Radical SAM core domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Radical SAM superfamily/Wyosine base formation, putative

Species:

Leishmania braziliensis

UniProt:

A4H8C2_LEIBR

TriTrypDb:

LbrM.15.1540 , LBRM2903_150022500

Length:

416

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 12
NetGPI	no	yes: 0, no: 12

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	13
GO:0110165	cellular anatomical entity	1	13

Expansion

Sequence features

A4H8C2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H8C2

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	13
GO:0006396	RNA processing	6	13
GO:0006399	tRNA metabolic process	7	13
GO:0006725	cellular aromatic compound metabolic process	3	13
GO:0006807	nitrogen compound metabolic process	2	13
GO:0008033	tRNA processing	8	13
GO:0008152	metabolic process	1	13
GO:0009987	cellular process	1	13
GO:0016070	RNA metabolic process	5	13
GO:0034470	ncRNA processing	7	13
GO:0034641	cellular nitrogen compound metabolic process	3	13
GO:0034660	ncRNA metabolic process	6	13
GO:0043170	macromolecule metabolic process	3	13
GO:0044237	cellular metabolic process	2	13
GO:0044238	primary metabolic process	2	13
GO:0046483	heterocycle metabolic process	3	13
GO:0071704	organic substance metabolic process	2	13
GO:0090304	nucleic acid metabolic process	4	13
GO:1901360	organic cyclic compound metabolic process	3	13
GO:0006400	tRNA modification	6	1
GO:0009058	biosynthetic process	2	1
GO:0009451	RNA modification	5	1
GO:0031590	wybutosine metabolic process	4	1
GO:0031591	wybutosine biosynthetic process	5	1
GO:0043412	macromolecule modification	4	1
GO:1901135	carbohydrate derivative metabolic process	3	1
GO:1901137	carbohydrate derivative biosynthetic process	4	1
GO:1901564	organonitrogen compound metabolic process	3	1
GO:1901566	organonitrogen compound biosynthetic process	4	1
GO:1901576	organic substance biosynthetic process	3	1
GO:1901657	glycosyl compound metabolic process	4	1
GO:1901659	glycosyl compound biosynthetic process	5	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	13
GO:0005488	binding	1	13
GO:0016829	lyase activity	2	13
GO:0016830	carbon-carbon lyase activity	3	12
GO:0016833	oxo-acid-lyase activity	4	12
GO:0043167	ion binding	2	13
GO:0043169	cation binding	3	12
GO:0046872	metal ion binding	4	12
GO:0051536	iron-sulfur cluster binding	3	13
GO:0051539	4 iron, 4 sulfur cluster binding	4	13
GO:0051540	metal cluster binding	2	13
GO:0102521	tRNA-4-demethylwyosine synthase activity	5	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140101	catalytic activity, acting on a tRNA	4	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:0000166	nucleotide binding	3	1
GO:0010181	FMN binding	4	1
GO:0032553	ribonucleotide binding	3	1
GO:0036094	small molecule binding	2	1
GO:0043168	anion binding	3	1
GO:0097159	organic cyclic compound binding	2	1
