Putative tubulin tyrosine ligase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

tubulin tyrosine ligase, putative

Species:

Leishmania braziliensis

UniProt:

A4H8G3_LEIBR

TriTrypDb:

LbrM.16.0390 , LBRM2903_160009400

Length:

734

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005874	microtubule	6	7
GO:0005929	cilium	4	8
GO:0042995	cell projection	2	8
GO:0043226	organelle	2	8
GO:0043227	membrane-bounded organelle	3	8
GO:0099080	supramolecular complex	2	7
GO:0099081	supramolecular polymer	3	7
GO:0099512	supramolecular fiber	4	7
GO:0099513	polymeric cytoskeletal fiber	5	7
GO:0110165	cellular anatomical entity	1	8
GO:0120025	plasma membrane bounded cell projection	3	8
GO:0005737	cytoplasm	2	1

Expansion

Sequence features

A4H8G3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H8G3

Function

Biological processes
Term	Name	Level	Count
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0018095	protein polyglutamylation	7	8
GO:0018193	peptidyl-amino acid modification	5	8
GO:0018200	peptidyl-glutamic acid modification	6	8
GO:0019538	protein metabolic process	3	12
GO:0036211	protein modification process	4	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043412	macromolecule modification	4	12
GO:0044238	primary metabolic process	2	12
GO:0071704	organic substance metabolic process	2	12
GO:1901564	organonitrogen compound metabolic process	3	12
GO:0000226	microtubule cytoskeleton organization	3	2
GO:0006996	organelle organization	4	2
GO:0007010	cytoskeleton organization	5	2
GO:0007017	microtubule-based process	2	2
GO:0009987	cellular process	1	2
GO:0016043	cellular component organization	3	2
GO:0071840	cellular component organization or biogenesis	2	2

