF00069	0.168
MOD_GSK3_1	220	227	PF00069	0.371
MOD_GSK3_1	287	294	PF00069	0.337
MOD_NEK2_1	1	6	PF00069	0.683
MOD_NEK2_1	201	206	PF00069	0.189
MOD_NEK2_1	217	222	PF00069	0.269
MOD_NEK2_1	25	30	PF00069	0.450
MOD_PIKK_1	166	172	PF00454	0.238
MOD_PIKK_1	220	226	PF00454	0.306
MOD_PK_1	152	158	PF00069	0.226
MOD_PKA_1	287	293	PF00069	0.351
MOD_PKA_2	243	249	PF00069	0.268
MOD_Plk_1	217	223	PF00069	0.226
MOD_Plk_1	254	260	PF00069	0.289
MOD_Plk_2-3	71	77	PF00069	0.243
MOD_Plk_4	16	22	PF00069	0.303
MOD_Plk_4	217	223	PF00069	0.226
MOD_Plk_4	254	260	PF00069	0.316
MOD_Plk_4	60	66	PF00069	0.252
MOD_SUMO_for_1	91	94	PF00179	0.268
MOD_SUMO_rev_2	38	46	PF00179	0.290
TRG_DiLeu_BaEn_1	186	191	PF01217	0.226
TRG_ENDOCYTIC_2	117	120	PF00928	0.243
TRG_ENDOCYTIC_2	122	125	PF00928	0.226
TRG_ENDOCYTIC_2	131	134	PF00928	0.134
TRG_ENDOCYTIC_2	203	206	PF00928	0.220
TRG_ENDOCYTIC_2	267	270	PF00928	0.250
TRG_ENDOCYTIC_2	75	78	PF00928	0.297
TRG_ER_diArg_1	106	108	PF00400	0.168
TRG_NES_CRM1_1	124	135	PF08389	0.243
TRG_NLS_MonoExtC_3	113	118	PF00514	0.243
TRG_NLS_MonoExtN_4	112	118	PF00514	0.247
TRG_Pf-PMV_PEXEL_1	287	291	PF00026	0.546

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P3Y9	Leptomonas seymouri	27%	75%
A0A0N0P6I0	Leptomonas seymouri	78%	94%
A0A0S4IHN4	Bodo saltans	56%	95%
A0A1X0P9U7	Trypanosomatidae	67%	93%
A0A3S7WWR4	Leishmania donovani	24%	89%
A2XFC7	Oryza sativa subsp. indica	42%	100%
A2YPX3	Oryza sativa subsp. indica	22%	97%
A4HC12	Leishmania braziliensis	27%	78%
A4HZF3	Leishmania infantum	24%	89%
A4I9H5	Leishmania infantum	85%	100%
A4QVH4	Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958)	50%	84%
A4R606	Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958)	45%	100%
A5H454	Zea mays	24%	95%
A7NY33	Vitis vinifera	23%	95%
E9AVE9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	25%	89%
E9B4H3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	84%	100%
H6V7N3	Leishmania donovani	85%	100%
O48677	Arabidopsis thaliana	27%	94%
O80912	Arabidopsis thaliana	24%	87%
O81772	Arabidopsis thaliana	26%	94%
O94753	Pleurotus eryngii	23%	84%
P00431	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	45%	84%
P00434	Brassica rapa subsp. rapa	22%	100%
P0C0L0	Oryza sativa subsp. japonica	37%	95%
P0C0L1	Oryza sativa subsp. japonica	38%	99%
P0C0V3	Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)	51%	84%
P0CP54	Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565)	44%	81%
P0CP55	Cryptococcus neoformans var. neoformans serotype D (strain B-3501A)	44%	81%
P0CP56	Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565)	43%	97%
P0CP57	Cryptococcus neoformans var. neoformans serotype D (strain B-3501A)	43%	97%
P15003	Solanum lycopersicum	25%	84%
P15004	Solanum lycopersicum	24%	84%
P17180	Armoracia rusticana	27%	87%
P19136	Phanerodontia chrysosporium	26%	80%
