Putative calpain-like cysteine peptidase

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

cysteine peptidase, Clan CA, family C2, putative

Species:

Leishmania braziliensis

UniProt:

A4H7N9_LEIBR

TriTrypDb:

LbrM.14.0820 , LBRM2903_140013900

Length:

115

Annotations

LeishMANIAdb interaction annotations

by homology
Contact email: avilan@ula.ve
Publication title: Plasminogen binding proteins in secreted membrane vesicles of Leishmania mexicana
Publication 1st author(s): Figuera
Publication Identifier(s): 23178693
Host organism: -1
Interaction detection method(s): enzymatic linked imunosorbent assay
Interaction type: direct interaction
Identification method participant A: identification by antibody
Identification method participant B: identification by antibody
ID(s) interactor A: E9AUS1
ID(s) interactor B: P00747
Taxid interactor A: Leishmania mexicana
Taxid interactor B: Homo sapiens
Biological role(s) interactor A: unspecified role
Biological role(s) interactor B: unspecified role
Experimental role(s) interactor A: bait

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 2
Forrest at al. (metacyclic)	no	yes: 6
Forrest at al. (procyclic)	no	yes: 8
Silverman et al.	no	yes: 1
Pissara et al.	yes	yes: 31

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 15

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 30

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 60
NetGPI	no	yes: 0, no: 60

Cellular components
Term	Name	Level	Count
GO:0005654	nucleoplasm	2	1
GO:0005737	cytoplasm	2	1
GO:0031974	membrane-enclosed lumen	2	1
GO:0031981	nuclear lumen	5	1
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	1
GO:0043233	organelle lumen	3	1
GO:0070013	intracellular organelle lumen	4	1
GO:0097014	ciliary plasm	5	1
GO:0097542	ciliary tip	2	1
GO:0099568	cytoplasmic region	3	1
GO:0110165	cellular anatomical entity	1	3
GO:0016020	membrane	2	2
GO:0020018	ciliary pocket membrane	6	1
GO:0031090	organelle membrane	3	2
GO:0031253	cell projection membrane	4	2
GO:0060170	ciliary membrane	5	2
GO:0098588	bounding membrane of organelle	4	2
GO:0098590	plasma membrane region	3	2

Expansion

Sequence features

A4H7N9

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H7N9

PDB structure(s): 1r75_A , 2fe0_A

Function

Biological processes
Term	Name	Level	Count
GO:0001539	cilium or flagellum-dependent cell motility	3	1
GO:0009987	cellular process	1	2
GO:0048870	cell motility	2	1
GO:0060285	cilium-dependent cell motility	4	1
GO:0006996	organelle organization	4	1
GO:0016043	cellular component organization	3	1
GO:0022607	cellular component assembly	4	1
GO:0030030	cell projection organization	4	1
GO:0030031	cell projection assembly	5	1
GO:0044000	movement in host	4	1
GO:0044403	biological process involved in symbiotic interaction	2	1
GO:0044409	entry into host	5	1
GO:0044419	biological process involved in interspecies interaction between organisms	1	1
GO:0044782	cilium organization	5	1
GO:0051701	biological process involved in interaction with host	3	1
GO:0060271	cilium assembly	6	1
GO:0070925	organelle assembly	5	1
GO:0071840	cellular component organization or biogenesis	2	1
GO:0120031	plasma membrane bounded cell projection assembly	6	1
GO:0120036	plasma membrane bounded cell projection organization	5	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	1
GO:0016787	hydrolase activity	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_PCSK_SKI1_1	20	24	PF00082	0.407
CLV_PCSK_SKI1_1	40	44	PF00082	0.464
CLV_PCSK_SKI1_1	55	59	PF00082	0.466
DEG_Kelch_Keap1_1	75	80	PF01344	0.458
DOC_PP1_RVXF_1	102	108	PF00149	0.458
DOC_PP1_RVXF_1	53	59	PF00149	0.386
LIG_BIR_II_1	1	5	PF00653	0.683
LIG_BRCT_BRCA1_1	77	81	PF00533	0.281
LIG_deltaCOP1_diTrp_1	36	43	PF00928	0.503
LIG_LIR_Gen_1	47	58	PF02991	0.409
LIG_LIR_Nem_3	38	44	PF02991	0.413
LIG_LIR_Nem_3	47	53	PF02991	0.405
LIG_Pex14_2	103	107	PF04695	0.500
LIG_SH2_CRK	50	54	PF00017	0.414
LIG_SH2_GRB2like	6	9	PF00017	0.507
LIG_SH2_NCK_1	6	10	PF00017	0.241
LIG_SH2_SRC	6	9	PF00017	0.500
LIG_TYR_ITSM	46	53	PF00017	0.481
MOD_CK1_1	14	20	PF00069	0.516
MOD_CK2_1	59	65	PF00069	0.398
MOD_GlcNHglycan	13	16	PF01048	0.505
MOD_GlcNHglycan	75	80	PF01048	0.360
MOD_N-GLC_1	44	49	PF02516	0.369
MOD_N-GLC_1	68	73	PF02516	0.507
MOD_Plk_1	68	74	PF00069	0.434
MOD_Plk_4	14	20	PF00069	0.405
MOD_SUMO_for_1	4	7	PF00179	0.623
MOD_SUMO_for_1	81	84	PF00179	0.435
MOD_SUMO_rev_2	36	42	PF00179	0.503
TRG_ENDOCYTIC_2	50	53	PF00928	0.421
TRG_NES_CRM1_1	86	99	PF08389	0.415

