Nonspecific nucleoside hydrolasewith

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

nonspecific nucleoside hydrolase

Species:

Leishmania braziliensis

UniProt:

A4H9Q9_LEIBR

TriTrypDb:

LbrM.18.1610 , LBRM2903_180022200

Length:

313

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 2
Forrest at al. (procyclic)	no	yes: 2
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 15

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 5
NetGPI	no	yes: 0, no: 5

Cellular components
Term	Name	Level	Count
GO:0005829	cytosol	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4H9Q9

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H9Q9

PDB structure(s): 5tsq_A

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	6
GO:0006725	cellular aromatic compound metabolic process	3	6
GO:0006807	nitrogen compound metabolic process	2	6
GO:0008152	metabolic process	1	6
GO:0009987	cellular process	1	6
GO:0034641	cellular nitrogen compound metabolic process	3	6
GO:0044237	cellular metabolic process	2	6
GO:0044238	primary metabolic process	2	6
GO:0046483	heterocycle metabolic process	3	6
GO:0071704	organic substance metabolic process	2	6
GO:1901360	organic cyclic compound metabolic process	3	6
GO:1901564	organonitrogen compound metabolic process	3	6
GO:0006152	purine nucleoside catabolic process	6	1
GO:0009056	catabolic process	2	1
GO:0009058	biosynthetic process	2	1
GO:0009116	nucleoside metabolic process	4	1
GO:0009164	nucleoside catabolic process	5	1
GO:0018130	heterocycle biosynthetic process	4	1
GO:0019438	aromatic compound biosynthetic process	4	1
GO:0019439	aromatic compound catabolic process	4	1
GO:0034654	nucleobase-containing compound biosynthetic process	4	1
GO:0034655	nucleobase-containing compound catabolic process	4	1
GO:0034656	nucleobase-containing small molecule catabolic process	4	1
GO:0042278	purine nucleoside metabolic process	5	1
GO:0043094	cellular metabolic compound salvage	3	1
GO:0043101	purine-containing compound salvage	4	1
GO:0044248	cellular catabolic process	3	1
GO:0044249	cellular biosynthetic process	3	1
GO:0044270	cellular nitrogen compound catabolic process	4	1
GO:0044271	cellular nitrogen compound biosynthetic process	4	1
GO:0044281	small molecule metabolic process	2	1
GO:0044282	small molecule catabolic process	3	1
GO:0046700	heterocycle catabolic process	4	1
GO:0055086	nucleobase-containing small molecule metabolic process	3	1
GO:0072521	purine-containing compound metabolic process	4	1
GO:0072522	purine-containing compound biosynthetic process	5	1
GO:0072523	purine-containing compound catabolic process	5	1
GO:1901135	carbohydrate derivative metabolic process	3	1
GO:1901136	carbohydrate derivative catabolic process	4	1
GO:1901361	organic cyclic compound catabolic process	4	1
GO:1901362	organic cyclic compound biosynthetic process	4	1
GO:1901565	organonitrogen compound catabolic process	4	1
GO:1901566	organonitrogen compound biosynthetic process	4	1
GO:1901575	organic substance catabolic process	3	1
GO:1901576	organic substance biosynthetic process	3	1
GO:1901657	glycosyl compound metabolic process	4	1
GO:1901658	glycosyl compound catabolic process	5	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	6
GO:0005488	binding	1	2
GO:0016787	hydrolase activity	2	6
GO:0016798	hydrolase activity, acting on glycosyl bonds	3	6
GO:0016799	hydrolase activity, hydrolyzing N-glycosyl compounds	4	6
GO:0043167	ion binding	2	2
GO:0043169	cation binding	3	2
GO:0045437	uridine nucleosidase activity	6	6
