PseudoU_synth_1 domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

tRNA pseudouridine synthase, putative

Species:

Leishmania braziliensis

UniProt:

A4H7D9_LEIBR

TriTrypDb:

LbrM.13.1350 , LBRM2903_200005100

Length:

764

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 8
NetGPI	no	yes: 0, no: 8

Cellular components
Term	Name	Level	Count
GO:0043226	organelle	2	6
GO:0043227	membrane-bounded organelle	3	6
GO:0043229	intracellular organelle	3	6
GO:0043231	intracellular membrane-bounded organelle	4	6
GO:0110165	cellular anatomical entity	1	6
GO:0005634	nucleus	5	1
GO:0005737	cytoplasm	2	1

Expansion

Sequence features

A4H7D9

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H7D9

Function

Biological processes
Term	Name	Level	Count
GO:0001522	pseudouridine synthesis	6	9
GO:0006139	nucleobase-containing compound metabolic process	3	9
GO:0006396	RNA processing	6	9
GO:0006399	tRNA metabolic process	7	9
GO:0006400	tRNA modification	6	6
GO:0006725	cellular aromatic compound metabolic process	3	9
GO:0006807	nitrogen compound metabolic process	2	9
GO:0008033	tRNA processing	8	9
GO:0008152	metabolic process	1	9
GO:0009451	RNA modification	5	9
GO:0009987	cellular process	1	9
GO:0016070	RNA metabolic process	5	9
GO:0031119	tRNA pseudouridine synthesis	7	6
GO:0034470	ncRNA processing	7	9
GO:0034641	cellular nitrogen compound metabolic process	3	9
GO:0034660	ncRNA metabolic process	6	9
GO:0043170	macromolecule metabolic process	3	9
GO:0043412	macromolecule modification	4	9
GO:0044237	cellular metabolic process	2	9
GO:0044238	primary metabolic process	2	9
GO:0046483	heterocycle metabolic process	3	9
GO:0071704	organic substance metabolic process	2	9
GO:0090304	nucleic acid metabolic process	4	9
GO:1901360	organic cyclic compound metabolic process	3	9
GO:0016071	mRNA metabolic process	6	1
GO:0016556	mRNA modification	6	1
GO:1990481	mRNA pseudouridine synthesis	7	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	9
GO:0003723	RNA binding	4	9
GO:0003824	catalytic activity	1	9
GO:0005488	binding	1	9
GO:0009982	pseudouridine synthase activity	4	9
GO:0016853	isomerase activity	2	9
GO:0016866	intramolecular transferase activity	3	9
GO:0097159	organic cyclic compound binding	2	9
GO:1901363	heterocyclic compound binding	2	9

