Putative N(2), N(2)-dimethylguanosine tRNA methyltransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

N(2), N(2)-dimethylguanosine tRNA methyltransferase, putative

Species:

Leishmania braziliensis

UniProt:

A4H782_LEIBR

TriTrypDb:

LbrM.13.0750 , LBRM2903_130013300

Length:

734

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4H782

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H782

Function

Biological processes
Term	Name	Level	Count
GO:0001510	RNA methylation	4	1
GO:0002940	tRNA N2-guanine methylation	6	1
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006396	RNA processing	6	1
GO:0006399	tRNA metabolic process	7	1
GO:0006400	tRNA modification	6	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008033	tRNA processing	8	1
GO:0008152	metabolic process	1	1
GO:0009451	RNA modification	5	1
GO:0009987	cellular process	1	1
GO:0016070	RNA metabolic process	5	1
GO:0030488	tRNA methylation	5	1
GO:0032259	methylation	2	1
GO:0034470	ncRNA processing	7	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0034660	ncRNA metabolic process	6	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0043414	macromolecule methylation	3	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044260	obsolete cellular macromolecule metabolic process	3	1
GO:0046483	heterocycle metabolic process	3	1
GO:0071704	organic substance metabolic process	2	1
GO:0090304	nucleic acid metabolic process	4	1
GO:1901360	organic cyclic compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0000049	tRNA binding	5	12
GO:0003676	nucleic acid binding	3	12
GO:0003723	RNA binding	4	12
GO:0003824	catalytic activity	1	12
GO:0004809	tRNA (guanine-N2-)-methyltransferase activity	6	12
GO:0005488	binding	1	12
GO:0008168	methyltransferase activity	4	12
GO:0008170	N-methyltransferase activity	5	12
GO:0008173	RNA methyltransferase activity	4	12
GO:0008175	tRNA methyltransferase activity	5	12
GO:0008757	S-adenosylmethionine-dependent methyltransferase activity	5	12
GO:0016423	tRNA (guanine) methyltransferase activity	6	12
GO:0016740	transferase activity	2	12
GO:0016741	transferase activity, transferring one-carbon groups	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140101	catalytic activity, acting on a tRNA	4	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	282	286	PF00656	0.266
CLV_C14_Caspase3-7	459	463	PF00656	0.677
CLV_C14_Caspase3-7	58	62	PF00656	0.652
CLV_C14_Caspase3-7	649	653	PF00656	0.603
CLV_C14_Caspase3-7	725	729	PF00656	0.709
CLV_MEL_PAP_1	189	195	PF00089	0.403
CLV_NRD_NRD_1	397	399	PF00675	0.280
CLV_NRD_NRD_1	465	467	PF00675	0.700
CLV_NRD_NRD_1	707	709	PF00675	0.581
CLV_NRD_NRD_1	99	101	PF00675	0.286
CLV_PCSK_KEX2_1	225	227	PF00082	0.493
