Glutamate--cysteine ligase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

gamma-glutamylcysteine synthetase, putative

Species:

Leishmania braziliensis

UniProt:

A4H9R8_LEIBR

TriTrypDb:

LbrM.18.1700 , LBRM2903_180023100

Length:

688

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005654	nucleoplasm	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4H9R8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H9R8

Function

Biological processes
Term	Name	Level	Count
GO:0006518	peptide metabolic process	4	12
GO:0006575	cellular modified amino acid metabolic process	3	12
GO:0006749	glutathione metabolic process	4	12
GO:0006750	glutathione biosynthetic process	5	12
GO:0006790	sulfur compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009058	biosynthetic process	2	12
GO:0009987	cellular process	1	12
GO:0019184	nonribosomal peptide biosynthetic process	6	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0042398	cellular modified amino acid biosynthetic process	4	12
GO:0043043	peptide biosynthetic process	5	12
GO:0043603	amide metabolic process	3	12
GO:0043604	amide biosynthetic process	4	12
GO:0044237	cellular metabolic process	2	12
GO:0044249	cellular biosynthetic process	3	12
GO:0044271	cellular nitrogen compound biosynthetic process	4	12
GO:0044272	sulfur compound biosynthetic process	4	12
GO:0071704	organic substance metabolic process	2	12
GO:1901564	organonitrogen compound metabolic process	3	12
GO:1901566	organonitrogen compound biosynthetic process	4	12
GO:1901576	organic substance biosynthetic process	3	12