GO:0046872	metal ion binding	4	2
GO:0050263	ribosylpyrimidine nucleosidase activity	5	6
GO:0008477	purine nucleosidase activity	5	3
GO:0047724	inosine nucleosidase activity	6	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	2	4	PF00675	0.588
CLV_NRD_NRD_1	72	74	PF00675	0.279
CLV_PCSK_KEX2_1	102	104	PF00082	0.279
CLV_PCSK_PC1ET2_1	102	104	PF00082	0.279
CLV_PCSK_SKI1_1	119	123	PF00082	0.460
CLV_PCSK_SKI1_1	4	8	PF00082	0.554
DEG_MDM2_SWIB_1	296	304	PF02201	0.660
DOC_CKS1_1	198	203	PF01111	0.660
DOC_CYCLIN_RxL_1	217	226	PF00134	0.479
DOC_MAPK_FxFP_2	278	281	PF00069	0.660
DOC_MAPK_gen_1	99	108	PF00069	0.479
DOC_MAPK_HePTP_8	100	112	PF00069	0.660
DOC_MAPK_MEF2A_6	102	110	PF00069	0.651
DOC_MAPK_MEF2A_6	258	265	PF00069	0.576
DOC_PP1_RVXF_1	218	225	PF00149	0.479
DOC_PP1_RVXF_1	291	297	PF00149	0.660
DOC_PP4_FxxP_1	170	173	PF00568	0.632
DOC_PP4_FxxP_1	278	281	PF00568	0.660
DOC_WW_Pin1_4	159	164	PF00397	0.660
DOC_WW_Pin1_4	197	202	PF00397	0.660
LIG_14-3-3_CanoR_1	233	237	PF00244	0.527
LIG_14-3-3_CanoR_1	73	79	PF00244	0.479
LIG_APCC_ABBA_1	167	172	PF00400	0.632
LIG_EH1_1	103	111	PF00400	0.660
LIG_FHA_1	124	130	PF00498	0.551
LIG_FHA_1	198	204	PF00498	0.660
LIG_FHA_1	254	260	PF00498	0.660
LIG_FHA_1	32	38	PF00498	0.660
LIG_FHA_2	272	278	PF00498	0.563
LIG_FHA_2	96	102	PF00498	0.660
LIG_LIR_Apic_2	276	281	PF02991	0.652
LIG_LIR_Gen_1	166	175	PF02991	0.660
LIG_LIR_Gen_1	223	229	PF02991	0.660
LIG_LIR_Gen_1	235	244	PF02991	0.518
LIG_LIR_Gen_1	297	307	PF02991	0.665
LIG_LIR_Nem_3	166	170	PF02991	0.660
LIG_LIR_Nem_3	223	228	PF02991	0.660
LIG_LIR_Nem_3	235	239	PF02991	0.518
LIG_LIR_Nem_3	295	299	PF02991	0.559
LIG_Pex14_2	180	184	PF04695	0.660
LIG_Pex14_2	296	300	PF04695	0.660
LIG_SH2_CRK	225	229	PF00017	0.545
LIG_SH2_CRK	236	240	PF00017	0.545
LIG_SH2_NCK_1	225	229	PF00017	0.660
LIG_SH2_STAP1	225	229	PF00017	0.660
LIG_SH2_STAT3	229	232	PF00017	0.507
LIG_SH2_STAT5	225	228	PF00017	0.660
LIG_SH2_STAT5	247	250	PF00017	0.545
LIG_SH3_3	236	242	PF00018	0.660
LIG_SH3_3	245	251	PF00018	0.542
LIG_SH3_3	56	62	PF00018	0.660
LIG_SH3_3	87	93	PF00018	0.660
LIG_SUMO_SIM_anti_2	120	126	PF11976	0.548
LIG_SUMO_SIM_anti_2	14	21	PF11976	0.542
LIG_SUMO_SIM_anti_2	148	153	PF11976	0.660
LIG_SUMO_SIM_anti_2	27	32	PF11976	0.624
LIG_SUMO_SIM_par_1	29	35	PF11976	0.660
LIG_UBA3_1	218	227	PF00899	0.554
MOD_CK1_1	91	97	PF00069	0.301
MOD_GSK3_1	119	126	PF00069	0.554
MOD_GSK3_1	180	187	PF00069	0.554
MOD_GSK3_1	228	235	PF00069	0.304
MOD_GSK3_1	249	256	PF00069	0.525
MOD_GSK3_1	267	274	PF00069	0.257
MOD_GSK3_1	285	292	PF00069	0.260
MOD_GSK3_1	82	89	PF00069	0.315
MOD_GSK3_1	91	98	PF00069	0.298
MOD_N-GLC_1	159	164	PF02516	0.554
MOD_N-GLC_1	180	185	PF02516	0.554
MOD_N-GLC_1	38	43	PF02516	0.554
MOD_NEK2_1	152	157	PF00069	0.433
MOD_NEK2_1	191	196	PF00069	0.415
MOD_NEK2_1	294	299	PF00069	0.301
MOD_NEK2_1	31	36	PF00069	0.554
MOD_PIKK_1	192	198	PF00454	0.301
MOD_PIKK_1	228	234	PF00454	0.301
MOD_PIKK_1	285	291	PF00454	0.554
MOD_PKA_2	232	238	PF00069	0.364
MOD_Plk_1	180	186	PF00069	0.554
MOD_Plk_1	38	44	PF00069	0.554
MOD_Plk_4	184	190	PF00069	0.556
MOD_Plk_4	249	255	PF00069	0.425
MOD_Plk_4	32	38	PF00069	0.554
MOD_ProDKin_1	159	165	PF00069	0.554
MOD_ProDKin_1	197	203	PF00069	0.554
MOD_SUMO_rev_2	111	121	PF00179	0.224
MOD_SUMO_rev_2	91	98	PF00179	0.554
TRG_DiLeu_BaEn_2	165	171	PF01217	0.515
TRG_ENDOCYTIC_2	225	228	PF00928	0.393
TRG_ENDOCYTIC_2	236	239	PF00928	0.393
TRG_ENDOCYTIC_2	299	302	PF00928	0.290
TRG_NES_CRM1_1	264	277	PF08389	0.554
TRG_NLS_MonoExtN_4	70	77	PF00514	0.301

