Putative ras-like small GTPases

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ras-like small GTPases, putative

Species:

Leishmania braziliensis

UniProt:

A4H7E6_LEIBR

TriTrypDb:

LbrM.13.1420 , LBRM2903_130020200

Length:

353

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0000323	lytic vacuole	6	1
GO:0005634	nucleus	5	1
GO:0005764	lysosome	7	1
GO:0005773	vacuole	5	1
GO:0032991	protein-containing complex	1	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1
GO:1902494	catalytic complex	2	1
GO:1905360	GTPase complex	3	1
GO:1990131	Gtr1-Gtr2 GTPase complex	4	1

Expansion

Sequence features

A4H7E6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H7E6

Function

Biological processes
Term	Name	Level	Count
GO:0001101	response to acid chemical	3	1
GO:0006950	response to stress	2	1
GO:0007154	cell communication	2	1
GO:0009267	cellular response to starvation	4	1
GO:0009605	response to external stimulus	2	1
GO:0009719	response to endogenous stimulus	2	1
GO:0009894	regulation of catabolic process	4	1
GO:0009966	regulation of signal transduction	4	1
GO:0009967	positive regulation of signal transduction	5	1
GO:0009987	cellular process	1	1
GO:0009991	response to extracellular stimulus	3	1
GO:0010033	response to organic substance	3	1
GO:0010243	response to organonitrogen compound	4	1
GO:0010506	regulation of autophagy	6	1
GO:0010646	regulation of cell communication	4	1
GO:0010647	positive regulation of cell communication	5	1
GO:0019222	regulation of metabolic process	3	1
GO:0023051	regulation of signaling	3	1
GO:0023056	positive regulation of signaling	4	1
GO:0031323	regulation of cellular metabolic process	4	1
GO:0031329	regulation of cellular catabolic process	5	1
GO:0031667	response to nutrient levels	4	1
GO:0031668	cellular response to extracellular stimulus	3	1
GO:0031669	cellular response to nutrient levels	4	1
GO:0032006	regulation of TOR signaling	6	1
GO:0032008	positive regulation of TOR signaling	7	1
GO:0033554	cellular response to stress	3	1
GO:0042221	response to chemical	2	1
GO:0042594	response to starvation	3	1
GO:0043200	response to amino acid	4	1
GO:0048518	positive regulation of biological process	3	1
GO:0048522	positive regulation of cellular process	4	1
GO:0048583	regulation of response to stimulus	3	1
GO:0048584	positive regulation of response to stimulus	4	1
GO:0050789	regulation of biological process	2	1
GO:0050794	regulation of cellular process	3	1
GO:0050896	response to stimulus	1	1
GO:0051716	cellular response to stimulus	2	1
GO:0065007	biological regulation	1	1
GO:0070887	cellular response to chemical stimulus	3	1
GO:0071229	cellular response to acid chemical	4	1
GO:0071230	cellular response to amino acid stimulus	5	1
GO:0071310	cellular response to organic substance	4	1
GO:0071417	cellular response to organonitrogen compound	4	1
GO:0071495	cellular response to endogenous stimulus	3	1
GO:0071496	cellular response to external stimulus	3	1
GO:1901698	response to nitrogen compound	3	1
GO:1901699	cellular response to nitrogen compound	4	1
GO:1901700	response to oxygen-containing compound	3	1
GO:1901701	cellular response to oxygen-containing compound	4	1
