VASt domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

GRAM domain containing protein, putative

Species:

Leishmania braziliensis

UniProt:

A4H910_LEIBR

TriTrypDb:

LbrM.17.0660 , LBRM2903_170013000

Length:

361

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	11
GO:0110165	cellular anatomical entity	1	11
GO:0005789	endoplasmic reticulum membrane	4	1
GO:0005886	plasma membrane	3	1
GO:0031090	organelle membrane	3	1
GO:0044232	organelle membrane contact site	2	1
GO:0140268	endoplasmic reticulum-plasma membrane contact site	3	1

Expansion

Sequence features

A4H910

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H910

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	1
GO:0006869	lipid transport	5	1
GO:0009987	cellular process	1	1
GO:0015850	organic hydroxy compound transport	5	1
GO:0015918	sterol transport	6	1
GO:0032365	intracellular lipid transport	4	1
GO:0032366	intracellular sterol transport	5	1
GO:0046907	intracellular transport	3	1
GO:0051179	localization	1	1
GO:0051234	establishment of localization	2	1
GO:0051641	cellular localization	2	1
GO:0051649	establishment of localization in cell	3	1
GO:0071702	organic substance transport	4	1

Molecular functions
Term	Name	Level	Count
GO:0005215	transporter activity	1	1
GO:0005319	lipid transporter activity	2	1
GO:0005488	binding	1	1
GO:0005496	steroid binding	3	1
GO:0008289	lipid binding	2	1
GO:0015248	sterol transporter activity	3	1
GO:0032934	sterol binding	4	1
GO:0097159	organic cyclic compound binding	2	1
GO:0120013	lipid transfer activity	3	1
GO:0120015	sterol transfer activity	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	303	307	PF00656	0.442
CLV_MEL_PAP_1	295	301	PF00089	0.520
CLV_NRD_NRD_1	356	358	PF00675	0.498
CLV_NRD_NRD_1	78	80	PF00675	0.238
CLV_PCSK_KEX2_1	356	358	PF00082	0.432
CLV_PCSK_SKI1_1	102	106	PF00082	0.255
CLV_PCSK_SKI1_1	125	129	PF00082	0.262
CLV_PCSK_SKI1_1	324	328	PF00082	0.474
CLV_PCSK_SKI1_1	55	59	PF00082	0.272
DEG_APCC_DBOX_1	124	132	PF00400	0.492
DEG_APCC_DBOX_1	323	331	PF00400	0.202
DEG_Nend_UBRbox_2	1	3	PF02207	0.498
DOC_CKS1_1	45	50	PF01111	0.606
DOC_MAPK_DCC_7	43	51	PF00069	0.502
DOC_MAPK_gen_1	207	214	PF00069	0.441
DOC_MAPK_MEF2A_6	130	139	PF00069	0.498
DOC_MAPK_MEF2A_6	324	332	PF00069	0.202
DOC_USP7_MATH_1	17	21	PF00917	0.629
DOC_USP7_MATH_1	218	222	PF00917	0.610
DOC_USP7_MATH_1	240	244	PF00917	0.668
DOC_USP7_MATH_1	286	290	PF00917	0.315
DOC_WW_Pin1_4	131	136	PF00397	0.546
DOC_WW_Pin1_4	44	49	PF00397	0.617
LIG_14-3-3_CanoR_1	102	110	PF00244	0.450
LIG_14-3-3_CanoR_1	192	202	PF00244	0.504
LIG_14-3-3_CanoR_1	287	291	PF00244	0.455
LIG_14-3-3_CterR_2	356	361	PF00244	0.558
LIG_Actin_WH2_2	170	187	PF00022	0.405
LIG_Actin_WH2_2	263	279	PF00022	0.467
LIG_AP2alpha_1	150	154	PF02296	0.480
LIG_CaM_NSCaTE_8	316	323	PF13499	0.411
LIG_EH1_1	153	161	PF00400	0.424
LIG_eIF4E_1	123	129	PF01652	0.492
LIG_FHA_1	105	111	PF00498	0.451
LIG_FHA_1	132	138	PF00498	0.449
LIG_FHA_1	172	178	PF00498	0.441
LIG_FHA_1	262	268	PF00498	0.441