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IKH5	Leptomonas seymouri	35%	89%
A0A0S4ITJ9	Bodo saltans	30%	87%
A0A1X0NNR9	Trypanosomatidae	29%	87%
A0A422NEX0	Trypanosoma rangeli	25%	96%
A0L0Y0	Shewanella sp. (strain ANA-3)	39%	98%
A1A778	Escherichia coli O1:K1 / APEC	37%	100%
A1A8R9	Escherichia coli O1:K1 / APEC	36%	100%
A1AD20	Escherichia coli O1:K1 / APEC	41%	100%
A3D0C6	Shewanella baltica (strain OS155 / ATCC BAA-1091)	39%	98%
A4H7H4	Leishmania braziliensis	32%	100%
A4W829	Enterobacter sp. (strain 638)	37%	100%
A6T4G4	Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)	37%	100%
A6T9S2	Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)	35%	100%
A6WSI3	Shewanella baltica (strain OS185)	39%	98%
A7ZHB9	Escherichia coli O139:H28 (strain E24377A / ETEC)	37%	100%
A7ZJ42	Escherichia coli O139:H28 (strain E24377A / ETEC)	37%	100%
A7ZNY5	Escherichia coli O139:H28 (strain E24377A / ETEC)	41%	100%
A7ZVX8	Escherichia coli O9:H4 (strain HS)	37%	100%
A7ZXS0	Escherichia coli O9:H4 (strain HS)	37%	100%
A8A225	Escherichia coli O9:H4 (strain HS)	41%	100%
A8AJF8	Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)	35%	100%
A9MKC6	Salmonella arizonae (strain ATCC BAA-731 / CDC346-86 / RSK2980)	35%	100%
A9MUJ0	Salmonella paratyphi B (strain ATCC BAA-1250 / SPB7)	35%	100%
A9MYH1	Salmonella paratyphi B (strain ATCC BAA-1250 / SPB7)	37%	100%
B1IYA8	Escherichia coli (strain ATCC 8739 / DSM 1576 / NBRC 3972 / NCIMB 8545 / WDCM 00012 / Crooks)	41%	100%
B1IYF7	Escherichia coli (strain ATCC 8739 / DSM 1576 / NBRC 3972 / NCIMB 8545 / WDCM 00012 / Crooks)	37%	100%
B1KHA5	Shewanella woodyi (strain ATCC 51908 / MS32)	39%	100%
B1LFW0	Escherichia coli (strain SMS-3-5 / SECEC)	38%	100%
B1LKQ9	Escherichia coli (strain SMS-3-5 / SECEC)	41%	100%
B1LLA1	Escherichia coli (strain SMS-3-5 / SECEC)	37%	100%
B1X860	Escherichia coli (strain K12 / DH10B)	41%	100%
B1XBF5	Escherichia coli (strain K12 / DH10B)	37%	100%
B2TU69	Shigella boydii serotype 18 (strain CDC 3083-94 / BS512)	37%	100%
B2U249	Shigella boydii serotype 18 (strain CDC 3083-94 / BS512)	37%	100%
B4SYM0	Salmonella newport (strain SL254)	35%	100%
B4T6H8	Salmonella newport (strain SL254)	37%	100%
B4TB65	Salmonella heidelberg (strain SL476)	35%	100%
B4TIF1	Salmonella heidelberg (strain SL476)	37%	100%
B4TPY1	Salmonella schwarzengrund (strain CVM19633)	35%	100%
B4TWP3	Salmonella schwarzengrund (strain CVM19633)	37%	100%
B5BCD9	Salmonella paratyphi A (strain AKU_12601)	35%	100%
B5BLL5	Salmonella paratyphi A (strain AKU_12601)	37%	100%
B5EZA2	Salmonella agona (strain SL483)	35%	100%
B5F731	Salmonella agona (strain SL483)	39%	100%
B5FHE5	Salmonella dublin (strain CT_02021853)	37%	100%
B5FNA2	Salmonella dublin (strain CT_02021853)	35%	100%
B5QVR2	Salmonella enteritidis PT4 (strain P125109)	35%	100%
B5R1P1	Salmonella enteritidis PT4 (strain P125109)	39%	100%
