Putative eukaryotic translation initiation factor 4 gamma

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

eukaryotic translation initiation factor 4 gamma 3

Species:

Leishmania braziliensis

UniProt:

A4H8T8_LEIBR

TriTrypDb:

LbrM.16.1660 , LBRM2903_160024600

Length:

543

Annotations

LeishMANIAdb interaction annotations

by homology
Contact email: opmn@cpqam.biocruz.br
Publication title: Translation initiation in Leishmania major: characterisation of multiple eIF4F subunit homologues
Publication 1st author(s): Dhalia
Publication Identifier(s): 15694484
Host organism: -1
Interaction detection method(s): pull down
Interaction type: physical association
Identification method participant A: autoradiography
Identification method participant B: autoradiography
ID(s) interactor A: P60842
ID(s) interactor B: Q4QEK5
Taxid interactor A: Homo sapiens
Taxid interactor B: Leishmania major
Biological role(s) interactor A: unspecified role
Biological role(s) interactor B: unspecified role
Experimental role(s) interactor A: bait

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	2
GO:0110165	cellular anatomical entity	1	3
GO:0005737	cytoplasm	2	1
GO:0010494	cytoplasmic stress granule	5	1
GO:0016281	eukaryotic translation initiation factor 4F complex	2	1
GO:0032991	protein-containing complex	1	1
GO:0035770	ribonucleoprotein granule	3	1
GO:0036464	cytoplasmic ribonucleoprotein granule	4	1
GO:0043226	organelle	2	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1
GO:0099080	supramolecular complex	2	1

Expansion

Sequence features

A4H8T8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H8T8

Function

Could not find GO biological_process term for this entry.

