Putative methyltransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Multisite-specific tRNA:(cytosine-C(5))-methyltransferase, putative

Species:

Leishmania braziliensis

UniProt:

A4H9M1_LEIBR

TriTrypDb:

LbrM.18.1210 , LBRM2903_180017600

Length:

837

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0005730	nucleolus	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4H9M1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H9M1

Function

Biological processes
Term	Name	Level	Count
GO:0001510	RNA methylation	4	12
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009451	RNA modification	5	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0032259	methylation	2	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043412	macromolecule modification	4	12
GO:0043414	macromolecule methylation	3	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044260	obsolete cellular macromolecule metabolic process	3	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	12
GO:0003723	RNA binding	4	12
GO:0003824	catalytic activity	1	12
GO:0005488	binding	1	12
GO:0008168	methyltransferase activity	4	12
GO:0016740	transferase activity	2	12
GO:0016741	transferase activity, transferring one-carbon groups	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	28	32	PF00656	0.350
CLV_C14_Caspase3-7	59	63	PF00656	0.643
CLV_C14_Caspase3-7	64	68	PF00656	0.551
CLV_MEL_PAP_1	677	683	PF00089	0.447
CLV_NRD_NRD_1	11	13	PF00675	0.570
CLV_NRD_NRD_1	133	135	PF00675	0.567
CLV_NRD_NRD_1	186	188	PF00675	0.659
CLV_NRD_NRD_1	3	5	PF00675	0.635
CLV_NRD_NRD_1	399	401	PF00675	0.240
CLV_NRD_NRD_1	527	529	PF00675	0.248
CLV_NRD_NRD_1	609	611	PF00675	0.380
CLV_NRD_NRD_1	674	676	PF00675	0.374
CLV_NRD_NRD_1	7	9	PF00675	0.602
CLV_NRD_NRD_1	99	101	PF00675	0.477
CLV_PCSK_FUR_1	345	349	PF00082	0.260
CLV_PCSK_KEX2_1	132	134	PF00082	0.562
CLV_PCSK_KEX2_1	2	4	PF00082	0.636
CLV_PCSK_KEX2_1	347	349	PF00082	0.260
CLV_PCSK_KEX2_1	609	611	PF00082	0.380
CLV_PCSK_KEX2_1	7	9	PF00082	0.581
CLV_PCSK_KEX2_1	99	101	PF00082	0.477
CLV_PCSK_PC1ET2_1	2	4	PF00082	0.655
CLV_PCSK_PC1ET2_1	347	349	PF00082	0.260
CLV_PCSK_PC7_1	3	9	PF00082	0.657
CLV_PCSK_SKI1_1	224	228	PF00082	0.477
CLV_PCSK_SKI1_1	325	329	PF00082	0.335
CLV_PCSK_SKI1_1	422	426	PF00082	0.268
CLV_PCSK_SKI1_1	497	501	PF00082	0.220
CLV_PCSK_SKI1_1	528	532	PF00082	0.499
CLV_PCSK_SKI1_1	610	614	PF00082	0.357
CLV_PCSK_SKI1_1	704	708	PF00082	0.500
CLV_PCSK_SKI1_1	77	81	PF00082	0.400
DEG_Nend_UBRbox_1	1	4	PF02207	0.685
DEG_ODPH_VHL_1	668	681	PF01847	0.463
DOC_CKS1_1	201	206	PF01111	0.466
DOC_CKS1_1	226	231	PF01111	0.540
DOC_CKS1_1	553	558	PF01111	0.280
DOC_CKS1_1	613	618	PF01111	0.458
DOC_CYCLIN_RxL_1	422	434	PF00134	0.473
DOC_CYCLIN_RxL_1	671	682	PF00134	0.489
DOC_CYCLIN_RxL_1	74	84	PF00134	0.278
DOC_CYCLIN_yCln2_LP_2	495	501	PF00134	0.493
