3-oxo-5-alpha-steroid 4-dehydrogenase-like protein

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

3-oxo-5-alpha-steroid 4-dehydrogenase-like protein

Species:

Leishmania braziliensis

UniProt:

A4H965_LEIBR

TriTrypDb:

LbrM.17.1210 , LBRM2903_170019000 , LBRM2903_170019100

Length:

539

Annotations

LeishMANIAdb annotations

Related to other eukaryotic steroid 5 alpha reductases. Localization: ER (by homology)

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 6
NetGPI	no	yes: 0, no: 6

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	7
GO:0110165	cellular anatomical entity	1	7

Expansion

Sequence features

A4H965

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H965

Function

Biological processes
Term	Name	Level	Count
GO:0006629	lipid metabolic process	3	7
GO:0008152	metabolic process	1	7
GO:0044238	primary metabolic process	2	7
GO:0071704	organic substance metabolic process	2	7

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	7
GO:0003865	3-oxo-5-alpha-steroid 4-dehydrogenase activity	5	6
GO:0016229	steroid dehydrogenase activity	3	6
GO:0016491	oxidoreductase activity	2	7
GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors	3	7
GO:0033765	steroid dehydrogenase activity, acting on the CH-CH group of donors	4	6

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	85	89	PF00656	0.681
CLV_NRD_NRD_1	298	300	PF00675	0.298
CLV_NRD_NRD_1	359	361	PF00675	0.593
CLV_NRD_NRD_1	530	532	PF00675	0.278
CLV_PCSK_KEX2_1	300	302	PF00082	0.293
CLV_PCSK_KEX2_1	358	360	PF00082	0.594
CLV_PCSK_PC1ET2_1	300	302	PF00082	0.293
CLV_PCSK_PC7_1	296	302	PF00082	0.290
CLV_PCSK_SKI1_1	531	535	PF00082	0.346
CLV_Separin_Metazoa	456	460	PF03568	0.387
DEG_SCF_FBW7_1	286	293	PF00400	0.580
DEG_SPOP_SBC_1	386	390	PF00917	0.603
DEG_SPOP_SBC_1	460	464	PF00917	0.463
DOC_CYCLIN_yCln2_LP_2	233	239	PF00134	0.471
DOC_MAPK_MEF2A_6	125	134	PF00069	0.331
DOC_PP1_RVXF_1	137	143	PF00149	0.424
DOC_PP2B_LxvP_1	134	137	PF13499	0.487
DOC_PP2B_LxvP_1	233	236	PF13499	0.383
DOC_SPAK_OSR1_1	116	120	PF12202	0.512
DOC_USP7_MATH_1	290	294	PF00917	0.674
DOC_USP7_MATH_1	44	48	PF00917	0.244
DOC_USP7_MATH_1	460	464	PF00917	0.463
DOC_WW_Pin1_4	144	149	PF00397	0.367
DOC_WW_Pin1_4	178	183	PF00397	0.416
DOC_WW_Pin1_4	286	291	PF00397	0.693
LIG_14-3-3_CanoR_1	116	122	PF00244	0.411
LIG_14-3-3_CanoR_1	133	137	PF00244	0.336
LIG_14-3-3_CanoR_1	139	143	PF00244	0.327
LIG_14-3-3_CanoR_1	459	469	PF00244	0.411
LIG_14-3-3_CanoR_1	99	103	PF00244	0.444
LIG_Actin_WH2_2	100	118	PF00022	0.512
LIG_BRCT_BRCA1_1	290	294	PF00533	0.598
LIG_BRCT_BRCA1_1	336	340	PF00533	0.512
LIG_BRCT_BRCA1_1	409	413	PF00533	0.227
LIG_BRCT_BRCA1_1	46	50	PF00533	0.244
LIG_BRCT_BRCA1_1	99	103	PF00533	0.524
LIG_deltaCOP1_diTrp_1	58	65	PF00928	0.748
LIG_FHA_1	104	110	PF00498	0.431
