Asparaginase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Asparaginase

Gene product:

cytoplasmic l-asparaginase i-like protein

Species:

Leishmania braziliensis

UniProt:

A4H811_LEIBR

TriTrypDb:

LbrM.15.0410 , LBRM2903_150009700

Length:

390

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4H811

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H811

Function

Biological processes
Term	Name	Level	Count
GO:0006082	organic acid metabolic process	3	11
GO:0006520	amino acid metabolic process	3	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0008152	metabolic process	1	11
GO:0009066	aspartate family amino acid metabolic process	5	11
GO:0009987	cellular process	1	11
GO:0019752	carboxylic acid metabolic process	5	11
GO:0043436	oxoacid metabolic process	4	11
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0044281	small molecule metabolic process	2	11
GO:0071704	organic substance metabolic process	2	11
GO:1901564	organonitrogen compound metabolic process	3	11
GO:1901605	alpha-amino acid metabolic process	4	11

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	11
GO:0004067	asparaginase activity	5	11
GO:0016787	hydrolase activity	2	11
GO:0016810	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds	3	11
GO:0016811	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides	4	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	73	77	PF00656	0.291
CLV_NRD_NRD_1	146	148	PF00675	0.264
CLV_NRD_NRD_1	19	21	PF00675	0.316
CLV_NRD_NRD_1	51	53	PF00675	0.285
CLV_PCSK_KEX2_1	146	148	PF00082	0.264
CLV_PCSK_KEX2_1	19	21	PF00082	0.328
CLV_PCSK_KEX2_1	250	252	PF00082	0.514
CLV_PCSK_PC1ET2_1	250	252	PF00082	0.514
CLV_PCSK_SKI1_1	19	23	PF00082	0.292
DOC_CYCLIN_yCln2_LP_2	125	131	PF00134	0.264
DOC_CYCLIN_yCln2_LP_2	212	218	PF00134	0.488
DOC_MAPK_gen_1	19	26	PF00069	0.271
DOC_MAPK_MEF2A_6	146	153	PF00069	0.264
DOC_MAPK_MEF2A_6	160	168	PF00069	0.264
DOC_MAPK_MEF2A_6	189	198	PF00069	0.354
DOC_MAPK_MEF2A_6	19	26	PF00069	0.288
DOC_MAPK_NFAT4_5	19	27	PF00069	0.283
DOC_USP7_MATH_1	187	191	PF00917	0.410
DOC_USP7_MATH_1	252	256	PF00917	0.633
DOC_USP7_MATH_1	263	267	PF00917	0.627
DOC_WW_Pin1_4	334	339	PF00397	0.283
DOC_WW_Pin1_4	361	366	PF00397	0.313
DOC_WW_Pin1_4	378	383	PF00397	0.360
LIG_14-3-3_CanoR_1	251	257	PF00244	0.567
LIG_Actin_WH2_2	44	60	PF00022	0.354
LIG_APCC_ABBA_1	92	97	PF00400	0.354
LIG_BIR_II_1	1	5	PF00653	0.421
LIG_FHA_1	16	22	PF00498	0.460
LIG_FHA_1	200	206	PF00498	0.376
LIG_FHA_1	256	262	PF00498	0.619
LIG_FHA_1	371	377	PF00498	0.425
LIG_FHA_2	243	249	PF00498	0.418
LIG_LIR_Apic_2	208	213	PF02991	0.318
LIG_LIR_Gen_1	70	75	PF02991	0.265
LIG_LIR_Nem_3	184	188	PF02991	0.284
LIG_LIR_Nem_3	190	196	PF02991	0.195
