Plastid-encoded RNA polymerase subunit alpha

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

DNA-directed RNA polymerases I and III subunit RPAC1, putative

Species:

Leishmania braziliensis

UniProt:

A4HA24_LEIBR

TriTrypDb:

LbrM.19.0980 , LBRM2903_190015500

Length:

446

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0000428	DNA-directed RNA polymerase complex	4	12
GO:0030880	RNA polymerase complex	3	12
GO:0032991	protein-containing complex	1	12
GO:0061695	transferase complex, transferring phosphorus-containing groups	4	12
GO:0140535	intracellular protein-containing complex	2	12
GO:1902494	catalytic complex	2	12
GO:1990234	transferase complex	3	12
GO:0005634	nucleus	5	1
GO:0005666	RNA polymerase III complex	4	1
GO:0005730	nucleolus	5	1
GO:0005736	RNA polymerase I complex	4	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1
GO:0055029	nuclear DNA-directed RNA polymerase complex	3	1
GO:0110165	cellular anatomical entity	1	1
GO:0140513	nuclear protein-containing complex	2	1

Expansion

Sequence features

A4HA24

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HA24

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006351	DNA-templated transcription	7	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0009058	biosynthetic process	2	1
GO:0009059	macromolecule biosynthetic process	4	1
GO:0009987	cellular process	1	1
GO:0016070	RNA metabolic process	5	1
GO:0018130	heterocycle biosynthetic process	4	1
GO:0019438	aromatic compound biosynthetic process	4	1
GO:0032774	RNA biosynthetic process	5	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0034654	nucleobase-containing compound biosynthetic process	4	1
GO:0043170	macromolecule metabolic process	3	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044249	cellular biosynthetic process	3	1
GO:0044271	cellular nitrogen compound biosynthetic process	4	1
GO:0046483	heterocycle metabolic process	3	1
GO:0071704	organic substance metabolic process	2	1
GO:0090304	nucleic acid metabolic process	4	1
GO:0097659	nucleic acid-templated transcription	6	1
GO:1901360	organic cyclic compound metabolic process	3	1
GO:1901362	organic cyclic compound biosynthetic process	4	1
GO:1901576	organic substance biosynthetic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0001054	RNA polymerase I activity	7	12
GO:0001056	RNA polymerase III activity	7	12
GO:0003824	catalytic activity	1	12
GO:0003899	DNA-directed 5'-3' RNA polymerase activity	6	12
GO:0005488	binding	1	12
GO:0005515	protein binding	2	12
GO:0016740	transferase activity	2	12
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	12
GO:0016779	nucleotidyltransferase activity	4	12
GO:0034062	5'-3' RNA polymerase activity	5	12
GO:0046983	protein dimerization activity	3	12
GO:0097747	RNA polymerase activity	4	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	108	112	PF00656	0.634
CLV_C14_Caspase3-7	157	161	PF00656	0.428
CLV_NRD_NRD_1	118	120	PF00675	0.472
CLV_NRD_NRD_1	139	141	PF00675	0.228
CLV_NRD_NRD_1	5	7	PF00675	0.717
CLV_PCSK_KEX2_1	118	120	PF00082	0.468
CLV_PCSK_KEX2_1	139	141	PF00082	0.228
CLV_PCSK_KEX2_1	5	7	PF00082	0.717
CLV_PCSK_KEX2_1	73	75	PF00082	0.698
CLV_PCSK_PC1ET2_1	73	75	PF00082	0.729
CLV_PCSK_SKI1_1	139	143	PF00082	0.255
CLV_PCSK_SKI1_1	202	206	PF00082	0.257
DEG_APCC_DBOX_1	170	178	PF00400	0.439
DEG_APCC_DBOX_1	201	209	PF00400	0.471
DEG_APCC_DBOX_1	287	295	PF00400	0.428
DEG_SCF_FBW7_1	218	223	PF00400	0.496
DEG_SPOP_SBC_1	92	96	PF00917	0.622
DOC_ANK_TNKS_1	106	113	PF00023	0.635
DOC_CDC14_PxL_1	143	151	PF14671	0.385
DOC_CYCLIN_RxL_1	199	209	PF00134	0.439
DOC_MAPK_gen_1	118	125	PF00069	0.412
DOC_MAPK_gen_1	288	296	PF00069	0.428