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	238	242	PF00656	0.446
CLV_C14_Caspase3-7	437	441	PF00656	0.749
CLV_NRD_NRD_1	119	121	PF00675	0.450
CLV_NRD_NRD_1	211	213	PF00675	0.421
CLV_NRD_NRD_1	282	284	PF00675	0.388
CLV_NRD_NRD_1	345	347	PF00675	0.702
CLV_NRD_NRD_1	352	354	PF00675	0.732
CLV_NRD_NRD_1	361	363	PF00675	0.725
CLV_NRD_NRD_1	455	457	PF00675	0.493
CLV_NRD_NRD_1	502	504	PF00675	0.360
CLV_NRD_NRD_1	51	53	PF00675	0.497
CLV_NRD_NRD_1	641	643	PF00675	0.307
CLV_NRD_NRD_1	666	668	PF00675	0.400
CLV_NRD_NRD_1	671	673	PF00675	0.400
CLV_NRD_NRD_1	694	696	PF00675	0.509
CLV_NRD_NRD_1	728	730	PF00675	0.441
CLV_PCSK_FUR_1	117	121	PF00082	0.554
CLV_PCSK_FUR_1	695	699	PF00082	0.333
CLV_PCSK_KEX2_1	116	118	PF00082	0.529
CLV_PCSK_KEX2_1	119	121	PF00082	0.434
CLV_PCSK_KEX2_1	210	212	PF00082	0.539
CLV_PCSK_KEX2_1	250	252	PF00082	0.377
CLV_PCSK_KEX2_1	282	284	PF00082	0.444
CLV_PCSK_KEX2_1	345	347	PF00082	0.581
CLV_PCSK_KEX2_1	361	363	PF00082	0.701
CLV_PCSK_KEX2_1	454	456	PF00082	0.497
CLV_PCSK_KEX2_1	50	52	PF00082	0.495
CLV_PCSK_KEX2_1	502	504	PF00082	0.364
CLV_PCSK_KEX2_1	671	673	PF00082	0.400
CLV_PCSK_KEX2_1	694	696	PF00082	0.509
CLV_PCSK_KEX2_1	697	699	PF00082	0.553
CLV_PCSK_KEX2_1	727	729	PF00082	0.419
CLV_PCSK_PC1ET2_1	116	118	PF00082	0.554
CLV_PCSK_PC1ET2_1	210	212	PF00082	0.520
CLV_PCSK_PC1ET2_1	250	252	PF00082	0.470
CLV_PCSK_PC1ET2_1	361	363	PF00082	0.800
CLV_PCSK_PC1ET2_1	454	456	PF00082	0.497
CLV_PCSK_PC1ET2_1	697	699	PF00082	0.354
CLV_PCSK_PC7_1	451	457	PF00082	0.489
CLV_PCSK_PC7_1	667	673	PF00082	0.400
CLV_PCSK_SKI1_1	155	159	PF00082	0.452
CLV_PCSK_SKI1_1	250	254	PF00082	0.371
CLV_PCSK_SKI1_1	332	336	PF00082	0.742
CLV_PCSK_SKI1_1	363	367	PF00082	0.796
CLV_PCSK_SKI1_1	502	506	PF00082	0.352
CLV_PCSK_SKI1_1	63	67	PF00082	0.492
CLV_PCSK_SKI1_1	662	666	PF00082	0.414
CLV_PCSK_SKI1_1	694	698	PF00082	0.330
CLV_PCSK_SKI1_1	752	756	PF00082	0.476
CLV_Separin_Metazoa	625	629	PF03568	0.307
DEG_Nend_UBRbox_4	1	3	PF02207	0.502
DEG_ODPH_VHL_1	308	320	PF01847	0.540
DEG_SCF_FBW7_2	382	389	PF00400	0.648
DOC_CYCLIN_RxL_1	152	161	PF00134	0.450
DOC_CYCLIN_RxL_1	499	509	PF00134	0.475
DOC_MAPK_gen_1	116	126	PF00069	0.411
DOC_MAPK_gen_1	36	44	PF00069	0.494
DOC_MAPK_gen_1	727	735	PF00069	0.395
DOC_MAPK_gen_1	749	757	PF00069	0.381
DOC_MAPK_MEF2A_6	119	128	PF00069	0.363
DOC_MAPK_MEF2A_6	193	202	PF00069	0.336
DOC_MAPK_MEF2A_6	727	735	PF00069	0.360
DOC_MAPK_RevD_3	654	668	PF00069	0.220
DOC_PP1_RVXF_1	314	321	PF00149	0.650
DOC_PP1_RVXF_1	754	760	PF00149	0.489
DOC_PP2B_LxvP_1	598	601	PF13499	0.359
DOC_PP4_FxxP_1	562	565	PF00568	0.400
DOC_USP7_MATH_1	58	62	PF00917	0.698
DOC_USP7_MATH_1	65	69	PF00917	0.663