CLV_PCSK_KEX2_1	396	398	PF00082	0.276
CLV_PCSK_KEX2_1	446	448	PF00082	0.831
CLV_PCSK_KEX2_1	706	708	PF00082	0.573
CLV_PCSK_KEX2_1	98	100	PF00082	0.302
CLV_PCSK_PC1ET2_1	225	227	PF00082	0.464
CLV_PCSK_PC1ET2_1	446	448	PF00082	0.833
CLV_PCSK_PC7_1	393	399	PF00082	0.421
CLV_PCSK_PC7_1	442	448	PF00082	0.676
CLV_PCSK_SKI1_1	125	129	PF00082	0.365
CLV_PCSK_SKI1_1	243	247	PF00082	0.456
CLV_PCSK_SKI1_1	271	275	PF00082	0.300
CLV_PCSK_SKI1_1	317	321	PF00082	0.266
CLV_PCSK_SKI1_1	347	351	PF00082	0.337
CLV_PCSK_SKI1_1	533	537	PF00082	0.364
CLV_PCSK_SKI1_1	563	567	PF00082	0.358
CLV_PCSK_SKI1_1	627	631	PF00082	0.300
CLV_PCSK_SKI1_1	666	670	PF00082	0.589
DEG_APCC_DBOX_1	316	324	PF00400	0.281
DEG_APCC_DBOX_1	532	540	PF00400	0.189
DEG_APCC_DBOX_1	562	570	PF00400	0.411
DEG_APCC_KENBOX_2	162	166	PF00400	0.344
DEG_SCF_TRCP1_1	649	654	PF00400	0.491
DOC_ANK_TNKS_1	686	693	PF00023	0.441
DOC_CKS1_1	230	235	PF01111	0.508
DOC_CKS1_1	590	595	PF01111	0.360
DOC_CYCLIN_RxL_1	240	249	PF00134	0.411
DOC_CYCLIN_RxL_1	312	324	PF00134	0.266
DOC_CYCLIN_yCln2_LP_2	521	527	PF00134	0.325
DOC_MAPK_DCC_7	331	340	PF00069	0.263
DOC_MAPK_gen_1	271	281	PF00069	0.281
DOC_MAPK_gen_1	329	337	PF00069	0.266
DOC_MAPK_gen_1	351	361	PF00069	0.210
DOC_MAPK_MEF2A_6	186	195	PF00069	0.421
DOC_MAPK_MEF2A_6	331	340	PF00069	0.263
DOC_PP2B_LxvP_1	32	35	PF13499	0.426
DOC_PP2B_LxvP_1	669	672	PF13499	0.307
DOC_PP4_FxxP_1	70	73	PF00568	0.266
DOC_USP7_MATH_1	216	220	PF00917	0.550
DOC_USP7_MATH_1	234	238	PF00917	0.636
DOC_USP7_MATH_1	258	262	PF00917	0.316
DOC_USP7_MATH_1	389	393	PF00917	0.331
DOC_USP7_MATH_1	496	500	PF00917	0.620
DOC_USP7_MATH_1	659	663	PF00917	0.645
DOC_USP7_MATH_2	233	239	PF00917	0.388
DOC_USP7_UBL2_3	182	186	PF12436	0.480
DOC_WW_Pin1_4	187	192	PF00397	0.688
DOC_WW_Pin1_4	229	234	PF00397	0.654
DOC_WW_Pin1_4	260	265	PF00397	0.314
DOC_WW_Pin1_4	470	475	PF00397	0.581
DOC_WW_Pin1_4	480	485	PF00397	0.466
DOC_WW_Pin1_4	589	594	PF00397	0.360
DOC_WW_Pin1_4	655	660	PF00397	0.710
LIG_14-3-3_CanoR_1	381	387	PF00244	0.411
LIG_14-3-3_CanoR_1	506	514	PF00244	0.189
LIG_Actin_WH2_2	103	121	PF00022	0.281
LIG_Actin_WH2_2	78	95	PF00022	0.331
LIG_APCC_ABBA_1	171	176	PF00400	0.510
LIG_APCC_ABBA_1	338	343	PF00400	0.266
LIG_BIR_II_1	1	5	PF00653	0.570
LIG_BIR_III_4	637	641	PF00653	0.452
LIG_BRCT_BRCA1_1	4	8	PF00533	0.393
LIG_CtBP_PxDLS_1	52	56	PF00389	0.484
LIG_FHA_1	26	32	PF00498	0.392
LIG_FHA_1	557	563	PF00498	0.369
LIG_FHA_1	590	596	PF00498	0.325
LIG_FHA_2	106	112	PF00498	0.266
LIG_FHA_2	159	165	PF00498	0.426
LIG_FHA_2	167	173	PF00498	0.442
LIG_FHA_2	230	236	PF00498	0.585
LIG_FHA_2	280	286	PF00498	0.266
LIG_FHA_2	562	568	PF00498	0.264
LIG_FHA_2	672	678	PF00498	0.289
LIG_GSK3_LRP6_1	589	594	PF00069	0.271
