Ubiquitin-like modifier-activating enzyme 5

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

E1-like ubiquitin-activating enzyme, putative

Species:

Leishmania braziliensis

UniProt:

A4H870_LEIBR

TriTrypDb:

LbrM.15.1010 , LBRM2903_150013600

Length:

411

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 25

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0005739	mitochondrion	5	1
GO:0005829	cytosol	2	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4H870

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H870

Function

Biological processes
Term	Name	Level	Count
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0019538	protein metabolic process	3	1
GO:0032446	protein modification by small protein conjugation	6	1
GO:0036211	protein modification process	4	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0044238	primary metabolic process	2	1
GO:0070647	protein modification by small protein conjugation or removal	5	1
GO:0071569	protein ufmylation	7	1
GO:0071704	organic substance metabolic process	2	1
GO:1901564	organonitrogen compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0005488	binding	1	11
GO:0008641	ubiquitin-like modifier activating enzyme activity	2	12
GO:0016874	ligase activity	2	12
GO:0016877	ligase activity, forming carbon-sulfur bonds	3	12
GO:0043167	ion binding	2	11
GO:0043169	cation binding	3	11
GO:0046872	metal ion binding	4	11
GO:0140096	catalytic activity, acting on a protein	2	12
GO:0140657	ATP-dependent activity	1	12
GO:0005515	protein binding	2	1
GO:0032182	ubiquitin-like protein binding	3	1
GO:0071566	UFM1 activating enzyme activity	3	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	332	336	PF00656	0.741
CLV_C14_Caspase3-7	379	383	PF00656	0.725
CLV_NRD_NRD_1	299	301	PF00675	0.489
CLV_NRD_NRD_1	9	11	PF00675	0.619
CLV_PCSK_KEX2_1	212	214	PF00082	0.354
CLV_PCSK_PC1ET2_1	212	214	PF00082	0.354
CLV_PCSK_SKI1_1	238	242	PF00082	0.323
CLV_PCSK_SKI1_1	271	275	PF00082	0.501
CLV_PCSK_SKI1_1	391	395	PF00082	0.787
CLV_PCSK_SKI1_1	68	72	PF00082	0.370
DEG_SCF_FBW7_2	190	197	PF00400	0.370
DEG_SPOP_SBC_1	344	348	PF00917	0.539
DEG_SPOP_SBC_1	353	357	PF00917	0.525
DOC_CYCLIN_RxL_1	235	245	PF00134	0.323
DOC_CYCLIN_RxL_1	63	76	PF00134	0.354
DOC_MAPK_MEF2A_6	256	264	PF00069	0.370
DOC_PP1_RVXF_1	66	73	PF00149	0.354
DOC_PP2B_LxvP_1	187	190	PF13499	0.354
DOC_PP4_FxxP_1	86	89	PF00568	0.354
DOC_USP7_MATH_1	380	384	PF00917	0.627
DOC_USP7_UBL2_3	101	105	PF12436	0.429
DOC_WW_Pin1_4	136	141	PF00397	0.401
DOC_WW_Pin1_4	190	195	PF00397	0.354
DOC_WW_Pin1_4	3	8	PF00397	0.730
LIG_14-3-3_CanoR_1	157	161	PF00244	0.354
LIG_Actin_WH2_2	90	107	PF00022	0.248
LIG_BIR_II_1	1	5	PF00653	0.621
LIG_Clathr_ClatBox_1	69	73	PF01394	0.439
LIG_deltaCOP1_diTrp_1	124	129	PF00928	0.354
LIG_FHA_1	157	163	PF00498	0.439
LIG_FHA_1	164	170	PF00498	0.439
LIG_FHA_1	222	228	PF00498	0.354
