Separase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Separase

Gene product:

eukaryotic translation initiation factor 3 subunit a

Species:

Leishmania braziliensis

UniProt:

A4H8U7_LEIBR

TriTrypDb:

LbrM.17.0020 , LBRM2903_170005400

Length:

774

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 2
Forrest at al. (procyclic)	no	yes: 2
Silverman et al.	no	yes: 2
Pissara et al.	yes	yes: 10

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 4

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 4

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005852	eukaryotic translation initiation factor 3 complex	2	12
GO:0032991	protein-containing complex	1	12
GO:0005737	cytoplasm	2	1
GO:0043614	multi-eIF complex	3	1
GO:0071540	eukaryotic translation initiation factor 3 complex, eIF3e	3	1
GO:0071541	eukaryotic translation initiation factor 3 complex, eIF3m	3	1
GO:0110165	cellular anatomical entity	1	1
GO:1990904	ribonucleoprotein complex	2	1

Expansion

Sequence features

A4H8U7

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H8U7

Function

Biological processes
Term	Name	Level	Count
GO:0001732	formation of cytoplasmic translation initiation complex	7	1
GO:0002183	cytoplasmic translational initiation	4	1
GO:0002188	translation reinitiation	5	1
GO:0006413	translational initiation	3	1
GO:0008152	metabolic process	1	1
GO:0009987	cellular process	1	1
GO:0016043	cellular component organization	3	1
GO:0022607	cellular component assembly	4	1
GO:0022618	ribonucleoprotein complex assembly	6	1
GO:0043933	protein-containing complex organization	4	1
GO:0044237	cellular metabolic process	2	1
GO:0065003	protein-containing complex assembly	5	1
GO:0071826	ribonucleoprotein complex subunit organization	5	1
GO:0071840	cellular component organization or biogenesis	2	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	11
GO:0003743	translation initiation factor activity	4	11
GO:0005488	binding	1	11
GO:0008135	translation factor activity, RNA binding	3	11
GO:0045182	translation regulator activity	1	11
GO:0090079	translation regulator activity, nucleic acid binding	2	11
GO:0097159	organic cyclic compound binding	2	11
GO:1901363	heterocyclic compound binding	2	11
GO:0003723	RNA binding	4	1
GO:0003729	mRNA binding	5	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	29	33	PF00656	0.488
CLV_C14_Caspase3-7	490	494	PF00656	0.578
CLV_NRD_NRD_1	151	153	PF00675	0.564
