tRNA_SAD domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein, conserved

Species:

Leishmania braziliensis

UniProt:

A4H839_LEIBR

TriTrypDb:

LbrM.15.0700 , LBRM2903_150012900

Length:

495

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4H839

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H839

Function

Biological processes
Term	Name	Level	Count
GO:0006450	regulation of translational fidelity	3	1
GO:0065007	biological regulation	1	1
GO:0065008	regulation of biological quality	2	1
GO:0006082	organic acid metabolic process	3	4
GO:0006139	nucleobase-containing compound metabolic process	3	4
GO:0006399	tRNA metabolic process	7	4
GO:0006520	amino acid metabolic process	3	4
GO:0006725	cellular aromatic compound metabolic process	3	4
GO:0006807	nitrogen compound metabolic process	2	4
GO:0008152	metabolic process	1	4
GO:0009987	cellular process	1	4
GO:0016070	RNA metabolic process	5	4
GO:0019752	carboxylic acid metabolic process	5	4
GO:0034641	cellular nitrogen compound metabolic process	3	4
GO:0034660	ncRNA metabolic process	6	4
GO:0043038	amino acid activation	4	4
GO:0043039	tRNA aminoacylation	5	4
GO:0043170	macromolecule metabolic process	3	4
GO:0043436	oxoacid metabolic process	4	4
GO:0044237	cellular metabolic process	2	4
GO:0044238	primary metabolic process	2	4
GO:0044281	small molecule metabolic process	2	4
GO:0046483	heterocycle metabolic process	3	4
GO:0071704	organic substance metabolic process	2	4
GO:0090304	nucleic acid metabolic process	4	4
GO:1901360	organic cyclic compound metabolic process	3	4
GO:1901564	organonitrogen compound metabolic process	3	4
GO:0006418	tRNA aminoacylation for protein translation	6	3
GO:0006419	alanyl-tRNA aminoacylation	7	3

