Putative GPI-anchor transamidase subunit 8 (GPI8)

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

GPI-anchor transamidase subunit 8 (GPI8), putative

Species:

Leishmania braziliensis

UniProt:

A4H9E0_LEIBR

TriTrypDb:

LbrM.18.0380 , LBRM2903_180008800

Length:

357

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 6, no: 4
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0008303	caspase complex	5	11
GO:0032991	protein-containing complex	1	11
GO:0042765	GPI-anchor transamidase complex	3	11
GO:0098796	membrane protein complex	2	11
GO:0140534	endoplasmic reticulum protein-containing complex	2	11
GO:1902494	catalytic complex	2	11
GO:1905368	peptidase complex	3	11
GO:1905369	endopeptidase complex	4	11
GO:0005635	nuclear envelope	4	1
GO:0005783	endoplasmic reticulum	5	1
GO:0031967	organelle envelope	3	1
GO:0031975	envelope	2	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4H9E0

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H9E0

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0008152	metabolic process	1	11
GO:0016255	attachment of GPI anchor to protein	5	11
GO:0019538	protein metabolic process	3	11
GO:0036211	protein modification process	4	11
GO:0043170	macromolecule metabolic process	3	11
GO:0043412	macromolecule modification	4	11
GO:0044238	primary metabolic process	2	11
GO:0071704	organic substance metabolic process	2	11
GO:1901564	organonitrogen compound metabolic process	3	11
GO:0008104	protein localization	4	1
GO:0009987	cellular process	1	1
GO:0033036	macromolecule localization	2	1
GO:0034394	protein localization to cell surface	5	1
GO:0051179	localization	1	1
GO:0051641	cellular localization	2	1
GO:0070727	cellular macromolecule localization	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	11
GO:0003923	GPI-anchor transamidase activity	6	11
GO:0004175	endopeptidase activity	4	11
GO:0004197	cysteine-type endopeptidase activity	5	11
GO:0008233	peptidase activity	3	11
GO:0008234	cysteine-type peptidase activity	4	11
GO:0016787	hydrolase activity	2	11
GO:0140096	catalytic activity, acting on a protein	2	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	147	149	PF00675	0.469
CLV_NRD_NRD_1	165	167	PF00675	0.397
CLV_NRD_NRD_1	212	214	PF00675	0.459
CLV_NRD_NRD_1	256	258	PF00675	0.380
CLV_NRD_NRD_1	286	288	PF00675	0.632
CLV_PCSK_KEX2_1	147	149	PF00082	0.469
CLV_PCSK_KEX2_1	165	167	PF00082	0.397
CLV_PCSK_KEX2_1	212	214	PF00082	0.471
CLV_PCSK_KEX2_1	286	288	PF00082	0.609
CLV_PCSK_SKI1_1	147	151	PF00082	0.458
CLV_PCSK_SKI1_1	165	169	PF00082	0.464
CLV_PCSK_SKI1_1	216	220	PF00082	0.455
CLV_PCSK_SKI1_1	64	68	PF00082	0.471
DEG_APCC_DBOX_1	146	154	PF00400	0.271
DOC_CYCLIN_RxL_1	162	171	PF00134	0.337
DOC_MAPK_gen_1	212	220	PF00069	0.258
DOC_MAPK_MEF2A_6	11	20	PF00069	0.325
DOC_MAPK_MEF2A_6	254	263	PF00069	0.180
DOC_PP1_RVXF_1	214	220	PF00149	0.273
DOC_PP1_RVXF_1	9	16	PF00149	0.203
DOC_PP2B_LxvP_1	259	262	PF13499	0.281
DOC_USP7_MATH_1	21	25	PF00917	0.447
DOC_USP7_MATH_1	262	266	PF00917	0.180
DOC_USP7_MATH_1	39	43	PF00917	0.471
DOC_USP7_UBL2_3	185	189	PF12436	0.337
DOC_USP7_UBL2_3	254	258	PF12436	0.180
DOC_WW_Pin1_4	1	6	PF00397	0.549
DOC_WW_Pin1_4	113	118	PF00397	0.180
DOC_WW_Pin1_4	158	163	PF00397	0.271
LIG_14-3-3_CanoR_1	147	153	PF00244	0.331
LIG_14-3-3_CanoR_1	165	175	PF00244	0.178
LIG_14-3-3_CanoR_1	70	78	PF00244	0.256
LIG_BIR_II_1	1	5	PF00653	0.609
LIG_BRCT_BRCA1_1	96	100	PF00533	0.337
LIG_deltaCOP1_diTrp_1	333	339	PF00928	0.342