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	12
GO:0003723	RNA binding	4	12
GO:0003743	translation initiation factor activity	4	12
GO:0005488	binding	1	12
GO:0008135	translation factor activity, RNA binding	3	12
GO:0045182	translation regulator activity	1	12
GO:0090079	translation regulator activity, nucleic acid binding	2	12
GO:0097159	organic cyclic compound binding	2	12
GO:1901363	heterocyclic compound binding	2	12
GO:0003729	mRNA binding	5	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	154	158	PF00656	0.454
CLV_C14_Caspase3-7	237	241	PF00656	0.775
CLV_C14_Caspase3-7	364	368	PF00656	0.507
CLV_NRD_NRD_1	188	190	PF00675	0.286
CLV_NRD_NRD_1	364	366	PF00675	0.561
CLV_NRD_NRD_1	89	91	PF00675	0.282
CLV_PCSK_FUR_1	89	93	PF00082	0.317
CLV_PCSK_KEX2_1	89	91	PF00082	0.282
CLV_PCSK_PC1ET2_1	91	93	PF00082	0.302
CLV_PCSK_SKI1_1	198	202	PF00082	0.712
CLV_PCSK_SKI1_1	370	374	PF00082	0.515
CLV_PCSK_SKI1_1	6	10	PF00082	0.297
DEG_APCC_DBOX_1	475	483	PF00400	0.565
DEG_Nend_UBRbox_3	1	3	PF02207	0.331
DEG_SPOP_SBC_1	276	280	PF00917	0.752
DEG_SPOP_SBC_1	360	364	PF00917	0.563
DOC_MAPK_gen_1	177	185	PF00069	0.487
DOC_MAPK_gen_1	89	97	PF00069	0.476
DOC_PP1_RVXF_1	175	181	PF00149	0.476
DOC_PP1_RVXF_1	4	10	PF00149	0.451
DOC_SPAK_OSR1_1	179	183	PF12202	0.517
DOC_USP7_MATH_1	16	20	PF00917	0.501
DOC_USP7_MATH_1	234	238	PF00917	0.752
DOC_USP7_MATH_1	252	256	PF00917	0.726
DOC_USP7_MATH_1	282	286	PF00917	0.657
DOC_USP7_MATH_1	394	398	PF00917	0.383
DOC_USP7_MATH_1	465	469	PF00917	0.424
DOC_USP7_UBL2_3	6	10	PF12436	0.400
DOC_USP7_UBL2_3	71	75	PF12436	0.567
DOC_WW_Pin1_4	297	302	PF00397	0.685
DOC_WW_Pin1_4	445	450	PF00397	0.571
LIG_14-3-3_CanoR_1	121	129	PF00244	0.581
LIG_14-3-3_CanoR_1	263	270	PF00244	0.714
LIG_14-3-3_CanoR_1	311	316	PF00244	0.403
LIG_14-3-3_CanoR_1	370	376	PF00244	0.280
LIG_14-3-3_CanoR_1	413	418	PF00244	0.425
LIG_14-3-3_CanoR_1	440	444	PF00244	0.399
LIG_14-3-3_CanoR_1	45	54	PF00244	0.538
LIG_14-3-3_CanoR_1	501	507	PF00244	0.414
LIG_14-3-3_CanoR_1	63	72	PF00244	0.400
LIG_Actin_WH2_2	22	40	PF00022	0.581
LIG_APCC_ABBAyCdc20_2	428	434	PF00400	0.519
LIG_BH_BH3_1	133	149	PF00452	0.581
LIG_Clathr_ClatBox_1	305	309	PF01394	0.547
LIG_DLG_GKlike_1	413	420	PF00625	0.528
LIG_EH1_1	179	187	PF00400	0.487
LIG_EH1_1	451	459	PF00400	0.420
LIG_eIF4E_1	452	458	PF01652	0.278
LIG_FHA_1	272	278	PF00498	0.762
LIG_FHA_1	322	328	PF00498	0.609
LIG_FHA_2	112	118	PF00498	0.534
LIG_FHA_2	141	147	PF00498	0.522
LIG_FHA_2	152	158	PF00498	0.501
LIG_FHA_2	159	165	PF00498	0.345
LIG_FHA_2	362	368	PF00498	0.442
LIG_FHA_2	419	425	PF00498	0.528
LIG_FHA_2	467	473	PF00498	0.608
LIG_GBD_Chelix_1	395	403	PF00786	0.535
LIG_Integrin_isoDGR_2	261	263	PF01839	0.783
LIG_LIR_Gen_1	110	120	PF02991	0.562
LIG_LIR_Gen_1	157	166	PF02991	0.517
LIG_LIR_Gen_1	374	383	PF02991	0.414
LIG_LIR_Gen_1	412	420	PF02991	0.414
LIG_LIR_Gen_1	442	449	PF02991	0.468
LIG_LIR_Gen_1	459	467	PF02991	0.348
LIG_LIR_Gen_1	519	525	PF02991	0.499
LIG_LIR_LC3C_4	374	378	PF02991	0.519
LIG_LIR_Nem_3	157	163	PF02991	0.487
LIG_LIR_Nem_3	19	25	PF02991	0.476
LIG_LIR_Nem_3	374	378	PF02991	0.400
LIG_LIR_Nem_3	385	391	PF02991	0.339
LIG_LIR_Nem_3	442	446	PF02991	0.441
LIG_LIR_Nem_3	459	464	PF02991	0.323
LIG_LIR_Nem_3	519	523	PF02991	0.487
LIG_LIR_Nem_3	61	65	PF02991	0.577
LIG_NRBOX	136	142	PF00104	0.537
LIG_NRBOX	494	500	PF00104	0.504
LIG_PCNA_TLS_4	153	160	PF02747	0.520
LIG_PCNA_yPIPBox_3	323	332	PF02747	0.595
LIG_PCNA_yPIPBox_3	492	501	PF02747	0.520
LIG_Pex14_1	331	335	PF04695	0.426