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	12
GO:0003824	catalytic activity	1	12
GO:0004357	glutamate-cysteine ligase activity	5	12
GO:0005488	binding	1	12
GO:0005524	ATP binding	5	12
GO:0016874	ligase activity	2	12
GO:0016879	ligase activity, forming carbon-nitrogen bonds	3	12
GO:0016881	acid-amino acid ligase activity	4	12
GO:0017076	purine nucleotide binding	4	12
GO:0030554	adenyl nucleotide binding	5	12
GO:0032553	ribonucleotide binding	3	12
GO:0032555	purine ribonucleotide binding	4	12
GO:0032559	adenyl ribonucleotide binding	5	12
GO:0035639	purine ribonucleoside triphosphate binding	4	12
GO:0036094	small molecule binding	2	12
GO:0043167	ion binding	2	12
GO:0043168	anion binding	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:0097367	carbohydrate derivative binding	2	12
GO:1901265	nucleoside phosphate binding	3	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	115	119	PF00656	0.390
CLV_C14_Caspase3-7	414	418	PF00656	0.373
CLV_NRD_NRD_1	189	191	PF00675	0.294
CLV_NRD_NRD_1	385	387	PF00675	0.253
CLV_NRD_NRD_1	557	559	PF00675	0.257
CLV_NRD_NRD_1	632	634	PF00675	0.330
CLV_PCSK_FUR_1	187	191	PF00082	0.290
CLV_PCSK_KEX2_1	189	191	PF00082	0.294
CLV_PCSK_KEX2_1	385	387	PF00082	0.295
CLV_PCSK_KEX2_1	605	607	PF00082	0.293
CLV_PCSK_KEX2_1	643	645	PF00082	0.341
CLV_PCSK_PC1ET2_1	605	607	PF00082	0.457
CLV_PCSK_PC1ET2_1	643	645	PF00082	0.375
CLV_PCSK_SKI1_1	272	276	PF00082	0.513
CLV_PCSK_SKI1_1	365	369	PF00082	0.234
CLV_PCSK_SKI1_1	374	378	PF00082	0.234
CLV_PCSK_SKI1_1	559	563	PF00082	0.268
CLV_PCSK_SKI1_1	634	638	PF00082	0.328
DEG_COP1_1	162	170	PF00400	0.313
DEG_SPOP_SBC_1	243	247	PF00917	0.501
DOC_CKS1_1	274	279	PF01111	0.372
DOC_CKS1_1	504	509	PF01111	0.234
DOC_CYCLIN_RxL_1	269	279	PF00134	0.533
DOC_CYCLIN_RxL_1	362	372	PF00134	0.328
DOC_CYCLIN_yCln2_LP_2	274	280	PF00134	0.417
DOC_MAPK_DCC_7	192	202	PF00069	0.363
DOC_MAPK_gen_1	187	196	PF00069	0.295
DOC_MAPK_gen_1	395	403	PF00069	0.268
DOC_MAPK_gen_1	40	48	PF00069	0.461
DOC_MAPK_gen_1	438	448	PF00069	0.168
DOC_MAPK_GRA24_9	186	202	PF00069	0.364
DOC_MAPK_HePTP_8	186	198	PF00069	0.295
DOC_MAPK_MEF2A_6	189	198	PF00069	0.299
DOC_MAPK_MEF2A_6	397	405	PF00069	0.268
DOC_PP1_RVXF_1	493	499	PF00149	0.234
DOC_PP1_RVXF_1	526	532	PF00149	0.157
DOC_PP2B_LxvP_1	165	168	PF13499	0.317
DOC_PP4_FxxP_1	483	486	PF00568	0.328
DOC_USP7_MATH_1	23	27	PF00917	0.286
DOC_USP7_MATH_1	243	247	PF00917	0.705
DOC_USP7_MATH_1	258	262	PF00917	0.465
DOC_USP7_MATH_1	307	311	PF00917	0.327
DOC_USP7_MATH_1	356	360	PF00917	0.234
DOC_USP7_MATH_1	487	491	PF00917	0.298
DOC_WW_Pin1_4	156	161	PF00397	0.343
DOC_WW_Pin1_4	217	222	PF00397	0.516
DOC_WW_Pin1_4	244	249	PF00397	0.462
DOC_WW_Pin1_4	273	278	PF00397	0.365
DOC_WW_Pin1_4	358	363	PF00397	0.234
DOC_WW_Pin1_4	503	508	PF00397	0.234
DOC_WW_Pin1_4	604	609	PF00397	0.379
DOC_WW_Pin1_4	98	103	PF00397	0.438
LIG_14-3-3_CanoR_1	235	242	PF00244	0.581
LIG_14-3-3_CanoR_1	298	304	PF00244	0.358
LIG_14-3-3_CanoR_1	615	620	PF00244	0.225
LIG_14-3-3_CanoR_1	633	642	PF00244	0.332
LIG_Actin_WH2_2	175	191	PF00022	0.308
LIG_APCC_ABBA_1	198	203	PF00400	0.309
LIG_BRCT_BRCA1_1	479	483	PF00533	0.157
LIG_deltaCOP1_diTrp_1	491	498	PF00928	0.293
LIG_eIF4E_1	590	596	PF01652	0.249