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	7
GO:0003824	catalytic activity	1	11
GO:0004835	tubulin-tyrosine ligase activity	3	8
GO:0005488	binding	1	8
GO:0005524	ATP binding	5	7
GO:0016874	ligase activity	2	11
GO:0016879	ligase activity, forming carbon-nitrogen bonds	3	8
GO:0016881	acid-amino acid ligase activity	4	8
GO:0017076	purine nucleotide binding	4	7
GO:0030554	adenyl nucleotide binding	5	7
GO:0032553	ribonucleotide binding	3	7
GO:0032555	purine ribonucleotide binding	4	7
GO:0032559	adenyl ribonucleotide binding	5	7
GO:0035639	purine ribonucleoside triphosphate binding	4	7
GO:0036094	small molecule binding	2	7
GO:0043167	ion binding	2	7
GO:0043168	anion binding	3	7
GO:0097159	organic cyclic compound binding	2	7
GO:0097367	carbohydrate derivative binding	2	7
GO:0140096	catalytic activity, acting on a protein	2	8
GO:1901265	nucleoside phosphate binding	3	7
GO:1901363	heterocyclic compound binding	2	7
GO:0005515	protein binding	2	2
GO:0008092	cytoskeletal protein binding	3	2
GO:0015631	tubulin binding	4	2
GO:0070739	protein-glutamic acid ligase activity	3	2
GO:0070740	tubulin-glutamic acid ligase activity	4	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	107	111	PF00656	0.592
CLV_NRD_NRD_1	11	13	PF00675	0.717
CLV_NRD_NRD_1	15	17	PF00675	0.587
CLV_NRD_NRD_1	19	21	PF00675	0.562
CLV_NRD_NRD_1	250	252	PF00675	0.579
CLV_NRD_NRD_1	283	285	PF00675	0.419
CLV_NRD_NRD_1	312	314	PF00675	0.289
CLV_NRD_NRD_1	405	407	PF00675	0.275
CLV_PCSK_FUR_1	248	252	PF00082	0.562
CLV_PCSK_KEX2_1	11	13	PF00082	0.676
CLV_PCSK_KEX2_1	145	147	PF00082	0.594
CLV_PCSK_KEX2_1	15	17	PF00082	0.538
CLV_PCSK_KEX2_1	19	21	PF00082	0.532
CLV_PCSK_KEX2_1	250	252	PF00082	0.579
CLV_PCSK_KEX2_1	283	285	PF00082	0.456
CLV_PCSK_KEX2_1	322	324	PF00082	0.311
CLV_PCSK_KEX2_1	404	406	PF00082	0.275
CLV_PCSK_PC1ET2_1	145	147	PF00082	0.586
CLV_PCSK_PC1ET2_1	322	324	PF00082	0.232
CLV_PCSK_PC7_1	11	17	PF00082	0.544
CLV_PCSK_SKI1_1	19	23	PF00082	0.543
CLV_PCSK_SKI1_1	319	323	PF00082	0.232
CLV_PCSK_SKI1_1	421	425	PF00082	0.254
CLV_PCSK_SKI1_1	469	473	PF00082	0.230
CLV_PCSK_SKI1_1	684	688	PF00082	0.254
CLV_PCSK_SKI1_1	696	700	PF00082	0.264
DEG_SCF_FBW7_1	22	28	PF00400	0.628
DEG_SPOP_SBC_1	358	362	PF00917	0.410
DOC_CKS1_1	22	27	PF01111	0.628
DOC_CYCLIN_yCln2_LP_2	76	82	PF00134	0.552
DOC_MAPK_gen_1	536	544	PF00069	0.491
DOC_MAPK_MEF2A_6	536	544	PF00069	0.491
DOC_MAPK_NFAT4_5	537	545	PF00069	0.491
DOC_PP1_RVXF_1	624	631	PF00149	0.480
DOC_PP1_RVXF_1	694	700	PF00149	0.458
DOC_PP4_FxxP_1	204	207	PF00568	0.518
DOC_PP4_FxxP_1	504	507	PF00568	0.405
DOC_USP7_MATH_1	138	142	PF00917	0.670