CLV_NRD_NRD_1	590	592	PF00675	0.630
CLV_NRD_NRD_1	601	603	PF00675	0.498
CLV_NRD_NRD_1	606	608	PF00675	0.439
CLV_NRD_NRD_1	626	628	PF00675	0.478
CLV_NRD_NRD_1	663	665	PF00675	0.590
CLV_NRD_NRD_1	690	692	PF00675	0.551
CLV_NRD_NRD_1	718	720	PF00675	0.532
CLV_NRD_NRD_1	723	725	PF00675	0.501
CLV_NRD_NRD_1	74	76	PF00675	0.556
CLV_NRD_NRD_1	745	747	PF00675	0.711
CLV_NRD_NRD_1	769	771	PF00675	0.685
CLV_PCSK_KEX2_1	140	142	PF00082	0.589
CLV_PCSK_KEX2_1	151	153	PF00082	0.395
CLV_PCSK_KEX2_1	272	274	PF00082	0.503
CLV_PCSK_KEX2_1	319	321	PF00082	0.592
CLV_PCSK_KEX2_1	323	325	PF00082	0.585
CLV_PCSK_KEX2_1	537	539	PF00082	0.633
CLV_PCSK_KEX2_1	582	584	PF00082	0.596
CLV_PCSK_KEX2_1	590	592	PF00082	0.573
CLV_PCSK_KEX2_1	662	664	PF00082	0.546
CLV_PCSK_KEX2_1	690	692	PF00082	0.534
CLV_PCSK_KEX2_1	718	720	PF00082	0.616
CLV_PCSK_KEX2_1	723	725	PF00082	0.404
CLV_PCSK_KEX2_1	74	76	PF00082	0.556
CLV_PCSK_KEX2_1	747	749	PF00082	0.605
CLV_PCSK_KEX2_1	756	758	PF00082	0.494
CLV_PCSK_KEX2_1	769	771	PF00082	0.550
CLV_PCSK_PC1ET2_1	140	142	PF00082	0.589
CLV_PCSK_PC1ET2_1	272	274	PF00082	0.503
CLV_PCSK_PC1ET2_1	319	321	PF00082	0.589
CLV_PCSK_PC1ET2_1	323	325	PF00082	0.580
CLV_PCSK_PC1ET2_1	537	539	PF00082	0.734
CLV_PCSK_PC1ET2_1	582	584	PF00082	0.599
CLV_PCSK_PC1ET2_1	662	664	PF00082	0.577
CLV_PCSK_PC1ET2_1	690	692	PF00082	0.553
CLV_PCSK_PC1ET2_1	747	749	PF00082	0.603
CLV_PCSK_PC1ET2_1	756	758	PF00082	0.494
CLV_PCSK_PC7_1	719	725	PF00082	0.624
CLV_PCSK_SKI1_1	167	171	PF00082	0.477
CLV_PCSK_SKI1_1	178	182	PF00082	0.433
CLV_PCSK_SKI1_1	231	235	PF00082	0.501
CLV_PCSK_SKI1_1	3	7	PF00082	0.668
CLV_PCSK_SKI1_1	330	334	PF00082	0.388
CLV_PCSK_SKI1_1	409	413	PF00082	0.502
CLV_PCSK_SKI1_1	430	434	PF00082	0.423
CLV_PCSK_SKI1_1	623	627	PF00082	0.451
CLV_PCSK_SKI1_1	671	675	PF00082	0.627
CLV_PCSK_SKI1_1	728	732	PF00082	0.546
CLV_Separin_Metazoa	71	75	PF03568	0.546
DEG_APCC_DBOX_1	329	337	PF00400	0.392
DEG_APCC_DBOX_1	433	441	PF00400	0.495
DEG_Nend_UBRbox_2	1	3	PF02207	0.727
DEG_SPOP_SBC_1	518	522	PF00917	0.493
DEG_SPOP_SBC_1	532	536	PF00917	0.709
DEG_SPOP_SBC_1	541	545	PF00917	0.499
DEG_SPOP_SBC_1	552	556	PF00917	0.555
DOC_CKS1_1	187	192	PF01111	0.416
DOC_CKS1_1	42	47	PF01111	0.540
DOC_MAPK_DCC_7	39	48	PF00069	0.556
DOC_MAPK_gen_1	277	287	PF00069	0.540
DOC_MAPK_MEF2A_6	92	101	PF00069	0.447
DOC_MAPK_RevD_3	127	141	PF00069	0.537
DOC_PP1_RVXF_1	165	172	PF00149	0.527
DOC_PP4_FxxP_1	187	190	PF00568	0.523