LIG_FHA_1	356	362	PF00498	0.767
LIG_FHA_2	101	107	PF00498	0.414
LIG_FHA_2	109	115	PF00498	0.380
LIG_FHA_2	119	125	PF00498	0.299
LIG_FHA_2	377	383	PF00498	0.733
LIG_IBAR_NPY_1	20	22	PF08397	0.466
LIG_LIR_Apic_2	166	170	PF02991	0.439
LIG_LIR_Gen_1	123	133	PF02991	0.355
LIG_LIR_Gen_1	19	28	PF02991	0.370
LIG_LIR_Gen_1	35	45	PF02991	0.370
LIG_LIR_Gen_1	73	82	PF02991	0.342
LIG_LIR_LC3C_4	335	339	PF02991	0.504
LIG_LIR_Nem_3	123	129	PF02991	0.355
LIG_LIR_Nem_3	19	25	PF02991	0.370
LIG_LIR_Nem_3	247	252	PF02991	0.346
LIG_LIR_Nem_3	257	262	PF02991	0.330
LIG_LIR_Nem_3	35	40	PF02991	0.370
LIG_LIR_Nem_3	73	77	PF02991	0.342
LIG_NRBOX	236	242	PF00104	0.323
LIG_PCNA_PIPBox_1	225	234	PF02747	0.389
LIG_PCNA_PIPBox_1	79	88	PF02747	0.469
LIG_SH2_CRK	22	26	PF00017	0.370
LIG_SH2_NCK_1	74	78	PF00017	0.354
LIG_SH2_SRC	374	377	PF00017	0.643
LIG_SH2_STAP1	22	26	PF00017	0.370
LIG_SH2_STAP1	282	286	PF00017	0.531
LIG_SH2_STAT5	239	242	PF00017	0.354
LIG_SH2_STAT5	249	252	PF00017	0.354
LIG_SH2_STAT5	392	395	PF00017	0.789
LIG_SH3_3	109	115	PF00018	0.474
LIG_SH3_3	137	143	PF00018	0.411
LIG_SUMO_SIM_anti_2	142	148	PF11976	0.370
LIG_SUMO_SIM_par_1	200	206	PF11976	0.354
LIG_TRAF2_1	399	402	PF00917	0.707
LIG_TYR_ITAM	19	40	PF00017	0.389
LIG_UBA3_1	403	408	PF00899	0.616
MOD_CDK_SPxxK_3	3	10	PF00069	0.723
MOD_CK1_1	139	145	PF00069	0.501
MOD_CK1_1	2	8	PF00069	0.557
MOD_CK1_1	220	226	PF00069	0.354
MOD_CK1_1	354	360	PF00069	0.782
MOD_CK2_1	108	114	PF00069	0.412
MOD_CK2_1	118	124	PF00069	0.340
MOD_CK2_1	367	373	PF00069	0.733
MOD_CK2_1	396	402	PF00069	0.677
MOD_GlcNHglycan	12	15	PF01048	0.608
MOD_GlcNHglycan	173	176	PF01048	0.354
MOD_GlcNHglycan	181	184	PF01048	0.354
MOD_GlcNHglycan	347	350	PF01048	0.710
MOD_GlcNHglycan	358	361	PF01048	0.722
MOD_GlcNHglycan	369	372	PF01048	0.641
MOD_GlcNHglycan	382	385	PF01048	0.605
MOD_GSK3_1	127	134	PF00069	0.449
MOD_GSK3_1	217	224	PF00069	0.354
MOD_GSK3_1	351	358	PF00069	0.688
MOD_GSK3_1	376	383	PF00069	0.529
MOD_GSK3_1	392	399	PF00069	0.651
MOD_N-GLC_1	118	123	PF02516	0.439
MOD_NEK2_1	1	6	PF00069	0.631
MOD_NEK2_1	343	348	PF00069	0.539
MOD_NEK2_2	100	105	PF00069	0.248
MOD_NEK2_2	233	238	PF00069	0.389
MOD_PKA_1	10	16	PF00069	0.592
MOD_PKA_2	127	133	PF00069	0.501
MOD_PKA_2	156	162	PF00069	0.354
MOD_Plk_2-3	36	42	PF00069	0.501
MOD_Plk_2-3	73	79	PF00069	0.474
MOD_Plk_4	108	114	PF00069	0.474
MOD_Plk_4	333	339	PF00069	0.501
MOD_Plk_4	52	58	PF00069	0.370
MOD_ProDKin_1	136	142	PF00069	0.401
MOD_ProDKin_1	190	196	PF00069	0.354
MOD_ProDKin_1	3	9	PF00069	0.726
MOD_SUMO_for_1	211	214	PF00179	0.370
MOD_SUMO_rev_2	257	266	PF00179	0.501
MOD_SUMO_rev_2	330	336	PF00179	0.702
MOD_SUMO_rev_2	383	393	PF00179	0.783
TRG_ENDOCYTIC_2	22	25	PF00928	0.370
TRG_ENDOCYTIC_2	252	255	PF00928	0.354
TRG_ENDOCYTIC_2	37	40	PF00928	0.370
TRG_ENDOCYTIC_2	74	77	PF00928	0.344
TRG_NLS_MonoExtC_3	299	305	PF00514	0.611