B5R809	Salmonella gallinarum (strain 287/91 / NCTC 13346)	35%	100%
B5RG86	Salmonella gallinarum (strain 287/91 / NCTC 13346)	39%	100%
B5XWV7	Klebsiella pneumoniae (strain 342)	35%	100%
B5Y231	Klebsiella pneumoniae (strain 342)	37%	100%
B5YQK2	Escherichia coli O157:H7 (strain EC4115 / EHEC)	37%	100%
B5YWV3	Escherichia coli O157:H7 (strain EC4115 / EHEC)	41%	100%
B5YYC2	Escherichia coli O157:H7 (strain EC4115 / EHEC)	36%	100%
B6HYX8	Escherichia coli (strain SE11)	37%	100%
B6I163	Escherichia coli (strain SE11)	37%	100%
B6I8L3	Escherichia coli (strain SE11)	41%	100%
B7L4F2	Escherichia coli (strain 55989 / EAEC)	37%	100%
B7L9J7	Escherichia coli (strain 55989 / EAEC)	37%	100%
B7LAI5	Escherichia coli (strain 55989 / EAEC)	41%	100%
B7LKV4	Escherichia fergusonii (strain ATCC 35469 / DSM 13698 / CCUG 18766 / IAM 14443 / JCM 21226 / LMG 7866 / NBRC 102419 / NCTC 12128 / CDC 0568-73)	37%	100%
B7LVC3	Escherichia fergusonii (strain ATCC 35469 / DSM 13698 / CCUG 18766 / IAM 14443 / JCM 21226 / LMG 7866 / NBRC 102419 / NCTC 12128 / CDC 0568-73)	41%	100%
B7LVN6	Escherichia fergusonii (strain ATCC 35469 / DSM 13698 / CCUG 18766 / IAM 14443 / JCM 21226 / LMG 7866 / NBRC 102419 / NCTC 12128 / CDC 0568-73)	37%	100%
B7M0C6	Escherichia coli O8 (strain IAI1)	37%	100%
B7M510	Escherichia coli O8 (strain IAI1)	41%	100%
B7M5H8	Escherichia coli O8 (strain IAI1)	37%	100%
B7MAF0	Escherichia coli O45:K1 (strain S88 / ExPEC)	37%	100%
B7MF76	Escherichia coli O45:K1 (strain S88 / ExPEC)	41%	100%
B7MFR7	Escherichia coli O45:K1 (strain S88 / ExPEC)	36%	100%
B7MNN5	Escherichia coli O81 (strain ED1a)	37%	100%
B7MRT1	Escherichia coli O81 (strain ED1a)	37%	100%
B7MX26	Escherichia coli O81 (strain ED1a)	41%	100%
B7N5C5	Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC)	42%	100%
B7N7Q4	Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC)	37%	100%
B7N9Q7	Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC)	37%	100%
B7NHD4	Escherichia coli O7:K1 (strain IAI39 / ExPEC)	37%	100%
B7NM03	Escherichia coli O7:K1 (strain IAI39 / ExPEC)	37%	100%
B7NMX6	Escherichia coli O7:K1 (strain IAI39 / ExPEC)	41%	100%
B7UFH8	Escherichia coli O127:H6 (strain E2348/69 / EPEC)	41%	100%
B7UI75	Escherichia coli O127:H6 (strain E2348/69 / EPEC)	37%	100%
B7UKT4	Escherichia coli O127:H6 (strain E2348/69 / EPEC)	36%	100%
C0PW90	Salmonella paratyphi C (strain RKS4594)	35%	100%
C0Q4J2	Salmonella paratyphi C (strain RKS4594)	38%	100%
C4ZPV6	Escherichia coli (strain K12 / MC4100 / BW2952)	37%	100%
C4ZU12	Escherichia coli (strain K12 / MC4100 / BW2952)	41%	100%
C4ZWD8	Escherichia coli (strain K12 / MC4100 / BW2952)	37%	100%
C9ZT72	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	30%	88%
E9APK7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	28%	89%
E9ARU2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	85%	100%
P0C0W2	Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)	37%	100%