LIG_LIR_Nem_3	70	74	PF02991	0.265
LIG_LIR_Nem_3	97	102	PF02991	0.344
LIG_LYPXL_yS_3	193	196	PF13949	0.283
LIG_NRBOX	117	123	PF00104	0.283
LIG_NRBOX	242	248	PF00104	0.308
LIG_PCNA_yPIPBox_3	271	283	PF02747	0.179
LIG_SH2_CRK	85	89	PF00017	0.342
LIG_SH2_GRB2like	342	345	PF00017	0.354
LIG_SH2_STAP1	113	117	PF00017	0.264
LIG_SH2_STAP1	71	75	PF00017	0.264
LIG_SH2_STAT5	113	116	PF00017	0.264
LIG_SH2_STAT5	210	213	PF00017	0.311
LIG_SH2_STAT5	232	235	PF00017	0.248
LIG_SH2_STAT5	25	28	PF00017	0.328
LIG_SH2_STAT5	342	345	PF00017	0.302
LIG_SH2_STAT5	357	360	PF00017	0.285
LIG_SH2_STAT5	46	49	PF00017	0.264
LIG_SH2_STAT5	85	88	PF00017	0.299
LIG_SUMO_SIM_anti_2	241	248	PF11976	0.432
LIG_SUMO_SIM_par_1	126	132	PF11976	0.284
LIG_SUMO_SIM_par_1	241	248	PF11976	0.385
LIG_TYR_ITIM	191	196	PF00017	0.354
LIG_TYR_ITIM	69	74	PF00017	0.264
LIG_UBA3_1	121	127	PF00899	0.264
LIG_UBA3_1	243	250	PF00899	0.403
LIG_UBA3_1	355	361	PF00899	0.283
MOD_CDC14_SPxK_1	364	367	PF00782	0.354
MOD_CDK_SPxK_1	361	367	PF00069	0.354
MOD_CK1_1	116	122	PF00069	0.308
MOD_CK1_1	2	8	PF00069	0.502
MOD_CK1_1	255	261	PF00069	0.610
MOD_CK2_1	242	248	PF00069	0.392
MOD_GlcNHglycan	115	118	PF01048	0.308
MOD_GlcNHglycan	320	324	PF01048	0.325
MOD_GlcNHglycan	4	7	PF01048	0.573
MOD_GlcNHglycan	59	62	PF01048	0.212
MOD_GSK3_1	107	114	PF00069	0.267
MOD_GSK3_1	251	258	PF00069	0.483
MOD_GSK3_1	259	266	PF00069	0.671
MOD_GSK3_1	321	328	PF00069	0.355
MOD_GSK3_1	7	14	PF00069	0.595
MOD_N-GLC_1	168	173	PF02516	0.358
MOD_N-GLC_2	176	178	PF02516	0.309
MOD_N-GLC_2	84	86	PF02516	0.179
MOD_NEK2_1	111	116	PF00069	0.264
MOD_NEK2_1	154	159	PF00069	0.325
MOD_NEK2_1	168	173	PF00069	0.340
MOD_NEK2_1	26	31	PF00069	0.349
MOD_NEK2_2	107	112	PF00069	0.272
MOD_NEK2_2	288	293	PF00069	0.313
MOD_PIKK_1	199	205	PF00454	0.179
MOD_PIKK_1	370	376	PF00454	0.501
MOD_PKA_2	252	258	PF00069	0.437
MOD_PKA_2	57	63	PF00069	0.448
MOD_Plk_1	168	174	PF00069	0.358
MOD_Plk_1	199	205	PF00069	0.283
MOD_Plk_1	271	277	PF00069	0.474
MOD_Plk_4	116	122	PF00069	0.269
MOD_Plk_4	168	174	PF00069	0.409
MOD_Plk_4	239	245	PF00069	0.437
MOD_ProDKin_1	334	340	PF00069	0.283
MOD_ProDKin_1	361	367	PF00069	0.313
MOD_ProDKin_1	378	384	PF00069	0.378
MOD_SUMO_for_1	206	209	PF00179	0.448
TRG_DiLeu_BaEn_1	70	75	PF01217	0.264
TRG_DiLeu_BaEn_2	199	205	PF01217	0.277
TRG_DiLeu_BaLyEn_6	274	279	PF01217	0.342
TRG_ENDOCYTIC_2	185	188	PF00928	0.376
TRG_ENDOCYTIC_2	193	196	PF00928	0.241
TRG_ENDOCYTIC_2	71	74	PF00928	0.264
TRG_ENDOCYTIC_2	85	88	PF00928	0.272
TRG_ENDOCYTIC_2	99	102	PF00928	0.231
TRG_ER_diArg_1	19	21	PF00400	0.319
TRG_ER_diArg_1	225	228	PF00400	0.402
TRG_NES_CRM1_1	339	352	PF08389	0.454
TRG_NLS_MonoCore_2	249	254	PF00514	0.487
TRG_PTS1	387	390	PF00515	0.577