LIG_IRF3_LxIS_1	77	84	PF10401	0.403
LIG_LIR_Apic_2	376	382	PF02991	0.331
LIG_LIR_Gen_1	583	591	PF02991	0.267
LIG_LIR_Gen_1	87	97	PF02991	0.325
LIG_LIR_Nem_3	267	273	PF02991	0.381
LIG_LIR_Nem_3	583	587	PF02991	0.306
LIG_LIR_Nem_3	87	92	PF02991	0.325
LIG_LYPXL_yS_3	159	162	PF13949	0.551
LIG_MYND_1	240	244	PF01753	0.427
LIG_NRBOX	535	541	PF00104	0.266
LIG_PCNA_yPIPBox_3	243	254	PF02747	0.329
LIG_PTB_Apo_2	126	133	PF02174	0.411
LIG_PTB_Apo_2	146	153	PF02174	0.411
LIG_PTB_Phospho_1	126	132	PF10480	0.411
LIG_PTB_Phospho_1	146	152	PF10480	0.411
LIG_REV1ctd_RIR_1	347	355	PF16727	0.325
LIG_SH2_CRK	152	156	PF00017	0.411
LIG_SH2_CRK	353	357	PF00017	0.281
LIG_SH2_CRK	526	530	PF00017	0.281
LIG_SH2_NCK_1	526	530	PF00017	0.266
LIG_SH2_NCK_1	71	75	PF00017	0.266
LIG_SH2_PTP2	333	336	PF00017	0.190
LIG_SH2_PTP2	584	587	PF00017	0.411
LIG_SH2_SRC	132	135	PF00017	0.325
LIG_SH2_STAT3	368	371	PF00017	0.296
LIG_SH2_STAT5	132	135	PF00017	0.343
LIG_SH2_STAT5	333	336	PF00017	0.281
LIG_SH2_STAT5	345	348	PF00017	0.266
LIG_SH2_STAT5	584	587	PF00017	0.386
LIG_SH3_1	695	701	PF00018	0.451
LIG_SH3_2	471	476	PF14604	0.526
LIG_SH3_3	154	160	PF00018	0.511
LIG_SH3_3	227	233	PF00018	0.532
LIG_SH3_3	28	34	PF00018	0.376
LIG_SH3_3	383	389	PF00018	0.189
LIG_SH3_3	468	474	PF00018	0.523
LIG_SH3_3	567	573	PF00018	0.294
LIG_SH3_3	584	590	PF00018	0.199
LIG_SH3_3	695	701	PF00018	0.451
LIG_SUMO_SIM_anti_2	105	111	PF11976	0.360
LIG_SUMO_SIM_anti_2	82	87	PF11976	0.309
LIG_SUMO_SIM_par_1	252	257	PF11976	0.272
LIG_SUMO_SIM_par_1	537	545	PF11976	0.244
LIG_SUMO_SIM_par_1	84	90	PF11976	0.331
LIG_TRAF2_1	434	437	PF00917	0.559
LIG_TRAF2_1	56	59	PF00917	0.634
LIG_TRAF2_1	565	568	PF00917	0.281
LIG_TRFH_1	575	579	PF08558	0.331
LIG_TYR_ITIM	157	162	PF00017	0.456
MOD_CDC14_SPxK_1	473	476	PF00782	0.669
MOD_CDK_SPK_2	187	192	PF00069	0.565
MOD_CDK_SPxK_1	470	476	PF00069	0.614
MOD_CK1_1	187	193	PF00069	0.440
MOD_CK1_1	2	8	PF00069	0.566
MOD_CK1_1	22	28	PF00069	0.563
MOD_CK1_1	283	289	PF00069	0.280
MOD_CK1_1	373	379	PF00069	0.474
MOD_CK1_1	41	47	PF00069	0.458
MOD_CK1_1	475	481	PF00069	0.593
MOD_CK1_1	499	505	PF00069	0.743
MOD_CK2_1	105	111	PF00069	0.266
MOD_CK2_1	158	164	PF00069	0.417
MOD_CK2_1	166	172	PF00069	0.443
MOD_CK2_1	229	235	PF00069	0.444
MOD_CK2_1	53	59	PF00069	0.717
MOD_CK2_1	561	567	PF00069	0.270
MOD_CK2_1	81	87	PF00069	0.403
MOD_Cter_Amidation	223	226	PF01082	0.510
MOD_GlcNHglycan	113	116	PF01048	0.266
MOD_GlcNHglycan	218	222	PF01048	0.502
MOD_GlcNHglycan	237	240	PF01048	0.412
MOD_GlcNHglycan	260	263	PF01048	0.298
MOD_GlcNHglycan	285	288	PF01048	0.322
MOD_GlcNHglycan	361	364	PF01048	0.421
MOD_GlcNHglycan	375	378	PF01048	0.423
MOD_GlcNHglycan	4	7	PF01048	0.397
MOD_GlcNHglycan	429	432	PF01048	0.814