GO:0097367	carbohydrate derivative binding	2	1
GO:1901265	nucleoside phosphate binding	3	1
GO:1901363	heterocyclic compound binding	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	231	235	PF00656	0.484
CLV_NRD_NRD_1	17	19	PF00675	0.415
CLV_NRD_NRD_1	176	178	PF00675	0.162
CLV_NRD_NRD_1	384	386	PF00675	0.468
CLV_PCSK_KEX2_1	16	18	PF00082	0.423
CLV_PCSK_KEX2_1	261	263	PF00082	0.312
CLV_PCSK_KEX2_1	377	379	PF00082	0.466
CLV_PCSK_KEX2_1	383	385	PF00082	0.435
CLV_PCSK_PC1ET2_1	16	18	PF00082	0.477
CLV_PCSK_PC1ET2_1	261	263	PF00082	0.312
CLV_PCSK_PC1ET2_1	377	379	PF00082	0.549
CLV_PCSK_PC1ET2_1	383	385	PF00082	0.511
CLV_PCSK_SKI1_1	10	14	PF00082	0.407
CLV_PCSK_SKI1_1	18	22	PF00082	0.403
CLV_PCSK_SKI1_1	195	199	PF00082	0.287
CLV_PCSK_SKI1_1	202	206	PF00082	0.209
CLV_PCSK_SKI1_1	225	229	PF00082	0.236
CLV_PCSK_SKI1_1	247	251	PF00082	0.377
CLV_PCSK_SKI1_1	261	265	PF00082	0.263
CLV_PCSK_SKI1_1	401	405	PF00082	0.620
DEG_APCC_DBOX_1	17	25	PF00400	0.406
DEG_Nend_UBRbox_3	1	3	PF02207	0.512
DEG_SCF_FBW7_1	263	268	PF00400	0.302
DOC_CKS1_1	357	362	PF01111	0.474
DOC_CYCLIN_yCln2_LP_2	200	206	PF00134	0.430
DOC_USP7_MATH_1	410	414	PF00917	0.657
DOC_USP7_MATH_1	66	70	PF00917	0.301
DOC_USP7_UBL2_3	194	198	PF12436	0.424
DOC_WW_Pin1_4	261	266	PF00397	0.301
DOC_WW_Pin1_4	356	361	PF00397	0.379
DOC_WW_Pin1_4	364	369	PF00397	0.411
DOC_WW_Pin1_4	64	69	PF00397	0.311
LIG_14-3-3_CanoR_1	229	233	PF00244	0.466
LIG_14-3-3_CanoR_1	301	306	PF00244	0.184
LIG_14-3-3_CanoR_1	327	332	PF00244	0.468
LIG_14-3-3_CanoR_1	38	44	PF00244	0.348
LIG_14-3-3_CanoR_1	401	411	PF00244	0.719
LIG_deltaCOP1_diTrp_1	207	212	PF00928	0.446
LIG_deltaCOP1_diTrp_1	90	93	PF00928	0.457
LIG_FHA_1	194	200	PF00498	0.472
LIG_FHA_1	359	365	PF00498	0.522
LIG_FHA_2	229	235	PF00498	0.485
LIG_FHA_2	402	408	PF00498	0.714
LIG_FHA_2	85	91	PF00498	0.457
LIG_Integrin_isoDGR_2	325	327	PF01839	0.297
LIG_Integrin_isoDGR_2	399	401	PF01839	0.480
LIG_LIR_Apic_2	353	358	PF02991	0.382
LIG_LIR_Apic_2	359	365	PF02991	0.368
LIG_LIR_Gen_1	152	159	PF02991	0.485
LIG_LIR_Gen_1	183	192	PF02991	0.436
LIG_LIR_Gen_1	207	216	PF02991	0.436
LIG_LIR_Gen_1	23	33	PF02991	0.260
LIG_LIR_Gen_1	282	290	PF02991	0.317
LIG_LIR_Gen_1	333	341	PF02991	0.372
LIG_LIR_Nem_3	152	156	PF02991	0.482
LIG_LIR_Nem_3	183	189	PF02991	0.436
LIG_LIR_Nem_3	207	212	PF02991	0.445
LIG_LIR_Nem_3	23	28	PF02991	0.275
LIG_LIR_Nem_3	245	249	PF02991	0.371
LIG_LIR_Nem_3	330	334	PF02991	0.316
LIG_LIR_Nem_3	335	340	PF02991	0.351
LIG_LYPXL_S_1	146	150	PF13949	0.246
LIG_LYPXL_yS_3	147	150	PF13949	0.446
LIG_MAD2	198	206	PF02301	0.461
LIG_PCNA_yPIPBox_3	321	332	PF02747	0.335
LIG_PTB_Apo_2	166	173	PF02174	0.436
LIG_SH2_CRK	25	29	PF00017	0.350
LIG_SH2_GRB2like	149	152	PF00017	0.470
LIG_SH2_NCK_1	25	29	PF00017	0.363
LIG_SH2_NCK_1	303	307	PF00017	0.335
LIG_SH2_STAT5	149	152	PF00017	0.470
LIG_SH2_STAT5	186	189	PF00017	0.436
LIG_SH2_STAT5	303	306	PF00017	0.412