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P2F3	Leptomonas seymouri	77%	96%
A0A0N0P8E8	Leptomonas seymouri	26%	91%
A0A0S4JGN9	Bodo saltans	69%	98%
A0A1X0NTY2	Trypanosomatidae	69%	100%
A0A3R7JZ58	Trypanosoma rangeli	69%	100%
A0A3S5H6V3	Leishmania donovani	87%	100%
A0A422NC72	Trypanosoma rangeli	28%	84%
A1A4L8	Bos taurus	26%	90%
A4HWJ5	Leishmania infantum	87%	100%
A7MAZ3	Bos taurus	50%	100%
A8XEQ8	Caenorhabditis briggsae	50%	95%
B0WQV1	Culex quinquefasciatus	61%	100%
B3NUC9	Drosophila erecta	50%	100%
B4GKQ3	Drosophila persimilis	28%	91%
B4GYC7	Drosophila persimilis	48%	100%
B4IK21	Drosophila sechellia	50%	100%
B4JBC4	Drosophila grimshawi	26%	92%
B4JIY0	Drosophila grimshawi	48%	100%
B4KI53	Drosophila mojavensis	27%	91%
B4L1K2	Drosophila mojavensis	50%	100%
B4LRB9	Drosophila virilis	26%	91%
B4M357	Drosophila virilis	56%	100%
B4NDE5	Drosophila willistoni	59%	100%
B4PYA4	Drosophila yakuba	51%	100%
B4R345	Drosophila simulans	50%	100%
B5DS72	Drosophila pseudoobscura pseudoobscura	28%	91%
C3YZ51	Branchiostoma floridae	49%	100%
C9ZXU3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	67%	100%
D0A640	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	29%	81%
D1GY43	Drosophila ananassae	49%	100%
E9AQ96	Leishmania mexicana (strain MHOM/GT/2001/U1103)	87%	100%
O23034	Arabidopsis thaliana	61%	95%
O95396	Homo sapiens	26%	89%
P91430	Caenorhabditis elegans	62%	98%
P9WMN6	Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)	24%	100%
P9WMN7	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	24%	100%
Q17DT0	Aedes aegypti	62%	100%
Q29HT0	Drosophila pseudoobscura pseudoobscura	49%	100%
Q29PG5	Drosophila pseudoobscura pseudoobscura	28%	91%
Q3KQ23	Xenopus laevis	62%	100%
Q4QF87	Leishmania major	89%	100%
Q54C02	Dictyostelium discoideum	62%	100%
Q5M7A4	Rattus norvegicus	49%	100%
Q5R8X4	Pongo abelii	50%	100%
Q6GLG7	Xenopus tropicalis	62%	100%
Q6IVA4	Gallus gallus	48%	100%
Q6K6K7	Oryza sativa subsp. japonica	60%	98%
Q8AWD2	Danio rerio	25%	90%
Q8VE47	Mus musculus	50%	100%
Q9GZZ9	Homo sapiens	63%	100%
Q9VYY3	Drosophila melanogaster	50%	100%
V5BPQ6	Trypanosoma cruzi	67%	100%
X1WER2	Danio rerio	50%	100%