DNA-(apurinic or apyrimidinic site) endonuclease

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

apurinic/apyrimidinic endonuclease-redox protein

Species:

Leishmania braziliensis

UniProt:

A4H8J0_LEIBR

TriTrypDb:

LbrM.16.0670 , LBRM2903_160013100

Length:

444

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	2
GO:0016020	membrane	2	1

Expansion

Sequence features

A4H8J0

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H8J0

PDB structure(s): 2j63_A , 2j63_B

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	11
GO:0006259	DNA metabolic process	4	11
GO:0006281	DNA repair	5	11
GO:0006725	cellular aromatic compound metabolic process	3	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0006950	response to stress	2	11
GO:0006974	DNA damage response	4	11
GO:0008152	metabolic process	1	11
GO:0009987	cellular process	1	11
GO:0033554	cellular response to stress	3	11
GO:0034641	cellular nitrogen compound metabolic process	3	11
GO:0043170	macromolecule metabolic process	3	11
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0044260	obsolete cellular macromolecule metabolic process	3	11
GO:0046483	heterocycle metabolic process	3	11
GO:0050896	response to stimulus	1	11
GO:0051716	cellular response to stimulus	2	11
GO:0071704	organic substance metabolic process	2	11
GO:0090304	nucleic acid metabolic process	4	11
GO:1901360	organic cyclic compound metabolic process	3	11
GO:0006284	base-excision repair	6	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	8
GO:0003677	DNA binding	4	8
GO:0003824	catalytic activity	1	11
GO:0004518	nuclease activity	4	11
GO:0004519	endonuclease activity	5	11
GO:0005488	binding	1	11
GO:0016787	hydrolase activity	2	11
GO:0016788	hydrolase activity, acting on ester bonds	3	11
GO:0016829	lyase activity	2	8
GO:0043167	ion binding	2	11
GO:0043169	cation binding	3	11
GO:0046872	metal ion binding	4	11
GO:0097159	organic cyclic compound binding	2	8
GO:1901363	heterocyclic compound binding	2	8
GO:0003906	DNA-(apurinic or apyrimidinic site) endonuclease activity	4	1
GO:0004527	exonuclease activity	5	1
GO:0004529	DNA exonuclease activity	5	1
GO:0004536	DNA nuclease activity	4	1
GO:0008081	phosphoric diester hydrolase activity	5	1
GO:0008296	3'-5'-DNA exonuclease activity	7	1
GO:0008309	double-stranded DNA exodeoxyribonuclease activity	7	1
GO:0008311	double-stranded DNA 3'-5' DNA exonuclease activity	8	1
GO:0008408	3'-5' exonuclease activity	6	1
GO:0016796	exonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters	6	1
GO:0016895	DNA exonuclease activity, producing 5'-phosphomonoesters	6	1
GO:0042578	phosphoric ester hydrolase activity	4	1
GO:0140097	catalytic activity, acting on DNA	3	1
GO:0140640	catalytic activity, acting on a nucleic acid	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	171	175	PF00656	0.381
CLV_C14_Caspase3-7	9	13	PF00656	0.608
CLV_NRD_NRD_1	114	116	PF00675	0.445
CLV_NRD_NRD_1	370	372	PF00675	0.291
CLV_NRD_NRD_1	442	444	PF00675	0.328
CLV_NRD_NRD_1	71	73	PF00675	0.746
CLV_PCSK_KEX2_1	195	197	PF00082	0.423