P22564	Escherichia coli (strain K12)	37%	100%
P33022	Escherichia coli (strain K12)	41%	100%
P41409	Escherichia coli (strain K12)	37%	100%
P83851	Leishmania major	82%	100%
Q04179	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	32%	92%
Q0HEZ4	Shewanella sp. (strain MR-4)	39%	98%
Q0HZ05	Shewanella sp. (strain MR-7)	39%	98%
Q0T2W2	Shigella flexneri serotype 5b (strain 8401)	41%	100%
Q0T6R1	Shigella flexneri serotype 5b (strain 8401)	37%	100%
Q0T8G4	Shigella flexneri serotype 5b (strain 8401)	37%	100%
Q0TFS8	Escherichia coli O6:K15:H31 (strain 536 / UPEC)	41%	100%
Q0TK29	Escherichia coli O6:K15:H31 (strain 536 / UPEC)	37%	100%
Q0TLW1	Escherichia coli O6:K15:H31 (strain 536 / UPEC)	37%	100%
Q10314	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	34%	95%
Q18L05	Leishmania infantum	28%	89%
Q18L65	Leishmania donovani	28%	89%
Q1R9R0	Escherichia coli (strain UTI89 / UPEC)	41%	100%
Q1RGH2	Escherichia coli (strain UTI89 / UPEC)	37%	100%
Q27546	Crithidia fasciculata	79%	99%
Q2PDC5	Leishmania infantum	84%	100%
Q31YW9	Shigella boydii serotype 4 (strain Sb227)	37%	100%
Q324P5	Shigella boydii serotype 4 (strain Sb227)	37%	100%
Q326J3	Shigella boydii serotype 4 (strain Sb227)	37%	100%
Q32ER0	Shigella dysenteriae serotype 1 (strain Sd197)	38%	100%
Q32K67	Shigella dysenteriae serotype 1 (strain Sd197)	37%	100%
Q3Z044	Shigella sonnei (strain Ss046)	41%	100%
Q3Z4E1	Shigella sonnei (strain Ss046)	37%	100%
Q3Z5Y0	Shigella sonnei (strain Ss046)	36%	100%
Q4QDN1	Leishmania major	82%	100%
Q4QFX2	Leishmania major	29%	100%
Q57RR6	Salmonella choleraesuis (strain SC-B67)	35%	100%
Q57TL0	Salmonella choleraesuis (strain SC-B67)	38%	100%
Q5PKI2	Salmonella paratyphi A (strain ATCC 9150 / SARB42)	37%	100%
Q5PM78	Salmonella paratyphi A (strain ATCC 9150 / SARB42)	35%	100%
Q652Q8	Oryza sativa subsp. japonica	36%	93%
Q6LQG2	Photobacterium profundum (strain SS9)	38%	100%
Q6ZJ05	Oryza sativa subsp. japonica	35%	97%
Q7KWM9	Dictyostelium discoideum	35%	92%
Q83KF1	Shigella flexneri	41%	100%
Q83LY1	Shigella flexneri	37%	100%
Q83MH0	Shigella flexneri	37%	100%
Q8EIM7	Shewanella oneidensis (strain MR-1)	38%	98%
Q8FFT8	Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)	41%	100%
Q8FJY8	Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)	36%	100%
Q8LAC4	Arabidopsis thaliana	33%	97%
Q8WQX2	Leishmania donovani	84%	100%
Q8XA41	Escherichia coli O157:H7	36%	100%
Q8XBL8	Escherichia coli O157:H7	37%	100%
Q8XE94	Escherichia coli O157:H7	41%	100%
Q8Z8H0	Salmonella typhi	35%	100%
Q8Z9M9	Salmonella typhi	38%	100%
Q8ZQY4	Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)	35%	100%
Q8ZRY7	Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)	37%	100%
Q9HGL1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	26%	80%
Q9P6J4	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	36%	100%
Q9SJM7	Arabidopsis thaliana	36%	93%
V5DDY4	Trypanosoma cruzi	29%	88%