Splicing factor 3A

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Splicing factor 3A

Gene product:

splicing factor 3A

Species:

Leishmania braziliensis

UniProt:

A4H930_LEIBR

TriTrypDb:

LbrM.17.0860 , LBRM2903_170015100

Length:

619

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005681	spliceosomal complex	3	11
GO:0032991	protein-containing complex	1	11
GO:0140513	nuclear protein-containing complex	2	11
GO:1990904	ribonucleoprotein complex	2	11

Expansion

Sequence features

A4H930

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H930

Function

Biological processes
Term	Name	Level	Count
GO:0000375	RNA splicing, via transesterification reactions	8	11
GO:0000377	RNA splicing, via transesterification reactions with bulged adenosine as nucleophile	9	11
GO:0000398	mRNA splicing, via spliceosome	8	11
GO:0006139	nucleobase-containing compound metabolic process	3	11
GO:0006396	RNA processing	6	11
GO:0006397	mRNA processing	7	11
GO:0006725	cellular aromatic compound metabolic process	3	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0008152	metabolic process	1	11
GO:0008380	RNA splicing	7	11
GO:0009987	cellular process	1	11
GO:0016070	RNA metabolic process	5	11
GO:0016071	mRNA metabolic process	6	11
GO:0034641	cellular nitrogen compound metabolic process	3	11
GO:0043170	macromolecule metabolic process	3	11
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0046483	heterocycle metabolic process	3	11
GO:0071704	organic substance metabolic process	2	11
GO:0090304	nucleic acid metabolic process	4	11
GO:1901360	organic cyclic compound metabolic process	3	11