GO:1902531	regulation of intracellular signal transduction	5	1
GO:1902533	positive regulation of intracellular signal transduction	6	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	12
GO:0005488	binding	1	12
GO:0005525	GTP binding	5	12
GO:0017076	purine nucleotide binding	4	12
GO:0019001	guanyl nucleotide binding	5	12
GO:0032553	ribonucleotide binding	3	12
GO:0032555	purine ribonucleotide binding	4	12
GO:0032561	guanyl ribonucleotide binding	5	12
GO:0035639	purine ribonucleoside triphosphate binding	4	12
GO:0036094	small molecule binding	2	12
GO:0043167	ion binding	2	12
GO:0043168	anion binding	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:0097367	carbohydrate derivative binding	2	12
GO:1901265	nucleoside phosphate binding	3	12
GO:1901363	heterocyclic compound binding	2	12
GO:0003824	catalytic activity	1	1
GO:0003924	GTPase activity	7	1
GO:0016462	pyrophosphatase activity	5	1
GO:0016787	hydrolase activity	2	1
GO:0016817	hydrolase activity, acting on acid anhydrides	3	1
GO:0016818	hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	4	1
GO:0017111	ribonucleoside triphosphate phosphatase activity	6	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	337	341	PF00656	0.260
CLV_NRD_NRD_1	167	169	PF00675	0.247
CLV_NRD_NRD_1	333	335	PF00675	0.486
CLV_PCSK_KEX2_1	332	334	PF00082	0.490
CLV_PCSK_SKI1_1	211	215	PF00082	0.278
CLV_PCSK_SKI1_1	246	250	PF00082	0.232
CLV_PCSK_SKI1_1	274	278	PF00082	0.323
CLV_PCSK_SKI1_1	295	299	PF00082	0.365
CLV_PCSK_SKI1_1	33	37	PF00082	0.256
CLV_PCSK_SKI1_1	53	57	PF00082	0.112
CLV_PCSK_SKI1_1	67	71	PF00082	0.384
CLV_PCSK_SKI1_1	8	12	PF00082	0.241
DEG_Nend_UBRbox_2	1	3	PF02207	0.516
DOC_CYCLIN_RxL_1	50	61	PF00134	0.384
DOC_CYCLIN_yClb5_NLxxxL_5	53	59	PF00134	0.384
DOC_MAPK_gen_1	143	150	PF00069	0.317
DOC_MAPK_MEF2A_6	143	150	PF00069	0.317
DOC_MAPK_MEF2A_6	33	42	PF00069	0.241
DOC_MAPK_MEF2A_6	82	90	PF00069	0.241
DOC_MAPK_NFAT4_5	33	41	PF00069	0.241
DOC_PP1_RVXF_1	21	28	PF00149	0.241
DOC_USP7_MATH_1	199	203	PF00917	0.339
DOC_USP7_MATH_1	250	254	PF00917	0.278
DOC_USP7_UBL2_3	4	8	PF12436	0.525
DOC_WW_Pin1_4	138	143	PF00397	0.280
LIG_14-3-3_CanoR_1	235	239	PF00244	0.275
LIG_14-3-3_CanoR_1	265	273	PF00244	0.256
LIG_Actin_WH2_2	24	39	PF00022	0.241
LIG_APCC_ABBA_1	207	212	PF00400	0.275
LIG_APCC_ABBA_1	301	306	PF00400	0.372
LIG_BRCT_BRCA1_1	23	27	PF00533	0.241
LIG_Clathr_ClatBox_1	88	92	PF01394	0.241
LIG_eIF4E_1	83	89	PF01652	0.384
LIG_FHA_1	264	270	PF00498	0.256
LIG_FHA_1	282	288	PF00498	0.413
LIG_FHA_1	296	302	PF00498	0.287
LIG_FHA_1	306	312	PF00498	0.275
LIG_FHA_1	316	322	PF00498	0.365
LIG_FHA_1	39	45	PF00498	0.289
LIG_FHA_1	68	74	PF00498	0.371
LIG_LIR_Apic_2	118	124	PF02991	0.277
LIG_LIR_Gen_1	236	244	PF02991	0.257
LIG_LIR_Gen_1	294	304	PF02991	0.303
LIG_LIR_Gen_1	306	317	PF02991	0.267
LIG_LIR_LC3C_4	308	313	PF02991	0.353
LIG_LIR_Nem_3	194	200	PF02991	0.280
LIG_LIR_Nem_3	236	241	PF02991	0.257
LIG_LIR_Nem_3	24	30	PF02991	0.241
LIG_LIR_Nem_3	294	300	PF02991	0.337
LIG_LIR_Nem_3	306	312	PF02991	0.249
LIG_LIR_Nem_3	78	84	PF02991	0.244
LIG_LYPXL_SIV_4	199	207	PF13949	0.275
LIG_PDZ_Class_3	348	353	PF00595	0.436
LIG_Pex14_2	56	60	PF04695	0.384
