Putative DNA-directed RNA polymerase II

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

DNA-directed RNA polymerase I subunit RPB5z, putative

Species:

Leishmania braziliensis

UniProt:

A4H9J0_LEIBR

TriTrypDb:

LbrM.18.0880 , LBRM2903_180014200

Length:

322

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 15
NetGPI	no	yes: 0, no: 15

Cellular components
Term	Name	Level	Count
GO:0000428	DNA-directed RNA polymerase complex	4	16
GO:0030880	RNA polymerase complex	3	16
GO:0032991	protein-containing complex	1	16
GO:0055029	nuclear DNA-directed RNA polymerase complex	3	14
GO:0061695	transferase complex, transferring phosphorus-containing groups	4	16
GO:0140513	nuclear protein-containing complex	2	14
GO:0140535	intracellular protein-containing complex	2	16
GO:1902494	catalytic complex	2	16
GO:1990234	transferase complex	3	16
GO:0005634	nucleus	5	2
GO:0005665	RNA polymerase II, core complex	4	2
GO:0005666	RNA polymerase III complex	4	2
GO:0005736	RNA polymerase I complex	4	2
GO:0005737	cytoplasm	2	1
GO:0031974	membrane-enclosed lumen	2	1
GO:0031981	nuclear lumen	5	1
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	1
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0043233	organelle lumen	3	1
GO:0070013	intracellular organelle lumen	4	1
GO:0097014	ciliary plasm	5	1
GO:0099568	cytoplasmic region	3	1
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

A4H9J0

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H9J0

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	16
GO:0006351	DNA-templated transcription	7	16
GO:0006725	cellular aromatic compound metabolic process	3	16
GO:0006807	nitrogen compound metabolic process	2	16
GO:0008152	metabolic process	1	16
GO:0009058	biosynthetic process	2	16
GO:0009059	macromolecule biosynthetic process	4	16
GO:0009987	cellular process	1	16
GO:0016070	RNA metabolic process	5	16
GO:0018130	heterocycle biosynthetic process	4	16
GO:0019438	aromatic compound biosynthetic process	4	16
GO:0032774	RNA biosynthetic process	5	16
GO:0034641	cellular nitrogen compound metabolic process	3	16
GO:0034654	nucleobase-containing compound biosynthetic process	4	16
GO:0043170	macromolecule metabolic process	3	16
GO:0044237	cellular metabolic process	2	16
GO:0044238	primary metabolic process	2	16
GO:0044249	cellular biosynthetic process	3	16
GO:0044271	cellular nitrogen compound biosynthetic process	4	16
GO:0046483	heterocycle metabolic process	3	16
GO:0071704	organic substance metabolic process	2	16
GO:0090304	nucleic acid metabolic process	4	16
GO:0097659	nucleic acid-templated transcription	6	16
GO:1901360	organic cyclic compound metabolic process	3	16
GO:1901362	organic cyclic compound biosynthetic process	4	16
GO:1901576	organic substance biosynthetic process	3	16