LIG_EH1_1	6	14	PF00400	0.380
LIG_eIF4E_1	269	275	PF01652	0.197
LIG_eIF4E_1	7	13	PF01652	0.380
LIG_FHA_1	162	168	PF00498	0.333
LIG_FHA_1	283	289	PF00498	0.422
LIG_FHA_1	38	44	PF00498	0.400
LIG_FHA_1	8	14	PF00498	0.553
LIG_LIR_Gen_1	77	87	PF02991	0.337
LIG_LIR_Nem_3	186	190	PF02991	0.265
LIG_LIR_Nem_3	264	269	PF02991	0.281
LIG_LIR_Nem_3	270	275	PF02991	0.297
LIG_LIR_Nem_3	299	303	PF02991	0.277
LIG_LIR_Nem_3	77	82	PF02991	0.300
LIG_SH2_CRK	214	218	PF00017	0.271
LIG_SH2_CRK	79	83	PF00017	0.337
LIG_SH2_STAP1	251	255	PF00017	0.288
LIG_SH2_STAP1	269	273	PF00017	0.308
LIG_SH2_STAP1	30	34	PF00017	0.499
LIG_SH2_STAP1	301	305	PF00017	0.232
LIG_SH2_STAT5	178	181	PF00017	0.290
LIG_SH2_STAT5	187	190	PF00017	0.295
LIG_SH2_STAT5	65	68	PF00017	0.258
LIG_SH3_3	230	236	PF00018	0.361
LIG_SH3_3	322	328	PF00018	0.329
LIG_Sin3_3	15	22	PF02671	0.270
LIG_SUMO_SIM_anti_2	291	297	PF11976	0.373
MOD_CDK_SPK_2	1	6	PF00069	0.549
MOD_CDK_SPxxK_3	158	165	PF00069	0.271
MOD_CK1_1	199	205	PF00069	0.361
MOD_CK1_1	24	30	PF00069	0.674
MOD_CK1_1	299	305	PF00069	0.206
MOD_CK2_1	241	247	PF00069	0.311
MOD_GlcNHglycan	133	136	PF01048	0.513
MOD_GlcNHglycan	311	315	PF01048	0.561
MOD_GSK3_1	166	173	PF00069	0.263
MOD_GSK3_1	190	197	PF00069	0.272
MOD_GSK3_1	20	27	PF00069	0.397
MOD_GSK3_1	241	248	PF00069	0.312
MOD_GSK3_1	278	285	PF00069	0.239
MOD_GSK3_1	310	317	PF00069	0.453
MOD_N-GLC_1	45	50	PF02516	0.591
MOD_N-GLC_1	7	12	PF02516	0.180
MOD_NEK2_1	20	25	PF00069	0.432
MOD_NEK2_1	220	225	PF00069	0.259
MOD_NEK2_1	275	280	PF00069	0.349
MOD_NEK2_1	296	301	PF00069	0.288
MOD_NEK2_1	45	50	PF00069	0.450
MOD_NEK2_1	60	65	PF00069	0.193
MOD_NEK2_1	96	101	PF00069	0.286
MOD_PK_1	148	154	PF00069	0.337
MOD_PKA_2	69	75	PF00069	0.269
MOD_Plk_1	7	13	PF00069	0.380
MOD_Plk_4	148	154	PF00069	0.313
MOD_Plk_4	183	189	PF00069	0.287
MOD_Plk_4	201	207	PF00069	0.168
MOD_Plk_4	262	268	PF00069	0.303
MOD_Plk_4	291	297	PF00069	0.347
MOD_Plk_4	74	80	PF00069	0.271
MOD_ProDKin_1	1	7	PF00069	0.549
MOD_ProDKin_1	113	119	PF00069	0.180
MOD_ProDKin_1	158	164	PF00069	0.271
MOD_SUMO_rev_2	250	259	PF00179	0.180
TRG_DiLeu_BaLyEn_6	163	168	PF01217	0.337
TRG_DiLeu_BaLyEn_6	8	13	PF01217	0.380
TRG_ENDOCYTIC_2	187	190	PF00928	0.353
TRG_ENDOCYTIC_2	214	217	PF00928	0.271
TRG_ENDOCYTIC_2	272	275	PF00928	0.315
TRG_ENDOCYTIC_2	79	82	PF00928	0.337
TRG_ER_diArg_1	125	128	PF00400	0.317
TRG_ER_diArg_1	146	148	PF00400	0.270
TRG_ER_diArg_1	165	167	PF00400	0.173
TRG_ER_diArg_1	212	214	PF00400	0.271
TRG_ER_diArg_1	286	288	PF00400	0.432
TRG_ER_diArg_1	82	85	PF00400	0.180
TRG_Pf-PMV_PEXEL_1	165	170	PF00026	0.458
TRG_Pf-PMV_PEXEL_1	85	89	PF00026	0.380

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IJ31	Leptomonas seymouri	73%	98%
A0A1X0P6S3	Trypanosomatidae	53%	100%
A0A3S5H727	Leishmania donovani	83%	100%
A4HXR1	Leishmania infantum	82%	100%
B6DTF5	Bodo saltans	41%	100%
D0A043	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	54%	100%
E9ARH3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	83%	100%
O89017	Mus musculus	25%	82%
P49044	Vicia sativa	25%	72%
P49047	Arabidopsis thaliana	27%	75%
Q3MHZ7	Bos taurus	39%	90%
Q4KRV1	Sus scrofa	37%	90%
Q4QE06	Leishmania major	80%	100%
Q4R4T8	Macaca fascicularis	26%	82%
Q5R5D9	Pongo abelii	27%	82%
Q5R6L8	Pongo abelii	39%	90%
Q8T4E1	Drosophila melanogaster	33%	100%
Q92643	Homo sapiens	39%	90%
Q95M12	Bos taurus	26%	82%
Q99538	Homo sapiens	27%	82%
Q9CXY9	Mus musculus	39%	90%
Q9R0J8	Rattus norvegicus	26%	82%
V5BWA3	Trypanosoma cruzi	52%	100%