RING-type domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ERAD-associated E3 ubiquitin-protein ligase HRD1, putative

Species:

Leishmania braziliensis

UniProt:

A4H8A9_LEIBR

TriTrypDb:

LbrM.15.1410 , LBRM2903_150021100

Length:

491

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 9
NetGPI	no	yes: 0, no: 9

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	9
GO:0110165	cellular anatomical entity	1	9

Expansion

Sequence features

A4H8A9

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H8A9

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	1
GO:0006511	ubiquitin-dependent protein catabolic process	7	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0009056	catabolic process	2	1
GO:0009057	macromolecule catabolic process	4	1
GO:0009987	cellular process	1	1
GO:0019538	protein metabolic process	3	1
GO:0019941	modification-dependent protein catabolic process	6	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043632	modification-dependent macromolecule catabolic process	5	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044248	cellular catabolic process	3	1
GO:0044260	obsolete cellular macromolecule metabolic process	3	1
GO:0044265	obsolete cellular macromolecule catabolic process	4	1
GO:0051603	proteolysis involved in protein catabolic process	5	1
GO:0071704	organic substance metabolic process	2	1
GO:1901564	organonitrogen compound metabolic process	3	1
GO:1901575	organic substance catabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	2
GO:0004842	ubiquitin-protein transferase activity	4	2
GO:0016740	transferase activity	2	2
GO:0019787	ubiquitin-like protein transferase activity	3	2
GO:0061630	ubiquitin protein ligase activity	5	1
GO:0061659	ubiquitin-like protein ligase activity	4	1
GO:0140096	catalytic activity, acting on a protein	2	2
GO:0016746	acyltransferase activity	3	1
GO:0061631	ubiquitin conjugating enzyme activity	5	1
GO:0061650	ubiquitin-like protein conjugating enzyme activity	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	269	271	PF00675	0.364
CLV_NRD_NRD_1	4	6	PF00675	0.340
CLV_NRD_NRD_1	428	430	PF00675	0.422
CLV_NRD_NRD_1	466	468	PF00675	0.385
CLV_PCSK_KEX2_1	269	271	PF00082	0.383
CLV_PCSK_KEX2_1	303	305	PF00082	0.326
CLV_PCSK_KEX2_1	4	6	PF00082	0.261
CLV_PCSK_KEX2_1	428	430	PF00082	0.422
CLV_PCSK_KEX2_1	468	470	PF00082	0.446
CLV_PCSK_PC1ET2_1	303	305	PF00082	0.336
CLV_PCSK_PC1ET2_1	468	470	PF00082	0.453
CLV_PCSK_SKI1_1	443	447	PF00082	0.544
CLV_PCSK_SKI1_1	478	482	PF00082	0.344
DEG_MDM2_SWIB_1	112	119	PF02201	0.418
DEG_SPOP_SBC_1	40	44	PF00917	0.394
DOC_CYCLIN_yCln2_LP_2	400	406	PF00134	0.578
DOC_MAPK_gen_1	475	483	PF00069	0.498
DOC_MAPK_MEF2A_6	475	483	PF00069	0.679
DOC_USP7_MATH_1	101	105	PF00917	0.207
DOC_USP7_MATH_1	216	220	PF00917	0.423
DOC_USP7_MATH_1	3	7	PF00917	0.459
DOC_USP7_MATH_1	40	44	PF00917	0.394
DOC_USP7_MATH_1	449	453	PF00917	0.691
DOC_WW_Pin1_4	363	368	PF00397	0.740
DOC_WW_Pin1_4	378	383	PF00397	0.768
DOC_WW_Pin1_4	399	404	PF00397	0.569
DOC_WW_Pin1_4	423	428	PF00397	0.752
DOC_WW_Pin1_4	429	434	PF00397	0.755