LIG_Pex14_2	410	414	PF04695	0.381
LIG_SH2_CRK	22	26	PF00017	0.466
LIG_SH2_NCK_1	214	218	PF00017	0.684
LIG_SH2_STAP1	32	36	PF00017	0.537
LIG_SH2_STAT5	102	105	PF00017	0.537
LIG_SH2_STAT5	159	162	PF00017	0.487
LIG_SH2_STAT5	432	435	PF00017	0.474
LIG_SH3_3	298	304	PF00018	0.644
LIG_SH3_3	461	467	PF00018	0.583
LIG_SUMO_SIM_anti_2	130	138	PF11976	0.524
LIG_SUMO_SIM_anti_2	478	484	PF11976	0.564
LIG_TRAF2_1	423	426	PF00917	0.672
LIG_TRAF2_1	469	472	PF00917	0.652
LIG_TRAF2_1	76	79	PF00917	0.521
LIG_TYR_ITIM	441	446	PF00017	0.447
LIG_UBA3_1	182	190	PF00899	0.501
LIG_WRC_WIRS_1	457	462	PF05994	0.505
LIG_WW_3	174	178	PF00397	0.517
MOD_CK1_1	123	129	PF00069	0.555
MOD_CK1_1	151	157	PF00069	0.510
MOD_CK1_1	158	164	PF00069	0.522
MOD_CK1_1	359	365	PF00069	0.574
MOD_CK2_1	158	164	PF00069	0.582
MOD_CK2_1	341	347	PF00069	0.560
MOD_CK2_1	394	400	PF00069	0.385
MOD_CK2_1	418	424	PF00069	0.505
MOD_CK2_1	465	471	PF00069	0.587
MOD_CK2_1	67	73	PF00069	0.494
MOD_GlcNHglycan	215	218	PF01048	0.591
MOD_GlcNHglycan	254	257	PF01048	0.679
MOD_GlcNHglycan	266	269	PF01048	0.688
MOD_GlcNHglycan	279	282	PF01048	0.671
MOD_GlcNHglycan	284	287	PF01048	0.621
MOD_GlcNHglycan	358	361	PF01048	0.514
MOD_GlcNHglycan	392	395	PF01048	0.372
MOD_GlcNHglycan	396	399	PF01048	0.361
MOD_GSK3_1	107	114	PF00069	0.559
MOD_GSK3_1	116	123	PF00069	0.539
MOD_GSK3_1	148	155	PF00069	0.460
MOD_GSK3_1	271	278	PF00069	0.742
MOD_GSK3_1	356	363	PF00069	0.513
MOD_GSK3_1	390	397	PF00069	0.475
MOD_GSK3_1	420	427	PF00069	0.546
MOD_GSK3_1	63	70	PF00069	0.571
MOD_N-GLC_1	272	277	PF02516	0.732
MOD_N-GLC_2	336	338	PF02516	0.543
MOD_NEK2_1	107	112	PF00069	0.526
MOD_NEK2_1	116	121	PF00069	0.535
MOD_NEK2_1	140	145	PF00069	0.487
MOD_NEK2_1	148	153	PF00069	0.487
MOD_NEK2_1	277	282	PF00069	0.755
MOD_NEK2_1	418	423	PF00069	0.489
MOD_NEK2_1	439	444	PF00069	0.475
MOD_PIKK_1	45	51	PF00454	0.589
MOD_PIKK_1	63	69	PF00454	0.400
MOD_PKA_1	90	96	PF00069	0.554
MOD_PKA_2	120	126	PF00069	0.581
MOD_PKA_2	262	268	PF00069	0.743
MOD_PKA_2	418	424	PF00069	0.504
MOD_PKA_2	439	445	PF00069	0.494
MOD_Plk_1	116	122	PF00069	0.562
MOD_Plk_1	170	176	PF00069	0.400
MOD_Plk_1	272	278	PF00069	0.729
MOD_Plk_1	32	38	PF00069	0.462
MOD_Plk_2-3	73	79	PF00069	0.501
MOD_Plk_4	108	114	PF00069	0.480
MOD_Plk_4	155	161	PF00069	0.400
MOD_Plk_4	311	317	PF00069	0.515
MOD_Plk_4	32	38	PF00069	0.513
MOD_Plk_4	413	419	PF00069	0.487
MOD_Plk_4	453	459	PF00069	0.394
MOD_Plk_4	502	508	PF00069	0.470
MOD_ProDKin_1	297	303	PF00069	0.677
MOD_ProDKin_1	445	451	PF00069	0.572
MOD_SUMO_for_1	306	309	PF00179	0.577
MOD_SUMO_rev_2	207	215	PF00179	0.789
MOD_SUMO_rev_2	68	76	PF00179	0.581
TRG_DiLeu_BaEn_1	132	137	PF01217	0.581
TRG_ENDOCYTIC_2	22	25	PF00928	0.468
TRG_ENDOCYTIC_2	443	446	PF00928	0.389
TRG_ER_diArg_1	176	179	PF00400	0.496
TRG_ER_diArg_1	88	90	PF00400	0.495
TRG_NES_CRM1_1	455	470	PF08389	0.586
TRG_NES_CRM1_1	515	527	PF08389	0.545
TRG_NLS_MonoExtN_4	89	94	PF00514	0.501
TRG_Pf-PMV_PEXEL_1	11	15	PF00026	0.268
TRG_Pf-PMV_PEXEL_1	63	67	PF00026	0.381
TRG_Pf-PMV_PEXEL_1	96	100	PF00026	0.362

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PDA5	Leptomonas seymouri	65%	86%
A0A0S4IUF0	Bodo saltans	31%	84%
A0A1X0NZC7	Trypanosomatidae	39%	86%
A0A3Q8IAL0	Leishmania donovani	91%	86%
A0A422NEE1	Trypanosoma rangeli	40%	88%
A4HX58	Leishmania infantum	92%	86%
C9ZVT3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	37%	87%
E9AQX3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	90%	86%
Q4QEK5	Leishmania major	92%	100%
V5DJ79	Trypanosoma cruzi	40%	88%