DOC_USP7_MATH_1	83	87	PF00917	0.692
DOC_WW_Pin1_4	101	106	PF00397	0.511
DOC_WW_Pin1_4	23	28	PF00397	0.465
DOC_WW_Pin1_4	382	387	PF00397	0.754
DOC_WW_Pin1_4	4	9	PF00397	0.476
DOC_WW_Pin1_4	515	520	PF00397	0.307
DOC_WW_Pin1_4	541	546	PF00397	0.379
DOC_WW_Pin1_4	561	566	PF00397	0.162
DOC_WW_Pin1_4	66	71	PF00397	0.477
LIG_14-3-3_CanoR_1	181	189	PF00244	0.386
LIG_14-3-3_CanoR_1	193	199	PF00244	0.332
LIG_14-3-3_CanoR_1	36	44	PF00244	0.494
LIG_14-3-3_CanoR_1	533	538	PF00244	0.345
LIG_14-3-3_CanoR_1	568	574	PF00244	0.400
LIG_14-3-3_CanoR_1	63	68	PF00244	0.489
LIG_Actin_WH2_2	301	318	PF00022	0.612
LIG_Actin_WH2_2	654	669	PF00022	0.220
LIG_Actin_WH2_2	741	758	PF00022	0.477
LIG_AP2alpha_2	539	541	PF02296	0.331
LIG_BRCT_BRCA1_1	537	541	PF00533	0.342
LIG_Clathr_ClatBox_1	176	180	PF01394	0.476
LIG_deltaCOP1_diTrp_1	555	562	PF00928	0.322
LIG_EH1_1	170	178	PF00400	0.306
LIG_FHA_1	158	164	PF00498	0.388
LIG_FHA_1	226	232	PF00498	0.519
LIG_FHA_1	288	294	PF00498	0.385
LIG_FHA_1	37	43	PF00498	0.493
LIG_FHA_1	383	389	PF00498	0.741
LIG_FHA_1	417	423	PF00498	0.549
LIG_FHA_1	580	586	PF00498	0.389
LIG_FHA_1	709	715	PF00498	0.512
LIG_FHA_2	146	152	PF00498	0.377
LIG_FHA_2	16	22	PF00498	0.492
LIG_FHA_2	293	299	PF00498	0.478
LIG_FHA_2	312	318	PF00498	0.415
LIG_FHA_2	335	341	PF00498	0.739
LIG_FHA_2	412	418	PF00498	0.547
LIG_FHA_2	570	576	PF00498	0.400
LIG_GBD_Chelix_1	731	739	PF00786	0.343
LIG_LIR_Apic_2	285	289	PF02991	0.348
LIG_LIR_Gen_1	165	176	PF02991	0.497
LIG_LIR_Gen_1	290	299	PF02991	0.380
LIG_LIR_Gen_1	468	477	PF02991	0.465
LIG_LIR_Gen_1	511	519	PF02991	0.315
LIG_LIR_Gen_1	685	692	PF02991	0.339
LIG_LIR_Nem_3	104	109	PF02991	0.523
LIG_LIR_Nem_3	133	138	PF02991	0.349
LIG_LIR_Nem_3	145	149	PF02991	0.401
LIG_LIR_Nem_3	165	171	PF02991	0.254
LIG_LIR_Nem_3	270	276	PF02991	0.388
LIG_LIR_Nem_3	511	517	PF02991	0.315
LIG_LIR_Nem_3	527	532	PF02991	0.307
LIG_LIR_Nem_3	685	689	PF02991	0.307
LIG_LRP6_Inhibitor_1	656	662	PF00058	0.220
LIG_LYPXL_yS_3	146	149	PF13949	0.422
LIG_MYND_1	94	98	PF01753	0.635
LIG_PCNA_yPIPBox_3	170	184	PF02747	0.480
LIG_PTAP_UEV_1	327	332	PF05743	0.543
LIG_SH2_CRK	135	139	PF00017	0.353
LIG_SH2_CRK	264	268	PF00017	0.451
LIG_SH2_CRK	529	533	PF00017	0.410
LIG_SH2_GRB2like	276	279	PF00017	0.482
LIG_SH2_GRB2like	514	517	PF00017	0.307
LIG_SH2_NCK_1	204	208	PF00017	0.305
LIG_SH2_SRC	204	207	PF00017	0.486
LIG_SH2_STAP1	284	288	PF00017	0.344
LIG_SH2_STAP1	299	303	PF00017	0.424
LIG_SH2_STAP1	458	462	PF00017	0.660
LIG_SH2_STAP1	573	577	PF00017	0.400
LIG_SH2_STAT5	130	133	PF00017	0.334
LIG_SH2_STAT5	135	138	PF00017	0.344
LIG_SH2_STAT5	14	17	PF00017	0.492
LIG_SH2_STAT5	276	279	PF00017	0.428
