Ubiquitin-conjugating enzyme

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ubiquitin-conjugating enzyme

Species:

Leishmania braziliensis

UniProt:

A4H861_LEIBR

TriTrypDb:

LbrM.15.0920 , LBRM2903_330038500

Length:

241

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4H861

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H861

Function

Biological processes
Term	Name	Level	Count
GO:0000209	protein polyubiquitination	8	1
GO:0006508	proteolysis	4	1
GO:0006511	ubiquitin-dependent protein catabolic process	7	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0009056	catabolic process	2	1
GO:0009057	macromolecule catabolic process	4	1
GO:0009987	cellular process	1	1
GO:0016567	protein ubiquitination	7	1
GO:0019538	protein metabolic process	3	1
GO:0019941	modification-dependent protein catabolic process	6	1
GO:0032446	protein modification by small protein conjugation	6	1
GO:0036211	protein modification process	4	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0043632	modification-dependent macromolecule catabolic process	5	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044248	cellular catabolic process	3	1
GO:0044260	obsolete cellular macromolecule metabolic process	3	1
GO:0044265	obsolete cellular macromolecule catabolic process	4	1
GO:0051603	proteolysis involved in protein catabolic process	5	1
GO:0070647	protein modification by small protein conjugation or removal	5	1
GO:0071704	organic substance metabolic process	2	1
GO:1901564	organonitrogen compound metabolic process	3	1
GO:1901575	organic substance catabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	11
GO:0003824	catalytic activity	1	11
GO:0005488	binding	1	11
GO:0005524	ATP binding	5	11
GO:0016740	transferase activity	2	10
GO:0017076	purine nucleotide binding	4	11
GO:0030554	adenyl nucleotide binding	5	11
GO:0032553	ribonucleotide binding	3	11
GO:0032555	purine ribonucleotide binding	4	11
GO:0032559	adenyl ribonucleotide binding	5	11
GO:0035639	purine ribonucleoside triphosphate binding	4	11
GO:0036094	small molecule binding	2	11
GO:0043167	ion binding	2	11
GO:0043168	anion binding	3	11
GO:0097159	organic cyclic compound binding	2	11
GO:0097367	carbohydrate derivative binding	2	11
GO:1901265	nucleoside phosphate binding	3	11
GO:1901363	heterocyclic compound binding	2	11
GO:0004842	ubiquitin-protein transferase activity	4	2
GO:0016874	ligase activity	2	4
GO:0019787	ubiquitin-like protein transferase activity	3	2
GO:0061631	ubiquitin conjugating enzyme activity	5	2
GO:0061650	ubiquitin-like protein conjugating enzyme activity	4	2
GO:0140096	catalytic activity, acting on a protein	2	2
GO:0016746	acyltransferase activity	3	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	179	183	PF00656	0.694
CLV_C14_Caspase3-7	199	203	PF00656	0.488
CLV_NRD_NRD_1	139	141	PF00675	0.312
CLV_NRD_NRD_1	209	211	PF00675	0.727
CLV_NRD_NRD_1	239	241	PF00675	0.616
CLV_PCSK_KEX2_1	239	241	PF00082	0.591
CLV_PCSK_KEX2_1	94	96	PF00082	0.331
CLV_PCSK_PC1ET2_1	94	96	PF00082	0.331
CLV_PCSK_SKI1_1	104	108	PF00082	0.325
CLV_PCSK_SKI1_1	111	115	PF00082	0.295
CLV_PCSK_SKI1_1	129	133	PF00082	0.312
CLV_PCSK_SKI1_1	171	175	PF00082	0.438
CLV_PCSK_SKI1_1	235	239	PF00082	0.600
CLV_PCSK_SKI1_1	70	74	PF00082	0.312
DEG_APCC_DBOX_1	103	111	PF00400	0.355
DEG_COP1_1	197	207	PF00400	0.486
DOC_CYCLIN_RxL_1	105	116	PF00134	0.312
DOC_MAPK_MEF2A_6	53	60	PF00069	0.424
DOC_SPAK_OSR1_1	53	57	PF12202	0.424
DOC_USP7_MATH_1	169	173	PF00917	0.627
DOC_USP7_MATH_1	192	196	PF00917	0.704
DOC_USP7_MATH_1	205	209	PF00917	0.540
DOC_USP7_MATH_1	30	34	PF00917	0.418
DOC_USP7_UBL2_3	232	236	PF12436	0.612
DOC_WW_Pin1_4	44	49	PF00397	0.312
LIG_14-3-3_CanoR_1	158	162	PF00244	0.573
LIG_Actin_RPEL_3	88	107	PF02755	0.355
LIG_APCC_ABBA_1	57	62	PF00400	0.297
LIG_EVH1_2	68	72	PF00568	0.424
LIG_FHA_1	172	178	PF00498	0.658
LIG_FHA_1	225	231	PF00498	0.544
LIG_FHA_1	98	104	PF00498	0.459
LIG_FHA_2	158	164	PF00498	0.634
LIG_FHA_2	177	183	PF00498	0.528
LIG_FHA_2	197	203	PF00498	0.487
LIG_Integrin_RGD_1	85	87	PF01839	0.424
LIG_LIR_Nem_3	133	138	PF02991	0.312
LIG_LIR_Nem_3	61	67	PF02991	0.319
LIG_LYPXL_yS_3	64	67	PF13949	0.312
LIG_REV1ctd_RIR_1	70	79	PF16727	0.424
LIG_SH2_CRK	145	149	PF00017	0.558
LIG_SH2_NCK_1	145	149	PF00017	0.558
LIG_SH2_STAP1	138	142	PF00017	0.424
LIG_SH2_STAT5	145	148	PF00017	0.559
LIG_SH3_2	65	70	PF14604	0.312
LIG_SH3_3	62	68	PF00018	0.312
LIG_SUMO_SIM_par_1	55	62	PF11976	0.401
MOD_CK1_1	185	191	PF00069	0.692
MOD_CK1_1	197	203	PF00069	0.409
MOD_CK2_1	157	163	PF00069	0.626
MOD_CK2_1	30	36	PF00069	0.464
MOD_GlcNHglycan	120	123	PF01048	0.296
MOD_GlcNHglycan	171	174	PF01048	0.605
MOD_GlcNHglycan	184	187	PF01048	0.628
MOD_GlcNHglycan	205	208	PF01048	0.556
MOD_GSK3_1	159	166	PF00069	0.545
MOD_GSK3_1	167	174	PF00069	0.641
MOD_GSK3_1	188	195	PF00069	0.616
MOD_GSK3_1	196	203	PF00069	0.642
MOD_GSK3_1	28	35	PF00069	0.210
MOD_N-GLC_1	80	85	PF02516	0.385
MOD_NEK2_1	196	201	PF00069	0.649
MOD_NEK2_1	72	77	PF00069	0.385
MOD_PKA_2	157	163	PF00069	0.569
MOD_PKA_2	28	34	PF00069	0.363
MOD_Plk_2-3	134	140	PF00069	0.204
MOD_ProDKin_1	44	50	PF00069	0.312
MOD_SUMO_rev_2	133	143	PF00179	0.424
TRG_DiLeu_BaEn_1	126	131	PF01217	0.312
TRG_DiLeu_LyEn_5	126	131	PF01217	0.312
TRG_ENDOCYTIC_2	145	148	PF00928	0.559
TRG_ENDOCYTIC_2	64	67	PF00928	0.303
TRG_ER_diArg_1	238	240	PF00400	0.608
TRG_NLS_MonoExtN_4	232	238	PF00514	0.717
TRG_Pf-PMV_PEXEL_1	111	116	PF00026	0.312
TRG_Pf-PMV_PEXEL_1	129	133	PF00026	0.200
TRG_Pf-PMV_PEXEL_1	9	14	PF00026	0.366