CLV_PCSK_KEX2_1	370	372	PF00082	0.291
CLV_PCSK_KEX2_1	4	6	PF00082	0.671
CLV_PCSK_KEX2_1	442	444	PF00082	0.282
CLV_PCSK_KEX2_1	71	73	PF00082	0.705
CLV_PCSK_PC1ET2_1	195	197	PF00082	0.423
CLV_PCSK_PC1ET2_1	4	6	PF00082	0.671
CLV_PCSK_PC7_1	366	372	PF00082	0.303
CLV_PCSK_SKI1_1	129	133	PF00082	0.298
CLV_PCSK_SKI1_1	139	143	PF00082	0.257
CLV_PCSK_SKI1_1	249	253	PF00082	0.258
CLV_PCSK_SKI1_1	267	271	PF00082	0.337
CLV_PCSK_SKI1_1	415	419	PF00082	0.227
DEG_APCC_DBOX_1	296	304	PF00400	0.258
DEG_APCC_DBOX_1	414	422	PF00400	0.193
DOC_CKS1_1	225	230	PF01111	0.272
DOC_CKS1_1	306	311	PF01111	0.176
DOC_CYCLIN_yClb5_NLxxxL_5	399	407	PF00134	0.339
DOC_MAPK_gen_1	36	43	PF00069	0.579
DOC_MAPK_gen_1	370	377	PF00069	0.176
DOC_MAPK_JIP1_4	157	163	PF00069	0.376
DOC_PP1_RVXF_1	127	134	PF00149	0.284
DOC_PP1_RVXF_1	399	406	PF00149	0.277
DOC_PP4_FxxP_1	222	225	PF00568	0.389
DOC_PP4_FxxP_1	424	427	PF00568	0.258
DOC_USP7_MATH_1	312	316	PF00917	0.431
DOC_USP7_MATH_1	317	321	PF00917	0.391
DOC_USP7_MATH_2	359	365	PF00917	0.258
DOC_WW_Pin1_4	18	23	PF00397	0.614
DOC_WW_Pin1_4	224	229	PF00397	0.245
DOC_WW_Pin1_4	233	238	PF00397	0.305
DOC_WW_Pin1_4	260	265	PF00397	0.397
DOC_WW_Pin1_4	305	310	PF00397	0.209
DOC_WW_Pin1_4	320	325	PF00397	0.298
DOC_WW_Pin1_4	65	70	PF00397	0.706
DOC_WW_Pin1_4	73	78	PF00397	0.646
LIG_14-3-3_CanoR_1	115	122	PF00244	0.430
LIG_14-3-3_CanoR_1	249	255	PF00244	0.277
LIG_14-3-3_CanoR_1	288	293	PF00244	0.344
LIG_14-3-3_CanoR_1	362	366	PF00244	0.260
LIG_14-3-3_CanoR_1	370	376	PF00244	0.258
LIG_14-3-3_CanoR_1	71	77	PF00244	0.640
LIG_BIR_II_1	1	5	PF00653	0.671
LIG_BRCT_BRCA1_1	353	357	PF00533	0.176
LIG_deltaCOP1_diTrp_1	105	113	PF00928	0.489
LIG_FHA_1	130	136	PF00498	0.306
LIG_FHA_1	250	256	PF00498	0.337
LIG_FHA_1	370	376	PF00498	0.286
LIG_FHA_1	44	50	PF00498	0.584
LIG_FHA_1	74	80	PF00498	0.442
LIG_FHA_2	184	190	PF00498	0.274
LIG_FHA_2	78	84	PF00498	0.475
LIG_Integrin_isoDGR_2	397	399	PF01839	0.277
LIG_LIR_Apic_2	219	225	PF02991	0.339
LIG_LIR_Apic_2	304	309	PF02991	0.176
LIG_LIR_Apic_2	422	427	PF02991	0.258
LIG_LIR_Gen_1	105	114	PF02991	0.404
LIG_LIR_Gen_1	183	193	PF02991	0.389
LIG_LIR_Nem_3	105	110	PF02991	0.397
LIG_LIR_Nem_3	183	188	PF02991	0.389
LIG_LIR_Nem_3	323	328	PF02991	0.176
LIG_LIR_Nem_3	337	343	PF02991	0.280
LIG_LIR_Nem_3	376	381	PF02991	0.258
LIG_LIR_Nem_3	408	412	PF02991	0.258
LIG_NRBOX	137	143	PF00104	0.277
LIG_PCNA_yPIPBox_3	78	88	PF02747	0.400
LIG_Pex14_1	405	409	PF04695	0.270
LIG_PTB_Apo_2	204	211	PF02174	0.164
LIG_SH2_CRK	128	132	PF00017	0.416
LIG_SH2_STAP1	340	344	PF00017	0.258
LIG_SH2_STAT3	287	290	PF00017	0.337
LIG_SH2_STAT3	299	302	PF00017	0.337
LIG_SH2_STAT5	185	188	PF00017	0.193
LIG_SH2_STAT5	274	277	PF00017	0.258
LIG_SH2_STAT5	299	302	PF00017	0.337
LIG_SH2_STAT5	409	412	PF00017	0.337
LIG_SH2_STAT5	419	422	PF00017	0.258
LIG_SH3_3	185	191	PF00018	0.329
LIG_SH3_3	222	228	PF00018	0.339
LIG_SUMO_SIM_par_1	236	241	PF11976	0.276
LIG_TRAF2_1	350	353	PF00917	0.291
LIG_TRAF2_1	358	361	PF00917	0.225
LIG_TYR_ITIM	407	412	PF00017	0.258
