Poly(A) polymerase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Poly(A) polymerase

Gene product:

polynucleotide adenylyltransferase, putative

Species:

Leishmania braziliensis

UniProt:

A4H7T8_LEIBR

TriTrypDb:

LbrM.14.1350 , LBRM2903_140018200

Length:

709

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	12
GO:0005737	cytoplasm	2	12
GO:0043226	organelle	2	12
GO:0043227	membrane-bounded organelle	3	12
GO:0043229	intracellular organelle	3	12
GO:0043231	intracellular membrane-bounded organelle	4	12
GO:0110165	cellular anatomical entity	1	12

Expansion

Sequence features

A4H7T8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H7T8

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006396	RNA processing	6	12
GO:0006397	mRNA processing	7	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0016071	mRNA metabolic process	6	12
GO:0031123	RNA 3'-end processing	7	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043631	RNA polyadenylation	6	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071076	RNA 3' uridylation	8	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:0006378	mRNA polyadenylation	7	1
GO:0031124	mRNA 3'-end processing	8	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	12
GO:0003676	nucleic acid binding	3	12
GO:0003723	RNA binding	4	12
GO:0003824	catalytic activity	1	12
GO:0004652	obsolete polynucleotide adenylyltransferase activity	6	12
GO:0005488	binding	1	12
GO:0005524	ATP binding	5	12
GO:0016740	transferase activity	2	12
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	12
GO:0016779	nucleotidyltransferase activity	4	12
GO:0017076	purine nucleotide binding	4	12
GO:0030554	adenyl nucleotide binding	5	12
GO:0032553	ribonucleotide binding	3	12
GO:0032555	purine ribonucleotide binding	4	12
GO:0032559	adenyl ribonucleotide binding	5	12
GO:0035639	purine ribonucleoside triphosphate binding	4	12
GO:0036094	small molecule binding	2	12
GO:0043167	ion binding	2	12
GO:0043168	anion binding	3	12
GO:0043169	cation binding	3	12
GO:0046872	metal ion binding	4	12
GO:0050265	RNA uridylyltransferase activity	4	12
GO:0070566	adenylyltransferase activity	5	12
GO:0070569	uridylyltransferase activity	5	12
GO:0097159	organic cyclic compound binding	2	12
GO:0097367	carbohydrate derivative binding	2	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:1901265	nucleoside phosphate binding	3	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	302	306	PF00656	0.315
CLV_C14_Caspase3-7	388	392	PF00656	0.294
CLV_C14_Caspase3-7	615	619	PF00656	0.629
CLV_C14_Caspase3-7	649	653	PF00656	0.758
CLV_C14_Caspase3-7	663	667	PF00656	0.526
CLV_C14_Caspase3-7	680	684	PF00656	0.422
