RING-type E3 ubiquitin transferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Zinc finger, C3HC4 type (RING finger), putative

Species:

Leishmania braziliensis

UniProt:

A4H9M5_LEIBR

TriTrypDb:

LbrM.18.1250 , LBRM2903_180018000

Length:

333

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 2
Forrest at al. (procyclic)	no	yes: 2
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 9

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 5

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 5

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4H9M5

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H9M5

Function

Biological processes
Term	Name	Level	Count
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0016567	protein ubiquitination	7	1
GO:0019538	protein metabolic process	3	1
GO:0032446	protein modification by small protein conjugation	6	1
GO:0036211	protein modification process	4	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0044238	primary metabolic process	2	1
GO:0070647	protein modification by small protein conjugation or removal	5	1
GO:0071704	organic substance metabolic process	2	1
GO:1901564	organonitrogen compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0004842	ubiquitin-protein transferase activity	4	12
GO:0005488	binding	1	12
GO:0016740	transferase activity	2	12
GO:0019787	ubiquitin-like protein transferase activity	3	12
GO:0043167	ion binding	2	12
GO:0043169	cation binding	3	12
GO:0046872	metal ion binding	4	12
GO:0061630	ubiquitin protein ligase activity	5	12
GO:0061659	ubiquitin-like protein ligase activity	4	12
GO:0140096	catalytic activity, acting on a protein	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	149	151	PF00675	0.538
CLV_NRD_NRD_1	221	223	PF00675	0.406
CLV_PCSK_KEX2_1	149	151	PF00082	0.326
CLV_PCSK_KEX2_1	190	192	PF00082	0.572
CLV_PCSK_KEX2_1	220	222	PF00082	0.437
CLV_PCSK_PC1ET2_1	190	192	PF00082	0.572
CLV_PCSK_PC1ET2_1	220	222	PF00082	0.455
CLV_PCSK_SKI1_1	149	153	PF00082	0.490
DOC_MAPK_gen_1	149	157	PF00069	0.533
DOC_MAPK_MEF2A_6	149	157	PF00069	0.454
DOC_MAPK_MEF2A_6	173	180	PF00069	0.317
DOC_MAPK_NFAT4_5	150	158	PF00069	0.533
DOC_PP2B_LxvP_1	319	322	PF13499	0.394
DOC_USP7_MATH_1	230	234	PF00917	0.381
DOC_USP7_MATH_1	251	255	PF00917	0.495
DOC_USP7_UBL2_3	165	169	PF12436	0.397
DOC_WW_Pin1_4	246	251	PF00397	0.549
DOC_WW_Pin1_4	42	47	PF00397	0.636
LIG_14-3-3_CanoR_1	149	154	PF00244	0.458
LIG_14-3-3_CanoR_1	25	30	PF00244	0.609
LIG_14-3-3_CanoR_1	312	322	PF00244	0.266
LIG_BIR_II_1	1	5	PF00653	0.492
LIG_FHA_1	108	114	PF00498	0.341
LIG_FHA_1	150	156	PF00498	0.470
LIG_FHA_1	183	189	PF00498	0.483
LIG_FHA_1	93	99	PF00498	0.389
LIG_FHA_2	209	215	PF00498	0.329
LIG_FHA_2	262	268	PF00498	0.684
LIG_FHA_2	62	68	PF00498	0.622
LIG_SH2_GRB2like	19	22	PF00017	0.682
LIG_SH2_SRC	111	114	PF00017	0.357
LIG_SH2_SRC	91	94	PF00017	0.461
LIG_SH2_STAT3	171	174	PF00017	0.382
LIG_SH2_STAT5	101	104	PF00017	0.515
LIG_SH2_STAT5	91	94	PF00017	0.529
LIG_SH3_1	303	309	PF00018	0.281
LIG_SH3_3	127	133	PF00018	0.398
LIG_SH3_3	303	309	PF00018	0.266
LIG_TRAF2_1	159	162	PF00917	0.493
LIG_WRC_WIRS_1	97	102	PF05994	0.412
MOD_CDK_SPxxK_3	246	253	PF00069	0.481
MOD_CK1_1	233	239	PF00069	0.410
MOD_CK1_1	28	34	PF00069	0.665
MOD_CK1_1	325	331	PF00069	0.643
MOD_CK1_1	54	60	PF00069	0.673
MOD_CK1_1	87	93	PF00069	0.491
MOD_CK2_1	156	162	PF00069	0.452
MOD_CK2_1	260	266	PF00069	0.700
MOD_CK2_1	61	67	PF00069	0.622
MOD_GlcNHglycan	232	235	PF01048	0.342
MOD_GlcNHglycan	253	256	PF01048	0.612
MOD_GlcNHglycan	30	33	PF01048	0.525
MOD_GSK3_1	101	108	PF00069	0.425
MOD_GSK3_1	21	28	PF00069	0.564
MOD_GSK3_1	222	229	PF00069	0.351
MOD_GSK3_1	245	252	PF00069	0.757
MOD_GSK3_1	30	37	PF00069	0.717
MOD_GSK3_1	325	332	PF00069	0.596
MOD_GSK3_1	80	87	PF00069	0.428
MOD_GSK3_1	92	99	PF00069	0.386
MOD_N-GLC_1	21	26	PF02516	0.699
MOD_N-GLC_1	325	330	PF02516	0.459
MOD_N-GLC_1	34	39	PF02516	0.732
MOD_N-GLC_1	59	64	PF02516	0.562
MOD_NEK2_1	100	105	PF00069	0.353
MOD_NEK2_1	260	265	PF00069	0.587
MOD_PIKK_1	222	228	PF00454	0.377
MOD_PIKK_1	92	98	PF00454	0.496
MOD_PKA_1	149	155	PF00069	0.537
MOD_PKA_2	149	155	PF00069	0.388
MOD_PKA_2	51	57	PF00069	0.529
MOD_Plk_1	208	214	PF00069	0.329
MOD_Plk_1	84	90	PF00069	0.426
MOD_Plk_2-3	107	113	PF00069	0.459
MOD_Plk_2-3	156	162	PF00069	0.537
MOD_Plk_4	314	320	PF00069	0.281
MOD_Plk_4	87	93	PF00069	0.498
MOD_Plk_4	96	102	PF00069	0.454
MOD_ProDKin_1	246	252	PF00069	0.551
MOD_ProDKin_1	42	48	PF00069	0.635
MOD_SUMO_for_1	133	136	PF00179	0.458
MOD_SUMO_for_1	138	141	PF00179	0.450
TRG_ER_diArg_1	149	151	PF00400	0.478
TRG_ER_diArg_1	221	223	PF00400	0.383
TRG_NLS_MonoExtN_4	218	224	PF00514	0.431
TRG_Pf-PMV_PEXEL_1	222	226	PF00026	0.378

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IMI8	Leptomonas seymouri	73%	90%
A0A0S4JNW6	Bodo saltans	34%	87%
A0A1X0P805	Trypanosomatidae	61%	90%
A0A3S7WV37	Leishmania donovani	79%	91%
A0A422P0Q0	Trypanosoma rangeli	55%	94%
A4HXY9	Leishmania infantum	79%	91%
D0A5B8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	53%	100%
E9ARQ2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	79%	92%
Q4QDS7	Leishmania major	80%	100%
V5BNN3	Trypanosoma cruzi	54%	94%