DOC_PP4_FxxP_1	362	365	PF00568	0.603
DOC_USP7_MATH_1	11	15	PF00917	0.572
DOC_USP7_MATH_1	114	118	PF00917	0.440
DOC_USP7_MATH_1	33	37	PF00917	0.474
DOC_USP7_MATH_1	4	8	PF00917	0.667
DOC_USP7_MATH_1	416	420	PF00917	0.613
DOC_USP7_MATH_1	514	518	PF00917	0.705
DOC_USP7_MATH_1	519	523	PF00917	0.718
DOC_USP7_MATH_1	532	536	PF00917	0.709
DOC_USP7_MATH_1	575	579	PF00917	0.623
DOC_USP7_MATH_1	696	700	PF00917	0.453
DOC_USP7_UBL2_3	319	323	PF12436	0.597
DOC_USP7_UBL2_3	577	581	PF12436	0.605
DOC_USP7_UBL2_3	628	632	PF12436	0.506
DOC_WW_Pin1_4	186	191	PF00397	0.424
DOC_WW_Pin1_4	361	366	PF00397	0.618
DOC_WW_Pin1_4	383	388	PF00397	0.557
DOC_WW_Pin1_4	41	46	PF00397	0.558
LIG_14-3-3_CanoR_1	10	16	PF00244	0.587
LIG_14-3-3_CanoR_1	178	184	PF00244	0.482
LIG_14-3-3_CanoR_1	202	209	PF00244	0.518
LIG_14-3-3_CanoR_1	485	490	PF00244	0.572
LIG_14-3-3_CanoR_1	524	531	PF00244	0.713
LIG_14-3-3_CanoR_1	551	560	PF00244	0.576
LIG_14-3-3_CanoR_1	723	731	PF00244	0.421
LIG_Actin_WH2_2	65	83	PF00022	0.555
LIG_AP2alpha_2	346	348	PF02296	0.650
LIG_BRCT_BRCA1_1	295	299	PF00533	0.489
LIG_FHA_1	230	236	PF00498	0.490
LIG_FHA_1	240	246	PF00498	0.384
LIG_FHA_1	299	305	PF00498	0.577
LIG_FHA_1	368	374	PF00498	0.571
LIG_FHA_1	376	382	PF00498	0.502
LIG_FHA_1	484	490	PF00498	0.490
LIG_FHA_1	542	548	PF00498	0.643
LIG_FHA_1	653	659	PF00498	0.455
LIG_FHA_2	116	122	PF00498	0.599
LIG_FHA_2	292	298	PF00498	0.498
LIG_FHA_2	458	464	PF00498	0.604
LIG_FHA_2	543	549	PF00498	0.530
LIG_FHA_2	700	706	PF00498	0.667
LIG_IRF3_LxIS_1	190	197	PF10401	0.489
LIG_LIR_Apic_2	186	190	PF02991	0.484
LIG_LIR_Apic_2	359	365	PF02991	0.595
LIG_LIR_Gen_1	289	299	PF02991	0.428
LIG_LIR_Gen_1	398	407	PF02991	0.522
LIG_LIR_Gen_1	443	454	PF02991	0.580
LIG_LIR_Gen_1	501	510	PF02991	0.521
LIG_LIR_Gen_1	522	533	PF02991	0.728
LIG_LIR_Nem_3	346	351	PF02991	0.664
LIG_LIR_Nem_3	398	404	PF02991	0.516
LIG_LIR_Nem_3	443	449	PF02991	0.576
LIG_LIR_Nem_3	501	506	PF02991	0.541
LIG_LIR_Nem_3	522	528	PF02991	0.728
LIG_NRBOX	247	253	PF00104	0.493
LIG_NRBOX	653	659	PF00104	0.488
LIG_PCNA_yPIPBox_3	167	181	PF02747	0.506
LIG_PCNA_yPIPBox_3	277	288	PF02747	0.521
LIG_PCNA_yPIPBox_3	91	102	PF02747	0.506
LIG_Pex14_2	741	745	PF04695	0.642
LIG_RPA_C_Fungi	548	560	PF08784	0.618
LIG_SH2_GRB2like	464	467	PF00017	0.518
LIG_SH2_GRB2like	502	505	PF00017	0.595
LIG_SH2_NCK_1	382	386	PF00017	0.458
LIG_SH2_PTP2	446	449	PF00017	0.528
LIG_SH2_SRC	446	449	PF00017	0.573