CLV_NRD_NRD_1	434	436	PF00675	0.346
CLV_NRD_NRD_1	50	52	PF00675	0.306
CLV_PCSK_KEX2_1	434	436	PF00082	0.321
CLV_PCSK_KEX2_1	49	51	PF00082	0.306
CLV_PCSK_KEX2_1	598	600	PF00082	0.709
CLV_PCSK_KEX2_1	657	659	PF00082	0.713
CLV_PCSK_PC1ET2_1	598	600	PF00082	0.709
CLV_PCSK_PC1ET2_1	657	659	PF00082	0.713
CLV_PCSK_SKI1_1	204	208	PF00082	0.311
CLV_PCSK_SKI1_1	212	216	PF00082	0.281
CLV_PCSK_SKI1_1	51	55	PF00082	0.299
CLV_PCSK_SKI1_1	595	599	PF00082	0.501
CLV_PCSK_SKI1_1	9	13	PF00082	0.483
DEG_APCC_DBOX_1	207	215	PF00400	0.299
DEG_APCC_DBOX_1	50	58	PF00400	0.299
DEG_SCF_TRCP1_1	683	689	PF00400	0.506
DOC_CKS1_1	312	317	PF01111	0.299
DOC_MAPK_DCC_7	140	149	PF00069	0.315
DOC_MAPK_FxFP_2	228	231	PF00069	0.315
DOC_MAPK_gen_1	300	309	PF00069	0.315
DOC_MAPK_gen_1	378	387	PF00069	0.193
DOC_MAPK_HePTP_8	53	65	PF00069	0.419
DOC_MAPK_MEF2A_6	140	149	PF00069	0.315
DOC_MAPK_MEF2A_6	378	387	PF00069	0.322
DOC_MAPK_MEF2A_6	56	65	PF00069	0.402
DOC_PP1_RVXF_1	405	411	PF00149	0.322
DOC_PP2B_LxvP_1	108	111	PF13499	0.446
DOC_PP2B_PxIxI_1	543	549	PF00149	0.315
DOC_PP4_FxxP_1	228	231	PF00568	0.315
DOC_PP4_FxxP_1	436	439	PF00568	0.384
DOC_PP4_FxxP_1	73	76	PF00568	0.315
DOC_USP7_MATH_1	299	303	PF00917	0.315
DOC_USP7_MATH_1	574	578	PF00917	0.699
DOC_USP7_MATH_1	616	620	PF00917	0.749
DOC_USP7_MATH_1	634	638	PF00917	0.673
DOC_USP7_MATH_1	675	679	PF00917	0.733
DOC_USP7_MATH_1	681	685	PF00917	0.724
DOC_USP7_MATH_1	89	93	PF00917	0.458
DOC_USP7_MATH_2	299	305	PF00917	0.315
DOC_USP7_UBL2_3	215	219	PF12436	0.299
DOC_WW_Pin1_4	311	316	PF00397	0.299
DOC_WW_Pin1_4	480	485	PF00397	0.380
LIG_14-3-3_CanoR_1	258	264	PF00244	0.343
LIG_14-3-3_CanoR_1	288	297	PF00244	0.419
LIG_14-3-3_CanoR_1	417	422	PF00244	0.356
LIG_14-3-3_CanoR_1	49	54	PF00244	0.313
LIG_14-3-3_CanoR_1	629	638	PF00244	0.651
LIG_14-3-3_CanoR_1	66	70	PF00244	0.240
LIG_14-3-3_CanoR_1	668	672	PF00244	0.518
LIG_BIR_II_1	1	5	PF00653	0.549
LIG_BIR_III_3	1	5	PF00653	0.465
LIG_BRCT_BRCA1_1	224	228	PF00533	0.315
LIG_EH1_1	194	202	PF00400	0.334
LIG_FHA_1	171	177	PF00498	0.299
LIG_FHA_1	238	244	PF00498	0.419
LIG_FHA_1	312	318	PF00498	0.310
LIG_FHA_1	468	474	PF00498	0.454
LIG_FHA_1	488	494	PF00498	0.193
LIG_FHA_1	621	627	PF00498	0.661
LIG_FHA_2	272	278	PF00498	0.384
LIG_FHA_2	37	43	PF00498	0.395
LIG_FHA_2	418	424	PF00498	0.394
LIG_FHA_2	5	11	PF00498	0.600
LIG_FHA_2	545	551	PF00498	0.300
LIG_FHA_2	613	619	PF00498	0.750
LIG_FHA_2	678	684	PF00498	0.624
LIG_LIR_Apic_2	225	231	PF02991	0.315
LIG_LIR_Apic_2	265	269	PF02991	0.334
LIG_LIR_Apic_2	72	76	PF02991	0.315
LIG_LIR_Gen_1	117	127	PF02991	0.446
LIG_LIR_Gen_1	525	533	PF02991	0.384
LIG_LIR_Nem_3	117	123	PF02991	0.446
LIG_LIR_Nem_3	157	163	PF02991	0.299