LIG_FHA_1	16	22	PF00498	0.411
LIG_FHA_1	328	334	PF00498	0.244
LIG_FHA_1	427	433	PF00498	0.385
LIG_FHA_1	5	11	PF00498	0.448
LIG_FHA_2	449	455	PF00498	0.471
LIG_FHA_2	519	525	PF00498	0.618
LIG_GBD_Chelix_1	449	457	PF00786	0.506
LIG_Integrin_isoDGR_2	257	259	PF01839	0.361
LIG_LIR_Apic_2	228	232	PF02991	0.371
LIG_LIR_Gen_1	111	119	PF02991	0.461
LIG_LIR_Gen_1	18	26	PF02991	0.478
LIG_LIR_Gen_1	251	260	PF02991	0.440
LIG_LIR_Gen_1	410	421	PF02991	0.227
LIG_LIR_Gen_1	429	437	PF02991	0.418
LIG_LIR_Gen_1	464	474	PF02991	0.487
LIG_LIR_Gen_1	524	534	PF02991	0.618
LIG_LIR_Nem_3	111	115	PF02991	0.461
LIG_LIR_Nem_3	160	164	PF02991	0.366
LIG_LIR_Nem_3	18	22	PF02991	0.478
LIG_LIR_Nem_3	201	206	PF02991	0.409
LIG_LIR_Nem_3	251	256	PF02991	0.411
LIG_LIR_Nem_3	351	355	PF02991	0.384
LIG_LIR_Nem_3	410	416	PF02991	0.227
LIG_LIR_Nem_3	427	431	PF02991	0.346
LIG_LIR_Nem_3	463	469	PF02991	0.499
LIG_LIR_Nem_3	498	502	PF02991	0.411
LIG_LIR_Nem_3	524	529	PF02991	0.556
LIG_LYPXL_yS_3	161	164	PF13949	0.486
LIG_NRBOX	214	220	PF00104	0.447
LIG_PCNA_PIPBox_1	249	258	PF02747	0.471
LIG_Pex14_1	323	327	PF04695	0.284
LIG_Pex14_2	294	298	PF04695	0.592
LIG_SH2_CRK	155	159	PF00017	0.278
LIG_SH2_CRK	405	409	PF00017	0.346
LIG_SH2_CRK	526	530	PF00017	0.618
LIG_SH2_PTP2	112	115	PF00017	0.244
LIG_SH2_PTP2	431	434	PF00017	0.346
LIG_SH2_SRC	353	356	PF00017	0.274
LIG_SH2_SRC	431	434	PF00017	0.418
LIG_SH2_STAP1	33	37	PF00017	0.332
LIG_SH2_STAP1	518	522	PF00017	0.618
LIG_SH2_STAT3	73	76	PF00017	0.728
LIG_SH2_STAT5	112	115	PF00017	0.244
LIG_SH2_STAT5	224	227	PF00017	0.365
LIG_SH2_STAT5	24	27	PF00017	0.411
LIG_SH2_STAT5	253	256	PF00017	0.411
LIG_SH2_STAT5	288	291	PF00017	0.695
LIG_SH2_STAT5	329	332	PF00017	0.312
LIG_SH2_STAT5	35	38	PF00017	0.437
LIG_SH2_STAT5	353	356	PF00017	0.394
LIG_SH2_STAT5	431	434	PF00017	0.346
LIG_SH2_STAT5	502	505	PF00017	0.411
LIG_SH2_STAT5	526	529	PF00017	0.618
LIG_SH3_3	116	122	PF00018	0.364
LIG_SH3_4	371	378	PF00018	0.417
LIG_SUMO_SIM_anti_2	106	111	PF11976	0.244
LIG_SUMO_SIM_par_1	105	111	PF11976	0.512
LIG_SUMO_SIM_par_1	242	247	PF11976	0.412
LIG_SUMO_SIM_par_1	431	436	PF11976	0.255
LIG_TRAF2_1	68	71	PF00917	0.752
LIG_TYR_ITIM	110	115	PF00017	0.286
LIG_TYR_ITIM	153	158	PF00017	0.481
LIG_TYR_ITIM	159	164	PF00017	0.579
LIG_TYR_ITSM	427	434	PF00017	0.512
MOD_CK1_1	138	144	PF00069	0.469
MOD_CK1_1	170	176	PF00069	0.649
MOD_CK1_1	178	184	PF00069	0.620
MOD_CK1_1	195	201	PF00069	0.593
MOD_CK1_1	306	312	PF00069	0.572
MOD_CK1_1	376	382	PF00069	0.572
MOD_CK1_1	387	393	PF00069	0.613
MOD_CK1_1	436	442	PF00069	0.472
MOD_CK1_1	445	451	PF00069	0.517
MOD_CK1_1	462	468	PF00069	0.404
MOD_CK2_1	448	454	PF00069	0.550
MOD_CK2_1	482	488	PF00069	0.508
MOD_CK2_1	65	71	PF00069	0.434
MOD_CMANNOS	59	62	PF00535	0.667