LIG_SH2_STAT5	287	290	PF00017	0.303
LIG_SH2_STAT5	303	306	PF00017	0.463
LIG_SH2_STAT5	462	465	PF00017	0.459
LIG_SH2_STAT5	686	689	PF00017	0.348
LIG_SH3_1	672	678	PF00018	0.400
LIG_SH3_1	92	98	PF00018	0.666
LIG_SH3_3	141	147	PF00018	0.442
LIG_SH3_3	197	203	PF00018	0.337
LIG_SH3_3	307	313	PF00018	0.526
LIG_SH3_3	325	331	PF00018	0.613
LIG_SH3_3	415	421	PF00018	0.703
LIG_SH3_3	5	11	PF00018	0.477
LIG_SH3_3	534	540	PF00018	0.308
LIG_SH3_3	672	678	PF00018	0.307
LIG_SH3_3	88	94	PF00018	0.693
LIG_SH3_3	95	101	PF00018	0.632
LIG_SUMO_SIM_anti_2	197	202	PF11976	0.430
LIG_SUMO_SIM_par_1	710	715	PF11976	0.511
LIG_TRAF2_1	148	151	PF00917	0.356
LIG_TRAF2_1	215	218	PF00917	0.502
LIG_TRAF2_1	245	248	PF00917	0.453
LIG_TRAF2_1	295	298	PF00917	0.382
LIG_TRAF2_1	336	339	PF00917	0.713
LIG_TRAF2_1	544	547	PF00917	0.433
LIG_TRAF2_1	607	610	PF00917	0.220
LIG_TRAF2_1	79	82	PF00917	0.709
LIG_UBA3_1	637	643	PF00899	0.307
LIG_WRC_WIRS_1	288	293	PF05994	0.349
MOD_CDK_SPxK_1	101	107	PF00069	0.541
MOD_CDK_SPxK_1	4	10	PF00069	0.479
MOD_CDK_SPxxK_3	515	522	PF00069	0.307
MOD_CDK_SPxxK_3	561	568	PF00069	0.400
MOD_CK1_1	26	32	PF00069	0.510
MOD_CK1_1	292	298	PF00069	0.369
MOD_CK1_1	311	317	PF00069	0.615
MOD_CK1_1	384	390	PF00069	0.613
MOD_CK1_1	411	417	PF00069	0.696
MOD_CK1_1	438	444	PF00069	0.682
MOD_CK1_1	603	609	PF00069	0.220
MOD_CK2_1	145	151	PF00069	0.389
MOD_CK2_1	15	21	PF00069	0.488
MOD_CK2_1	242	248	PF00069	0.511
MOD_CK2_1	292	298	PF00069	0.417
MOD_CK2_1	311	317	PF00069	0.461
MOD_CK2_1	334	340	PF00069	0.750
MOD_CK2_1	392	398	PF00069	0.673
MOD_CK2_1	407	413	PF00069	0.734
MOD_CK2_1	431	437	PF00069	0.689
MOD_CK2_1	438	444	PF00069	0.612
MOD_CK2_1	541	547	PF00069	0.427
MOD_CK2_1	561	567	PF00069	0.232
MOD_CK2_1	569	575	PF00069	0.323
MOD_Cter_Amidation	280	283	PF01082	0.449
MOD_Cter_Amidation	351	354	PF01082	0.549
MOD_GlcNHglycan	328	331	PF01048	0.618
MOD_GlcNHglycan	350	353	PF01048	0.736
MOD_GlcNHglycan	45	48	PF01048	0.495
MOD_GlcNHglycan	54	57	PF01048	0.740
MOD_GlcNHglycan	688	692	PF01048	0.436
MOD_GlcNHglycan	706	709	PF01048	0.438
MOD_GSK3_1	157	164	PF00069	0.387
MOD_GSK3_1	26	33	PF00069	0.510
MOD_GSK3_1	299	306	PF00069	0.527
MOD_GSK3_1	364	371	PF00069	0.791
MOD_GSK3_1	392	399	PF00069	0.724
MOD_GSK3_1	407	414	PF00069	0.590
MOD_GSK3_1	431	438	PF00069	0.718
MOD_GSK3_1	52	59	PF00069	0.741
MOD_GSK3_1	520	527	PF00069	0.344
MOD_GSK3_1	575	582	PF00069	0.410
MOD_GSK3_1	704	711	PF00069	0.394
MOD_N-GLC_1	515	520	PF02516	0.307
MOD_N-GLC_1	704	709	PF02516	0.483
MOD_NEK2_1	28	33	PF00069	0.515
MOD_NEK2_1	407	412	PF00069	0.582
MOD_NEK2_1	470	475	PF00069	0.598
MOD_NEK2_1	476	481	PF00069	0.548
MOD_NEK2_1	585	590	PF00069	0.220