LIG_14-3-3_CanoR_1	126	131	PF00244	0.312
LIG_14-3-3_CanoR_1	192	196	PF00244	0.429
LIG_14-3-3_CanoR_1	204	210	PF00244	0.423
LIG_14-3-3_CanoR_1	4	8	PF00244	0.566
LIG_14-3-3_CanoR_1	428	433	PF00244	0.714
LIG_Actin_WH2_2	177	194	PF00022	0.418
LIG_APCC_ABBA_1	94	99	PF00400	0.630
LIG_BRCT_BRCA1_1	106	110	PF00533	0.437
LIG_BRCT_BRCA1_1	222	226	PF00533	0.365
LIG_BRCT_BRCA1_1	41	45	PF00533	0.265
LIG_EH1_1	195	203	PF00400	0.286
LIG_EH1_1	86	94	PF00400	0.504
LIG_FHA_1	157	163	PF00498	0.504
LIG_FHA_1	300	306	PF00498	0.566
LIG_FHA_1	440	446	PF00498	0.787
LIG_FHA_1	76	82	PF00498	0.583
LIG_FHA_2	162	168	PF00498	0.621
LIG_FHA_2	26	32	PF00498	0.539
LIG_FHA_2	277	283	PF00498	0.594
LIG_FHA_2	366	372	PF00498	0.730
LIG_GBD_Chelix_1	12	20	PF00786	0.207
LIG_LIR_Gen_1	174	185	PF02991	0.522
LIG_LIR_Gen_1	194	203	PF02991	0.413
LIG_LIR_Gen_1	263	271	PF02991	0.555
LIG_LIR_Gen_1	290	298	PF02991	0.522
LIG_LIR_Gen_1	31	40	PF02991	0.264
LIG_LIR_Gen_1	63	74	PF02991	0.446
LIG_LIR_Nem_3	106	112	PF02991	0.399
LIG_LIR_Nem_3	113	119	PF02991	0.377
LIG_LIR_Nem_3	174	180	PF02991	0.496
LIG_LIR_Nem_3	194	200	PF02991	0.403
LIG_LIR_Nem_3	223	229	PF02991	0.366
LIG_LIR_Nem_3	263	267	PF02991	0.540
LIG_LIR_Nem_3	28	32	PF02991	0.380
LIG_LIR_Nem_3	391	396	PF02991	0.716
LIG_LIR_Nem_3	42	48	PF02991	0.301
LIG_LIR_Nem_3	461	466	PF02991	0.539
LIG_LIR_Nem_3	63	69	PF02991	0.368
LIG_LIR_Nem_3	82	87	PF02991	0.611
LIG_MLH1_MIPbox_1	106	110	PF16413	0.460
LIG_PCNA_yPIPBox_3	204	213	PF02747	0.266
LIG_Pex14_2	112	116	PF04695	0.410
LIG_Pex14_2	15	19	PF04695	0.246
LIG_SH2_CRK	177	181	PF00017	0.413
LIG_SH2_CRK	183	187	PF00017	0.389
LIG_SH2_CRK	230	234	PF00017	0.460
LIG_SH2_GRB2like	25	28	PF00017	0.546
LIG_SH2_SRC	230	233	PF00017	0.469
LIG_SH2_STAP1	177	181	PF00017	0.355
LIG_SH2_STAP1	230	234	PF00017	0.469
LIG_SH2_STAP1	25	29	PF00017	0.434
LIG_SH2_STAP1	293	297	PF00017	0.536
LIG_SH2_STAP1	33	37	PF00017	0.308
LIG_SH2_STAT3	396	399	PF00017	0.726
LIG_SH2_STAT3	97	100	PF00017	0.640
LIG_SH2_STAT5	10	13	PF00017	0.321
LIG_SH2_STAT5	146	149	PF00017	0.350
LIG_SH2_STAT5	232	235	PF00017	0.341
LIG_SH2_STAT5	328	331	PF00017	0.471
LIG_SH3_3	427	433	PF00018	0.785
LIG_SUMO_SIM_anti_2	199	204	PF11976	0.257
LIG_SUMO_SIM_par_1	183	190	PF11976	0.460
LIG_SUMO_SIM_par_1	46	52	PF11976	0.379
LIG_TRAF2_1	368	371	PF00917	0.632
LIG_TRAF2_1	74	77	PF00917	0.619
LIG_TYR_ITIM	181	186	PF00017	0.434
LIG_UBA3_1	157	163	PF00899	0.533
LIG_Vh1_VBS_1	49	67	PF01044	0.207
LIG_WRC_WIRS_1	12	17	PF05994	0.440
LIG_WRC_WIRS_1	209	214	PF05994	0.318
LIG_WRC_WIRS_1	81	86	PF05994	0.595
MOD_CDC14_SPxK_1	426	429	PF00782	0.763
MOD_CDK_SPK_2	423	428	PF00069	0.608
MOD_CDK_SPxK_1	423	429	PF00069	0.763
MOD_CK1_1	104	110	PF00069	0.393
MOD_CK1_1	138	144	PF00069	0.344
MOD_CK1_1	190	196	PF00069	0.377
MOD_CK1_1	402	408	PF00069	0.636
MOD_CK1_1	432	438	PF00069	0.711
MOD_CK1_1	439	445	PF00069	0.738
MOD_CK1_1	75	81	PF00069	0.583
MOD_CK2_1	161	167	PF00069	0.622
MOD_CK2_1	216	222	PF00069	0.397
MOD_CK2_1	25	31	PF00069	0.531
MOD_CK2_1	365	371	PF00069	0.680
MOD_CK2_1	470	476	PF00069	0.604