LIG_FHA_1	136	142	PF00498	0.292
LIG_FHA_1	181	187	PF00498	0.281
LIG_FHA_1	329	335	PF00498	0.249
LIG_FHA_1	4	10	PF00498	0.389
LIG_FHA_1	519	525	PF00498	0.268
LIG_FHA_1	63	69	PF00498	0.453
LIG_FHA_1	663	669	PF00498	0.342
LIG_FHA_2	207	213	PF00498	0.442
LIG_FHA_2	463	469	PF00498	0.249
LIG_FHA_2	504	510	PF00498	0.234
LIG_FHA_2	565	571	PF00498	0.293
LIG_FHA_2	575	581	PF00498	0.204
LIG_FHA_2	636	642	PF00498	0.420
LIG_LIR_Apic_2	302	307	PF02991	0.340
LIG_LIR_Apic_2	480	486	PF02991	0.379
LIG_LIR_Gen_1	121	132	PF02991	0.417
LIG_LIR_Gen_1	138	146	PF02991	0.474
LIG_LIR_Gen_1	372	382	PF02991	0.234
LIG_LIR_Gen_1	396	405	PF02991	0.243
LIG_LIR_Gen_1	425	433	PF02991	0.297
LIG_LIR_Gen_1	475	486	PF02991	0.234
LIG_LIR_Gen_1	612	621	PF02991	0.238
LIG_LIR_Nem_3	121	127	PF02991	0.413
LIG_LIR_Nem_3	138	143	PF02991	0.480
LIG_LIR_Nem_3	372	378	PF02991	0.234
LIG_LIR_Nem_3	396	401	PF02991	0.243
LIG_LIR_Nem_3	425	429	PF02991	0.273
LIG_LIR_Nem_3	468	473	PF02991	0.234
LIG_LIR_Nem_3	475	481	PF02991	0.234
LIG_LIR_Nem_3	586	592	PF02991	0.248
LIG_LIR_Nem_3	612	616	PF02991	0.238
LIG_LIR_Nem_3	92	96	PF02991	0.313
LIG_PDZ_Class_1	683	688	PF00595	0.434
LIG_Pex14_1	494	498	PF04695	0.234
LIG_Pex14_1	89	93	PF04695	0.316
LIG_Pex14_2	33	37	PF04695	0.405
LIG_PTB_Apo_2	507	514	PF02174	0.237
LIG_PTB_Apo_2	87	94	PF02174	0.349
LIG_PTB_Phospho_1	87	93	PF10480	0.351
LIG_SH2_CRK	124	128	PF00017	0.366
LIG_SH2_CRK	304	308	PF00017	0.333
LIG_SH2_CRK	344	348	PF00017	0.234
LIG_SH2_CRK	398	402	PF00017	0.268
LIG_SH2_CRK	532	536	PF00017	0.286
LIG_SH2_CRK	590	594	PF00017	0.249
LIG_SH2_NCK_1	398	402	PF00017	0.281
LIG_SH2_NCK_1	532	536	PF00017	0.299
LIG_SH2_SRC	599	602	PF00017	0.178
LIG_SH2_STAP1	161	165	PF00017	0.369
LIG_SH2_STAP1	283	287	PF00017	0.369
LIG_SH2_STAP1	344	348	PF00017	0.293
LIG_SH2_STAP1	455	459	PF00017	0.268
LIG_SH2_STAT3	282	285	PF00017	0.345
LIG_SH2_STAT5	201	204	PF00017	0.305
LIG_SH2_STAT5	227	230	PF00017	0.550
LIG_SH2_STAT5	286	289	PF00017	0.459
LIG_SH2_STAT5	342	345	PF00017	0.157
LIG_SH2_STAT5	426	429	PF00017	0.355
LIG_SH2_STAT5	532	535	PF00017	0.234
LIG_SH2_STAT5	555	558	PF00017	0.234
LIG_SH2_STAT5	599	602	PF00017	0.364
LIG_SH2_STAT5	613	616	PF00017	0.123
LIG_SH2_STAT5	673	676	PF00017	0.394
LIG_SH3_3	138	144	PF00018	0.278
LIG_SH3_3	306	312	PF00018	0.382
LIG_SH3_3	512	518	PF00018	0.328
LIG_SH3_3	99	105	PF00018	0.270
LIG_SUMO_SIM_anti_2	345	351	PF11976	0.328
LIG_SUMO_SIM_anti_2	533	540	PF11976	0.328
LIG_SUMO_SIM_par_1	456	463	PF11976	0.245
LIG_SUMO_SIM_par_1	63	69	PF11976	0.457
LIG_TRAF2_1	260	263	PF00917	0.502
LIG_TRFH_1	590	594	PF08558	0.249
LIG_WRC_WIRS_1	676	681	PF05994	0.269
LIG_WRC_WIRS_1	93	98	PF05994	0.310
MOD_CDK_SPxxK_3	358	365	PF00069	0.234
MOD_CK1_1	109	115	PF00069	0.337
MOD_CK1_1	159	165	PF00069	0.324
MOD_CK1_1	646	652	PF00069	0.256
MOD_CK2_1	36	42	PF00069	0.214
MOD_CK2_1	422	428	PF00069	0.305
MOD_CK2_1	503	509	PF00069	0.234
MOD_CK2_1	564	570	PF00069	0.302
MOD_CK2_1	574	580	PF00069	0.207
MOD_CK2_1	674	680	PF00069	0.293
MOD_Cter_Amidation	190	193	PF01082	0.246
MOD_GlcNHglycan	25	28	PF01048	0.272
MOD_GlcNHglycan	325	328	PF01048	0.299
MOD_GlcNHglycan	358	361	PF01048	0.293
MOD_GlcNHglycan	621	624	PF01048	0.342
MOD_GlcNHglycan	648	651	PF01048	0.389