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	16
GO:0003677	DNA binding	4	16
GO:0003824	catalytic activity	1	16
GO:0003899	DNA-directed 5'-3' RNA polymerase activity	6	16
GO:0005488	binding	1	16
GO:0016740	transferase activity	2	16
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	16
GO:0016779	nucleotidyltransferase activity	4	16
GO:0034062	5'-3' RNA polymerase activity	5	16
GO:0097159	organic cyclic compound binding	2	16
GO:0097747	RNA polymerase activity	4	16
GO:0140098	catalytic activity, acting on RNA	3	16
GO:0140640	catalytic activity, acting on a nucleic acid	2	16
GO:1901363	heterocyclic compound binding	2	16
GO:0003887	DNA-directed DNA polymerase activity	5	1
GO:0034061	DNA polymerase activity	4	1
GO:0140097	catalytic activity, acting on DNA	3	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	114	116	PF00675	0.559
CLV_NRD_NRD_1	137	139	PF00675	0.773
CLV_NRD_NRD_1	274	276	PF00675	0.353
CLV_NRD_NRD_1	87	89	PF00675	0.599
CLV_PCSK_KEX2_1	114	116	PF00082	0.541
CLV_PCSK_KEX2_1	137	139	PF00082	0.773
CLV_PCSK_KEX2_1	87	89	PF00082	0.599
CLV_PCSK_SKI1_1	170	174	PF00082	0.587
CLV_PCSK_SKI1_1	197	201	PF00082	0.475
CLV_PCSK_SKI1_1	88	92	PF00082	0.656
DOC_CKS1_1	62	67	PF01111	0.388
DOC_MAPK_DCC_7	137	145	PF00069	0.496
DOC_MAPK_gen_1	137	145	PF00069	0.675
DOC_MAPK_gen_1	170	180	PF00069	0.470
DOC_MAPK_gen_1	200	209	PF00069	0.432
DOC_MAPK_MEF2A_6	137	145	PF00069	0.496
DOC_MAPK_MEF2A_6	173	180	PF00069	0.417
DOC_MAPK_MEF2A_6	212	219	PF00069	0.591
DOC_MAPK_RevD_3	261	276	PF00069	0.481
DOC_USP7_MATH_1	159	163	PF00917	0.598
DOC_USP7_MATH_1	68	72	PF00917	0.591
DOC_USP7_MATH_1	74	78	PF00917	0.654
DOC_USP7_UBL2_3	144	148	PF12436	0.606
DOC_USP7_UBL2_3	185	189	PF12436	0.385
DOC_USP7_UBL2_3	197	201	PF12436	0.361
DOC_USP7_UBL2_3	44	48	PF12436	0.343
DOC_WW_Pin1_4	180	185	PF00397	0.471
DOC_WW_Pin1_4	261	266	PF00397	0.513
DOC_WW_Pin1_4	61	66	PF00397	0.493
LIG_14-3-3_CanoR_1	129	135	PF00244	0.506
LIG_14-3-3_CanoR_1	14	20	PF00244	0.363
LIG_APCC_ABBA_1	239	244	PF00400	0.407
LIG_BIR_II_1	1	5	PF00653	0.503
LIG_deltaCOP1_diTrp_1	53	61	PF00928	0.418
LIG_FHA_1	129	135	PF00498	0.578
LIG_FHA_1	208	214	PF00498	0.460
LIG_FHA_2	230	236	PF00498	0.529
LIG_FHA_2	70	76	PF00498	0.556
LIG_LIR_Gen_1	120	128	PF02991	0.493
LIG_LIR_Nem_3	312	318	PF02991	0.487
LIG_Pex14_2	57	61	PF04695	0.394
LIG_SH2_SRC	103	106	PF00017	0.452
LIG_SH2_STAP1	103	107	PF00017	0.577
LIG_SH2_STAT5	28	31	PF00017	0.385
LIG_SH2_STAT5	294	297	PF00017	0.450
LIG_SH3_3	37	43	PF00018	0.472
LIG_SH3_3	59	65	PF00018	0.489
LIG_SUMO_SIM_par_1	204	210	PF11976	0.374
LIG_TRAF2_1	3	6	PF00917	0.450
LIG_TYR_ITIM	292	297	PF00017	0.461
MOD_CDK_SPK_2	180	185	PF00069	0.420
MOD_CK1_1	77	83	PF00069	0.760
MOD_CK2_1	184	190	PF00069	0.420
MOD_CK2_1	229	235	PF00069	0.521
MOD_CK2_1	261	267	PF00069	0.483
MOD_CK2_1	69	75	PF00069	0.485
MOD_GlcNHglycan	223	227	PF01048	0.483
MOD_GlcNHglycan	311	314	PF01048	0.301
MOD_GlcNHglycan	33	36	PF01048	0.434
MOD_GlcNHglycan	75	79	PF01048	0.624
MOD_GlcNHglycan	83	86	PF01048	0.698
MOD_GSK3_1	180	187	PF00069	0.440
MOD_GSK3_1	309	316	PF00069	0.521
MOD_GSK3_1	77	84	PF00069	0.643
MOD_N-GLC_1	277	282	PF02516	0.271
MOD_NEK2_1	128	133	PF00069	0.473
MOD_NEK2_1	179	184	PF00069	0.377
MOD_NEK2_1	277	282	PF00069	0.422
MOD_NEK2_2	184	189	PF00069	0.533
MOD_PIKK_1	277	283	PF00454	0.401
MOD_PKA_1	201	207	PF00069	0.427
MOD_PKA_2	128	134	PF00069	0.519
MOD_PKA_2	298	304	PF00069	0.475
MOD_PKA_2	69	75	PF00069	0.596
MOD_Plk_1	277	283	PF00069	0.471
MOD_Plk_4	15	21	PF00069	0.374
MOD_Plk_4	202	208	PF00069	0.423
MOD_Plk_4	298	304	PF00069	0.461
MOD_Plk_4	313	319	PF00069	0.461
MOD_ProDKin_1	180	186	PF00069	0.464
MOD_ProDKin_1	261	267	PF00069	0.513
MOD_ProDKin_1	61	67	PF00069	0.501
MOD_SUMO_for_1	143	146	PF00179	0.744
MOD_SUMO_for_1	90	93	PF00179	0.787
MOD_SUMO_rev_2	2	10	PF00179	0.429
MOD_SUMO_rev_2	93	100	PF00179	0.757
TRG_DiLeu_BaEn_2	266	272	PF01217	0.492
TRG_ENDOCYTIC_2	294	297	PF00928	0.481
TRG_ENDOCYTIC_2	318	321	PF00928	0.481
TRG_ER_diArg_1	113	115	PF00400	0.597
TRG_ER_diArg_1	136	138	PF00400	0.591
TRG_ER_diArg_1	86	88	PF00400	0.600
TRG_NLS_MonoExtN_4	197	204	PF00514	0.503
TRG_Pf-PMV_PEXEL_1	284	289	PF00026	0.260
TRG_Pf-PMV_PEXEL_1	88	93	PF00026	0.524

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P8I4	Leptomonas seymouri	67%	100%
A0A0S4J1J5	Bodo saltans	34%	100%
A0A1X0P763	Trypanosomatidae	46%	100%
A0A3Q8ID21	Leishmania donovani	35%	100%
A0A3R7KXJ2	Trypanosoma rangeli	47%	100%
A0A3S5H734	Leishmania donovani	78%	94%
A4HXV2	Leishmania infantum	35%	100%
A4HXV3	Leishmania infantum	79%	94%
C9ZZV0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	33%	100%
E9ARL5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	35%	100%
E9ARL6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	79%	94%
Q4QDW3	Leishmania major	78%	100%
Q4QDW4	Leishmania major	35%	100%
V5B6J4	Trypanosoma cruzi	46%	100%