MOD_DYRK1A_RPxSP_1	429	433	PF00069	0.621
MOD_GlcNHglycan	103	106	PF01048	0.421
MOD_GlcNHglycan	138	141	PF01048	0.431
MOD_GlcNHglycan	236	239	PF01048	0.405
MOD_GlcNHglycan	258	261	PF01048	0.399
MOD_GlcNHglycan	33	36	PF01048	0.438
MOD_GlcNHglycan	404	407	PF01048	0.514
MOD_GSK3_1	121	128	PF00069	0.436
MOD_GSK3_1	129	136	PF00069	0.369
MOD_GSK3_1	171	178	PF00069	0.568
MOD_GSK3_1	186	193	PF00069	0.350
MOD_GSK3_1	216	223	PF00069	0.324
MOD_GSK3_1	428	435	PF00069	0.704
MOD_GSK3_1	437	444	PF00069	0.691
MOD_GSK3_1	75	82	PF00069	0.570
MOD_LATS_1	441	447	PF00433	0.618
MOD_N-GLC_1	40	45	PF02516	0.558
MOD_N-GLC_1	72	77	PF02516	0.360
MOD_NEK2_1	103	108	PF00069	0.317
MOD_NEK2_1	110	115	PF00069	0.385
MOD_NEK2_1	135	140	PF00069	0.326
MOD_NEK2_1	161	166	PF00069	0.553
MOD_NEK2_1	175	180	PF00069	0.302
MOD_NEK2_1	185	190	PF00069	0.309
MOD_NEK2_1	191	196	PF00069	0.267
MOD_NEK2_1	234	239	PF00069	0.370
MOD_NEK2_1	39	44	PF00069	0.358
MOD_NEK2_1	60	65	PF00069	0.242
MOD_NEK2_1	80	85	PF00069	0.524
MOD_PKA_1	428	434	PF00069	0.622
MOD_PKA_2	125	131	PF00069	0.311
MOD_PKA_2	191	197	PF00069	0.460
MOD_PKA_2	287	293	PF00069	0.427
MOD_PKA_2	3	9	PF00069	0.566
MOD_PKA_2	428	434	PF00069	0.784
MOD_PKA_2	470	476	PF00069	0.604
MOD_Plk_1	40	46	PF00069	0.305
MOD_Plk_1	75	81	PF00069	0.583
MOD_Plk_4	104	110	PF00069	0.339
MOD_Plk_4	11	17	PF00069	0.299
MOD_Plk_4	156	162	PF00069	0.471
MOD_Plk_4	175	181	PF00069	0.180
MOD_Plk_4	196	202	PF00069	0.371
MOD_Plk_4	205	211	PF00069	0.402
MOD_Plk_4	220	226	PF00069	0.303
MOD_Plk_4	54	60	PF00069	0.281
MOD_ProDKin_1	363	369	PF00069	0.741
MOD_ProDKin_1	378	384	PF00069	0.765
MOD_ProDKin_1	399	405	PF00069	0.572
MOD_ProDKin_1	423	429	PF00069	0.754
MOD_SUMO_for_1	458	461	PF00179	0.586
MOD_SUMO_rev_2	167	174	PF00179	0.669
TRG_DiLeu_BaEn_1	76	81	PF01217	0.580
TRG_DiLeu_BaEn_2	221	227	PF01217	0.418
TRG_DiLeu_BaEn_4	76	82	PF01217	0.613
TRG_DiLeu_BaLyEn_6	237	242	PF01217	0.354
TRG_ENDOCYTIC_2	146	149	PF00928	0.345
TRG_ENDOCYTIC_2	177	180	PF00928	0.331
TRG_ENDOCYTIC_2	183	186	PF00928	0.303
TRG_ENDOCYTIC_2	230	233	PF00928	0.408
TRG_ENDOCYTIC_2	29	32	PF00928	0.389
TRG_ENDOCYTIC_2	293	296	PF00928	0.505
TRG_ENDOCYTIC_2	33	36	PF00928	0.340
TRG_ER_diArg_1	269	272	PF00400	0.595
TRG_ER_diArg_1	427	429	PF00400	0.612
TRG_ER_diArg_1	466	469	PF00400	0.585
TRG_NLS_MonoExtC_3	466	471	PF00514	0.499
TRG_NLS_MonoExtN_4	465	471	PF00514	0.500
TRG_Pf-PMV_PEXEL_1	468	472	PF00026	0.448

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I9A3	Leptomonas seymouri	49%	91%
A0A1X0NU29	Trypanosomatidae	33%	93%
A0A3R7MBX1	Trypanosoma rangeli	32%	92%
A0A3S5H6W0	Leishmania donovani	78%	100%
A4HWM9	Leishmania infantum	78%	100%
C9ZXQ9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	31%	100%
E9AQD6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	78%	100%
O74757	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	26%	73%
Q4QF39	Leishmania major	77%	97%
Q5Z880	Oryza sativa subsp. japonica	31%	98%
Q6NPT7	Arabidopsis thaliana	26%	100%
Q95SP2	Drosophila melanogaster	28%	78%
Q9LW77	Arabidopsis thaliana	26%	100%
V5BUE9	Trypanosoma cruzi	29%	92%