MOD_GSK3_1	112	119	PF00069	0.317
MOD_GSK3_1	135	142	PF00069	0.291
MOD_GSK3_1	155	162	PF00069	0.207
MOD_GSK3_1	203	210	PF00069	0.373
MOD_GSK3_1	254	261	PF00069	0.481
MOD_GSK3_1	393	400	PF00069	0.364
MOD_GSK3_1	473	480	PF00069	0.252
MOD_GSK3_1	560	567	PF00069	0.203
MOD_GSK3_1	570	577	PF00069	0.280
MOD_GSK3_1	615	622	PF00069	0.292
MOD_GSK3_1	62	69	PF00069	0.399
MOD_N-GLC_1	106	111	PF02516	0.277
MOD_N-GLC_1	172	177	PF02516	0.323
MOD_N-GLC_1	180	185	PF02516	0.270
MOD_N-GLC_1	235	240	PF02516	0.670
MOD_N-GLC_1	299	304	PF02516	0.553
MOD_N-GLC_1	422	427	PF02516	0.258
MOD_N-GLC_2	564	566	PF02516	0.178
MOD_NEK2_1	3	8	PF00069	0.397
MOD_NEK2_1	323	328	PF00069	0.249
MOD_NEK2_1	355	360	PF00069	0.234
MOD_NEK2_1	36	41	PF00069	0.210
MOD_NEK2_1	619	624	PF00069	0.276
MOD_NEK2_1	635	640	PF00069	0.222
MOD_NEK2_1	97	102	PF00069	0.295
MOD_NEK2_2	161	166	PF00069	0.344
MOD_NEK2_2	267	272	PF00069	0.387
MOD_NEK2_2	299	304	PF00069	0.359
MOD_NMyristoyl	1	7	PF02799	0.415
MOD_PIKK_1	159	165	PF00454	0.379
MOD_PK_1	305	311	PF00069	0.315
MOD_PK_1	397	403	PF00069	0.268
MOD_PKA_1	643	649	PF00069	0.157
MOD_PKA_2	619	625	PF00069	0.293
MOD_PKA_2	643	649	PF00069	0.271
MOD_Plk_1	106	112	PF00069	0.271
MOD_Plk_1	180	186	PF00069	0.206
MOD_Plk_1	207	213	PF00069	0.372
MOD_Plk_1	299	305	PF00069	0.546
MOD_Plk_1	422	428	PF00069	0.254
MOD_Plk_1	462	468	PF00069	0.263
MOD_Plk_1	574	580	PF00069	0.183
MOD_Plk_1	62	68	PF00069	0.381
MOD_Plk_2-3	203	209	PF00069	0.389
MOD_Plk_4	136	142	PF00069	0.301
MOD_Plk_4	161	167	PF00069	0.340
MOD_Plk_4	299	305	PF00069	0.576
MOD_Plk_4	609	615	PF00069	0.383
MOD_Plk_4	92	98	PF00069	0.312
MOD_ProDKin_1	156	162	PF00069	0.335
MOD_ProDKin_1	217	223	PF00069	0.509
MOD_ProDKin_1	244	250	PF00069	0.463
MOD_ProDKin_1	273	279	PF00069	0.361
MOD_ProDKin_1	358	364	PF00069	0.234
MOD_ProDKin_1	503	509	PF00069	0.234
MOD_ProDKin_1	604	610	PF00069	0.379
MOD_ProDKin_1	98	104	PF00069	0.448
MOD_SUMO_rev_2	601	607	PF00179	0.288
TRG_DiLeu_BaEn_2	91	97	PF01217	0.315
TRG_DiLeu_BaLyEn_6	515	520	PF01217	0.328
TRG_ENDOCYTIC_2	124	127	PF00928	0.315
TRG_ENDOCYTIC_2	344	347	PF00928	0.234
TRG_ENDOCYTIC_2	398	401	PF00928	0.242
TRG_ENDOCYTIC_2	426	429	PF00928	0.269
TRG_ENDOCYTIC_2	455	458	PF00928	0.268
TRG_ENDOCYTIC_2	532	535	PF00928	0.283
TRG_ENDOCYTIC_2	590	593	PF00928	0.248
TRG_ENDOCYTIC_2	613	616	PF00928	0.234
TRG_ENDOCYTIC_2	673	676	PF00928	0.523
TRG_ENDOCYTIC_2	93	96	PF00928	0.310
TRG_ER_diArg_1	186	189	PF00400	0.275
TRG_ER_diArg_1	385	387	PF00400	0.371
TRG_Pf-PMV_PEXEL_1	385	389	PF00026	0.145
TRG_Pf-PMV_PEXEL_1	598	603	PF00026	0.293
TRG_Pf-PMV_PEXEL_1	654	658	PF00026	0.299

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1P9N5	Leptomonas seymouri	71%	100%
A0A0S4IM75	Bodo saltans	53%	100%
A0A1X0P757	Trypanosomatidae	58%	100%
A0A3S5IRL4	Trypanosoma rangeli	59%	100%
A0A3S7WV51	Leishmania donovani	77%	100%
A4HY37	Leishmania infantum	77%	100%
D0A548	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	59%	100%
E9ARV1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	76%	100%
P32477	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	32%	100%
Q09768	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	35%	100%
Q4QDM2	Leishmania major	77%	100%
V5BDE4	Trypanosoma cruzi	59%	100%