LIG_WRC_WIRS_1	251	256	PF05994	0.389
LIG_WRC_WIRS_1	289	294	PF05994	0.389
LIG_WRC_WIRS_1	388	393	PF05994	0.322
MOD_CDC14_SPxK_1	26	29	PF00782	0.580
MOD_CDC14_SPxK_1	68	71	PF00782	0.629
MOD_CDK_SPK_2	73	78	PF00069	0.462
MOD_CDK_SPxK_1	23	29	PF00069	0.578
MOD_CDK_SPxK_1	65	71	PF00069	0.626
MOD_CDK_SPxxK_3	23	30	PF00069	0.598
MOD_CDK_SPxxK_3	260	267	PF00069	0.176
MOD_CDK_SPxxK_3	65	72	PF00069	0.628
MOD_CK1_1	10	16	PF00069	0.741
MOD_CK1_1	17	23	PF00069	0.716
MOD_CK1_1	256	262	PF00069	0.375
MOD_CK1_1	320	326	PF00069	0.365
MOD_CK1_1	390	396	PF00069	0.389
MOD_CK2_1	18	24	PF00069	0.634
MOD_CK2_1	347	353	PF00069	0.291
MOD_CK2_1	361	367	PF00069	0.206
MOD_CK2_1	99	105	PF00069	0.458
MOD_GlcNHglycan	16	19	PF01048	0.674
MOD_GlcNHglycan	198	201	PF01048	0.276
MOD_GlcNHglycan	277	280	PF01048	0.298
MOD_GlcNHglycan	319	322	PF01048	0.391
MOD_GlcNHglycan	32	35	PF01048	0.466
MOD_GlcNHglycan	353	357	PF01048	0.283
MOD_GlcNHglycan	51	54	PF01048	0.705
MOD_GSK3_1	14	21	PF00069	0.657
MOD_GSK3_1	163	170	PF00069	0.398
MOD_GSK3_1	196	203	PF00069	0.287
MOD_GSK3_1	249	256	PF00069	0.333
MOD_GSK3_1	258	265	PF00069	0.276
MOD_GSK3_1	347	354	PF00069	0.293
MOD_GSK3_1	361	368	PF00069	0.245
MOD_GSK3_1	425	432	PF00069	0.225
MOD_GSK3_1	44	51	PF00069	0.662
MOD_GSK3_1	6	13	PF00069	0.756
MOD_GSK3_1	73	80	PF00069	0.567
MOD_N-GLC_1	144	149	PF02516	0.339
MOD_N-GLC_1	206	211	PF02516	0.418
MOD_NEK2_1	163	168	PF00069	0.268
MOD_NEK2_1	205	210	PF00069	0.389
MOD_NEK2_1	270	275	PF00069	0.277
MOD_NEK2_1	351	356	PF00069	0.243
MOD_NEK2_1	365	370	PF00069	0.242
MOD_NEK2_1	410	415	PF00069	0.258
MOD_NEK2_2	344	349	PF00069	0.389
MOD_PIKK_1	390	396	PF00454	0.406
MOD_PK_1	144	150	PF00069	0.176
MOD_PK_1	371	377	PF00069	0.339
MOD_PKA_1	370	376	PF00069	0.176
MOD_PKA_1	71	77	PF00069	0.660
MOD_PKA_2	114	120	PF00069	0.381
MOD_PKA_2	216	222	PF00069	0.431
MOD_PKA_2	361	367	PF00069	0.260
MOD_PKA_2	369	375	PF00069	0.258
MOD_PKA_2	6	12	PF00069	0.609
MOD_PKA_2	70	76	PF00069	0.702
MOD_PKB_1	5	13	PF00069	0.672
MOD_Plk_1	144	150	PF00069	0.339
MOD_Plk_1	206	212	PF00069	0.418
MOD_Plk_2-3	183	189	PF00069	0.339
MOD_Plk_2-3	361	367	PF00069	0.368
MOD_Plk_4	144	150	PF00069	0.291
MOD_Plk_4	183	189	PF00069	0.356
MOD_Plk_4	206	212	PF00069	0.311
MOD_Plk_4	250	256	PF00069	0.258
MOD_Plk_4	270	276	PF00069	0.282
MOD_Plk_4	387	393	PF00069	0.277
MOD_Plk_4	425	431	PF00069	0.376
MOD_Plk_4	51	57	PF00069	0.460
MOD_ProDKin_1	18	24	PF00069	0.616
MOD_ProDKin_1	224	230	PF00069	0.245
MOD_ProDKin_1	233	239	PF00069	0.305
MOD_ProDKin_1	260	266	PF00069	0.397
MOD_ProDKin_1	305	311	PF00069	0.209
MOD_ProDKin_1	320	326	PF00069	0.298
MOD_ProDKin_1	65	71	PF00069	0.708
MOD_ProDKin_1	73	79	PF00069	0.637
MOD_SUMO_for_1	37	40	PF00179	0.512
MOD_SUMO_rev_2	159	168	PF00179	0.258
TRG_DiLeu_BaEn_4	246	252	PF01217	0.337
TRG_ENDOCYTIC_2	128	131	PF00928	0.441
TRG_ENDOCYTIC_2	185	188	PF00928	0.277
TRG_ENDOCYTIC_2	325	328	PF00928	0.176
TRG_ENDOCYTIC_2	409	412	PF00928	0.258
TRG_ER_diArg_1	369	371	PF00400	0.291
TRG_ER_diArg_1	441	443	PF00400	0.282
TRG_Pf-PMV_PEXEL_1	379	384	PF00026	0.440