DOC_CYCLIN_yCln2_LP_2	553	559	PF00134	0.504
DOC_CYCLIN_yCln2_LP_2	613	619	PF00134	0.465
DOC_MAPK_gen_1	550	559	PF00069	0.547
DOC_MAPK_gen_1	626	635	PF00069	0.513
DOC_MAPK_HePTP_8	623	635	PF00069	0.554
DOC_MAPK_MEF2A_6	626	635	PF00069	0.492
DOC_PP1_RVXF_1	673	680	PF00149	0.505
DOC_PP4_FxxP_1	667	670	PF00568	0.515
DOC_USP7_MATH_1	178	182	PF00917	0.621
DOC_USP7_MATH_1	240	244	PF00917	0.493
DOC_USP7_MATH_1	56	60	PF00917	0.750
DOC_USP7_MATH_1	586	590	PF00917	0.546
DOC_USP7_MATH_1	63	67	PF00917	0.504
DOC_USP7_UBL2_3	184	188	PF12436	0.413
DOC_USP7_UBL2_3	333	337	PF12436	0.449
DOC_USP7_UBL2_3	721	725	PF12436	0.615
DOC_WW_Pin1_4	165	170	PF00397	0.542
DOC_WW_Pin1_4	200	205	PF00397	0.377
DOC_WW_Pin1_4	225	230	PF00397	0.512
DOC_WW_Pin1_4	486	491	PF00397	0.450
DOC_WW_Pin1_4	552	557	PF00397	0.300
DOC_WW_Pin1_4	612	617	PF00397	0.438
DOC_WW_Pin1_4	826	831	PF00397	0.759
LIG_14-3-3_CanoR_1	100	108	PF00244	0.451
LIG_14-3-3_CanoR_1	132	141	PF00244	0.611
LIG_14-3-3_CanoR_1	210	218	PF00244	0.454
LIG_14-3-3_CanoR_1	224	229	PF00244	0.476
LIG_14-3-3_CanoR_1	266	276	PF00244	0.444
LIG_14-3-3_CanoR_1	335	343	PF00244	0.449
LIG_14-3-3_CanoR_1	403	413	PF00244	0.484
LIG_14-3-3_CanoR_1	575	583	PF00244	0.453
LIG_14-3-3_CanoR_1	813	817	PF00244	0.609
LIG_Actin_WH2_2	467	484	PF00022	0.427
LIG_Actin_WH2_2	594	611	PF00022	0.290
LIG_APCC_ABBA_1	458	463	PF00400	0.377
LIG_APCC_ABBA_1	512	517	PF00400	0.449
LIG_BRCT_BRCA1_1	663	667	PF00533	0.486
LIG_CSL_BTD_1	553	556	PF09270	0.290
LIG_deltaCOP1_diTrp_1	191	197	PF00928	0.400
LIG_deltaCOP1_diTrp_1	560	570	PF00928	0.366
LIG_deltaCOP1_diTrp_1	67	71	PF00928	0.303
LIG_Dynein_DLC8_1	335	341	PF01221	0.489
LIG_FAT_LD_1	75	83	PF03623	0.501
LIG_FHA_1	143	149	PF00498	0.480
LIG_FHA_1	206	212	PF00498	0.369
LIG_FHA_1	221	227	PF00498	0.409
LIG_FHA_1	337	343	PF00498	0.483
LIG_FHA_1	432	438	PF00498	0.519
LIG_FHA_1	613	619	PF00498	0.432
LIG_FHA_1	737	743	PF00498	0.511
LIG_FHA_1	96	102	PF00498	0.566
LIG_FHA_2	225	231	PF00498	0.539
LIG_FHA_2	257	263	PF00498	0.312
LIG_FHA_2	293	299	PF00498	0.509
LIG_FHA_2	355	361	PF00498	0.473
LIG_FHA_2	41	47	PF00498	0.623
LIG_LIR_Apic_2	625	631	PF02991	0.461
LIG_LIR_Apic_2	664	670	PF02991	0.454
LIG_LIR_Gen_1	191	202	PF02991	0.363
LIG_LIR_Gen_1	275	284	PF02991	0.412
LIG_LIR_Gen_1	381	392	PF02991	0.449
LIG_LIR_Gen_1	561	571	PF02991	0.393
LIG_LIR_Gen_1	89	97	PF02991	0.560
LIG_LIR_Nem_3	155	160	PF02991	0.423
LIG_LIR_Nem_3	191	197	PF02991	0.400
LIG_LIR_Nem_3	275	279	PF02991	0.421
LIG_LIR_Nem_3	362	367	PF02991	0.427
LIG_LIR_Nem_3	381	387	PF02991	0.449
LIG_LIR_Nem_3	561	567	PF02991	0.358
LIG_LIR_Nem_3	569	573	PF02991	0.361
LIG_LIR_Nem_3	578	584	PF02991	0.379
LIG_LIR_Nem_3	655	661	PF02991	0.351
LIG_LIR_Nem_3	701	706	PF02991	0.496
LIG_LIR_Nem_3	804	808	PF02991	0.498
LIG_LIR_Nem_3	89	95	PF02991	0.554