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P6Y3	Leptomonas seymouri	43%	87%
A0A0N0P7C3	Leptomonas seymouri	33%	79%
A0A0N1HYV4	Leptomonas seymouri	39%	96%
A0A0N1I5S6	Leptomonas seymouri	40%	75%
A0A0N1I8U4	Leptomonas seymouri	33%	85%
A0A0N1PCZ0	Leptomonas seymouri	80%	88%
A0A0N1PE11	Leptomonas seymouri	36%	81%
A0A1X0NNW3	Trypanosomatidae	57%	100%
A0A1X0NW95	Trypanosomatidae	50%	88%
A0A1X0NWH2	Trypanosomatidae	37%	82%
A0A1X0NWV3	Trypanosomatidae	50%	77%
A0A1X0NXS9	Trypanosomatidae	38%	85%
A0A1X0NXT9	Trypanosomatidae	36%	89%
A0A1X0NYV3	Trypanosomatidae	38%	83%
A0A3Q8I9V9	Leishmania donovani	91%	100%
A0A3Q8IBM6	Leishmania donovani	32%	86%
A0A3Q8IBP3	Leishmania donovani	33%	79%
A0A3Q8IDP8	Leishmania donovani	44%	76%
A0A3Q8IKN9	Leishmania donovani	45%	88%
A0A3R7MB04	Trypanosoma rangeli	68%	100%
A0A3R7MWC6	Trypanosoma rangeli	50%	86%
A0A3S7WW23	Leishmania donovani	42%	85%
A0A3S7WZ86	Leishmania donovani	36%	81%
A0A422MSY8	Trypanosoma rangeli	50%	68%
A0A422MSZ3	Trypanosoma rangeli	51%	100%
A0A422MUY0	Trypanosoma rangeli	39%	77%
A0A422N035	Trypanosoma rangeli	30%	82%
A0A422N058	Trypanosoma rangeli	39%	75%
A4H7P0	Leishmania braziliensis	99%	100%
A4HEG7	Leishmania braziliensis	37%	83%
A4HW29	Leishmania infantum	91%	100%
A4HYW5	Leishmania infantum	33%	79%
A4HYW6	Leishmania infantum	32%	86%
A4HYX1	Leishmania infantum	44%	76%
A4HYX3	Leishmania infantum	42%	85%
A4HYX4	Leishmania infantum	45%	88%
A4I1M3	Leishmania infantum	36%	81%
C9ZIF2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	37%	88%
C9ZIF5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	44%	93%
C9ZIF6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	43%	89%
E9AIH7	Leishmania braziliensis	33%	79%
E9AIH8	Leishmania braziliensis	31%	86%
E9AII3	Leishmania braziliensis	41%	76%
E9AII5	Leishmania braziliensis	44%	85%
E9AII6	Leishmania braziliensis	44%	88%
E9APT0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	91%	100%
E9AUR2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	33%	79%
E9AUR3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	32%	86%
E9AUR8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	45%	76%
E9AUS0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	44%	85%
E9AUS1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	46%	88%
E9AXQ7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	35%	81%
P84150	Leishmania major	32%	100%
Q4Q9R4	Leishmania major	35%	100%
Q4QCS4	Leishmania major	33%	79%
Q4QFP8	Leishmania major	91%	100%
Q5SDH3	Leishmania major	37%	100%
Q5SDH4	Leishmania major	43%	100%
Q5SDH5	Leishmania major	45%	100%