Putative replication Factor A 28 kDa subunit

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

replication Factor A 28 kDa subunit, putative

Species:

Leishmania braziliensis

UniProt:

A4H800_LEIBR

TriTrypDb:

LbrM.15.0300 , LBRM2903_150008000

Length:

183

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 25

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 6
NetGPI	no	yes: 0, no: 6

Cellular components
Term	Name	Level	Count
GO:0000781	chromosome, telomeric region	3	1
GO:0005634	nucleus	5	5
GO:0005654	nucleoplasm	2	1
GO:0005662	DNA replication factor A complex	3	1
GO:0032991	protein-containing complex	1	1
GO:0035861	site of double-strand break	3	1
GO:0043226	organelle	2	5
GO:0043227	membrane-bounded organelle	3	5
GO:0043229	intracellular organelle	3	5
GO:0043231	intracellular membrane-bounded organelle	4	5
GO:0090734	site of DNA damage	2	1
GO:0098687	chromosomal region	2	1
GO:0110165	cellular anatomical entity	1	5
GO:0140513	nuclear protein-containing complex	2	1

Expansion

Sequence features

A4H800

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H800

Function

Biological processes
Term	Name	Level	Count
GO:0000724	double-strand break repair via homologous recombination	7	1
GO:0000725	recombinational repair	6	1
GO:0006139	nucleobase-containing compound metabolic process	3	5
GO:0006259	DNA metabolic process	4	5
GO:0006260	DNA replication	5	5
GO:0006281	DNA repair	5	5
GO:0006289	nucleotide-excision repair	6	1
GO:0006302	double-strand break repair	6	1
GO:0006310	DNA recombination	5	5
GO:0006725	cellular aromatic compound metabolic process	3	5
GO:0006807	nitrogen compound metabolic process	2	5
GO:0006950	response to stress	2	5
GO:0006974	DNA damage response	4	5
GO:0008152	metabolic process	1	5
GO:0009987	cellular process	1	5
GO:0033554	cellular response to stress	3	5
GO:0034641	cellular nitrogen compound metabolic process	3	5
GO:0043170	macromolecule metabolic process	3	5
GO:0044237	cellular metabolic process	2	5
GO:0044238	primary metabolic process	2	5
GO:0044260	obsolete cellular macromolecule metabolic process	3	5
GO:0046483	heterocycle metabolic process	3	5
GO:0050896	response to stimulus	1	5
GO:0051716	cellular response to stimulus	2	5
GO:0071704	organic substance metabolic process	2	5
GO:0090304	nucleic acid metabolic process	4	5
GO:1901360	organic cyclic compound metabolic process	3	5

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	7
GO:0005488	binding	1	7
GO:0097159	organic cyclic compound binding	2	7
GO:1901363	heterocyclic compound binding	2	7
GO:0003677	DNA binding	4	5
GO:0003697	single-stranded DNA binding	5	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	66	70	PF00656	0.562
CLV_PCSK_KEX2_1	92	94	PF00082	0.386
CLV_PCSK_PC1ET2_1	92	94	PF00082	0.436
CLV_PCSK_SKI1_1	120	124	PF00082	0.436
DOC_CKS1_1	55	60	PF01111	0.560
DOC_USP7_MATH_1	139	143	PF00917	0.436
DOC_WW_Pin1_4	54	59	PF00397	0.560
LIG_14-3-3_CanoR_1	152	160	PF00244	0.436
LIG_14-3-3_CanoR_1	16	24	PF00244	0.570
LIG_BIR_II_1	1	5	PF00653	0.518
LIG_FHA_1	122	128	PF00498	0.436
LIG_FHA_1	19	25	PF00498	0.540
LIG_FHA_1	48	54	PF00498	0.570
LIG_FHA_1	55	61	PF00498	0.570
LIG_FHA_2	154	160	PF00498	0.436
LIG_LIR_Gen_1	72	82	PF02991	0.542
LIG_LIR_LC3C_4	21	26	PF02991	0.570
LIG_LIR_Nem_3	72	78	PF02991	0.545
LIG_NRBOX	122	128	PF00104	0.436
LIG_PDZ_Class_2	178	183	PF00595	0.571
LIG_SH3_3	32	38	PF00018	0.462
LIG_WRC_WIRS_1	127	132	PF05994	0.436
MOD_CK1_1	97	103	PF00069	0.448
MOD_CK2_1	139	145	PF00069	0.436
MOD_GlcNHglycan	105	108	PF01048	0.444
MOD_GlcNHglycan	135	138	PF01048	0.500
MOD_GlcNHglycan	2	5	PF01048	0.627
MOD_GlcNHglycan	47	50	PF01048	0.370
MOD_GlcNHglycan	96	99	PF01048	0.440
MOD_GSK3_1	159	166	PF00069	0.436
MOD_GSK3_1	54	61	PF00069	0.592
MOD_GSK3_1	63	70	PF00069	0.635
MOD_GSK3_1	97	104	PF00069	0.374
MOD_NEK2_1	126	131	PF00069	0.436
MOD_NEK2_1	85	90	PF00069	0.436
MOD_PIKK_1	85	91	PF00454	0.436
MOD_PKA_2	15	21	PF00069	0.570
MOD_PKA_2	151	157	PF00069	0.314
MOD_PKA_2	45	51	PF00069	0.570
MOD_PKA_2	85	91	PF00069	0.436
MOD_Plk_1	144	150	PF00069	0.347
MOD_Plk_4	126	132	PF00069	0.436
MOD_Plk_4	60	66	PF00069	0.570
MOD_ProDKin_1	54	60	PF00069	0.560
TRG_ENDOCYTIC_2	75	78	PF00928	0.551
TRG_Pf-PMV_PEXEL_1	165	170	PF00026	0.377

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I2B8	Leptomonas seymouri	90%	72%
A0A0S4JPP2	Bodo saltans	33%	100%
A0A1X0NTN7	Trypanosomatidae	61%	71%
A0A3Q8IDB6	Leishmania donovani	92%	71%
A0A3S5IRR4	Trypanosoma rangeli	61%	68%
A4HWC8	Leishmania infantum	92%	71%
C9ZNX0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	56%	100%
E9AQ28	Leishmania mexicana (strain MHOM/GT/2001/U1103)	93%	71%
Q23697	Crithidia fasciculata	90%	71%
Q4QFF3	Leishmania major	92%	100%
Q5RC43	Pongo abelii	26%	68%