LIG_MYND_1	498	502	PF01753	0.554
LIG_NRBOX	729	735	PF00104	0.613
LIG_NRBOX	74	80	PF00104	0.499
LIG_Pex14_1	364	368	PF04695	0.473
LIG_Pex14_1	409	413	PF04695	0.449
LIG_SH2_CRK	349	353	PF00017	0.473
LIG_SH2_CRK	628	632	PF00017	0.479
LIG_SH2_NCK_1	584	588	PF00017	0.376
LIG_SH2_STAT5	263	266	PF00017	0.426
LIG_SH2_STAT5	35	38	PF00017	0.466
LIG_SH2_STAT5	368	371	PF00017	0.524
LIG_SH2_STAT5	413	416	PF00017	0.449
LIG_SH2_STAT5	696	699	PF00017	0.528
LIG_SH3_3	231	237	PF00018	0.434
LIG_SH3_3	369	375	PF00018	0.500
LIG_SH3_3	495	501	PF00018	0.512
LIG_SH3_3	527	533	PF00018	0.509
LIG_SUMO_SIM_anti_2	462	469	PF11976	0.449
LIG_SUMO_SIM_anti_2	710	715	PF11976	0.390
LIG_SUMO_SIM_anti_2	738	744	PF11976	0.536
LIG_SUMO_SIM_anti_2	76	82	PF11976	0.521
LIG_SUMO_SIM_par_1	283	289	PF11976	0.270
LIG_SUMO_SIM_par_1	610	615	PF11976	0.280
LIG_SUMO_SIM_par_1	738	744	PF11976	0.567
LIG_SUMO_SIM_par_1	78	85	PF11976	0.444
LIG_TRAF2_1	180	183	PF00917	0.440
LIG_TRAF2_1	275	278	PF00917	0.364
LIG_TRAF2_1	744	747	PF00917	0.614
LIG_TRAF2_1	830	833	PF00917	0.788
LIG_TRFH_1	599	603	PF08558	0.423
LIG_UBA3_1	22	30	PF00899	0.324
LIG_WRC_WIRS_1	567	572	PF05994	0.474
LIG_WRC_WIRS_1	802	807	PF05994	0.459
LIG_WW_1	501	504	PF00397	0.554
MOD_CDK_SPxxK_3	165	172	PF00069	0.584
MOD_CK1_1	104	110	PF00069	0.415
MOD_CK1_1	121	127	PF00069	0.496
MOD_CK1_1	272	278	PF00069	0.363
MOD_CK1_1	404	410	PF00069	0.475
MOD_CK1_1	441	447	PF00069	0.449
MOD_CK1_1	577	583	PF00069	0.436
MOD_CK1_1	738	744	PF00069	0.620
MOD_CK2_1	256	262	PF00069	0.400
MOD_CK2_1	272	278	PF00069	0.372
MOD_CK2_1	41	47	PF00069	0.618
MOD_CK2_1	70	76	PF00069	0.519
MOD_CK2_1	725	731	PF00069	0.631
MOD_CK2_1	741	747	PF00069	0.503
MOD_CK2_1	751	757	PF00069	0.485
MOD_CK2_1	820	826	PF00069	0.668
MOD_GlcNHglycan	123	126	PF01048	0.606
MOD_GlcNHglycan	244	247	PF01048	0.507
MOD_GlcNHglycan	269	272	PF01048	0.433
MOD_GlcNHglycan	280	283	PF01048	0.342
MOD_GlcNHglycan	315	318	PF01048	0.307
MOD_GlcNHglycan	319	322	PF01048	0.283
MOD_GlcNHglycan	393	396	PF01048	0.249
MOD_GlcNHglycan	409	412	PF01048	0.249
MOD_GlcNHglycan	57	61	PF01048	0.693
MOD_GlcNHglycan	590	593	PF01048	0.522
MOD_GlcNHglycan	663	666	PF01048	0.374
MOD_GlcNHglycan	72	75	PF01048	0.334
MOD_GlcNHglycan	725	728	PF01048	0.393
MOD_GlcNHglycan	780	783	PF01048	0.363
MOD_GlcNHglycan	784	787	PF01048	0.407
MOD_GlcNHglycan	822	825	PF01048	0.726
MOD_GSK3_1	103	110	PF00069	0.410
MOD_GSK3_1	220	227	PF00069	0.528
MOD_GSK3_1	242	249	PF00069	0.512
MOD_GSK3_1	313	320	PF00069	0.504
MOD_GSK3_1	370	377	PF00069	0.536
MOD_GSK3_1	391	398	PF00069	0.449
MOD_GSK3_1	401	408	PF00069	0.449
MOD_GSK3_1	409	416	PF00069	0.449
MOD_GSK3_1	732	739	PF00069	0.564
MOD_GSK3_1	778	785	PF00069	0.338
MOD_LATS_1	130	136	PF00433	0.675
MOD_N-GLC_1	470	475	PF02516	0.235
MOD_N-GLC_1	533	538	PF02516	0.343