DOC_MAPK_gen_1	3	13	PF00069	0.695
DOC_MAPK_gen_1	387	397	PF00069	0.448
DOC_PIKK_1	159	167	PF02985	0.524
DOC_PP1_RVXF_1	200	207	PF00149	0.428
DOC_USP7_MATH_1	31	35	PF00917	0.712
DOC_USP7_MATH_1	378	382	PF00917	0.432
DOC_USP7_MATH_1	47	51	PF00917	0.639
DOC_USP7_MATH_1	68	72	PF00917	0.723
DOC_USP7_MATH_1	92	96	PF00917	0.729
DOC_USP7_UBL2_3	237	241	PF12436	0.364
DOC_USP7_UBL2_3	69	73	PF12436	0.702
DOC_WW_Pin1_4	128	133	PF00397	0.464
DOC_WW_Pin1_4	158	163	PF00397	0.528
DOC_WW_Pin1_4	216	221	PF00397	0.481
DOC_WW_Pin1_4	23	28	PF00397	0.641
DOC_WW_Pin1_4	88	93	PF00397	0.701
LIG_14-3-3_CanoR_1	107	111	PF00244	0.605
LIG_14-3-3_CanoR_1	122	126	PF00244	0.287
LIG_14-3-3_CanoR_1	140	149	PF00244	0.342
LIG_14-3-3_CanoR_1	53	62	PF00244	0.579
LIG_Actin_WH2_2	276	294	PF00022	0.428
LIG_Actin_WH2_2	398	414	PF00022	0.512
LIG_APCC_ABBA_1	272	277	PF00400	0.428
LIG_APCC_ABBA_1	415	420	PF00400	0.439
LIG_BIR_II_1	1	5	PF00653	0.633
LIG_BIR_III_4	209	213	PF00653	0.524
LIG_BRCT_BRCA1_1	134	138	PF00533	0.455
LIG_Clathr_ClatBox_1	205	209	PF01394	0.493
LIG_EVH1_2	62	66	PF00568	0.650
LIG_FHA_1	115	121	PF00498	0.465
LIG_FHA_1	19	25	PF00498	0.711
LIG_FHA_1	391	397	PF00498	0.481
LIG_FHA_2	106	112	PF00498	0.590
LIG_FHA_2	194	200	PF00498	0.459
LIG_FHA_2	262	268	PF00498	0.374
LIG_FHA_2	316	322	PF00498	0.527
LIG_FHA_2	84	90	PF00498	0.522
LIG_LIR_Gen_1	148	156	PF02991	0.493
LIG_LIR_Gen_1	194	203	PF02991	0.462
LIG_LIR_Nem_3	135	141	PF02991	0.532
LIG_LIR_Nem_3	148	154	PF02991	0.458
LIG_LIR_Nem_3	194	198	PF02991	0.462
LIG_NRBOX	163	169	PF00104	0.524
LIG_SH2_NCK_1	100	104	PF00017	0.438
LIG_SH2_SRC	345	348	PF00017	0.364
LIG_SH2_STAP1	222	226	PF00017	0.521
LIG_SH2_STAT3	222	225	PF00017	0.524
LIG_SH2_STAT5	186	189	PF00017	0.453
LIG_SH3_3	127	133	PF00018	0.493
LIG_SH3_3	174	180	PF00018	0.458
LIG_SH3_3	21	27	PF00018	0.673
LIG_SH3_3	226	232	PF00018	0.516
LIG_SH3_3	353	359	PF00018	0.493
LIG_SH3_3	41	47	PF00018	0.480
LIG_TRAF2_1	232	235	PF00917	0.357
MOD_CK1_1	26	32	PF00069	0.622
MOD_CK1_1	34	40	PF00069	0.615
MOD_CK1_1	91	97	PF00069	0.635
MOD_CK2_1	315	321	PF00069	0.530
MOD_CK2_1	326	332	PF00069	0.527
MOD_CK2_1	83	89	PF00069	0.703
MOD_Cter_Amidation	71	74	PF01082	0.507
MOD_GlcNHglycan	103	106	PF01048	0.490
MOD_GlcNHglycan	309	312	PF01048	0.253
MOD_GlcNHglycan	328	331	PF01048	0.162
MOD_GlcNHglycan	438	442	PF01048	0.626
MOD_GlcNHglycan	55	58	PF01048	0.506
MOD_GlcNHglycan	63	66	PF01048	0.549
MOD_GlcNHglycan	70	73	PF01048	0.626
MOD_GSK3_1	101	108	PF00069	0.576
MOD_GSK3_1	128	135	PF00069	0.535
MOD_GSK3_1	141	148	PF00069	0.458
MOD_GSK3_1	18	25	PF00069	0.608
MOD_GSK3_1	187	194	PF00069	0.478
MOD_GSK3_1	216	223	PF00069	0.433
MOD_GSK3_1	34	41	PF00069	0.616
MOD_GSK3_1	88	95	PF00069	0.649
MOD_N-GLC_1	158	163	PF02516	0.253
MOD_N-GLC_1	32	37	PF02516	0.482
MOD_NEK2_1	1	6	PF00069	0.601
MOD_NEK2_1	326	331	PF00069	0.491
MOD_NEK2_1	334	339	PF00069	0.489
MOD_PKA_2	106	112	PF00069	0.640
MOD_PKA_2	121	127	PF00069	0.363
MOD_PKA_2	324	330	PF00069	0.532
MOD_Plk_1	390	396	PF00069	0.453
MOD_Plk_2-3	121	127	PF00069	0.307
MOD_Plk_2-3	261	267	PF00069	0.364
MOD_Plk_4	352	358	PF00069	0.512
MOD_Plk_4	94	100	PF00069	0.674
MOD_ProDKin_1	128	134	PF00069	0.464
MOD_ProDKin_1	158	164	PF00069	0.528
MOD_ProDKin_1	216	222	PF00069	0.481
MOD_ProDKin_1	23	29	PF00069	0.641
MOD_ProDKin_1	88	94	PF00069	0.698
MOD_SUMO_rev_2	310	319	PF00179	0.439
TRG_ENDOCYTIC_2	313	316	PF00928	0.524
TRG_ER_diArg_1	118	120	PF00400	0.473
TRG_ER_diArg_1	138	140	PF00400	0.319
TRG_ER_diArg_1	385	388	PF00400	0.506
TRG_Pf-PMV_PEXEL_1	140	145	PF00026	0.283
TRG_Pf-PMV_PEXEL_1	153	157	PF00026	0.187
TRG_Pf-PMV_PEXEL_1	202	207	PF00026	0.239
TRG_Pf-PMV_PEXEL_1	423	427	PF00026	0.505