LIG_RPA_C_Fungi	320	332	PF08784	0.463
LIG_SH2_CRK	200	204	PF00017	0.241
LIG_SH2_GRB2like	72	75	PF00017	0.317
LIG_SH2_PTP2	309	312	PF00017	0.231
LIG_SH2_PTP2	83	86	PF00017	0.384
LIG_SH2_STAP1	200	204	PF00017	0.259
LIG_SH2_STAT5	129	132	PF00017	0.241
LIG_SH2_STAT5	197	200	PF00017	0.280
LIG_SH2_STAT5	30	33	PF00017	0.241
LIG_SH2_STAT5	309	312	PF00017	0.231
LIG_SH2_STAT5	68	71	PF00017	0.303
LIG_SH2_STAT5	72	75	PF00017	0.377
LIG_SH2_STAT5	79	82	PF00017	0.334
LIG_SH2_STAT5	83	86	PF00017	0.404
LIG_SUMO_SIM_anti_2	308	314	PF11976	0.278
LIG_SUMO_SIM_par_1	308	314	PF11976	0.280
LIG_SUMO_SIM_par_1	40	45	PF11976	0.349
LIG_TYR_ITIM	198	203	PF00017	0.275
LIG_UBA3_1	149	158	PF00899	0.284
MOD_CDK_SPK_2	138	143	PF00069	0.317
MOD_CDK_SPxxK_3	138	145	PF00069	0.277
MOD_CK1_1	112	118	PF00069	0.412
MOD_CK1_1	190	196	PF00069	0.266
MOD_CK1_1	291	297	PF00069	0.452
MOD_GlcNHglycan	105	108	PF01048	0.159
MOD_GSK3_1	112	119	PF00069	0.443
MOD_GSK3_1	17	24	PF00069	0.241
MOD_GSK3_1	186	193	PF00069	0.282
MOD_GSK3_1	199	206	PF00069	0.296
MOD_GSK3_1	246	253	PF00069	0.371
MOD_GSK3_1	263	270	PF00069	0.307
MOD_GSK3_1	291	298	PF00069	0.361
MOD_GSK3_1	311	318	PF00069	0.315
MOD_GSK3_1	338	345	PF00069	0.439
MOD_GSK3_1	40	47	PF00069	0.293
MOD_N-GLC_1	115	120	PF02516	0.301
MOD_N-GLC_1	246	251	PF02516	0.236
MOD_N-GLC_1	281	286	PF02516	0.361
MOD_NEK2_1	186	191	PF00069	0.256
MOD_NEK2_1	198	203	PF00069	0.325
MOD_NEK2_1	214	219	PF00069	0.112
MOD_NEK2_1	276	281	PF00069	0.314
MOD_NEK2_1	38	43	PF00069	0.278
MOD_NEK2_1	44	49	PF00069	0.287
MOD_NEK2_2	192	197	PF00069	0.241
MOD_PIKK_1	44	50	PF00454	0.275
MOD_PKA_2	234	240	PF00069	0.241
MOD_PKA_2	264	270	PF00069	0.246
MOD_PKA_2	97	103	PF00069	0.275
MOD_Plk_1	233	239	PF00069	0.282
MOD_Plk_1	282	288	PF00069	0.354
MOD_Plk_1	305	311	PF00069	0.298
MOD_Plk_1	44	50	PF00069	0.256
MOD_Plk_4	187	193	PF00069	0.284
MOD_Plk_4	199	205	PF00069	0.275
MOD_Plk_4	234	240	PF00069	0.241
MOD_Plk_4	305	311	PF00069	0.315
MOD_ProDKin_1	138	144	PF00069	0.280
MOD_SUMO_rev_2	115	124	PF00179	0.346
TRG_DiLeu_BaEn_2	131	137	PF01217	0.365
TRG_DiLeu_BaEn_2	233	239	PF01217	0.256
TRG_ENDOCYTIC_2	200	203	PF00928	0.275
TRG_ENDOCYTIC_2	309	312	PF00928	0.231
TRG_ENDOCYTIC_2	83	86	PF00928	0.384
TRG_ER_diArg_1	332	334	PF00400	0.490
TRG_NES_CRM1_1	78	92	PF08389	0.241
TRG_Pf-PMV_PEXEL_1	135	139	PF00026	0.334

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PEZ9	Leptomonas seymouri	83%	99%
A0A0S4INT4	Bodo saltans	45%	93%
A0A1X0NJF6	Trypanosomatidae	66%	100%
A0A3R7MBW5	Trypanosoma rangeli	61%	96%
A0A3S7WSQ4	Leishmania donovani	92%	97%
A4HVT5	Leishmania infantum	92%	97%
D0A6F6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	55%	99%
E9API0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	93%	98%
O74824	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	36%	100%
Q00582	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	35%	100%
Q3SX43	Bos taurus	36%	100%
Q4QFZ8	Leishmania major	91%	100%
Q54IK1	Dictyostelium discoideum	36%	100%
Q5VZM2	Homo sapiens	33%	94%
Q63486	Rattus norvegicus	36%	100%
Q63487	Rattus norvegicus	33%	94%
Q6NTA4	Mus musculus	33%	94%
Q7L523	Homo sapiens	36%	100%
Q80X95	Mus musculus	36%	100%
V5BM39	Trypanosoma cruzi	62%	97%