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	11
GO:0005488	binding	1	11
GO:0036094	small molecule binding	2	11
GO:0097159	organic cyclic compound binding	2	11
GO:1901265	nucleoside phosphate binding	3	11
GO:1901363	heterocyclic compound binding	2	11
GO:0002161	aminoacyl-tRNA editing activity	5	1
GO:0002196	Ser-tRNA(Ala) hydrolase activity	6	1
GO:0003824	catalytic activity	1	5
GO:0016787	hydrolase activity	2	1
GO:0016788	hydrolase activity, acting on ester bonds	3	1
GO:0052689	carboxylic ester hydrolase activity	4	1
GO:0140098	catalytic activity, acting on RNA	3	5
GO:0140101	catalytic activity, acting on a tRNA	4	5
GO:0140640	catalytic activity, acting on a nucleic acid	2	5
GO:0004812	aminoacyl-tRNA ligase activity	4	4
GO:0005524	ATP binding	5	4
GO:0016874	ligase activity	2	4
GO:0016875	ligase activity, forming carbon-oxygen bonds	3	4
GO:0017076	purine nucleotide binding	4	4
GO:0030554	adenyl nucleotide binding	5	4
GO:0032553	ribonucleotide binding	3	4
GO:0032555	purine ribonucleotide binding	4	4
GO:0032559	adenyl ribonucleotide binding	5	4
GO:0035639	purine ribonucleoside triphosphate binding	4	4
GO:0043167	ion binding	2	4
GO:0043168	anion binding	3	4
GO:0097367	carbohydrate derivative binding	2	4
GO:0004813	alanine-tRNA ligase activity	5	3
GO:0003676	nucleic acid binding	3	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	48	52	PF00656	0.462
CLV_NRD_NRD_1	10	12	PF00675	0.307
CLV_NRD_NRD_1	107	109	PF00675	0.402
CLV_NRD_NRD_1	135	137	PF00675	0.381
CLV_NRD_NRD_1	239	241	PF00675	0.583
CLV_NRD_NRD_1	349	351	PF00675	0.420
CLV_NRD_NRD_1	393	395	PF00675	0.413
CLV_PCSK_KEX2_1	10	12	PF00082	0.307
CLV_PCSK_KEX2_1	107	109	PF00082	0.402
CLV_PCSK_KEX2_1	135	137	PF00082	0.381
CLV_PCSK_KEX2_1	239	241	PF00082	0.592
CLV_PCSK_KEX2_1	349	351	PF00082	0.459
CLV_PCSK_KEX2_1	362	364	PF00082	0.398
CLV_PCSK_KEX2_1	393	395	PF00082	0.413
CLV_PCSK_PC1ET2_1	362	364	PF00082	0.477
CLV_PCSK_SKI1_1	24	28	PF00082	0.434
CLV_PCSK_SKI1_1	359	363	PF00082	0.405
CLV_PCSK_SKI1_1	44	48	PF00082	0.405
CLV_PCSK_SKI1_1	466	470	PF00082	0.334
DOC_CYCLIN_yCln2_LP_2	115	121	PF00134	0.493
DOC_CYCLIN_yCln2_LP_2	168	174	PF00134	0.435
DOC_MAPK_gen_1	359	368	PF00069	0.442
DOC_MAPK_gen_1	393	401	PF00069	0.468
DOC_MAPK_HePTP_8	380	392	PF00069	0.341
DOC_MAPK_MEF2A_6	303	311	PF00069	0.386
DOC_MAPK_MEF2A_6	383	392	PF00069	0.563
DOC_MAPK_MEF2A_6	393	401	PF00069	0.501
DOC_PP1_RVXF_1	306	312	PF00149	0.351
DOC_PP1_RVXF_1	472	478	PF00149	0.552
DOC_PP2B_LxvP_1	159	162	PF13499	0.497
DOC_USP7_MATH_1	162	166	PF00917	0.412
DOC_USP7_MATH_1	181	185	PF00917	0.417
DOC_USP7_MATH_1	244	248	PF00917	0.652
DOC_USP7_MATH_1	275	279	PF00917	0.529
DOC_USP7_MATH_1	378	382	PF00917	0.432
DOC_USP7_MATH_2	340	346	PF00917	0.312
DOC_USP7_UBL2_3	462	466	PF12436	0.553
DOC_USP7_UBL2_3	480	484	PF12436	0.446
DOC_WW_Pin1_4	114	119	PF00397	0.448
DOC_WW_Pin1_4	185	190	PF00397	0.490
DOC_WW_Pin1_4	217	222	PF00397	0.399
DOC_WW_Pin1_4	447	452	PF00397	0.556
LIG_14-3-3_CanoR_1	269	274	PF00244	0.422
LIG_14-3-3_CanoR_1	319	325	PF00244	0.315
LIG_14-3-3_CanoR_1	350	360	PF00244	0.510
LIG_14-3-3_CanoR_1	363	367	PF00244	0.471
LIG_14-3-3_CanoR_1	427	434	PF00244	0.657
LIG_Actin_WH2_2	402	417	PF00022	0.531
LIG_BIR_III_4	410	414	PF00653	0.502
LIG_BRCT_BRCA1_1	62	66	PF00533	0.366
LIG_CaM_NSCaTE_8	364	371	PF13499	0.281
LIG_FHA_1	148	154	PF00498	0.421
LIG_FHA_1	156	162	PF00498	0.413
LIG_FHA_1	25	31	PF00498	0.426
LIG_FHA_1	263	269	PF00498	0.460
LIG_FHA_1	352	358	PF00498	0.445
LIG_FHA_1	363	369	PF00498	0.411
LIG_FHA_1	385	391	PF00498	0.546
LIG_FHA_1	428	434	PF00498	0.515
LIG_FHA_1	76	82	PF00498	0.523
LIG_FHA_1	95	101	PF00498	0.420
LIG_FHA_2	286	292	PF00498	0.425
LIG_FHA_2	444	450	PF00498	0.512
LIG_FHA_2	480	486	PF00498	0.533
LIG_LIR_Gen_1	169	179	PF02991	0.399
LIG_LIR_Gen_1	225	233	PF02991	0.471
LIG_LIR_Gen_1	341	347	PF02991	0.487
LIG_LIR_Gen_1	400	409	PF02991	0.395