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	11
GO:0003723	RNA binding	4	11
GO:0005488	binding	1	11
GO:0008270	zinc ion binding	6	10
GO:0043167	ion binding	2	11
GO:0043169	cation binding	3	11
GO:0046872	metal ion binding	4	11
GO:0046914	transition metal ion binding	5	10
GO:0097159	organic cyclic compound binding	2	11
GO:1901363	heterocyclic compound binding	2	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	285	289	PF00656	0.526
CLV_C14_Caspase3-7	591	595	PF00656	0.319
CLV_C14_Caspase3-7	597	601	PF00656	0.276
CLV_NRD_NRD_1	210	212	PF00675	0.324
CLV_NRD_NRD_1	235	237	PF00675	0.537
CLV_NRD_NRD_1	551	553	PF00675	0.318
CLV_NRD_NRD_1	585	587	PF00675	0.281
CLV_PCSK_KEX2_1	235	237	PF00082	0.472
CLV_PCSK_KEX2_1	551	553	PF00082	0.318
CLV_PCSK_KEX2_1	587	589	PF00082	0.281
CLV_PCSK_KEX2_1	617	619	PF00082	0.569
CLV_PCSK_PC1ET2_1	587	589	PF00082	0.281
CLV_PCSK_PC7_1	613	619	PF00082	0.429
CLV_PCSK_SKI1_1	247	251	PF00082	0.331
CLV_PCSK_SKI1_1	39	43	PF00082	0.427
CLV_PCSK_SKI1_1	406	410	PF00082	0.384
CLV_PCSK_SKI1_1	458	462	PF00082	0.603
DEG_Nend_UBRbox_3	1	3	PF02207	0.633
DEG_SPOP_SBC_1	303	307	PF00917	0.777
DOC_CYCLIN_RxL_1	34	46	PF00134	0.466
DOC_CYCLIN_RxL_1	403	413	PF00134	0.382
DOC_CYCLIN_yClb1_LxF_4	187	192	PF00134	0.503
DOC_MAPK_DCC_7	235	245	PF00069	0.286
DOC_MAPK_DCC_7	338	347	PF00069	0.494
DOC_MAPK_gen_1	34	42	PF00069	0.264
DOC_MAPK_MEF2A_6	197	204	PF00069	0.468
DOC_PP1_RVXF_1	187	193	PF00149	0.501
DOC_PP1_RVXF_1	210	217	PF00149	0.456
DOC_PP2B_LxvP_1	115	118	PF13499	0.501
DOC_PP2B_LxvP_1	327	330	PF13499	0.396
DOC_USP7_MATH_1	161	165	PF00917	0.594
DOC_USP7_MATH_1	275	279	PF00917	0.658
DOC_USP7_MATH_1	303	307	PF00917	0.799
DOC_USP7_MATH_1	367	371	PF00917	0.625
DOC_WW_Pin1_4	77	82	PF00397	0.549
LIG_14-3-3_CanoR_1	145	152	PF00244	0.466
LIG_14-3-3_CanoR_1	235	239	PF00244	0.260
LIG_14-3-3_CanoR_1	438	445	PF00244	0.448
LIG_14-3-3_CanoR_1	54	61	PF00244	0.438
LIG_14-3-3_CanoR_1	552	562	PF00244	0.229
LIG_14-3-3_CanoR_1	577	585	PF00244	0.199
LIG_Actin_WH2_2	442	460	PF00022	0.492
LIG_APCC_ABBA_1	61	66	PF00400	0.405
LIG_BIR_III_4	288	292	PF00653	0.537
LIG_BIR_III_4	600	604	PF00653	0.300
LIG_CtBP_PxDLS_1	330	334	PF00389	0.478
LIG_eIF4E_1	55	61	PF01652	0.272
LIG_FHA_1	1	7	PF00498	0.528
LIG_FHA_1	151	157	PF00498	0.493
LIG_FHA_1	194	200	PF00498	0.469
LIG_FHA_1	235	241	PF00498	0.422
LIG_FHA_1	473	479	PF00498	0.678
LIG_FHA_1	554	560	PF00498	0.193
LIG_FHA_2	102	108	PF00498	0.346
LIG_FHA_2	126	132	PF00498	0.412
LIG_FHA_2	226	232	PF00498	0.243
LIG_FHA_2	248	254	PF00498	0.341
LIG_FHA_2	397	403	PF00498	0.400
LIG_FHA_2	413	419	PF00498	0.345
LIG_FHA_2	484	490	PF00498	0.596
LIG_FHA_2	577	583	PF00498	0.342
LIG_FHA_2	591	597	PF00498	0.362
LIG_FHA_2	605	611	PF00498	0.213
LIG_KLC1_Yacidic_2	62	66	PF13176	0.405
LIG_LIR_Apic_2	341	346	PF02991	0.426
LIG_LIR_Gen_1	257	268	PF02991	0.383
LIG_LIR_Gen_1	395	402	PF02991	0.364
LIG_LIR_Nem_3	104	108	PF02991	0.413
LIG_LIR_Nem_3	140	146	PF02991	0.334
LIG_LIR_Nem_3	147	152	PF02991	0.340
LIG_LIR_Nem_3	191	195	PF02991	0.367
LIG_LIR_Nem_3	257	263	PF02991	0.372
LIG_LIR_Nem_3	395	401	PF02991	0.363
LIG_LIR_Nem_3	561	565	PF02991	0.300
LIG_LIR_Nem_3	610	614	PF02991	0.362
LIG_NRBOX	392	398	PF00104	0.326
LIG_NRP_CendR_1	617	619	PF00754	0.638
LIG_Pex14_2	537	541	PF04695	0.281
LIG_PTAP_UEV_1	484	489	PF05743	0.495
LIG_RPA_C_Fungi	206	218	PF08784	0.391
LIG_SH2_CRK	531	535	PF00017	0.413
LIG_SH2_STAP1	146	150	PF00017	0.321
LIG_SH2_STAP1	152	156	PF00017	0.361
LIG_SH2_STAP1	412	416	PF00017	0.440
LIG_SH2_STAT5	152	155	PF00017	0.281
LIG_SH2_STAT5	229	232	PF00017	0.328
LIG_SH2_STAT5	398	401	PF00017	0.446
LIG_SH2_STAT5	55	58	PF00017	0.370
LIG_SH2_STAT5	611	614	PF00017	0.474
LIG_SH2_STAT5	64	67	PF00017	0.428
LIG_SH3_1	236	242	PF00018	0.441
LIG_SH3_3	114	120	PF00018	0.465
LIG_SH3_3	170	176	PF00018	0.580
LIG_SH3_3	236	242	PF00018	0.441
LIG_SH3_3	477	483	PF00018	0.727
LIG_SUMO_SIM_par_1	241	246	PF11976	0.422
LIG_TRAF2_1	128	131	PF00917	0.280