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IIW7	Leptomonas seymouri	64%	100%
A0A0S4JDZ9	Bodo saltans	48%	100%
A0A3R7KY33	Trypanosoma rangeli	52%	98%
A0A3S7WTW9	Leishmania donovani	83%	99%
A0MTA1	Danio rerio	32%	100%
A1YES6	Gorilla gorilla gorilla	31%	100%
A1YFZ3	Pan paniscus	31%	100%
A2T6Y4	Pan troglodytes	31%	100%
A2T7I6	Pongo pygmaeus	31%	100%
C9ZW21	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	58%	100%
E9AGN4	Leishmania infantum	83%	99%
E9AQM7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	85%	99%
O15922	Leishmania major	83%	100%
P23196	Bos taurus	30%	100%
P27695	Homo sapiens	32%	100%
P28352	Mus musculus	31%	100%
P43138	Rattus norvegicus	31%	100%
P45951	Arabidopsis thaliana	32%	83%
P51173	Dictyostelium discoideum	35%	100%
Q5E9N9	Bos taurus	26%	86%
Q5XF07	Arabidopsis thaliana	23%	100%
Q68G58	Mus musculus	27%	86%
Q9UBZ4	Homo sapiens	27%	86%
V5AT33	Trypanosoma cruzi	56%	100%