LIG_LIR_Gen_1	485	493	PF02991	0.508
LIG_LIR_Nem_3	169	175	PF02991	0.415
LIG_LIR_Nem_3	188	193	PF02991	0.354
LIG_LIR_Nem_3	225	230	PF02991	0.468
LIG_LIR_Nem_3	341	346	PF02991	0.515
LIG_LIR_Nem_3	400	405	PF02991	0.376
LIG_LIR_Nem_3	485	489	PF02991	0.508
LIG_LIR_Nem_3	75	79	PF02991	0.374
LIG_LIR_Nem_3	8	12	PF02991	0.515
LIG_PCNA_yPIPBox_3	146	155	PF02747	0.415
LIG_SH2_CRK	172	176	PF00017	0.467
LIG_SH2_CRK	56	60	PF00017	0.431
LIG_SH2_STAP1	56	60	PF00017	0.431
LIG_SH2_STAT5	172	175	PF00017	0.401
LIG_SH2_STAT5	193	196	PF00017	0.487
LIG_SH2_STAT5	296	299	PF00017	0.477
LIG_SH2_STAT5	310	313	PF00017	0.329
LIG_SH3_3	128	134	PF00018	0.437
LIG_SH3_3	183	189	PF00018	0.509
LIG_SH3_3	261	267	PF00018	0.506
LIG_SH3_3	385	391	PF00018	0.544
LIG_SUMO_SIM_anti_2	341	348	PF11976	0.536
LIG_SUMO_SIM_par_1	364	370	PF11976	0.532
LIG_SUMO_SIM_par_1	416	421	PF11976	0.467
LIG_SUMO_SIM_par_1	99	104	PF11976	0.551
LIG_Vh1_VBS_1	418	436	PF01044	0.350
MOD_CK1_1	117	123	PF00069	0.418
MOD_CK1_1	338	344	PF00069	0.553
MOD_CK1_1	351	357	PF00069	0.360
MOD_CK1_1	382	388	PF00069	0.466
MOD_CK1_1	91	97	PF00069	0.456
MOD_CK2_1	285	291	PF00069	0.428
MOD_CK2_1	443	449	PF00069	0.475
MOD_CK2_1	479	485	PF00069	0.498
MOD_GlcNHglycan	334	338	PF01048	0.488
MOD_GlcNHglycan	369	372	PF01048	0.377
MOD_GlcNHglycan	384	387	PF01048	0.513
MOD_GlcNHglycan	420	423	PF01048	0.428
MOD_GlcNHglycan	93	96	PF01048	0.685
MOD_GSK3_1	113	120	PF00069	0.397
MOD_GSK3_1	160	167	PF00069	0.406
MOD_GSK3_1	181	188	PF00069	0.476
MOD_GSK3_1	277	284	PF00069	0.354
MOD_GSK3_1	29	36	PF00069	0.401
MOD_GSK3_1	338	345	PF00069	0.545
MOD_GSK3_1	378	385	PF00069	0.610
MOD_GSK3_1	418	425	PF00069	0.337
MOD_GSK3_1	429	436	PF00069	0.524
MOD_GSK3_1	443	450	PF00069	0.516
MOD_GSK3_1	89	96	PF00069	0.713
MOD_NEK2_1	101	106	PF00069	0.523
MOD_NEK2_1	26	31	PF00069	0.440
MOD_NEK2_1	335	340	PF00069	0.490
MOD_NEK2_1	409	414	PF00069	0.288
MOD_NEK2_1	60	65	PF00069	0.351
MOD_NEK2_2	461	466	PF00069	0.563
MOD_NEK2_2	479	484	PF00069	0.487
MOD_PK_1	45	51	PF00069	0.475
MOD_PKA_1	198	204	PF00069	0.493
MOD_PKA_1	362	368	PF00069	0.520
MOD_PKA_2	18	24	PF00069	0.339
MOD_PKA_2	338	344	PF00069	0.545
MOD_PKA_2	348	354	PF00069	0.532
MOD_PKA_2	362	368	PF00069	0.520
MOD_PKA_2	382	388	PF00069	0.379
MOD_PKA_2	426	432	PF00069	0.648
MOD_Plk_4	18	24	PF00069	0.404
MOD_Plk_4	26	32	PF00069	0.401
MOD_Plk_4	302	308	PF00069	0.522
MOD_Plk_4	320	326	PF00069	0.270
MOD_Plk_4	342	348	PF00069	0.565
MOD_Plk_4	384	390	PF00069	0.590
MOD_Plk_4	429	435	PF00069	0.435
MOD_Plk_4	60	66	PF00069	0.350
MOD_ProDKin_1	114	120	PF00069	0.454
MOD_ProDKin_1	185	191	PF00069	0.489
MOD_ProDKin_1	217	223	PF00069	0.403
MOD_ProDKin_1	447	453	PF00069	0.553
MOD_SUMO_for_1	206	209	PF00179	0.482
MOD_SUMO_rev_2	85	89	PF00179	0.481
TRG_DiLeu_BaEn_1	485	490	PF01217	0.505
TRG_DiLeu_BaLyEn_6	96	101	PF01217	0.369
TRG_ENDOCYTIC_2	172	175	PF00928	0.385
TRG_ENDOCYTIC_2	56	59	PF00928	0.404
TRG_ER_diArg_1	106	108	PF00400	0.446
TRG_ER_diArg_1	134	136	PF00400	0.411
TRG_ER_diArg_1	255	258	PF00400	0.616
TRG_ER_diArg_1	392	394	PF00400	0.405
TRG_ER_diArg_1	405	408	PF00400	0.396
TRG_ER_diArg_1	9	11	PF00400	0.550
TRG_Pf-PMV_PEXEL_1	258	262	PF00026	0.575
TRG_Pf-PMV_PEXEL_1	329	334	PF00026	0.484

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I1V1	Leptomonas seymouri	70%	100%
A0A0S4J566	Bodo saltans	40%	100%
A0A1X0NTW0	Trypanosomatidae	42%	100%
A0A3S7WTE7	Leishmania donovani	82%	100%
A0A422NRG2	Trypanosoma rangeli	42%	100%
A4HWG6	Leishmania infantum	82%	100%
C9ZXY1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	43%	100%
E9AQ67	Leishmania mexicana (strain MHOM/GT/2001/U1103)	82%	100%
O57734	Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)	23%	100%
P53960	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	32%	100%
Q08B09	Xenopus laevis	29%	100%
Q4QFB6	Leishmania major	83%	100%
Q6DEJ5	Danio rerio	30%	100%
Q7ZYJ9	Xenopus laevis	29%	100%
Q9BTE6	Homo sapiens	29%	100%