LIG_LIR_Nem_3	257	263	PF02991	0.419
LIG_LIR_Nem_3	357	363	PF02991	0.402
LIG_LIR_Nem_3	393	398	PF02991	0.339
LIG_LIR_Nem_3	422	427	PF02991	0.356
LIG_LIR_Nem_3	525	529	PF02991	0.384
LIG_MYND_1	311	315	PF01753	0.315
LIG_PDZ_Class_2	704	709	PF00595	0.543
LIG_Pex14_2	213	217	PF04695	0.299
LIG_Pex14_2	375	379	PF04695	0.446
LIG_PTB_Apo_2	318	325	PF02174	0.299
LIG_PTB_Phospho_1	318	324	PF10480	0.334
LIG_REV1ctd_RIR_1	257	265	PF16727	0.294
LIG_REV1ctd_RIR_1	373	382	PF16727	0.419
LIG_SH2_CRK	424	428	PF00017	0.419
LIG_SH2_GRB2like	224	227	PF00017	0.315
LIG_SH2_SRC	382	385	PF00017	0.234
LIG_SH2_SRC	398	401	PF00017	0.410
LIG_SH2_STAP1	224	228	PF00017	0.315
LIG_SH2_STAT3	247	250	PF00017	0.419
LIG_SH2_STAT5	282	285	PF00017	0.315
LIG_SH2_STAT5	382	385	PF00017	0.299
LIG_SH2_STAT5	502	505	PF00017	0.302
LIG_SH2_STAT5	564	567	PF00017	0.530
LIG_SH3_3	309	315	PF00018	0.299
LIG_SH3_3	366	372	PF00018	0.472
LIG_SUMO_SIM_par_1	544	550	PF11976	0.284
LIG_TRAF2_1	43	46	PF00917	0.378
LIG_UBA3_1	197	204	PF00899	0.315
LIG_WRC_WIRS_1	523	528	PF05994	0.384
LIG_WRC_WIRS_1	554	559	PF05994	0.419
MOD_CDK_SPxxK_3	480	487	PF00069	0.193
MOD_CK1_1	112	118	PF00069	0.532
MOD_CK1_1	159	165	PF00069	0.293
MOD_CK1_1	184	190	PF00069	0.299
MOD_CK1_1	222	228	PF00069	0.446
MOD_CK1_1	450	456	PF00069	0.341
MOD_CK1_1	464	470	PF00069	0.240
MOD_CK1_1	476	482	PF00069	0.251
MOD_CK1_1	489	495	PF00069	0.374
MOD_CK1_1	587	593	PF00069	0.729
MOD_CK1_1	609	615	PF00069	0.715
MOD_CK1_1	619	625	PF00069	0.720
MOD_CK1_1	642	648	PF00069	0.644
MOD_CK2_1	271	277	PF00069	0.334
MOD_CK2_1	29	35	PF00069	0.402
MOD_CK2_1	349	355	PF00069	0.441
MOD_CK2_1	36	42	PF00069	0.439
MOD_CK2_1	387	393	PF00069	0.299
MOD_CK2_1	49	55	PF00069	0.115
MOD_CK2_1	575	581	PF00069	0.693
MOD_CK2_1	627	633	PF00069	0.722
MOD_GlcNHglycan	17	20	PF01048	0.414
MOD_GlcNHglycan	2	5	PF01048	0.546
MOD_GlcNHglycan	231	234	PF01048	0.299
MOD_GlcNHglycan	351	354	PF01048	0.496
MOD_GlcNHglycan	452	455	PF01048	0.251
MOD_GlcNHglycan	475	478	PF01048	0.363
MOD_GlcNHglycan	513	516	PF01048	0.335
MOD_GlcNHglycan	592	595	PF01048	0.644
MOD_GlcNHglycan	640	644	PF01048	0.661
MOD_GlcNHglycan	683	686	PF01048	0.706
MOD_GlcNHglycan	99	102	PF01048	0.246
MOD_GSK3_1	413	420	PF00069	0.341
MOD_GSK3_1	443	450	PF00069	0.366
MOD_GSK3_1	467	474	PF00069	0.299
MOD_GSK3_1	476	483	PF00069	0.360
MOD_GSK3_1	489	496	PF00069	0.408
MOD_GSK3_1	507	514	PF00069	0.402
MOD_GSK3_1	549	556	PF00069	0.426
MOD_GSK3_1	583	590	PF00069	0.610
MOD_GSK3_1	600	607	PF00069	0.642
MOD_GSK3_1	608	615	PF00069	0.661
MOD_GSK3_1	616	623	PF00069	0.769
MOD_GSK3_1	642	649	PF00069	0.628
MOD_GSK3_1	677	684	PF00069	0.668
MOD_N-GLC_1	324	329	PF02516	0.299
MOD_N-GLC_1	412	417	PF02516	0.419
