Vacuolar protein-sorting-associated protein 36

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Vacuolar protein sorting protein 36 Vps36/EAP30/Vps36 family, putative

Species:

Leishmania braziliensis

UniProt:

A4HA31_LEIBR

TriTrypDb:

LbrM.19.1050 , LBRM2903_190016600

Length:

466

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 3
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0000814	ESCRT II complex	3	11
GO:0010008	endosome membrane	5	11
GO:0012506	vesicle membrane	4	11
GO:0016020	membrane	2	11
GO:0030659	cytoplasmic vesicle membrane	5	11
GO:0031090	organelle membrane	3	11
GO:0031902	late endosome membrane	6	11
GO:0032991	protein-containing complex	1	11
GO:0036452	ESCRT complex	2	11
GO:0098588	bounding membrane of organelle	4	11
GO:0098796	membrane protein complex	2	11
GO:0110165	cellular anatomical entity	1	11
GO:0005737	cytoplasm	2	1

Expansion

Sequence features

A4HA31

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HA31

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	11
GO:0006886	intracellular protein transport	4	11
GO:0007034	vacuolar transport	4	11
GO:0008104	protein localization	4	11
GO:0009987	cellular process	1	11
GO:0015031	protein transport	4	11
GO:0016192	vesicle-mediated transport	4	11
GO:0016197	endosomal transport	4	11
GO:0032509	endosome transport via multivesicular body sorting pathway	5	11
GO:0032511	late endosome to vacuole transport via multivesicular body sorting pathway	6	11
GO:0033036	macromolecule localization	2	11
GO:0033365	protein localization to organelle	5	11
GO:0043328	protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway	5	11
GO:0045184	establishment of protein localization	3	11
GO:0045324	late endosome to vacuole transport	5	11
GO:0046907	intracellular transport	3	11
GO:0051179	localization	1	11
GO:0051234	establishment of localization	2	11
GO:0051641	cellular localization	2	11
GO:0051649	establishment of localization in cell	3	11
GO:0070727	cellular macromolecule localization	3	11
GO:0071702	organic substance transport	4	11
GO:0071705	nitrogen compound transport	4	11
GO:0071985	multivesicular body sorting pathway	5	11
GO:0072594	establishment of protein localization to organelle	4	11
GO:0072665	protein localization to vacuole	6	11
GO:0072666	establishment of protein localization to vacuole	5	11