LIG_SH2_STAP1	679	683	PF00017	0.610
LIG_SH2_STAT3	558	561	PF00017	0.618
LIG_SH2_STAT3	694	697	PF00017	0.646
LIG_SH2_STAT5	310	313	PF00017	0.448
LIG_SH2_STAT5	382	385	PF00017	0.478
LIG_SH2_STAT5	446	449	PF00017	0.502
LIG_SH2_STAT5	464	467	PF00017	0.474
LIG_SH2_STAT5	502	505	PF00017	0.614
LIG_SH2_STAT5	605	608	PF00017	0.618
LIG_SH2_STAT5	613	616	PF00017	0.576
LIG_SH2_STAT5	713	716	PF00017	0.633
LIG_SUMO_SIM_anti_2	282	289	PF11976	0.527
LIG_SUMO_SIM_anti_2	49	56	PF11976	0.510
LIG_SUMO_SIM_par_1	192	198	PF11976	0.502
LIG_SUMO_SIM_par_1	282	289	PF11976	0.320
LIG_SUMO_SIM_par_1	469	477	PF11976	0.536
LIG_TRAF2_1	223	226	PF00917	0.585
LIG_UBA3_1	372	379	PF00899	0.522
MOD_CK1_1	117	123	PF00069	0.411
MOD_CK1_1	13	19	PF00069	0.592
MOD_CK1_1	130	136	PF00069	0.296
MOD_CK1_1	364	370	PF00069	0.592
MOD_CK1_1	402	408	PF00069	0.521
MOD_CK1_1	517	523	PF00069	0.737
MOD_CK1_1	526	532	PF00069	0.522
MOD_CK1_1	611	617	PF00069	0.382
MOD_CK1_1	699	705	PF00069	0.441
MOD_CK2_1	115	121	PF00069	0.513
MOD_CK2_1	457	463	PF00069	0.575
MOD_CK2_1	542	548	PF00069	0.568
MOD_CK2_1	564	570	PF00069	0.562
MOD_CK2_1	699	705	PF00069	0.663
MOD_GlcNHglycan	129	132	PF01048	0.345
MOD_GlcNHglycan	288	291	PF01048	0.469
MOD_GlcNHglycan	353	357	PF01048	0.531
MOD_GlcNHglycan	440	443	PF01048	0.529
MOD_GlcNHglycan	521	524	PF01048	0.708
MOD_GlcNHglycan	645	648	PF01048	0.593
MOD_GlcNHglycan	77	80	PF01048	0.527
MOD_GSK3_1	194	201	PF00069	0.453
MOD_GSK3_1	229	236	PF00069	0.520
MOD_GSK3_1	310	317	PF00069	0.451
MOD_GSK3_1	332	339	PF00069	0.421
MOD_GSK3_1	361	368	PF00069	0.562
MOD_GSK3_1	481	488	PF00069	0.483
MOD_GSK3_1	509	516	PF00069	0.700
MOD_GSK3_1	519	526	PF00069	0.747
MOD_N-GLC_1	159	164	PF02516	0.467
MOD_N-GLC_1	173	178	PF02516	0.397
MOD_N-GLC_1	399	404	PF02516	0.534
MOD_N-GLC_1	457	462	PF02516	0.622
MOD_NEK2_1	115	120	PF00069	0.538
MOD_NEK2_1	194	199	PF00069	0.463
MOD_NEK2_1	234	239	PF00069	0.498
MOD_NEK2_1	407	412	PF00069	0.499
MOD_NEK2_1	440	445	PF00069	0.515
MOD_NEK2_1	491	496	PF00069	0.547
MOD_NEK2_1	741	746	PF00069	0.646
MOD_NEK2_1	80	85	PF00069	0.516
MOD_NEK2_2	173	178	PF00069	0.494
MOD_NEK2_2	300	305	PF00069	0.449
MOD_PIKK_1	80	86	PF00454	0.516
MOD_PK_1	553	559	PF00069	0.593
MOD_PKA_1	167	173	PF00069	0.520
MOD_PKA_1	723	729	PF00069	0.395
MOD_PKA_2	11	17	PF00069	0.557
MOD_PKA_2	491	497	PF00069	0.607
MOD_PKA_2	523	529	PF00069	0.728
MOD_PKA_2	552	558	PF00069	0.512
MOD_PKA_2	723	729	PF00069	0.395
MOD_PKA_2	80	86	PF00069	0.507