MOD_N-GLC_1	458	463	PF02516	0.296
MOD_N-GLC_1	584	589	PF02516	0.749
MOD_N-GLC_1	689	694	PF02516	0.673
MOD_N-GLC_2	321	323	PF02516	0.299
MOD_NEK2_1	119	124	PF00069	0.446
MOD_NEK2_1	259	264	PF00069	0.419
MOD_NEK2_1	283	288	PF00069	0.299
MOD_NEK2_1	349	354	PF00069	0.550
MOD_NEK2_1	473	478	PF00069	0.368
MOD_NEK2_1	486	491	PF00069	0.216
MOD_NEK2_1	493	498	PF00069	0.166
MOD_NEK2_1	604	609	PF00069	0.663
MOD_NEK2_2	319	324	PF00069	0.446
MOD_NEK2_2	522	527	PF00069	0.446
MOD_NEK2_2	634	639	PF00069	0.499
MOD_PK_1	219	225	PF00069	0.446
MOD_PK_1	49	55	PF00069	0.318
MOD_PKA_1	49	55	PF00069	0.334
MOD_PKA_2	112	118	PF00069	0.446
MOD_PKA_2	139	145	PF00069	0.356
MOD_PKA_2	299	305	PF00069	0.315
MOD_PKA_2	461	467	PF00069	0.348
MOD_PKA_2	49	55	PF00069	0.300
MOD_PKA_2	583	589	PF00069	0.595
MOD_PKA_2	65	71	PF00069	0.254
MOD_PKA_2	667	673	PF00069	0.765
MOD_PKB_1	445	453	PF00069	0.419
MOD_Plk_1	156	162	PF00069	0.299
MOD_Plk_1	181	187	PF00069	0.300
MOD_Plk_1	29	35	PF00069	0.447
MOD_Plk_1	324	330	PF00069	0.315
MOD_Plk_1	487	493	PF00069	0.193
MOD_Plk_1	549	555	PF00069	0.205
MOD_Plk_1	627	633	PF00069	0.643
MOD_Plk_1	675	681	PF00069	0.749
MOD_Plk_1	689	695	PF00069	0.601
MOD_Plk_2-3	170	176	PF00069	0.299
MOD_Plk_4	181	187	PF00069	0.310
MOD_Plk_4	262	268	PF00069	0.334
MOD_Plk_4	493	499	PF00069	0.419
MOD_Plk_4	553	559	PF00069	0.406
MOD_Plk_4	634	640	PF00069	0.500
MOD_Plk_4	65	71	PF00069	0.299
MOD_ProDKin_1	311	317	PF00069	0.299
MOD_ProDKin_1	480	486	PF00069	0.380
MOD_SUMO_for_1	127	130	PF00179	0.532
MOD_SUMO_for_1	673	676	PF00179	0.741
MOD_SUMO_rev_2	298	304	PF00179	0.316
MOD_SUMO_rev_2	352	360	PF00179	0.410
TRG_ENDOCYTIC_2	382	385	PF00928	0.306
TRG_ENDOCYTIC_2	424	427	PF00928	0.419
TRG_ER_diArg_1	287	290	PF00400	0.446
TRG_ER_diArg_1	405	408	PF00400	0.419
TRG_ER_diArg_1	433	435	PF00400	0.332
TRG_ER_diArg_1	444	447	PF00400	0.294
TRG_ER_diArg_1	49	51	PF00400	0.315
TRG_Pf-PMV_PEXEL_1	332	336	PF00026	0.293
TRG_Pf-PMV_PEXEL_1	531	535	PF00026	0.315
TRG_Pf-PMV_PEXEL_1	691	696	PF00026	0.495

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I2K1	Leptomonas seymouri	71%	96%
A0A0S4JIG5	Bodo saltans	47%	100%
A0A1X0NP17	Trypanosomatidae	60%	100%
A0A3Q8ID77	Leishmania donovani	86%	100%
A0A3R7KE83	Trypanosoma rangeli	63%	100%
A4HW64	Leishmania infantum	86%	100%
A4I4T6	Leishmania infantum	27%	100%
C9ZSW0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	57%	100%
E9AEC2	Leishmania major	27%	100%
E9ALG4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	26%	100%
E9APW5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	87%	100%
O42617	Candida albicans (strain SC5314 / ATCC MYA-2876)	29%	100%
Q4QFL6	Leishmania major	85%	99%
V5BKK6	Trypanosoma cruzi	59%	87%