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P683	Leptomonas seymouri	69%	100%
A0A0N1I1I8	Leptomonas seymouri	29%	100%
A0A1X0NSR5	Trypanosomatidae	58%	86%
A0A3Q8IMQ9	Leishmania donovani	80%	100%
A0A3S5H604	Leishmania donovani	41%	100%
A0A422NKC5	Trypanosoma rangeli	60%	100%
A4H533	Leishmania braziliensis	41%	100%
A4HTB1	Leishmania infantum	41%	100%
A4I9B7	Leishmania infantum	80%	100%
B5DFI8	Rattus norvegicus	42%	100%
C3Z724	Branchiostoma floridae	39%	100%
C9ZJA6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	49%	97%
E9ALA2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	40%	100%
E9B2J3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	28%	100%
E9B4B7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	81%	100%
O74810	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	33%	100%
P49427	Homo sapiens	31%	100%
Q29503	Oryctolagus cuniculus	30%	100%
Q32LD2	Bos taurus	26%	100%
Q4Q3Q8	Leishmania major	79%	100%
Q4QIK2	Leishmania major	37%	100%
Q6ZWZ2	Mus musculus	30%	100%
Q712K3	Homo sapiens	30%	100%
Q7ZY08	Xenopus laevis	30%	100%
Q8CFI2	Mus musculus	31%	100%
Q9FF66	Arabidopsis thaliana	48%	96%
Q9SHI7	Arabidopsis thaliana	23%	100%
V5BAJ3	Trypanosoma cruzi	48%	100%