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	11
GO:0005515	protein binding	2	11
GO:0005543	phospholipid binding	3	11
GO:0008289	lipid binding	2	11
GO:0032182	ubiquitin-like protein binding	3	11
GO:0032266	phosphatidylinositol-3-phosphate binding	6	11
GO:0035091	phosphatidylinositol binding	4	11
GO:0043130	ubiquitin binding	4	11
GO:1901981	phosphatidylinositol phosphate binding	5	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	220	222	PF00675	0.361
CLV_NRD_NRD_1	346	348	PF00675	0.316
CLV_NRD_NRD_1	37	39	PF00675	0.257
CLV_NRD_NRD_1	69	71	PF00675	0.338
CLV_PCSK_KEX2_1	220	222	PF00082	0.361
CLV_PCSK_KEX2_1	37	39	PF00082	0.272
CLV_PCSK_KEX2_1	69	71	PF00082	0.338
CLV_PCSK_SKI1_1	327	331	PF00082	0.225
CLV_PCSK_SKI1_1	347	351	PF00082	0.273
CLV_PCSK_SKI1_1	401	405	PF00082	0.242
CLV_PCSK_SKI1_1	41	45	PF00082	0.181
CLV_PCSK_SKI1_1	413	417	PF00082	0.300
CLV_PCSK_SKI1_1	444	448	PF00082	0.181
CLV_Separin_Metazoa	66	70	PF03568	0.538
DEG_Nend_UBRbox_2	1	3	PF02207	0.561
DEG_SPOP_SBC_1	164	168	PF00917	0.620
DEG_SPOP_SBC_1	170	174	PF00917	0.572
DOC_CKS1_1	146	151	PF01111	0.494
DOC_CYCLIN_RxL_1	410	420	PF00134	0.381
DOC_CYCLIN_yCln2_LP_2	146	152	PF00134	0.495
DOC_CYCLIN_yCln2_LP_2	316	322	PF00134	0.538
DOC_CYCLIN_yCln2_LP_2	61	67	PF00134	0.561
DOC_MAPK_DCC_7	312	322	PF00069	0.538
DOC_MAPK_gen_1	271	280	PF00069	0.529
DOC_MAPK_gen_1	34	44	PF00069	0.544
DOC_MAPK_gen_1	413	423	PF00069	0.534
DOC_MAPK_MEF2A_6	271	280	PF00069	0.538
DOC_PP1_RVXF_1	411	418	PF00149	0.381
DOC_PP1_RVXF_1	98	104	PF00149	0.485
DOC_PP2B_LxvP_1	61	64	PF13499	0.472
DOC_PP4_FxxP_1	84	87	PF00568	0.468
DOC_USP7_MATH_1	129	133	PF00917	0.502
DOC_USP7_MATH_1	169	173	PF00917	0.695
DOC_USP7_MATH_1	403	407	PF00917	0.500
DOC_USP7_MATH_1	409	413	PF00917	0.489
DOC_USP7_MATH_1	55	59	PF00917	0.381
DOC_WW_Pin1_4	145	150	PF00397	0.612
DOC_WW_Pin1_4	160	165	PF00397	0.748
DOC_WW_Pin1_4	171	176	PF00397	0.643
DOC_WW_Pin1_4	315	320	PF00397	0.489
DOC_WW_Pin1_4	373	378	PF00397	0.503
DOC_WW_Pin1_4	383	388	PF00397	0.463
DOC_WW_Pin1_4	394	399	PF00397	0.394
DOC_WW_Pin1_4	83	88	PF00397	0.493
LIG_14-3-3_CanoR_1	401	409	PF00244	0.534
LIG_BRCT_BRCA1_1	289	293	PF00533	0.561
LIG_BRCT_BRCA1_1	77	81	PF00533	0.561
LIG_CtBP_PxDLS_1	64	68	PF00389	0.561
LIG_eIF4E_1	338	344	PF01652	0.381
LIG_FHA_1	111	117	PF00498	0.434
LIG_FHA_1	165	171	PF00498	0.715
LIG_FHA_1	251	257	PF00498	0.451
LIG_FHA_1	328	334	PF00498	0.437
LIG_FHA_1	361	367	PF00498	0.538
LIG_FHA_1	44	50	PF00498	0.458
LIG_FHA_1	458	464	PF00498	0.510
LIG_FHA_2	199	205	PF00498	0.489
LIG_FHA_2	238	244	PF00498	0.487
LIG_FHA_2	402	408	PF00498	0.561
LIG_FHA_2	450	456	PF00498	0.515
LIG_FHA_2	9	15	PF00498	0.490
LIG_GBD_Chelix_1	276	284	PF00786	0.202
LIG_GBD_Chelix_1	433	441	PF00786	0.272
LIG_LIR_Gen_1	109	119	PF02991	0.457
LIG_LIR_Gen_1	290	301	PF02991	0.561
LIG_LIR_Gen_1	336	344	PF02991	0.537
LIG_LIR_Gen_1	460	466	PF02991	0.447
LIG_LIR_Nem_3	109	115	PF02991	0.462
LIG_LIR_Nem_3	2	6	PF02991	0.326
LIG_LIR_Nem_3	303	308	PF02991	0.458
LIG_LIR_Nem_3	336	341	PF02991	0.476
LIG_LIR_Nem_3	451	457	PF02991	0.459
LIG_LIR_Nem_3	460	464	PF02991	0.399
LIG_LIR_Nem_3	78	84	PF02991	0.536
LIG_NRBOX	339	345	PF00104	0.381
LIG_NRBOX	361	367	PF00104	0.468
LIG_NRBOX	437	443	PF00104	0.381
LIG_PDZ_Class_2	461	466	PF00595	0.392
LIG_Pex14_2	111	115	PF04695	0.299
LIG_RPA_C_Fungi	348	360	PF08784	0.456
LIG_SH2_CRK	338	342	PF00017	0.423
LIG_SH2_NCK_1	338	342	PF00017	0.423
LIG_SH2_PTP2	98	101	PF00017	0.478
LIG_SH2_STAP1	112	116	PF00017	0.517
LIG_SH2_STAP1	459	463	PF00017	0.499
LIG_SH2_STAT5	112	115	PF00017	0.408
LIG_SH2_STAT5	457	460	PF00017	0.419