LIG_SH2_STAT5	355	358	PF00017	0.384
LIG_SH3_3	138	144	PF00018	0.462
LIG_SH3_3	186	192	PF00018	0.446
LIG_SH3_3	200	206	PF00018	0.446
LIG_SH3_3	362	368	PF00018	0.292
LIG_SH3_3	92	98	PF00018	0.470
LIG_TRAF2_1	126	129	PF00917	0.527
LIG_TYR_ITIM	145	150	PF00017	0.446
LIG_TYR_ITSM	21	28	PF00017	0.266
LIG_UBA3_1	274	278	PF00899	0.317
LIG_WRC_WIRS_1	162	167	PF05994	0.452
MOD_CK1_1	161	167	PF00069	0.526
MOD_CK1_1	215	221	PF00069	0.362
MOD_CK1_1	84	90	PF00069	0.527
MOD_CK2_1	215	221	PF00069	0.528
MOD_CK2_1	304	310	PF00069	0.317
MOD_CK2_1	364	370	PF00069	0.453
MOD_CK2_1	401	407	PF00069	0.714
MOD_CK2_1	84	90	PF00069	0.457
MOD_GlcNHglycan	267	270	PF01048	0.301
MOD_GlcNHglycan	31	34	PF01048	0.307
MOD_GlcNHglycan	310	314	PF01048	0.273
MOD_GlcNHglycan	4	7	PF01048	0.527
MOD_GlcNHglycan	412	415	PF01048	0.714
MOD_GSK3_1	23	30	PF00069	0.397
MOD_GSK3_1	261	268	PF00069	0.314
MOD_GSK3_1	402	409	PF00069	0.685
MOD_LATS_1	158	164	PF00433	0.457
MOD_N-GLC_1	212	217	PF02516	0.270
MOD_N-GLC_2	74	76	PF02516	0.236
MOD_NEK2_1	105	110	PF00069	0.454
MOD_NEK2_1	212	217	PF00069	0.444
MOD_NEK2_1	228	233	PF00069	0.408
MOD_NEK2_1	27	32	PF00069	0.332
MOD_NEK2_2	20	25	PF00069	0.284
MOD_NEK2_2	51	56	PF00069	0.311
MOD_PIKK_1	180	186	PF00454	0.436
MOD_PKA_2	228	234	PF00069	0.436
MOD_Plk_1	212	218	PF00069	0.458
MOD_Plk_1	406	412	PF00069	0.722
MOD_Plk_4	136	142	PF00069	0.446
MOD_Plk_4	161	167	PF00069	0.452
MOD_Plk_4	20	26	PF00069	0.388
MOD_Plk_4	228	234	PF00069	0.462
MOD_Plk_4	270	276	PF00069	0.383
MOD_Plk_4	327	333	PF00069	0.331
MOD_Plk_4	336	342	PF00069	0.358
MOD_Plk_4	39	45	PF00069	0.317
MOD_ProDKin_1	261	267	PF00069	0.301
MOD_ProDKin_1	356	362	PF00069	0.377
MOD_ProDKin_1	364	370	PF00069	0.412
MOD_ProDKin_1	64	70	PF00069	0.310
MOD_SUMO_rev_2	239	249	PF00179	0.530
MOD_SUMO_rev_2	84	94	PF00179	0.527
MOD_SUMO_rev_2	96	101	PF00179	0.527
TRG_DiLeu_BaEn_1	152	157	PF01217	0.527
TRG_DiLeu_LyEn_5	152	157	PF01217	0.466
TRG_ENDOCYTIC_2	147	150	PF00928	0.446
TRG_ENDOCYTIC_2	186	189	PF00928	0.436
TRG_ENDOCYTIC_2	246	249	PF00928	0.382
TRG_ENDOCYTIC_2	25	28	PF00928	0.350
TRG_ENDOCYTIC_2	334	337	PF00928	0.469
TRG_ER_diArg_1	44	47	PF00400	0.354
TRG_NLS_MonoCore_2	382	387	PF00514	0.541

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I9B0	Leptomonas seymouri	81%	100%
A0A0S4IRG7	Bodo saltans	41%	73%
A0A0S4J780	Bodo saltans	62%	100%
A0A1X0NVI7	Trypanosomatidae	68%	99%
A0A3S7WTN8	Leishmania donovani	91%	100%
A0A422N5E5	Trypanosoma rangeli	68%	100%
A4HWP3	Leishmania infantum	91%	100%
C9ZXP5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	68%	100%
E9AQF1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	92%	100%
O59412	Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)	38%	100%
Q4QF25	Leishmania major	91%	100%
Q57705	Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)	35%	100%
Q9V1F9	Pyrococcus abyssi (strain GE5 / Orsay)	37%	100%
V5B6I5	Trypanosoma cruzi	71%	100%