DOC_USP7_MATH_1	241	245	PF00917	0.580
DOC_USP7_MATH_1	26	30	PF00917	0.576
DOC_USP7_MATH_1	357	361	PF00917	0.463
DOC_USP7_MATH_1	4	8	PF00917	0.665
DOC_USP7_MATH_1	450	454	PF00917	0.477
DOC_USP7_MATH_1	460	464	PF00917	0.416
DOC_USP7_MATH_1	510	514	PF00917	0.532
DOC_USP7_MATH_1	570	574	PF00917	0.521
DOC_USP7_MATH_1	672	676	PF00917	0.460
DOC_USP7_MATH_1	98	102	PF00917	0.616
DOC_USP7_UBL2_3	39	43	PF12436	0.550
DOC_WW_Pin1_4	203	208	PF00397	0.609
DOC_WW_Pin1_4	21	26	PF00397	0.638
DOC_WW_Pin1_4	223	228	PF00397	0.545
DOC_WW_Pin1_4	31	36	PF00397	0.563
DOC_WW_Pin1_4	485	490	PF00397	0.531
DOC_WW_Pin1_4	491	496	PF00397	0.505
DOC_WW_Pin1_4	670	675	PF00397	0.454
LIG_14-3-3_CanoR_1	11	17	PF00244	0.575
LIG_14-3-3_CanoR_1	174	183	PF00244	0.449
LIG_14-3-3_CanoR_1	228	236	PF00244	0.528
LIG_14-3-3_CanoR_1	335	339	PF00244	0.461
LIG_14-3-3_CanoR_1	375	380	PF00244	0.462
LIG_Actin_WH2_2	621	639	PF00022	0.473
LIG_APCC_ABBA_1	395	400	PF00400	0.454
LIG_BIR_II_1	1	5	PF00653	0.543
LIG_BIR_III_2	54	58	PF00653	0.537
LIG_BIR_III_2	64	68	PF00653	0.540
LIG_BRCT_BRCA1_1	200	204	PF00533	0.503
LIG_BRCT_BRCA1_1	290	294	PF00533	0.450
LIG_BRCT_BRCA1_1	377	381	PF00533	0.475
LIG_BRCT_BRCA1_1	493	497	PF00533	0.475
LIG_deltaCOP1_diTrp_1	274	279	PF00928	0.364
LIG_eIF4E_1	526	532	PF01652	0.454
LIG_eIF4E_1	653	659	PF01652	0.454
LIG_FHA_1	12	18	PF00498	0.638
LIG_FHA_1	146	152	PF00498	0.616
LIG_FHA_1	26	32	PF00498	0.740
LIG_FHA_1	386	392	PF00498	0.454
LIG_FHA_1	406	412	PF00498	0.454
LIG_FHA_1	45	51	PF00498	0.553
LIG_FHA_1	676	682	PF00498	0.588
LIG_FHA_1	692	698	PF00498	0.434
LIG_FHA_2	175	181	PF00498	0.450
LIG_FHA_2	310	316	PF00498	0.479
LIG_FHA_2	338	344	PF00498	0.479
LIG_FHA_2	350	356	PF00498	0.496
LIG_FHA_2	678	684	PF00498	0.454
LIG_LIR_Apic_2	201	207	PF02991	0.512
LIG_LIR_Apic_2	388	392	PF02991	0.490
LIG_LIR_Apic_2	501	507	PF02991	0.405
LIG_LIR_Gen_1	274	282	PF02991	0.362
LIG_LIR_Gen_1	331	341	PF02991	0.475
LIG_LIR_Gen_1	558	569	PF02991	0.484
LIG_LIR_Nem_3	274	278	PF02991	0.410
LIG_LIR_Nem_3	315	321	PF02991	0.455
LIG_LIR_Nem_3	331	336	PF02991	0.454
LIG_LIR_Nem_3	547	552	PF02991	0.501
LIG_LIR_Nem_3	558	564	PF02991	0.530
LIG_LIR_Nem_3	649	653	PF02991	0.549
LIG_LIR_Nem_3	727	732	PF02991	0.389
LIG_MAD2	696	704	PF02301	0.454
LIG_PCNA_PIPBox_1	576	585	PF02747	0.454
LIG_PDZ_Class_2	729	734	PF00595	0.363
LIG_Pex14_2	398	402	PF04695	0.475
LIG_PTAP_UEV_1	5	10	PF05743	0.535
LIG_PTB_Apo_2	624	631	PF02174	0.454
LIG_SH2_CRK	318	322	PF00017	0.456
LIG_SH2_CRK	333	337	PF00017	0.390
LIG_SH2_CRK	389	393	PF00017	0.454
LIG_SH2_CRK	549	553	PF00017	0.491
LIG_SH2_CRK	633	637	PF00017	0.461
LIG_SH2_CRK	655	659	PF00017	0.484