LIG_SH2_STAT5	98	101	PF00017	0.405
LIG_SH3_3	172	178	PF00018	0.658
LIG_SH3_3	294	300	PF00018	0.311
LIG_SH3_3	88	94	PF00018	0.322
LIG_SH3_CIN85_PxpxPR_1	176	181	PF14604	0.584
LIG_SUMO_SIM_anti_2	318	324	PF11976	0.456
LIG_SUMO_SIM_anti_2	339	345	PF11976	0.420
LIG_SUMO_SIM_anti_2	436	442	PF11976	0.456
LIG_SUMO_SIM_par_1	339	345	PF11976	0.423
LIG_UBA3_1	304	310	PF00899	0.300
LIG_UBA3_1	437	444	PF00899	0.423
MOD_CDK_SPK_2	83	88	PF00069	0.256
MOD_CDK_SPxxK_3	394	401	PF00069	0.203
MOD_CK1_1	145	151	PF00069	0.530
MOD_CK1_1	160	166	PF00069	0.777
MOD_CK1_1	192	198	PF00069	0.423
MOD_CK1_1	249	255	PF00069	0.395
MOD_CK1_1	386	392	PF00069	0.307
MOD_CK1_1	419	425	PF00069	0.421
MOD_CK1_1	76	82	PF00069	0.368
MOD_CK2_1	237	243	PF00069	0.368
MOD_CK2_1	419	425	PF00069	0.380
MOD_CK2_1	8	14	PF00069	0.203
MOD_Cter_Amidation	345	348	PF01082	0.423
MOD_GlcNHglycan	150	153	PF01048	0.707
MOD_GlcNHglycan	159	162	PF01048	0.665
MOD_GlcNHglycan	191	194	PF01048	0.334
MOD_GlcNHglycan	333	336	PF01048	0.363
MOD_GlcNHglycan	344	347	PF01048	0.269
MOD_GlcNHglycan	373	376	PF01048	0.334
MOD_GlcNHglycan	70	73	PF01048	0.319
MOD_GlcNHglycan	78	81	PF01048	0.299
MOD_GSK3_1	144	151	PF00069	0.561
MOD_GSK3_1	156	163	PF00069	0.780
MOD_GSK3_1	165	172	PF00069	0.715
MOD_GSK3_1	210	217	PF00069	0.205
MOD_GSK3_1	231	238	PF00069	0.370
MOD_GSK3_1	242	249	PF00069	0.270
MOD_GSK3_1	258	265	PF00069	0.256
MOD_GSK3_1	327	334	PF00069	0.367
MOD_GSK3_1	382	389	PF00069	0.399
MOD_N-GLC_1	198	203	PF02516	0.325
MOD_N-GLC_1	265	270	PF02516	0.418
MOD_N-GLC_1	353	358	PF02516	0.203
MOD_N-GLC_2	324	326	PF02516	0.400
MOD_NEK2_1	198	203	PF00069	0.354
MOD_NEK2_1	42	47	PF00069	0.331
MOD_PIKK_1	129	135	PF00454	0.527
MOD_PIKK_1	19	25	PF00454	0.213
MOD_PIKK_1	386	392	PF00454	0.291
MOD_PK_1	246	252	PF00069	0.359
MOD_PKA_2	68	74	PF00069	0.456
MOD_Plk_1	198	204	PF00069	0.325
MOD_Plk_1	250	256	PF00069	0.300
MOD_Plk_1	353	359	PF00069	0.384
MOD_Plk_1	419	425	PF00069	0.447
MOD_Plk_1	55	61	PF00069	0.456
MOD_Plk_4	211	217	PF00069	0.201
MOD_Plk_4	24	30	PF00069	0.434
MOD_Plk_4	255	261	PF00069	0.265
MOD_Plk_4	339	345	PF00069	0.362
MOD_Plk_4	353	359	PF00069	0.315
MOD_Plk_4	361	367	PF00069	0.331
MOD_Plk_4	56	62	PF00069	0.355
MOD_ProDKin_1	145	151	PF00069	0.614
MOD_ProDKin_1	160	166	PF00069	0.749
MOD_ProDKin_1	171	177	PF00069	0.639
MOD_ProDKin_1	315	321	PF00069	0.354
MOD_ProDKin_1	373	379	PF00069	0.374
MOD_ProDKin_1	383	389	PF00069	0.318
MOD_ProDKin_1	394	400	PF00069	0.222
MOD_ProDKin_1	83	89	PF00069	0.360
MOD_SUMO_rev_2	117	123	PF00179	0.286
MOD_SUMO_rev_2	412	418	PF00179	0.456
TRG_DiLeu_BaLyEn_6	297	302	PF01217	0.314
TRG_ENDOCYTIC_2	112	115	PF00928	0.436
TRG_ENDOCYTIC_2	3	6	PF00928	0.314
TRG_ENDOCYTIC_2	338	341	PF00928	0.423
TRG_ENDOCYTIC_2	98	101	PF00928	0.408
TRG_ER_diArg_1	219	221	PF00400	0.456
TRG_ER_diArg_1	68	70	PF00400	0.423
TRG_NES_CRM1_1	414	425	PF08389	0.456
TRG_Pf-PMV_PEXEL_1	220	224	PF00026	0.370

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P3P0	Leptomonas seymouri	66%	100%
A0A1X0P698	Trypanosomatidae	43%	100%
A0A3R7M0G6	Trypanosoma rangeli	41%	100%
A0A3S7WVL4	Leishmania donovani	77%	100%
A4HY94	Leishmania infantum	77%	100%
A5PK00	Bos taurus	26%	100%
D0A0M8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	35%	100%
E9AS26	Leishmania mexicana (strain MHOM/GT/2001/U1103)	76%	100%
P0C0A2	Rattus norvegicus	27%	100%
Q4QDE7	Leishmania major	75%	96%
Q6DDF4	Xenopus laevis	26%	100%
Q7ZVK4	Danio rerio	26%	100%
Q86VN1	Homo sapiens	27%	100%
Q91XD6	Mus musculus	27%	100%
Q9VU87	Drosophila melanogaster	24%	100%
V5AMS4	Trypanosoma cruzi	42%	100%