MOD_GlcNHglycan	43	46	PF01048	0.638
MOD_GlcNHglycan	501	504	PF01048	0.627
MOD_GlcNHglycan	509	512	PF01048	0.230
MOD_GlcNHglycan	521	524	PF01048	0.273
MOD_GlcNHglycan	648	652	PF01048	0.539
MOD_GlcNHglycan	724	727	PF01048	0.749
MOD_GSK3_1	22	29	PF00069	0.502
MOD_GSK3_1	279	286	PF00069	0.314
MOD_GSK3_1	37	44	PF00069	0.583
MOD_GSK3_1	647	654	PF00069	0.534
MOD_GSK3_1	655	662	PF00069	0.683
MOD_LATS_1	298	304	PF00433	0.266
MOD_N-GLC_1	187	192	PF02516	0.445
MOD_N-GLC_1	53	58	PF02516	0.739
MOD_NEK2_1	113	118	PF00069	0.280
MOD_NEK2_1	217	222	PF00069	0.398
MOD_NEK2_1	279	284	PF00069	0.279
MOD_NEK2_1	293	298	PF00069	0.266
MOD_NEK2_1	370	375	PF00069	0.333
MOD_NEK2_1	561	566	PF00069	0.270
MOD_NEK2_1	81	86	PF00069	0.403
MOD_NEK2_2	336	341	PF00069	0.266
MOD_NEK2_2	382	387	PF00069	0.295
MOD_PIKK_1	527	533	PF00454	0.281
MOD_PIKK_1	604	610	PF00454	0.266
MOD_PIKK_1	63	69	PF00454	0.314
MOD_PK_1	38	44	PF00069	0.497
MOD_PK_1	79	85	PF00069	0.266
MOD_PKA_1	38	44	PF00069	0.549
MOD_PKA_2	389	395	PF00069	0.373
MOD_PKA_2	475	481	PF00069	0.625
MOD_PKA_2	556	562	PF00069	0.295
MOD_PKA_2	604	610	PF00069	0.281
MOD_PKB_1	98	106	PF00069	0.411
MOD_Plk_1	676	682	PF00069	0.374
MOD_Plk_1	79	85	PF00069	0.276
MOD_Plk_2-3	105	111	PF00069	0.360
MOD_Plk_4	580	586	PF00069	0.266
MOD_ProDKin_1	187	193	PF00069	0.683
MOD_ProDKin_1	229	235	PF00069	0.657
MOD_ProDKin_1	260	266	PF00069	0.314
MOD_ProDKin_1	470	476	PF00069	0.587
MOD_ProDKin_1	480	486	PF00069	0.464
MOD_ProDKin_1	589	595	PF00069	0.360
MOD_ProDKin_1	655	661	PF00069	0.707
MOD_SUMO_rev_2	179	187	PF00179	0.473
MOD_SUMO_rev_2	649	659	PF00179	0.501
TRG_DiLeu_BaLyEn_6	237	242	PF01217	0.487
TRG_DiLeu_BaLyEn_6	333	338	PF01217	0.302
TRG_DiLeu_BaLyEn_6	664	669	PF01217	0.396
TRG_ENDOCYTIC_2	124	127	PF00928	0.289
TRG_ENDOCYTIC_2	152	155	PF00928	0.411
TRG_ENDOCYTIC_2	159	162	PF00928	0.551
TRG_ENDOCYTIC_2	301	304	PF00928	0.266
TRG_ENDOCYTIC_2	584	587	PF00928	0.411
TRG_ENDOCYTIC_2	626	629	PF00928	0.189
TRG_ER_diArg_1	396	398	PF00400	0.360
TRG_ER_diArg_1	585	588	PF00400	0.264
TRG_ER_diArg_1	686	689	PF00400	0.538
TRG_ER_diArg_1	705	708	PF00400	0.708
TRG_ER_diArg_1	91	94	PF00400	0.283
TRG_ER_diArg_1	97	100	PF00400	0.279
TRG_Pf-PMV_PEXEL_1	243	247	PF00026	0.421
TRG_Pf-PMV_PEXEL_1	271	275	PF00026	0.300

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PF43	Leptomonas seymouri	69%	100%
A0A0S4IT52	Bodo saltans	47%	100%
A0A1X0NNC1	Trypanosomatidae	48%	97%
A0A3R7KAT5	Trypanosoma rangeli	51%	100%
A0A3S7WSL8	Leishmania donovani	79%	100%
A4HVM2	Leishmania infantum	79%	100%
D0A6N2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	50%	100%
E9APB8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	81%	100%
Q4QG60	Leishmania major	80%	98%
V5BB27	Trypanosoma cruzi	51%	100%