LIG_SH2_NCK_1	389	393	PF00017	0.454
LIG_SH2_PTP2	543	546	PF00017	0.464
LIG_SH2_STAP1	171	175	PF00017	0.394
LIG_SH2_STAP1	655	659	PF00017	0.454
LIG_SH2_STAT5	171	174	PF00017	0.553
LIG_SH2_STAT5	324	327	PF00017	0.454
LIG_SH2_STAT5	521	524	PF00017	0.491
LIG_SH2_STAT5	526	529	PF00017	0.491
LIG_SH2_STAT5	543	546	PF00017	0.491
LIG_SH2_STAT5	555	558	PF00017	0.491
LIG_SH2_STAT5	611	614	PF00017	0.492
LIG_SH3_1	3	9	PF00018	0.537
LIG_SH3_2	6	11	PF14604	0.538
LIG_SH3_3	132	138	PF00018	0.761
LIG_SH3_3	148	154	PF00018	0.554
LIG_SH3_3	205	211	PF00018	0.551
LIG_SH3_3	29	35	PF00018	0.601
LIG_SH3_3	3	9	PF00018	0.537
LIG_SH3_3	486	492	PF00018	0.456
LIG_SH3_3	504	510	PF00018	0.405
LIG_SH3_3	695	701	PF00018	0.472
LIG_TYR_ITAM	315	336	PF00017	0.279
LIG_TYR_ITIM	316	321	PF00017	0.279
LIG_TYR_ITIM	631	636	PF00017	0.279
LIG_UBA3_1	608	614	PF00899	0.218
LIG_WRC_WIRS_1	376	381	PF05994	0.309
LIG_WRC_WIRS_1	445	450	PF05994	0.309
LIG_WW_1	700	703	PF00397	0.309
MOD_CDK_SPK_2	223	228	PF00069	0.538
MOD_CK1_1	158	164	PF00069	0.575
MOD_CK1_1	206	212	PF00069	0.618
MOD_CK1_1	34	40	PF00069	0.599
MOD_CK1_1	444	450	PF00069	0.233
MOD_CK1_1	475	481	PF00069	0.355
MOD_CK1_1	488	494	PF00069	0.314
MOD_CK1_1	500	506	PF00069	0.221
MOD_CK1_1	581	587	PF00069	0.259
MOD_CK1_1	675	681	PF00069	0.305
MOD_CK2_1	349	355	PF00069	0.268
MOD_CK2_1	471	477	PF00069	0.352
MOD_Cter_Amidation	143	146	PF01082	0.585
MOD_Cter_Amidation	17	20	PF01082	0.546
MOD_Cter_Amidation	534	537	PF01082	0.332
MOD_GlcNHglycan	114	117	PF01048	0.543
MOD_GlcNHglycan	157	160	PF01048	0.661
MOD_GlcNHglycan	163	166	PF01048	0.675
MOD_GlcNHglycan	200	203	PF01048	0.650
MOD_GlcNHglycan	219	222	PF01048	0.601
MOD_GlcNHglycan	233	236	PF01048	0.594
MOD_GlcNHglycan	259	262	PF01048	0.506
MOD_GlcNHglycan	291	294	PF01048	0.415
MOD_GlcNHglycan	345	348	PF01048	0.279
MOD_GlcNHglycan	36	39	PF01048	0.704
MOD_GlcNHglycan	364	367	PF01048	0.279
MOD_GlcNHglycan	477	480	PF01048	0.411
MOD_GlcNHglycan	482	485	PF01048	0.362
MOD_GlcNHglycan	512	515	PF01048	0.302
MOD_GlcNHglycan	6	9	PF01048	0.617
MOD_GSK3_1	154	161	PF00069	0.627
MOD_GSK3_1	198	205	PF00069	0.603
MOD_GSK3_1	21	28	PF00069	0.634
MOD_GSK3_1	285	292	PF00069	0.418
MOD_GSK3_1	339	346	PF00069	0.327
MOD_GSK3_1	358	365	PF00069	0.375
MOD_GSK3_1	381	388	PF00069	0.279
MOD_GSK3_1	444	451	PF00069	0.278
MOD_GSK3_1	471	478	PF00069	0.310
MOD_GSK3_1	491	498	PF00069	0.306
MOD_GSK3_1	505	512	PF00069	0.296
MOD_GSK3_1	517	524	PF00069	0.263
MOD_GSK3_1	670	677	PF00069	0.324
MOD_GSK3_1	716	723	PF00069	0.374
MOD_GSK3_1	98	105	PF00069	0.657
MOD_N-GLC_1	161	166	PF02516	0.583
MOD_N-GLC_1	229	234	PF02516	0.530
MOD_N-GLC_1	349	354	PF02516	0.218