LIG_FHA_1	280	286	PF00498	0.304
LIG_FHA_1	67	73	PF00498	0.540
LIG_FHA_2	194	200	PF00498	0.457
LIG_FHA_2	4	10	PF00498	0.576
LIG_IRF3_LxIS_1	330	336	PF10401	0.420
LIG_LIR_Gen_1	22	31	PF02991	0.545
LIG_LIR_Gen_1	278	288	PF02991	0.504
LIG_LIR_Gen_1	36	46	PF02991	0.510
LIG_LIR_Nem_3	122	126	PF02991	0.477
LIG_LIR_Nem_3	22	27	PF02991	0.562
LIG_LIR_Nem_3	278	283	PF02991	0.444
LIG_LIR_Nem_3	315	319	PF02991	0.482
LIG_LIR_Nem_3	336	341	PF02991	0.483
LIG_LIR_Nem_3	36	41	PF02991	0.504
LIG_MYND_1	247	251	PF01753	0.671
LIG_Pex14_1	280	284	PF04695	0.287
LIG_Pex14_2	150	154	PF04695	0.480
LIG_Pex14_2	61	65	PF04695	0.462
LIG_REV1ctd_RIR_1	281	291	PF16727	0.297
LIG_SH2_SRC	118	121	PF00017	0.519
LIG_SH2_STAT5	120	123	PF00017	0.536
LIG_SH2_STAT5	180	183	PF00017	0.531
LIG_SH2_STAT5	331	334	PF00017	0.451
LIG_SH3_3	42	48	PF00018	0.589
MOD_CDK_SPxK_1	44	50	PF00069	0.593
MOD_CK1_1	167	173	PF00069	0.530
MOD_CK1_1	33	39	PF00069	0.521
MOD_CK2_1	22	28	PF00069	0.550
MOD_CK2_1	3	9	PF00069	0.650
MOD_CK2_1	36	42	PF00069	0.476
MOD_CMANNOS	86	89	PF00535	0.262
MOD_Cter_Amidation	354	357	PF01082	0.421
MOD_GlcNHglycan	131	134	PF01048	0.336
MOD_GlcNHglycan	166	169	PF01048	0.340
MOD_GlcNHglycan	221	224	PF01048	0.476
MOD_GlcNHglycan	277	280	PF01048	0.564
MOD_GlcNHglycan	32	35	PF01048	0.389
MOD_GlcNHglycan	320	323	PF01048	0.679
MOD_GSK3_1	129	136	PF00069	0.546
MOD_GSK3_1	160	167	PF00069	0.531
MOD_GSK3_1	193	200	PF00069	0.524
MOD_GSK3_1	22	29	PF00069	0.542
MOD_GSK3_1	241	248	PF00069	0.596
MOD_GSK3_1	275	282	PF00069	0.509
MOD_GSK3_1	85	92	PF00069	0.455
MOD_NEK2_1	104	109	PF00069	0.462
MOD_NEK2_1	129	134	PF00069	0.556
MOD_NEK2_1	255	260	PF00069	0.691
MOD_NEK2_1	3	8	PF00069	0.588
MOD_NEK2_1	333	338	PF00069	0.354
MOD_NEK2_2	171	176	PF00069	0.494
MOD_NEK2_2	279	284	PF00069	0.331
MOD_PIKK_1	140	146	PF00454	0.505
MOD_PIKK_1	160	166	PF00454	0.489
MOD_PIKK_1	22	28	PF00454	0.623
MOD_PKA_1	79	85	PF00069	0.448
MOD_PKA_2	286	292	PF00069	0.486
MOD_PKA_2	355	361	PF00069	0.617
MOD_Plk_1	171	177	PF00069	0.511
MOD_Plk_1	240	246	PF00069	0.661
MOD_Plk_1	36	42	PF00069	0.533
MOD_Plk_1	66	72	PF00069	0.381
MOD_Plk_4	105	111	PF00069	0.450
MOD_Plk_4	279	285	PF00069	0.390
MOD_ProDKin_1	131	137	PF00069	0.546
MOD_ProDKin_1	44	50	PF00069	0.621
MOD_SUMO_rev_2	73	82	PF00179	0.505
MOD_SUMO_rev_2	8	16	PF00179	0.575
TRG_ENDOCYTIC_2	118	121	PF00928	0.512
TRG_ENDOCYTIC_2	126	129	PF00928	0.453
TRG_ENDOCYTIC_2	180	183	PF00928	0.515
TRG_ENDOCYTIC_2	206	209	PF00928	0.435
TRG_ENDOCYTIC_2	331	334	PF00928	0.448
TRG_NES_CRM1_1	303	315	PF08389	0.378

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0S4JTD3	Bodo saltans	27%	84%
A0A1X0NTC3	Trypanosomatidae	41%	100%
A0A3Q8IAW1	Leishmania donovani	80%	100%
A0A3R7N4K3	Trypanosoma rangeli	46%	100%
A0A3R7RDH3	Trypanosoma rangeli	40%	100%
A0A422NZS9	Trypanosoma rangeli	27%	95%
A4HXE2	Leishmania infantum	80%	100%
E9AR37	Leishmania mexicana (strain MHOM/GT/2001/U1103)	80%	100%
Q4QEC6	Leishmania major	79%	100%
V5BE10	Trypanosoma cruzi	46%	100%