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HVX8	Leptomonas seymouri	72%	99%
A0A0N1PEN4	Leptomonas seymouri	30%	100%
A0A0S4J4T5	Bodo saltans	28%	96%
A0A0S4J8X9	Bodo saltans	33%	100%
A0A0S4JTX6	Bodo saltans	46%	96%
A0A1X0NTQ3	Trypanosomatidae	53%	84%
A0A1X0P1U7	Trypanosomatidae	28%	100%
A0A3Q8IA09	Leishmania donovani	82%	98%
A0A3R7KPG5	Trypanosoma rangeli	31%	100%
A0A3S7XBI7	Leishmania donovani	28%	100%
A0A422N3G9	Trypanosoma rangeli	53%	86%
A0JNU3	Mus musculus	30%	69%
A1RX40	Thermofilum pendens (strain DSM 2475 / Hrk 5)	24%	86%
A4FWR5	Methanococcus maripaludis (strain C5 / ATCC BAA-1333)	27%	93%
A4HPW0	Leishmania braziliensis	28%	100%
A4HWE1	Leishmania infantum	83%	98%
A4IDM2	Leishmania infantum	28%	100%
A4YHH3	Metallosphaera sedula (strain ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509 / TH2)	27%	89%
A5UK11	Methanobrevibacter smithii (strain ATCC 35061 / DSM 861 / OCM 144 / PS)	27%	89%
A6VGK5	Methanococcus maripaludis (strain C7 / ATCC BAA-1331)	26%	93%
A9AA46	Methanococcus maripaludis (strain C6 / ATCC BAA-1332)	26%	93%
C3MPS1	Sulfolobus islandicus (strain L.S.2.15 / Lassen #1)	27%	88%
C3MYR5	Sulfolobus islandicus (strain M.14.25 / Kamchatka #1)	27%	88%
C3N5E7	Sulfolobus islandicus (strain M.16.27)	27%	88%
C3NE01	Sulfolobus islandicus (strain Y.G.57.14 / Yellowstone #1)	27%	88%
C3NHQ2	Sulfolobus islandicus (strain Y.N.15.51 / Yellowstone #2)	27%	88%
C4KH13	Sulfolobus islandicus (strain M.16.4 / Kamchatka #3)	27%	88%
E9AQ41	Leishmania mexicana (strain MHOM/GT/2001/U1103)	83%	98%
E9ATM7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	30%	100%
O26802	Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)	25%	90%
O59132	Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)	29%	89%
O88202	Rattus norvegicus	31%	69%
P0A962	Escherichia coli (strain K12)	38%	100%
P0A963	Escherichia coli O157:H7	38%	100%
P61400	Methanococcus maripaludis (strain S2 / LL)	26%	93%
Q4Q0X1	Leishmania major	29%	100%
Q4QFE1	Leishmania major	80%	100%
Q5JI77	Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)	26%	89%
Q60331	Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)	24%	94%
Q86U10	Homo sapiens	30%	68%
Q8TV84	Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)	26%	85%
Q8TZE8	Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)	25%	100%
Q8U0X0	Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)	27%	89%
Q97ZH5	Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)	27%	88%
Q9HJJ5	Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)	28%	95%
Q9U518	Dirofilaria immitis	31%	66%
Q9V0T9	Pyrococcus abyssi (strain GE5 / Orsay)	28%	89%
V5BC16	Trypanosoma cruzi	52%	76%
V5BUU3	Trypanosoma cruzi	29%	100%