MOD_Cter_Amidation	529	532	PF01082	0.286
MOD_GlcNHglycan	237	240	PF01048	0.378
MOD_GlcNHglycan	264	267	PF01048	0.581
MOD_GlcNHglycan	27	30	PF01048	0.638
MOD_GlcNHglycan	281	284	PF01048	0.629
MOD_GlcNHglycan	308	311	PF01048	0.536
MOD_GlcNHglycan	336	339	PF01048	0.471
MOD_GlcNHglycan	375	378	PF01048	0.520
MOD_GlcNHglycan	389	392	PF01048	0.504
MOD_GlcNHglycan	409	412	PF01048	0.244
MOD_GlcNHglycan	435	438	PF01048	0.479
MOD_GlcNHglycan	46	49	PF01048	0.199
MOD_GlcNHglycan	484	487	PF01048	0.528
MOD_GlcNHglycan	84	87	PF01048	0.566
MOD_GSK3_1	169	176	PF00069	0.679
MOD_GSK3_1	191	198	PF00069	0.662
MOD_GSK3_1	231	238	PF00069	0.486
MOD_GSK3_1	244	251	PF00069	0.382
MOD_GSK3_1	272	279	PF00069	0.680
MOD_GSK3_1	286	293	PF00069	0.470
MOD_GSK3_1	304	311	PF00069	0.513
MOD_GSK3_1	363	370	PF00069	0.587
MOD_GSK3_1	386	393	PF00069	0.754
MOD_GSK3_1	435	442	PF00069	0.443
MOD_GSK3_1	461	468	PF00069	0.647
MOD_GSK3_1	482	489	PF00069	0.639
MOD_GSK3_1	84	91	PF00069	0.382
MOD_N-GLC_1	363	368	PF02516	0.453
MOD_N-GLC_1	39	44	PF02516	0.244
MOD_N-GLC_1	442	447	PF02516	0.541
MOD_NEK2_1	103	108	PF00069	0.454
MOD_NEK2_1	117	122	PF00069	0.604
MOD_NEK2_1	132	137	PF00069	0.618
MOD_NEK2_1	15	20	PF00069	0.512
MOD_NEK2_1	169	174	PF00069	0.473
MOD_NEK2_1	177	182	PF00069	0.485
MOD_NEK2_1	218	223	PF00069	0.281
MOD_NEK2_1	244	249	PF00069	0.404
MOD_NEK2_1	304	309	PF00069	0.494
MOD_NEK2_1	327	332	PF00069	0.419
MOD_NEK2_1	334	339	PF00069	0.482
MOD_NEK2_1	385	390	PF00069	0.733
MOD_NEK2_1	461	466	PF00069	0.534
MOD_NEK2_1	65	70	PF00069	0.670
MOD_NEK2_2	248	253	PF00069	0.411
MOD_PIKK_1	208	214	PF00454	0.486
MOD_PK_1	192	198	PF00069	0.453
MOD_PKA_2	132	138	PF00069	0.516
MOD_PKA_2	191	197	PF00069	0.469
MOD_PKA_2	524	530	PF00069	0.300
MOD_PKA_2	98	104	PF00069	0.289
MOD_Plk_1	192	198	PF00069	0.453
MOD_Plk_2-3	363	369	PF00069	0.460
MOD_Plk_4	138	144	PF00069	0.469
MOD_Plk_4	15	21	PF00069	0.512
MOD_Plk_4	198	204	PF00069	0.525
MOD_Plk_4	244	250	PF00069	0.439
MOD_Plk_4	290	296	PF00069	0.366
MOD_Plk_4	46	52	PF00069	0.285
MOD_Plk_4	462	468	PF00069	0.597
MOD_Plk_4	98	104	PF00069	0.342
MOD_ProDKin_1	144	150	PF00069	0.439
MOD_ProDKin_1	178	184	PF00069	0.514
MOD_ProDKin_1	286	292	PF00069	0.615
TRG_ENDOCYTIC_2	112	115	PF00928	0.512
TRG_ENDOCYTIC_2	155	158	PF00928	0.577
TRG_ENDOCYTIC_2	161	164	PF00928	0.492
TRG_ENDOCYTIC_2	253	256	PF00928	0.411
TRG_ENDOCYTIC_2	352	355	PF00928	0.473
TRG_ENDOCYTIC_2	431	434	PF00928	0.411
TRG_ENDOCYTIC_2	526	529	PF00928	0.411
TRG_ER_diArg_1	298	301	PF00400	0.332
TRG_ER_diArg_1	357	360	PF00400	0.460

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P837	Leptomonas seymouri	46%	100%
A0A3S7WUK3	Leishmania donovani	72%	100%
A4HXI7	Leishmania infantum	72%	100%
E9AR82	Leishmania mexicana (strain MHOM/GT/2001/U1103)	72%	100%
Q4QE81	Leishmania major	72%	100%