P27337	Hordeum vulgare	22%	97%
P48534	Pisum sativum	43%	100%
P82281	Arabidopsis thaliana	26%	87%
Q01MI9	Oryza sativa subsp. indica	42%	100%
Q02200	Nicotiana sylvestris	25%	95%
Q02567	Phanerodontia chrysosporium	27%	81%
Q05431	Arabidopsis thaliana	41%	100%
Q05855	Triticum aestivum	25%	98%
Q0D3N0	Oryza sativa subsp. japonica	22%	97%
Q0JEQ2	Oryza sativa subsp. japonica	43%	100%
Q10N21	Oryza sativa subsp. japonica	42%	100%
Q1PER6	Arabidopsis thaliana	40%	100%
Q42564	Arabidopsis thaliana	43%	100%
Q42592	Arabidopsis thaliana	38%	82%
Q42593	Arabidopsis thaliana	37%	72%
Q43729	Arabidopsis thaliana	27%	97%
Q43873	Arabidopsis thaliana	22%	93%
Q4HWQ2	Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1)	44%	92%
Q4ING3	Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1)	48%	85%
Q4PBY6	Ustilago maydis (strain 521 / FGSC 9021)	48%	77%
Q4PD66	Ustilago maydis (strain 521 / FGSC 9021)	46%	92%
Q4Q3K2	Leishmania major	82%	100%
Q4WLG9	Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100)	46%	95%
Q4WPF8	Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100)	51%	83%
Q539E5	Hydra viridissima	27%	83%
Q59X94	Candida albicans (strain SC5314 / ATCC MYA-2876)	50%	100%
Q5AEN1	Candida albicans (strain SC5314 / ATCC MYA-2876)	42%	83%
Q5B1Z0	Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)	46%	98%
Q6BIB1	Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968)	46%	71%
Q6BKY9	Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968)	44%	85%
Q6C0Z6	Yarrowia lipolytica (strain CLIB 122 / E 150)	46%	90%
Q6C7U1	Yarrowia lipolytica (strain CLIB 122 / E 150)	50%	100%
Q6CAB5	Yarrowia lipolytica (strain CLIB 122 / E 150)	50%	100%
Q6CW24	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)	44%	88%
Q6FMG7	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	43%	85%
Q6URB0	Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487)	49%	81%
Q6ZJJ1	Oryza sativa subsp. japonica	43%	100%
Q70LM3	Phlebia radiata	26%	78%
Q7F1U0	Oryza sativa subsp. japonica	23%	96%
Q7SDV9	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)	50%	85%
Q7XJ02	Oryza sativa subsp. japonica	39%	85%
Q7XZP5	Arabidopsis thaliana	42%	100%
Q8GY91	Arabidopsis thaliana	31%	93%
Q96510	Arabidopsis thaliana	24%	93%
Q96518	Arabidopsis thaliana	23%	94%
Q96522	Arabidopsis thaliana	23%	94%
Q96TS6	Phlebia radiata	28%	84%
Q9FE01	Oryza sativa subsp. japonica	42%	100%
Q9FJZ9	Arabidopsis thaliana	25%	91%
Q9FL16	Arabidopsis thaliana	28%	93%
Q9FLC0	Arabidopsis thaliana	21%	94%
Q9FX85	Arabidopsis thaliana	25%	87%
Q9LE15	Arabidopsis thaliana	28%	97%
Q9LHA7	Arabidopsis thaliana	26%	97%
Q9LSP0	Arabidopsis thaliana	27%	90%
Q9SK52	Arabidopsis thaliana	27%	93%
Q9SY33	Arabidopsis thaliana	25%	87%
Q9SZB9	Arabidopsis thaliana	25%	94%
Q9THX6	Solanum lycopersicum	27%	88%
Q9UR19	Pleurotus eryngii	23%	84%
V5BM56	Trypanosoma cruzi	69%	100%