MOD_NEK2_1	682	687	PF00069	0.307
MOD_NEK2_2	524	529	PF00069	0.400
MOD_NEK2_2	58	63	PF00069	0.499
MOD_NEK2_2	747	752	PF00069	0.356
MOD_PIKK_1	26	32	PF00454	0.510
MOD_PIKK_1	308	314	PF00454	0.540
MOD_PIKK_1	435	441	PF00454	0.755
MOD_PIKK_1	722	728	PF00454	0.289
MOD_PK_1	533	539	PF00069	0.220
MOD_PKA_1	361	367	PF00069	0.542
MOD_PKA_1	51	57	PF00069	0.499
MOD_PKA_2	180	186	PF00069	0.388
MOD_PKA_2	361	367	PF00069	0.736
MOD_PKA_2	450	456	PF00069	0.645
MOD_PKA_2	51	57	PF00069	0.499
MOD_PKA_2	567	573	PF00069	0.220
MOD_PKB_1	50	58	PF00069	0.498
MOD_Plk_1	524	530	PF00069	0.410
MOD_Plk_2-3	145	151	PF00069	0.468
MOD_Plk_2-3	232	238	PF00069	0.496
MOD_Plk_2-3	431	437	PF00069	0.618
MOD_Plk_2-3	575	581	PF00069	0.408
MOD_Plk_4	299	305	PF00069	0.534
MOD_Plk_4	470	476	PF00069	0.557
MOD_Plk_4	632	638	PF00069	0.307
MOD_Plk_4	682	688	PF00069	0.307
MOD_ProDKin_1	101	107	PF00069	0.509
MOD_ProDKin_1	23	29	PF00069	0.466
MOD_ProDKin_1	382	388	PF00069	0.756
MOD_ProDKin_1	4	10	PF00069	0.479
MOD_ProDKin_1	515	521	PF00069	0.307
MOD_ProDKin_1	541	547	PF00069	0.379
MOD_ProDKin_1	561	567	PF00069	0.162
MOD_ProDKin_1	66	72	PF00069	0.477
MOD_SUMO_rev_2	245	252	PF00179	0.476
MOD_SUMO_rev_2	81	86	PF00179	0.717
TRG_DiLeu_BaEn_1	248	253	PF01217	0.401
TRG_DiLeu_LyEn_5	248	253	PF01217	0.401
TRG_ENDOCYTIC_2	108	111	PF00928	0.525
TRG_ENDOCYTIC_2	135	138	PF00928	0.344
TRG_ENDOCYTIC_2	146	149	PF00928	0.386
TRG_ENDOCYTIC_2	264	267	PF00928	0.355
TRG_ENDOCYTIC_2	514	517	PF00928	0.314
TRG_ENDOCYTIC_2	529	532	PF00928	0.307
TRG_ENDOCYTIC_2	686	689	PF00928	0.348
TRG_ER_diArg_1	117	120	PF00400	0.559
TRG_ER_diArg_1	211	213	PF00400	0.418
TRG_ER_diArg_1	282	284	PF00400	0.444
TRG_ER_diArg_1	455	457	PF00400	0.493
TRG_ER_diArg_1	49	52	PF00400	0.498
TRG_ER_diArg_1	501	503	PF00400	0.359
TRG_ER_diArg_1	670	672	PF00400	0.400
TRG_ER_diArg_1	694	696	PF00400	0.509
TRG_ER_diArg_1	727	729	PF00400	0.480
TRG_NLS_Bipartite_1	345	365	PF00514	0.718
TRG_NLS_MonoExtC_3	115	120	PF00514	0.562
TRG_NLS_MonoExtC_3	453	459	PF00514	0.489
TRG_NLS_MonoExtN_4	117	124	PF00514	0.422
TRG_NLS_MonoExtN_4	345	350	PF00514	0.710
TRG_NLS_MonoExtN_4	360	365	PF00514	0.701
TRG_NLS_MonoExtN_4	451	458	PF00514	0.490
TRG_Pf-PMV_PEXEL_1	155	159	PF00026	0.452
TRG_Pf-PMV_PEXEL_1	502	507	PF00026	0.479

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I609	Leptomonas seymouri	67%	100%
A0A3Q8IKM7	Leishmania donovani	80%	100%
A4I9K1	Leishmania infantum	80%	100%
D0A1W0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	45%	100%
E9B4J9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	80%	100%
Q4Q3H7	Leishmania major	81%	100%
V5B271	Trypanosoma cruzi	45%	100%