MOD_N-GLC_1	595	600	PF02516	0.289
MOD_N-GLC_1	670	675	PF02516	0.281
MOD_NEK2_1	229	234	PF00069	0.574
MOD_NEK2_1	381	386	PF00069	0.312
MOD_NEK2_1	448	453	PF00069	0.287
MOD_NEK2_1	497	502	PF00069	0.307
MOD_NEK2_1	517	522	PF00069	0.341
MOD_NEK2_1	545	550	PF00069	0.279
MOD_NEK2_1	564	569	PF00069	0.279
MOD_NEK2_1	676	681	PF00069	0.281
MOD_NEK2_1	691	696	PF00069	0.279
MOD_NEK2_1	92	97	PF00069	0.575
MOD_NEK2_2	169	174	PF00069	0.517
MOD_NEK2_2	334	339	PF00069	0.279
MOD_PIKK_1	405	411	PF00454	0.279
MOD_PIKK_1	415	421	PF00454	0.279
MOD_PIKK_1	720	726	PF00454	0.317
MOD_PKA_1	11	17	PF00069	0.560
MOD_PKA_1	145	151	PF00069	0.603
MOD_PKA_1	405	411	PF00069	0.309
MOD_PKA_2	10	16	PF00069	0.787
MOD_PKA_2	145	151	PF00069	0.603
MOD_PKA_2	230	236	PF00069	0.616
MOD_PKA_2	285	291	PF00069	0.386
MOD_PKA_2	334	340	PF00069	0.310
MOD_PKA_2	405	411	PF00069	0.279
MOD_Plk_1	169	175	PF00069	0.510
MOD_Plk_1	358	364	PF00069	0.229
MOD_Plk_1	393	399	PF00069	0.253
MOD_Plk_2-3	102	108	PF00069	0.543
MOD_Plk_2-3	646	652	PF00069	0.310
MOD_Plk_4	393	399	PF00069	0.294
MOD_Plk_4	444	450	PF00069	0.316
MOD_Plk_4	517	523	PF00069	0.313
MOD_ProDKin_1	203	209	PF00069	0.610
MOD_ProDKin_1	21	27	PF00069	0.637
MOD_ProDKin_1	223	229	PF00069	0.544
MOD_ProDKin_1	31	37	PF00069	0.564
MOD_ProDKin_1	485	491	PF00069	0.387
MOD_ProDKin_1	670	676	PF00069	0.279
MOD_SUMO_rev_2	260	270	PF00179	0.490
TRG_AP2beta_CARGO_1	558	568	PF09066	0.279
TRG_DiLeu_BaEn_1	45	50	PF01217	0.530
TRG_DiLeu_BaLyEn_6	527	532	PF01217	0.279
TRG_DiLeu_BaLyEn_6	681	686	PF01217	0.279
TRG_ENDOCYTIC_2	318	321	PF00928	0.282
TRG_ENDOCYTIC_2	333	336	PF00928	0.190
TRG_ENDOCYTIC_2	543	546	PF00928	0.322
TRG_ENDOCYTIC_2	549	552	PF00928	0.345
TRG_ENDOCYTIC_2	633	636	PF00928	0.293
TRG_ENDOCYTIC_2	655	658	PF00928	0.321
TRG_ER_diArg_1	15	17	PF00400	0.550
TRG_ER_diArg_1	247	250	PF00400	0.595
TRG_ER_diArg_1	282	284	PF00400	0.429
TRG_ER_diArg_1	370	373	PF00400	0.267
TRG_ER_diArg_1	404	406	PF00400	0.309
TRG_ER_diArg_1	715	718	PF00400	0.364
TRG_NES_CRM1_1	577	592	PF08389	0.279
TRG_Pf-PMV_PEXEL_1	323	327	PF00026	0.279
TRG_Pf-PMV_PEXEL_1	684	688	PF00026	0.279
TRG_Pf-PMV_PEXEL_1	94	99	PF00026	0.621

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PDX5	Leptomonas seymouri	58%	90%
A0A3Q8IDL7	Leishmania donovani	24%	100%
A0A3S5H6X0	Leishmania donovani	72%	90%
A4HWT7	Leishmania infantum	72%	90%
A4HYN9	Leishmania infantum	24%	100%
E9AID0	Leishmania braziliensis	25%	100%
E9AQJ5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	69%	90%
E9AUL7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	24%	100%
Q4QCW7	Leishmania major	24%	100%
Q4QEY2	Leishmania major	71%	100%