MOD_NEK2_1	118	123	PF00069	0.549
MOD_NEK2_1	142	147	PF00069	0.546
MOD_NEK2_1	40	45	PF00069	0.486
MOD_NEK2_1	431	436	PF00069	0.491
MOD_NEK2_1	438	443	PF00069	0.433
MOD_NEK2_1	650	655	PF00069	0.547
MOD_NEK2_1	679	684	PF00069	0.497
MOD_NEK2_1	723	728	PF00069	0.452
MOD_NEK2_1	780	785	PF00069	0.354
MOD_NEK2_2	395	400	PF00069	0.449
MOD_NEK2_2	652	657	PF00069	0.423
MOD_NEK2_2	801	806	PF00069	0.512
MOD_PIKK_1	101	107	PF00454	0.480
MOD_PIKK_1	118	124	PF00454	0.475
MOD_PIKK_1	336	342	PF00454	0.477
MOD_PIKK_1	359	365	PF00454	0.394
MOD_PIKK_1	417	423	PF00454	0.449
MOD_PIKK_1	732	738	PF00454	0.629
MOD_PIKK_1	751	757	PF00454	0.556
MOD_PK_1	401	407	PF00069	0.509
MOD_PKA_1	132	138	PF00069	0.677
MOD_PKA_2	132	138	PF00069	0.677
MOD_PKA_2	152	158	PF00069	0.554
MOD_PKA_2	574	580	PF00069	0.281
MOD_PKA_2	679	685	PF00069	0.436
MOD_PKA_2	812	818	PF00069	0.707
MOD_PKA_2	95	101	PF00069	0.548
MOD_Plk_1	401	407	PF00069	0.509
MOD_Plk_1	470	476	PF00069	0.410
MOD_Plk_1	577	583	PF00069	0.472
MOD_Plk_4	283	289	PF00069	0.306
MOD_Plk_4	292	298	PF00069	0.458
MOD_Plk_4	409	415	PF00069	0.460
MOD_Plk_4	536	542	PF00069	0.569
MOD_Plk_4	566	572	PF00069	0.435
MOD_Plk_4	663	669	PF00069	0.394
MOD_Plk_4	738	744	PF00069	0.504
MOD_Plk_4	801	807	PF00069	0.485
MOD_ProDKin_1	165	171	PF00069	0.539
MOD_ProDKin_1	200	206	PF00069	0.377
MOD_ProDKin_1	225	231	PF00069	0.505
MOD_ProDKin_1	486	492	PF00069	0.450
MOD_ProDKin_1	552	558	PF00069	0.298
MOD_ProDKin_1	612	618	PF00069	0.436
MOD_ProDKin_1	826	832	PF00069	0.760
MOD_SUMO_rev_2	181	186	PF00179	0.666
MOD_SUMO_rev_2	5	15	PF00179	0.457
MOD_SUMO_rev_2	644	651	PF00179	0.410
MOD_SUMO_rev_2	827	837	PF00179	0.534
TRG_DiLeu_BaEn_1	747	752	PF01217	0.608
TRG_DiLeu_BaEn_1	76	81	PF01217	0.444
TRG_DiLeu_BaEn_2	192	198	PF01217	0.419
TRG_DiLeu_BaEn_2	662	668	PF01217	0.484
TRG_DiLeu_LyEn_5	144	149	PF01217	0.411
TRG_ER_diArg_1	132	134	PF00400	0.562
TRG_ER_diArg_1	608	610	PF00400	0.398
TRG_ER_diArg_1	623	626	PF00400	0.393
TRG_ER_diArg_1	7	10	PF00400	0.612
TRG_ER_diArg_1	99	101	PF00400	0.497
TRG_Pf-PMV_PEXEL_1	386	390	PF00026	0.273
TRG_Pf-PMV_PEXEL_1	685	689	PF00026	0.488

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P8H1	Leptomonas seymouri	69%	97%
A0A0S4KJ12	Bodo saltans	42%	100%
A0A1X0P6Y3	Trypanosomatidae	54%	100%
A0A3R7M922	Trypanosoma rangeli	52%	100%
A0A3S7WV22	Leishmania donovani	83%	99%
A4HXY5	Leishmania infantum	84%	99%
D0A386	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	26%	100%
D0A5C2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	50%	100%
E9ARP8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	84%	99%
O13935	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	27%	100%
P38205	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	28%	100%
Q4QDT1	Leishmania major	83%	100%
V5DFH6	Trypanosoma cruzi	52%	100%