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P8L4	Leptomonas seymouri	71%	100%
A0A0S4IYI3	Bodo saltans	44%	100%
A0A1X0NZD0	Trypanosomatidae	27%	100%
A0A1X0P6H8	Trypanosomatidae	51%	100%
A0A3R7MJ28	Trypanosoma rangeli	48%	100%
A0A3S5H758	Leishmania donovani	84%	100%
A0A3S7X218	Leishmania donovani	26%	100%
A0A422NUV2	Trypanosoma rangeli	27%	100%
A4HH22	Leishmania braziliensis	25%	100%
A4HY87	Leishmania infantum	84%	100%
A4I451	Leishmania infantum	26%	100%
D0A0L6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	44%	100%
E9ADL6	Leishmania major	26%	100%
E9AM65	Leishmania mexicana (strain MHOM/GT/2001/U1103)	26%	100%
E9AS18	Leishmania mexicana (strain MHOM/GT/2001/U1103)	82%	100%
O15160	Homo sapiens	29%	100%
O94616	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	31%	100%
P07703	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	27%	100%
P52432	Mus musculus	30%	100%
Q32L22	Bos taurus	31%	100%
Q39211	Arabidopsis thaliana	26%	100%
Q4QDF5	Leishmania major	83%	99%
Q54DH7	Dictyostelium discoideum	27%	100%
Q54SN4	Dictyostelium discoideum	29%	100%
V5BBX7	Trypanosoma cruzi	48%	100%
V5BUI6	Trypanosoma cruzi	26%	100%