LIG_TRAF2_1	415	418	PF00917	0.454
LIG_TRAF2_1	426	429	PF00917	0.421
LIG_TRFH_1	340	344	PF08558	0.337
LIG_TYR_ITIM	396	401	PF00017	0.377
LIG_WRC_WIRS_1	192	197	PF05994	0.422
LIG_WRC_WIRS_1	219	224	PF05994	0.238
MOD_CK1_1	164	170	PF00069	0.660
MOD_CK1_1	359	365	PF00069	0.684
MOD_CK1_1	441	447	PF00069	0.482
MOD_CK1_1	464	470	PF00069	0.674
MOD_CK2_1	125	131	PF00069	0.368
MOD_CK2_1	152	158	PF00069	0.471
MOD_CK2_1	225	231	PF00069	0.381
MOD_CK2_1	247	253	PF00069	0.401
MOD_CK2_1	259	265	PF00069	0.430
MOD_CK2_1	396	402	PF00069	0.398
MOD_CK2_1	412	418	PF00069	0.353
MOD_CK2_1	430	436	PF00069	0.398
MOD_CK2_1	483	489	PF00069	0.733
MOD_CK2_1	576	582	PF00069	0.475
MOD_CK2_1	590	596	PF00069	0.362
MOD_CK2_1	604	610	PF00069	0.213
MOD_GlcNHglycan	146	149	PF01048	0.467
MOD_GlcNHglycan	161	164	PF01048	0.633
MOD_GlcNHglycan	367	370	PF01048	0.670
MOD_GlcNHglycan	440	443	PF01048	0.460
MOD_GlcNHglycan	466	469	PF01048	0.627
MOD_GlcNHglycan	475	478	PF01048	0.731
MOD_GSK3_1	247	254	PF00069	0.295
MOD_GSK3_1	259	266	PF00069	0.487
MOD_GSK3_1	299	306	PF00069	0.807
MOD_GSK3_1	325	332	PF00069	0.394
MOD_GSK3_1	358	365	PF00069	0.575
MOD_GSK3_1	392	399	PF00069	0.428
MOD_GSK3_1	400	407	PF00069	0.375
MOD_GSK3_1	441	448	PF00069	0.419
MOD_N-GLC_1	404	409	PF02516	0.461
MOD_NEK2_1	150	155	PF00069	0.360
MOD_NEK2_1	180	185	PF00069	0.480
MOD_NEK2_1	193	198	PF00069	0.473
MOD_NEK2_1	218	223	PF00069	0.241
MOD_NEK2_1	263	268	PF00069	0.502
MOD_NEK2_1	29	34	PF00069	0.420
MOD_NEK2_1	392	397	PF00069	0.347
MOD_NEK2_1	401	406	PF00069	0.364
MOD_NEK2_1	430	435	PF00069	0.567
MOD_NEK2_1	445	450	PF00069	0.552
MOD_NEK2_1	461	466	PF00069	0.622
MOD_NEK2_1	472	477	PF00069	0.561
MOD_NEK2_1	553	558	PF00069	0.193
MOD_NEK2_2	367	372	PF00069	0.389
MOD_NEK2_2	564	569	PF00069	0.281
MOD_NEK2_2	581	586	PF00069	0.199
MOD_PKA_1	338	344	PF00069	0.424
MOD_PKA_2	144	150	PF00069	0.350
MOD_PKA_2	234	240	PF00069	0.262
MOD_PKA_2	53	59	PF00069	0.434
MOD_PKA_2	576	582	PF00069	0.274
MOD_PKB_1	588	596	PF00069	0.468
MOD_Plk_1	268	274	PF00069	0.557
MOD_Plk_1	404	410	PF00069	0.516
MOD_Plk_2-3	125	131	PF00069	0.493
MOD_Plk_2-3	225	231	PF00069	0.241
MOD_Plk_2-3	251	257	PF00069	0.447
MOD_Plk_2-3	576	582	PF00069	0.199
MOD_Plk_4	175	181	PF00069	0.544
MOD_Plk_4	19	25	PF00069	0.423
MOD_Plk_4	225	231	PF00069	0.346
MOD_Plk_4	259	265	PF00069	0.532
MOD_Plk_4	367	373	PF00069	0.537
MOD_Plk_4	392	398	PF00069	0.378
MOD_Plk_4	404	410	PF00069	0.364
MOD_ProDKin_1	77	83	PF00069	0.544
MOD_SUMO_for_1	33	36	PF00179	0.292
MOD_SUMO_rev_2	262	268	PF00179	0.434
MOD_SUMO_rev_2	375	382	PF00179	0.383
MOD_SUMO_rev_2	90	96	PF00179	0.521
TRG_DiLeu_BaEn_1	495	500	PF01217	0.648
TRG_DiLeu_BaLyEn_6	118	123	PF01217	0.452
TRG_DiLeu_BaLyEn_6	343	348	PF01217	0.434
TRG_ENDOCYTIC_2	105	108	PF00928	0.553
TRG_ENDOCYTIC_2	146	149	PF00928	0.323
TRG_ENDOCYTIC_2	219	222	PF00928	0.337
TRG_ENDOCYTIC_2	398	401	PF00928	0.446
TRG_ENDOCYTIC_2	562	565	PF00928	0.300
TRG_ENDOCYTIC_2	611	614	PF00928	0.300
TRG_ER_diArg_1	234	236	PF00400	0.420
TRG_ER_diArg_1	37	40	PF00400	0.460
TRG_ER_diArg_1	550	552	PF00400	0.318
TRG_ER_diArg_1	585	588	PF00400	0.281
TRG_ER_diArg_1	617	619	PF00400	0.669
TRG_NES_CRM1_1	563	576	PF08389	0.371
TRG_NLS_MonoExtC_3	585	590	PF00514	0.300
TRG_Pf-PMV_PEXEL_1	10	15	PF00026	0.497
TRG_Pf-PMV_PEXEL_1	121	125	PF00026	0.464
TRG_Pf-PMV_PEXEL_1	154	158	PF00026	0.458
TRG_Pf-PMV_PEXEL_1	39	43	PF00026	0.437
TRG_Pf-PMV_PEXEL_1	414	418	PF00026	0.550
TRG_Pf-PMV_PEXEL_1	509	513	PF00026	0.360
TRG_PTS2	1	45	PF00400	0.390

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P5M4	Leptomonas seymouri	62%	97%
A0A1X0P1R3	Trypanosomatidae	42%	100%
A0A3S7X3F0	Leishmania donovani	84%	100%
A0A422P3I2	Trypanosoma rangeli	44%	100%
A4I5K9	Leishmania infantum	84%	100%
C9ZQY7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	42%	100%
E9B0V4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	84%	100%
Q4Q7B2	Leishmania major	84%	100%
V5DQL3	Trypanosoma cruzi	43%	100%