MOD_PKB_1	350	358	PF00069	0.393
MOD_PKB_1	551	559	PF00069	0.594
MOD_Plk_1	159	165	PF00069	0.469
MOD_Plk_1	173	179	PF00069	0.401
MOD_Plk_1	229	235	PF00069	0.579
MOD_Plk_1	402	408	PF00069	0.559
MOD_Plk_1	457	463	PF00069	0.478
MOD_Plk_1	474	480	PF00069	0.572
MOD_Plk_1	526	532	PF00069	0.712
MOD_Plk_1	638	644	PF00069	0.514
MOD_Plk_1	652	658	PF00069	0.465
MOD_Plk_1	741	747	PF00069	0.575
MOD_Plk_1	86	92	PF00069	0.559
MOD_Plk_2-3	476	482	PF00069	0.585
MOD_Plk_4	167	173	PF00069	0.501
MOD_Plk_4	300	306	PF00069	0.465
MOD_Plk_4	368	374	PF00069	0.505
MOD_Plk_4	498	504	PF00069	0.581
MOD_Plk_4	553	559	PF00069	0.517
MOD_Plk_4	638	644	PF00069	0.557
MOD_Plk_4	93	99	PF00069	0.498
MOD_ProDKin_1	186	192	PF00069	0.418
MOD_ProDKin_1	361	367	PF00069	0.614
MOD_ProDKin_1	383	389	PF00069	0.548
MOD_ProDKin_1	41	47	PF00069	0.549
MOD_SUMO_for_1	596	599	PF00179	0.604
MOD_SUMO_rev_2	20	30	PF00179	0.520
MOD_SUMO_rev_2	570	578	PF00179	0.648
MOD_SUMO_rev_2	667	673	PF00179	0.633
TRG_DiLeu_BaEn_1	49	54	PF01217	0.512
TRG_DiLeu_BaEn_1	65	70	PF01217	0.522
TRG_DiLeu_BaEn_1	653	658	PF01217	0.461
TRG_DiLeu_BaEn_1	759	764	PF01217	0.522
TRG_DiLeu_BaEn_2	229	235	PF01217	0.547
TRG_DiLeu_BaEn_4	766	772	PF01217	0.666
TRG_DiLeu_BaLyEn_6	149	154	PF01217	0.501
TRG_DiLeu_LyEn_5	653	658	PF01217	0.561
TRG_DiLeu_LyEn_5	759	764	PF01217	0.657
TRG_ENDOCYTIC_2	292	295	PF00928	0.413
TRG_ENDOCYTIC_2	446	449	PF00928	0.573
TRG_ENDOCYTIC_2	525	528	PF00928	0.730
TRG_ENDOCYTIC_2	633	636	PF00928	0.453
TRG_ER_diArg_1	150	152	PF00400	0.503
TRG_ER_diArg_1	73	75	PF00400	0.551
TRG_ER_diArg_1	745	748	PF00400	0.729
TRG_ER_diArg_1	768	770	PF00400	0.680
TRG_ER_diArg_1	99	102	PF00400	0.551
TRG_NES_CRM1_1	49	62	PF08389	0.515
TRG_NLS_MonoExtC_3	661	667	PF00514	0.612
TRG_Pf-PMV_PEXEL_1	607	612	PF00026	0.529
TRG_Pf-PMV_PEXEL_1	762	766	PF00026	0.656
TRG_Pf-PMV_PEXEL_1	769	774	PF00026	0.702

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IGW0	Leptomonas seymouri	75%	100%
A0A0S4IU09	Bodo saltans	23%	95%
A0A1X0NTC2	Trypanosomatidae	33%	100%
A0A3Q8IDU4	Leishmania donovani	93%	100%
A0A422NFC9	Trypanosoma rangeli	32%	100%
A4HX65	Leishmania infantum	93%	100%
B5RUP5	Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968)	22%	86%
C9ZTS8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	30%	100%
E9ARC2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	92%	100%
Q4QEJ8	Leishmania major	92%	100%
V5D8P7	Trypanosoma cruzi	32%	100%